Q94BT2 (AIR12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auxin-induced in root cultures protein 12

Gene names

Name:	AIR12
Ordered Locus Names:	At3g07390
ORF Names:	F21O3_10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Developmental stage	Expressed during auxin-induced lateral root formation. Ref.1
Induction	Induced between 4 and 8 hours after treatment with auxin and remains high for at least 24 hours. Ref.1
Sequence similarities	Contains 1 DOMON domain.
Sequence caution	The sequence AAC62613.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAK64012.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AEE74537.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Auxin signaling pathway
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 228	203	Auxin-induced in root cultures protein 12		PRO_0000020655
	Propeptide	229 – 252	24	Removed in mature form Potential		PRO_0000020656
Regions
	Domain	49 – 165	117	DOMON
Amino acid modifications
	Lipidation	228	1	GPI-anchor amidated glycine Potential
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	147	1	T → NR in AAC62613. Ref.1
	Sequence conflict	155	1	A → R in AAC62613. Ref.1
	Sequence conflict	207	1	P → PSPGSAP in AAC62613. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q94BT2 [UniParc]. Last modified October 31, 2003. Version 3. Checksum: DB20541FE856E603	FASTA	252	25,592
	10 20 30 40 50 60 MASSSSSLLI LAVACFVSLI SPAISQQACK SQNLNSAGPF DSCEDLPVLN SYLHYTYNSS 70 80 90 100 110 120 NSSLSVAFVA TPSQANGGWV AWAINPTGTK MAGSQAFLAY RSGGGAAPVV KTYNISSYSS 130 140 150 160 170 180 LVEGKLAFDF WNLRAESLSG GRIAIFTTVK VPAGADSVNQ VWQIGGNVTN GRPGVHPFGP 190 200 210 220 230 240 DNLGSHRVLS FTEDAAPGSA PSPGSAPAPG TSGSTTPGTA AGGPGNAGSL TRNVNFGVNL 250 GILVLLGSIF IF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNA clones corresponding with mRNAs that accumulate during auxin-induced lateral root formation." Neuteboom L.W., Ng J.M.Y., Kuyper M., Clijdesdale O.R., Hooykaas P.J.J., van der Zaal B.J. Plant Mol. Biol. 39:273-287(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, DEVELOPMENTAL STAGE. Strain: cv. C24. Tissue: Cultured root.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-252. Strain: cv. Columbia.
[5]	"Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis." Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P. Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Callus.
[6]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF055850 mRNA. Translation: AAC62613.1. Different initiation. AC009853 Genomic DNA. Translation: AAF02148.1. CP002686 Genomic DNA. Translation: AEE74537.1. Different initiation. AY039908 mRNA. Translation: AAK64012.2. Different initiation. AY077679 mRNA. Translation: AAL76157.1.
IPI	IPI00543636.
PIR	T51337.
RefSeq	NP_566306.3. NM_111618.3.
UniGene	At.48629.
3D structure databases
ModBase	Search...
Proteomic databases
PaxDb	Q94BT2.
PRIDE	Q94BT2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	819927.
KEGG	ath:AT3G07390.
Organism-specific databases
TAIR	At3g07390.
Phylogenomic databases
eggNOG	NOG307188.
HOGENOM	HOG000239821.
InParanoid	Q94BT2.
PhylomeDB	Q94BT2.
Gene expression databases
ArrayExpress	Q94BT2.
Genevestigator	Q94BT2.
GermOnline	AT3G07390. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005018. DOMON_domain. IPR017214. UCP037471. [Graphical view]
Pfam	PF04526. DUF568. 1 hit. [Graphical view]
SMART	SM00664. DoH. 1 hit. [Graphical view]
PROSITE	PS50836. DOMON. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AIR12_ARATH

Accession

Primary (citable) accession number: Q94BT2
Secondary accession number(s): F4JEF2, O82442, Q9SRS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program