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Protein

Sucrose-phosphate synthase 1

Gene

SPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a major role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation. Required for nectar secretion.4 Publications

Miscellaneous

Plants silencing SPS1 show reduced shoot growth, leaf fresh weight and dry weight, and decreased leaf starch, leaf sugar levels and sucrose export rates (PubMed:10998187 and PubMed:21309792). Tobacco plants overexpressing Arabidopsis SPS1 show increased stem height and diameter, increased total dry weight and elevated concentrations of sink sucrose pools (PubMed:17415671).1 Publication

Catalytic activityi

UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate.

Enzyme regulationi

Activity is regulated by phosphorylation and moderated by concentration of metabolites and light.By similarity

Pathwayi: sucrose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable sucrose-phosphate synthase 3 (SPS3), Probable sucrose-phosphate synthase 4 (SPS4), Sucrose-phosphate synthase 1 (SPS1), Probable sucrose-phosphate synthase 2 (SPS2)
  2. Probable sucrose-phosphatase 3b (SPP3B), Probable sucrose-phosphatase 1 (SPP1), Probable sucrose-phosphatase 2 (SPP2), Probable sucrose-phosphatase 3a (SPP3A)
This subpathway is part of the pathway sucrose biosynthesis, which is itself part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose, the pathway sucrose biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • sucrose-phosphate synthase activity Source: TAIR
  • sucrose synthase activity Source: InterPro

GO - Biological processi

  • nectar secretion Source: UniProtKB
  • sucrose biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545

Protein family/group databases

CAZyiGT4 Glycosyltransferase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose-phosphate synthase 1 (EC:2.4.1.14)
Alternative name(s):
Sucrose-phosphate synthase 1F
Short name:
AtSPS1F
Sucrose-phosphate synthase 5.1
Short name:
AtSPS5.1
UDP-glucose-fructose-phosphate glucosyltransferase
Gene namesi
Name:SPS1
Synonyms:SPSA1
Ordered Locus Names:At5g20280
ORF Names:F5O24.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20280
TAIRilocus:2149179 AT5G20280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Loss of nectar secretion accompanied by starch accumulation in nectaries.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004136371 – 1043Sucrose-phosphate synthase 1Add BLAST1043

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei121PhosphoserineCombined sources1
Modified residuei125PhosphoserineCombined sources1
Modified residuei152PhosphoserineCombined sources1 Publication1
Modified residuei155PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-152 upon sucrose supply.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ94BT0
PRIDEiQ94BT0

PTM databases

iPTMnetiQ94BT0

Expressioni

Tissue specificityi

Expressed in seeds, stems, rosette leaves, flowers and siliques. Highly expressed in maturing nectaries.2 Publications

Inductioni

Circadian-regulated, with the highest expression at the end of the light period and the lowest at the end of the dark period (in 12 hours light/12 hours dark cycle). Induced by cold (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ94BT0 baseline and differential
GenevisibleiQ94BT0 AT

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

BioGridi17426, 7 interactors
IntActiQ94BT0, 7 interactors
STRINGi3702.AT5G20280.1

Structurei

3D structure databases

ProteinModelPortaliQ94BT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG0853 Eukaryota
COG0438 LUCA
HOGENOMiHOG000009685
InParanoidiQ94BT0
KOiK00696
OMAiSTHGEST
OrthoDBiEOG093600RM
PhylomeDBiQ94BT0

Family and domain databases

CDDicd16419 HAD_SPS, 1 hit
Gene3Di3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR023214 HAD_sf
IPR006380 SPP_N
IPR035659 SPS_C
IPR000368 Sucrose_synth
IPR012819 SucrsPsyn_pln
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF05116 S6PP, 1 hit
PF00862 Sucrose_synth, 1 hit
TIGRFAMsiTIGR02468 sucrsPsyn_pln, 1 hit

Sequencei

Sequence statusi: Complete.

