Reviewed, UniProtKB/Swiss-Prot Q94B74 (NUDT2_ARATH)

Names and origin

Protein names

Recommended name:
    Nudix hydrolase 2
      Short name=AtNUDT2
    EC=3.6.1.-
Alternative name(s):
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
    NADH pyrophosphatase
    EC=3.6.1.22

Gene names

Name:	NUDT2
Synonyms:	NUDX2
Ordered Locus Names:	At5g47650
ORF Names:	MNJ7.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably mediates the hydrolysis of some nucleoside diphosphate derivatives. In vitro, it can use both NADH and ADP-ribose as substrates; however the relevance of such substrates in vivo is unclear. Ref.3
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. NAD+ + H2O = AMP + NMN.
Cofactor	Magnesium or manganese By similarity.
Tissue specificity	Expressed in roots, stems and leaves. Ref.3
Induction	By paraquat, drought and high salinity.
Miscellaneous	Overexpression of NUTD2 confers enhanced tolerance to oxidative stress.
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.
Biophysicochemical properties	Kinetic parameters: KM=16.9 µM for ADP-ribose KM=23.1 µM for NADH Vmax=0.20 µmol/min/mg enzyme with ADP-ribose as substrate Vmax=0.16 µmol/min/mg enzyme with NADH as substrate

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to oxidative stress Ref.4 Inferred from mutant phenotype. Source: TAIR
Molecular function	ADP-ribose diphosphatase activity Ref.3 Inferred from direct assay. Source: TAIR NAD or NADH binding Ref.3 Inferred from direct assay. Source: TAIR NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Nudix hydrolase 2		PRO_0000057122
Regions
	Domain	111 – 240	130	Nudix hydrolase
	Motif	147 – 168	22	Nudix box
Sites
	Metal binding	162	1	Magnesium or manganese By similarity
	Metal binding	166	1	Magnesium or manganese By similarity
Experimental info
	Sequence conflict	125	1	E → EK in BAB09091. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q94B74-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C0907A87DD5D2A7D	FASTA	278	31,611
	10 20 30 40 50 60 MSASSSSTNP MSREDATTLL PSVQDKYGGV MTEMTHPMDP SLFSTLLRSS LSTWTLQGKK 70 80 90 100 110 120 GVWIKLPKQL IGLAETAVKE GFWFHHAEKD YLMLVYWIPK EDDTLPANAS HRVGIGAFVI 130 140 150 160 170 180 NHNKEVLVVQ EKTGRFQGQG IWKFPTGVVN EGEDIHDGSV REVKEETGVD TEFDQILAFR 190 200 210 220 230 240 QTHKAFFGKS DLFFVCMLKP LSLEINAQES EIEAAQWMPW EEYINQPFVQ NYELLRYMTD 250 260 270 ICSAKTNGDY EGFTPLRVSA PDQQGNLYYN TRDLHSRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Comprehensive analysis of cytosolic nudix hydrolases in Arabidopsis thaliana." Ogawa T., Ueda Y., Yoshimura K., Shigeoka S. J. Biol. Chem. 280:25277-25283(2005) [PubMed: 15878881] [Abstract] Cited for: FUNCTION IN VITRO, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[4]	"Overexpression of an ADP-ribose pyrophosphatase, AtNUDX2, confers enhanced tolerance to oxidative stress in Arabidopsis plants." Ogawa T., Ishikawa K., Harada K., Fukusaki E., Yoshimura K., Shigeoka S. Plant J. 57:289-301(2009) [PubMed: 18798872] [Abstract] Cited for: FUNCTION, INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB025628 Genomic DNA. Translation: BAB09091.1. Different initiation. AY042806 mRNA. Translation: AAK68746.1. AY064646 mRNA. Translation: AAL47357.1.
IPI	IPI00532396.
RefSeq	NP_568687.1.
UniGene	At.71142
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q94B74.
Genome annotation databases
GeneID	834816.
GenomeReviews	Gene locus AT5G47650 in contig BA000015_GR.
KEGG	ath:AT5G47650.
NMPDR	fig|3702.1.peg.26577.
Organism-specific databases
TAIR	At5g47650.
Phylogenomic databases
OMA	Q94B74. FVQKHEL.
Enzyme and pathway databases
BRENDA	3.6.1.13. 302. 3.6.1.22. 302.
Gene expression databases
GermOnline	AT5G47650. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003293. GFG_antis. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01356. GFGPROTEIN.
PROSITE	PS00893. NUDIX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDT2_ARATH

Accession

Primary (citable) accession number: Q94B74
Secondary accession number(s): Q9FGJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	December 1, 2001
Last modified:	July 7, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)