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Protein

Auxin-induced in root cultures protein 12

Gene

AIR12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One-heme-containing cytochrome.1 Publication

Cofactori

hemeCuratedNote: Binds 1 heme group non-covalently.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron (heme axial ligand)Sequence Analysis
Metal bindingi176 – 1761Iron (heme axial ligand)Sequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. auxin-activated signaling pathway Source: UniProtKB-KW
  2. oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Auxin signaling pathway, Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin-induced in root cultures protein 12
Gene namesi
Name:AIR12
Ordered Locus Names:At3g07390
ORF Names:F21O3_10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G07390.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 228203Auxin-induced in root cultures protein 12PRO_0000020655Add
BLAST
Propeptidei229 – 25224Removed in mature formSequence AnalysisPRO_0000020656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Lipidationi228 – 2281GPI-anchor amidated glycineSequence Analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ94BT2.
PRIDEiQ94BT2.

Expressioni

Developmental stagei

Expressed during auxin-induced lateral root formation.1 Publication

Inductioni

Induced between 4 and 8 hours after treatment with auxin and remains high for at least 24 hours.2 Publications

Gene expression databases

GenevestigatoriQ94BT2.

Structurei

3D structure databases

ProteinModelPortaliQ94BT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 165117DOMONPROSITE-ProRule annotationAdd
BLAST

Domaini

DOMON domain could bind one heme b.1 Publication

Sequence similaritiesi

Contains 1 DOMON domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG307188.
HOGENOMiHOG000239821.
InParanoidiQ94BT2.
PhylomeDBiQ94BT2.

Family and domain databases

InterProiIPR005018. DOMON_domain.
[Graphical view]
PfamiPF04526. DUF568. 1 hit.
[Graphical view]
SMARTiSM00664. DoH. 1 hit.
[Graphical view]
PROSITEiPS50836. DOMON. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94BT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSSSLLI LAVACFVSLI SPAISQQACK SQNLNSAGPF DSCEDLPVLN
60 70 80 90 100
SYLHYTYNSS NSSLSVAFVA TPSQANGGWV AWAINPTGTK MAGSQAFLAY
110 120 130 140 150
RSGGGAAPVV KTYNISSYSS LVEGKLAFDF WNLRAESLSG GRIAIFTTVK
160 170 180 190 200
VPAGADSVNQ VWQIGGNVTN GRPGVHPFGP DNLGSHRVLS FTEDAAPGSA
210 220 230 240 250
PSPGSAPAPG TSGSTTPGTA AGGPGNAGSL TRNVNFGVNL GILVLLGSIF

IF
Length:252
Mass (Da):25,592
Last modified:October 31, 2003 - v3
Checksum:iDB20541FE856E603
GO

Sequence cautioni

The sequence AAC62613.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAK64012.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AEE74537.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471T → NR in AAC62613 (PubMed:10080694).Curated
Sequence conflicti155 – 1551A → R in AAC62613 (PubMed:10080694).Curated
Sequence conflicti207 – 2071P → PSPGSAP in AAC62613 (PubMed:10080694).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055850 mRNA. Translation: AAC62613.1. Different initiation.
AC009853 Genomic DNA. Translation: AAF02148.1.
CP002686 Genomic DNA. Translation: AEE74537.1. Different initiation.
AY039908 mRNA. Translation: AAK64012.2. Different initiation.
AY077679 mRNA. Translation: AAL76157.1.
PIRiT51337.
RefSeqiNP_566306.3. NM_111618.3.
UniGeneiAt.48629.

Genome annotation databases

EnsemblPlantsiAT3G07390.1; AT3G07390.1; AT3G07390.
GeneIDi819927.
KEGGiath:AT3G07390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055850 mRNA. Translation: AAC62613.1. Different initiation.
AC009853 Genomic DNA. Translation: AAF02148.1.
CP002686 Genomic DNA. Translation: AEE74537.1. Different initiation.
AY039908 mRNA. Translation: AAK64012.2. Different initiation.
AY077679 mRNA. Translation: AAL76157.1.
PIRiT51337.
RefSeqiNP_566306.3. NM_111618.3.
UniGeneiAt.48629.

3D structure databases

ProteinModelPortaliQ94BT2.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ94BT2.
PRIDEiQ94BT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G07390.1; AT3G07390.1; AT3G07390.
GeneIDi819927.
KEGGiath:AT3G07390.

Organism-specific databases

TAIRiAT3G07390.

Phylogenomic databases

eggNOGiNOG307188.
HOGENOMiHOG000239821.
InParanoidiQ94BT2.
PhylomeDBiQ94BT2.

Gene expression databases

GenevestigatoriQ94BT2.

Family and domain databases

InterProiIPR005018. DOMON_domain.
[Graphical view]
PfamiPF04526. DUF568. 1 hit.
[Graphical view]
SMARTiSM00664. DoH. 1 hit.
[Graphical view]
PROSITEiPS50836. DOMON. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of cDNA clones corresponding with mRNAs that accumulate during auxin-induced lateral root formation."
    Neuteboom L.W., Ng J.M.Y., Kuyper M., Clijdesdale O.R., Hooykaas P.J.J., van der Zaal B.J.
    Plant Mol. Biol. 39:273-287(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, DEVELOPMENTAL STAGE.
    Strain: cv. C24.
    Tissue: Cultured root.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-252.
    Strain: cv. Columbia.
  5. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  6. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
    Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
    Plant Physiol. 132:568-577(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
    Tissue: Callus.
  7. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  8. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  9. "Expression profiling of auxin-treated Arabidopsis roots: toward a molecular analysis of lateral root emergence."
    Laskowski M., Biller S., Stanley K., Kajstura T., Prusty R.
    Plant Cell Physiol. 47:788-792(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY AUXIN.
  10. "Auxin-responsive genes AIR12 code for a new family of plasma membrane b-type cytochromes specific to flowering plants."
    Preger V., Tango N., Marchand C., Lemaire S.D., Carbonera D., Di Valentin M., Costa A., Pupillo P., Trost P.
    Plant Physiol. 150:606-620(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, FUNCTION, DOMAIN.

Entry informationi

Entry nameiAIR12_ARATH
AccessioniPrimary (citable) accession number: Q94BT2
Secondary accession number(s): F4JEF2, O82442, Q9SRS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: January 7, 2015
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.