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Protein

Protein SPA1-RELATED 4

Gene

SPA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of photomorphogenesis in the light. Probably part of the COP1/SPA E3 ubiquitin-protein ligase complex.3 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • identical protein binding Source: IntAct
  • protein kinase activity Source: InterPro

GO - Biological processi

  • red, far-red light phototransduction Source: UniProtKB-KW
  • response to blue light Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phytochrome signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPA1-RELATED 4
Gene namesi
Name:SPA4
Ordered Locus Names:At1g53090
ORF Names:F8L10.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53090.

Subcellular locationi

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: TAIR
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced hypocotyl elongation in blue light.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794Protein SPA1-RELATED 4PRO_0000363494Add
BLAST

Proteomic databases

PaxDbiQ94BM7.
PRIDEiQ94BM7.

PTM databases

iPTMnetiQ94BM7.

Expressioni

Inductioni

Up-regulated by red, far-red and blue light.2 Publications

Gene expression databases

GenevisibleiQ94BM7. AT.

Interactioni

Subunit structurei

Interacts with COP1 and CO (PubMed:12887588, PubMed:16854975). Binds to CRY1 in response to blue light, this interaction prevents SPA1/COP1 complex formation and thus avoid COP1-dependent degradation of the transcription factor HY5 by the proteasome and promotes hypocotyl elongation (PubMed:21511871).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-626943,EBI-626943
COL1O500557EBI-626943,EBI-1112154
COP1P4325414EBI-626943,EBI-301649
SPA1Q9SYX29EBI-626943,EBI-626992
SPA3Q9LJR36EBI-626943,EBI-626921

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi26968. 11 interactions.
IntActiQ94BM7. 4 interactions.
STRINGi3702.AT1G53090.1.

Structurei

3D structure databases

ProteinModelPortaliQ94BM7.
SMRiQ94BM7. Positions 61-265, 476-793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 268268Protein kinasePROSITE-ProRule annotationAdd
BLAST
Repeati482 – 52140WD 1Add
BLAST
Repeati531 – 57141WD 2Add
BLAST
Repeati574 – 61441WD 3Add
BLAST
Repeati616 – 65641WD 4Add
BLAST
Repeati660 – 69839WD 5Add
BLAST
Repeati707 – 74640WD 6Add
BLAST
Repeati762 – 79433WD 7Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili272 – 32655Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi635 – 64915DWD boxAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive. The DWD box is required for interaction with DDB1A (By similarity).PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1033. Eukaryota.
ENOG410XS0B. LUCA.
HOGENOMiHOG000029775.
InParanoidiQ94BM7.
OMAiFECESII.
OrthoDBiEOG093602MQ.
PhylomeDBiQ94BM7.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94BM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGSSESSSR GLNNTSGVSE FCTDGSKSLS HIDYVRSLLG SHKEANLGGL
60 70 80 90 100
DDDSIVRALE CEDVSLRQWL DNPDRSVDAF ECFHVFRQIV EIVNAAHSQG
110 120 130 140 150
IVVHNVRPSC FVMSSFNNVS FIESASCSDS GSDEDATTKS REIGSSRQEE
160 170 180 190 200
ILSERRSKQQ EEVKKQPFPM KQILAMEMSW YTSHEEDNGS LCNCASDIYR
210 220 230 240 250
LGVLLFELFC PVSSREEKSR TMSSLRHRVL PPQILLNWPK EASFCLWLLH
260 270 280 290 300
PEPSCRPSMS ELLQSEFINE PRENLEEREA AMELRDRIEE QELLLEFLFL
310 320 330 340 350
IQQRKQEAAD KLQDTISLLS SDIDQVVKRQ LVLQQKGRDV RSFLASRKRI
360 370 380 390 400
RQGAETTAAE EENDDNSIDE ESKLDDTLES TLLESSRLMR NLKKLESVYF
410 420 430 440 450
ATRYRQIKAA TAAEKPLARY YSALSCNGRS SEKSSMSQPS KDPINDSRQG
460 470 480 490 500
GWIDPFLEGL CKYLSFSKLR VKADLKQGDL LNSSNLVCAI GFDRDGEFFA
510 520 530 540 550
TAGVNKKIKI FECESIIKDG RDIHYPVVEL ASRSKLSGIC WNSYIKSQVA
560 570 580 590 600
SSNFEGVVQV WDVARNQLVT EMKEHEKRVW SIDYSSADPT LLASGSDDGS
610 620 630 640 650
VKLWSINQGV SIGTIKTKAN ICCVQFPSET GRSLAFGSAD HKVYYYDLRN
660 670 680 690 700
PKLPLCTMIG HHKTVSYVRF VDSSTLVSSS TDNTLKLWDL SMSISGINET
710 720 730 740 750
PLHSFMGHTN VKNFVGLSVS DGYIATGSET NEVFVYHKAF PMPVLSYKFK
760 770 780 790
TIDPVSELEV DDASQFISSV CWRGQSSTLV AANSTGNIKI LEMV
Length:794
Mass (Da):89,071
Last modified:December 1, 2001 - v1
Checksum:i5202D640029F512F
GO

