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Protein

Protein SPA1-RELATED 4

Gene

SPA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Repressor of photomorphogenesis in the light. Probably part of the COP1/SPA E3 ubiquitin-protein ligase complex.3 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • identical protein binding Source: IntAct
  • protein kinase activity Source: InterPro

GO - Biological processi

  • red, far-red light phototransduction Source: UniProtKB-KW
  • response to blue light Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processPhytochrome signaling pathway, Ubl conjugation pathway
LigandNucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPA1-RELATED 4
Gene namesi
Name:SPA4
Ordered Locus Names:At1g53090
ORF Names:F8L10.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G53090
TAIRilocus:2037005 AT1G53090

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced hypocotyl elongation in blue light.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003634941 – 794Protein SPA1-RELATED 4Add BLAST794

Proteomic databases

PaxDbiQ94BM7

PTM databases

iPTMnetiQ94BM7

Expressioni

Inductioni

Up-regulated by red, far-red and blue light.2 Publications

Gene expression databases

ExpressionAtlasiQ94BM7 baseline and differential
GenevisibleiQ94BM7 AT

Interactioni

Subunit structurei

Interacts with COP1 and CO (PubMed:12887588, PubMed:16854975). Binds to CRY1 in response to blue light, this interaction prevents SPA1/COP1 complex formation and thus avoid COP1-dependent degradation of the transcription factor HY5 by the proteasome and promotes hypocotyl elongation (PubMed:21511871).3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi26968, 11 interactors
IntActiQ94BM7, 4 interactors
STRINGi3702.AT1G53090.1

Structurei

3D structure databases

ProteinModelPortaliQ94BM7
SMRiQ94BM7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 268Protein kinasePROSITE-ProRule annotationAdd BLAST268
Repeati482 – 521WD 1Add BLAST40
Repeati531 – 571WD 2Add BLAST41
Repeati574 – 614WD 3Add BLAST41
Repeati616 – 656WD 4Add BLAST41
Repeati660 – 698WD 5Add BLAST39
Repeati707 – 746WD 6Add BLAST40
Repeati762 – 794WD 7Add BLAST33

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili272 – 326Sequence analysisAdd BLAST55

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi635 – 649DWD boxAdd BLAST15

Domaini

The protein kinase domain is predicted to be catalytically inactive. The DWD box is required for interaction with DDB1A (By similarity).PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1033 Eukaryota
ENOG410XS0B LUCA
HOGENOMiHOG000029775
InParanoidiQ94BM7
OMAiFECESII
OrthoDBiEOG093602MQ
PhylomeDBiQ94BM7

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 2 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q94BM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGSSESSSR GLNNTSGVSE FCTDGSKSLS HIDYVRSLLG SHKEANLGGL
60 70 80 90 100
DDDSIVRALE CEDVSLRQWL DNPDRSVDAF ECFHVFRQIV EIVNAAHSQG
110 120 130 140 150
IVVHNVRPSC FVMSSFNNVS FIESASCSDS GSDEDATTKS REIGSSRQEE
160 170 180 190 200
ILSERRSKQQ EEVKKQPFPM KQILAMEMSW YTSHEEDNGS LCNCASDIYR
210 220 230 240 250
LGVLLFELFC PVSSREEKSR TMSSLRHRVL PPQILLNWPK EASFCLWLLH
260 270 280 290 300
PEPSCRPSMS ELLQSEFINE PRENLEEREA AMELRDRIEE QELLLEFLFL
310 320 330 340 350
IQQRKQEAAD KLQDTISLLS SDIDQVVKRQ LVLQQKGRDV RSFLASRKRI
360 370 380 390 400
RQGAETTAAE EENDDNSIDE ESKLDDTLES TLLESSRLMR NLKKLESVYF
410 420 430 440 450
ATRYRQIKAA TAAEKPLARY YSALSCNGRS SEKSSMSQPS KDPINDSRQG
460 470 480 490 500
GWIDPFLEGL CKYLSFSKLR VKADLKQGDL LNSSNLVCAI GFDRDGEFFA
510 520 530 540 550
TAGVNKKIKI FECESIIKDG RDIHYPVVEL ASRSKLSGIC WNSYIKSQVA
560 570 580 590 600
SSNFEGVVQV WDVARNQLVT EMKEHEKRVW SIDYSSADPT LLASGSDDGS
610 620 630 640 650
VKLWSINQGV SIGTIKTKAN ICCVQFPSET GRSLAFGSAD HKVYYYDLRN
660 670 680 690 700
PKLPLCTMIG HHKTVSYVRF VDSSTLVSSS TDNTLKLWDL SMSISGINET
710 720 730 740 750
PLHSFMGHTN VKNFVGLSVS DGYIATGSET NEVFVYHKAF PMPVLSYKFK
760 770 780 790
TIDPVSELEV DDASQFISSV CWRGQSSTLV AANSTGNIKI LEMV
Length:794
Mass (Da):89,071
Last modified:December 1, 2001 - v1
Checksum:i5202D640029F512F
GO

Sequence cautioni

The sequence AAF87859 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022520 Genomic DNA Translation: AAF87859.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE32888.1
CP002684 Genomic DNA Translation: AEE32889.1
CP002684 Genomic DNA Translation: ANM59146.1
AY040023 mRNA Translation: AAK64180.1
AY133883 mRNA Translation: AAM91817.1
PIRiE96571
RefSeqiNP_001321533.1, NM_001333576.1
NP_175717.1, NM_104188.4
NP_849802.1, NM_179471.2
UniGeneiAt.21898

Genome annotation databases

EnsemblPlantsiAT1G53090.1; AT1G53090.1; AT1G53090
AT1G53090.2; AT1G53090.2; AT1G53090
AT1G53090.3; AT1G53090.3; AT1G53090
GeneIDi841743
GrameneiAT1G53090.1; AT1G53090.1; AT1G53090
AT1G53090.2; AT1G53090.2; AT1G53090
AT1G53090.3; AT1G53090.3; AT1G53090
KEGGiath:AT1G53090

Similar proteinsi

Entry informationi

Entry nameiSPA4_ARATH
AccessioniPrimary (citable) accession number: Q94BM7
Secondary accession number(s): Q9LNN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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