Q94BT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNDWVNSY LEAILDVGQG LDDARSSPSL LLRERGRFTP SRYFVEEVIT
60 70 80 90 100
GYDETDLHRS WVKAVATRSP QERNTRLENM CWRIWNLARQ KKQHEEKEAQ
110 120 130 140 150
RLAKRRLERE KGRREATADM SEEFSEGEKG DIISDISTHG ESTKPRLPRI
160 170 180 190 200
NSAESMELWA SQQKGNKLYL VLISLHGLIR GENMELGRDS DTGGQVKYVV
210 220 230 240 250
ELARALGSMP GVYRVDLLTR QVSSPDVDYS YGEPTEMLTP RDSEDFSDEM
260 270 280 290 300
GESSGAYIVR IPFGPKDKYI PKELLWPHIP EFVDGAMSHI MQMSNVLGEQ
310 320 330 340 350
VGVGKPIWPS AIHGHYADAG DATALLSGAL NVPMLLTGHS LGRDKLEQLL
360 370 380 390 400
RQGRLSKEEI NSTYKIMRRI EGEELSLDVS EMVITSTRQE IDEQWRLYDG
410 420 430 440 450
FDPILERKLR ARIKRNVSCY GRFMPRMVKI PPGMEFNHIV PHGGDMEDTD
460 470 480 490 500
GNEEHPTSPD PPIWAEIMRF FSNSRKPMIL ALARPDPKKN ITTLVKAFGE
510 520 530 540 550
CRPLRELANL ALIMGNRDGI DEMSSTSSSV LLSVLKLIDK YDLYGQVAYP
560 570 580 590 600
KHHKQSDVPD IYRLAAKSKG VFINPAIIEP FGLTLIEAAA HGLPMVATKN
610 620 630 640 650
GGPVDIHRVL DNGLLVDPHD QQSISEALLK LVADKHLWAK CRQNGLKNIH
660 670 680 690 700
QFSWPEHCKT YLSRITSFKP RHPQWQSDDG GDNSEPESPS DSLRDIQDIS
710 720 730 740 750
LNLKFSFDGS GNDNYMNQEG SSMDRKSKIE AAVQNWSKGK DSRKMGSLER
760 770 780 790 800
SEVNSGKFPA VRRRKFIVVI ALDFDGEEDT LEATKRILDA VEKERAEGSV
810 820 830 840 850
GFILSTSLTI SEVQSFLVSG GLNPNDFDAF ICNSGSDLHY TSLNNEDGPF
860 870 880 890 900
VVDFYYHSHI EYRWGGEGLR KTLIRWASSL NEKKADNDEQ IVTLAEHLST
910 920 930 940 950
DYCYTFTVKK PAAVPPVREL RKLLRIQALR CHVVYSQNGT RINVIPVLAS
960 970 980 990 1000
RIQALRYLFV RWGIDMAKMA VFVGESGDTD YEGLLGGLHK SVVLKGVSCS
1010 1020 1030 1040
ACLHANRSYP LTDVISFESN NVVHASPDSD VRDALKKLEL LKD
Length:1,043
Mass (Da):117,321
Last modified:December 1, 2001 - v1
Checksum:i7E24C7B6AA656FB8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti874I → V in BAF01930 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296825 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92824.1
AY039911 mRNA Translation: AAK64015.1
AY079334 mRNA Translation: AAL85065.1
AK230113 mRNA Translation: BAF01930.1
RefSeqiNP_197528.1, NM_122035.3
UniGeneiAt.22681

Genome annotation databases

EnsemblPlantsiAT5G20280.1; AT5G20280.1; AT5G20280
GeneIDi832150
GrameneiAT5G20280.1; AT5G20280.1; AT5G20280
KEGGiath:AT5G20280

Similar proteinsi

Entry informationi

Entry nameiSPSA1_ARATH
AccessioniPrimary (citable) accession number: Q94BT0
Secondary accession number(s): Q0WLS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health