Sequence cautioni

The sequence AAF87859 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022520 Genomic DNA. Translation: AAF87859.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32888.1.
CP002684 Genomic DNA. Translation: AEE32889.1.
AY040023 mRNA. Translation: AAK64180.1.
AY133883 mRNA. Translation: AAM91817.1.
PIRiE96571.
RefSeqiNP_175717.1. NM_104188.3.
NP_849802.1. NM_179471.1.
UniGeneiAt.21898.

Genome annotation databases

EnsemblPlantsiAT1G53090.1; AT1G53090.1; AT1G53090.
AT1G53090.2; AT1G53090.2; AT1G53090.
GeneIDi841743.
GrameneiAT1G53090.1; AT1G53090.1; AT1G53090.
AT1G53090.2; AT1G53090.2; AT1G53090.
KEGGiath:AT1G53090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022520 Genomic DNA. Translation: AAF87859.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32888.1.
CP002684 Genomic DNA. Translation: AEE32889.1.
AY040023 mRNA. Translation: AAK64180.1.
AY133883 mRNA. Translation: AAM91817.1.
PIRiE96571.
RefSeqiNP_175717.1. NM_104188.3.
NP_849802.1. NM_179471.1.
UniGeneiAt.21898.

3D structure databases

ProteinModelPortaliQ94BM7.
SMRiQ94BM7. Positions 61-265, 476-793.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26968. 11 interactions.
IntActiQ94BM7. 4 interactions.
STRINGi3702.AT1G53090.1.

PTM databases

iPTMnetiQ94BM7.

Proteomic databases

PaxDbiQ94BM7.
PRIDEiQ94BM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53090.1; AT1G53090.1; AT1G53090.
AT1G53090.2; AT1G53090.2; AT1G53090.
GeneIDi841743.
GrameneiAT1G53090.1; AT1G53090.1; AT1G53090.
AT1G53090.2; AT1G53090.2; AT1G53090.
KEGGiath:AT1G53090.

Organism-specific databases

TAIRiAT1G53090.

Phylogenomic databases

eggNOGiKOG1033. Eukaryota.
ENOG410XS0B. LUCA.
HOGENOMiHOG000029775.
InParanoidiQ94BM7.
OMAiFECESII.
OrthoDBiEOG093602MQ.
PhylomeDBiQ94BM7.

Enzyme and pathway databases

ReactomeiR-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.

Miscellaneous databases

PROiQ94BM7.

Gene expression databases

GenevisibleiQ94BM7. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA4_ARATH
AccessioniPrimary (citable) accession number: Q94BM7
Secondary accession number(s): Q9LNN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.