Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic

Gene

ISPH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is essential for chloroplast development.2 Publications

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.

Cofactori

[3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster per subunit.By similarity

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic (DXR)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (ISPE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin), chloroplastic (ISPG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (3Fe-4S)By similarity1
Binding sitei152SubstrateBy similarity1
Metal bindingi213Iron-sulfur (3Fe-4S)By similarity1
Binding sitei241SubstrateBy similarity1
Binding sitei312SubstrateBy similarity1
Metal bindingi350Iron-sulfur (3Fe-4S)By similarity1
Binding sitei441SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT4G34350-MONOMER-9581.
MetaCyc:AT4G34350-MONOMER-9581.
UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (EC:1.17.7.4)
Alternative name(s):
Protein CHLOROPLAST BIOGENESIS 6
Gene namesi
Name:ISPH
Synonyms:CLB6, HDR
Ordered Locus Names:At4g34350
ORF Names:F10M10.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34350.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38ChloroplastSequence analysisAdd BLAST38
ChainiPRO_000041759239 – 4664-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplasticAdd BLAST428

Proteomic databases

PaxDbiQ94B35.
PRIDEiQ94B35.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

Circadian-regulated with a peak in the late period of dark phase and early period of the light phase.1 Publication

Gene expression databases

GenevisibleiQ94B35. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G34350.1.

Structurei

3D structure databases

ProteinModelPortaliQ94B35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni379 – 381Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the IspH family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIDH. Eukaryota.
COG0761. LUCA.
HOGENOMiHOG000240388.
InParanoidiQ94B35.
KOiK03527.
OMAiAYETRRH.
OrthoDBiEOG093608MF.
PhylomeDBiQ94B35.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94B35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVALQFSRL CVRPDTFVRE NHLSGSGSLR RRKALSVRCS SGDENAPSPS
60 70 80 90 100
VVMDSDFDAK VFRKNLTRSD NYNRKGFGHK EETLKLMNRE YTSDILETLK
110 120 130 140 150
TNGYTYSWGD VTVKLAKAYG FCWGVERAVQ IAYEARKQFP EERLWITNEI
160 170 180 190 200
IHNPTVNKRL EDMDVKIIPV EDSKKQFDVV EKDDVVILPA FGAGVDEMYV
210 220 230 240 250
LNDKKVQIVD TTCPWVTKVW NTVEKHKKGE YTSVIHGKYN HEETIATASF
260 270 280 290 300
AGKYIIVKNM KEANYVCDYI LGGQYDGSSS TKEEFMEKFK YAISKGFDPD
310 320 330 340 350
NDLVKVGIAN QTTMLKGETE EIGRLLETTM MRKYGVENVS GHFISFNTIC
360 370 380 390 400
DATQERQDAI YELVEEKIDL MLVVGGWNSS NTSHLQEISE ARGIPSYWID
410 420 430 440 450
SEKRIGPGNK IAYKLHYGEL VEKENFLPKG PITIGVTSGA STPDKVVEDA
460
LVKVFDIKRE ELLQLA
Length:466
Mass (Da):52,781
Last modified:December 1, 2001 - v1
Checksum:iB008808517890DC6
GO

Sequence cautioni

The sequence AAW82381 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD94833 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB36712 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80152 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY883838 Genomic DNA. Translation: AAW82381.1. Sequence problems.
AY168881 mRNA. Translation: AAN87171.1.
AL035521 Genomic DNA. Translation: CAB36712.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80152.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86362.1.
AY042877 mRNA. Translation: AAK68817.1.
AY081454 mRNA. Translation: AAM10016.1.
AK317707 mRNA. Translation: BAH20366.1.
AK222055 mRNA. Translation: BAD94833.1. Different initiation.
PIRiT04781.
RefSeqiNP_567965.1. NM_119600.4.
UniGeneiAt.2618.

Genome annotation databases

EnsemblPlantsiAT4G34350.1; AT4G34350.1; AT4G34350.
GeneIDi829585.
GrameneiAT4G34350.1; AT4G34350.1; AT4G34350.
KEGGiath:AT4G34350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY883838 Genomic DNA. Translation: AAW82381.1. Sequence problems.
AY168881 mRNA. Translation: AAN87171.1.
AL035521 Genomic DNA. Translation: CAB36712.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80152.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86362.1.
AY042877 mRNA. Translation: AAK68817.1.
AY081454 mRNA. Translation: AAM10016.1.
AK317707 mRNA. Translation: BAH20366.1.
AK222055 mRNA. Translation: BAD94833.1. Different initiation.
PIRiT04781.
RefSeqiNP_567965.1. NM_119600.4.
UniGeneiAt.2618.

3D structure databases

ProteinModelPortaliQ94B35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G34350.1.

Proteomic databases

PaxDbiQ94B35.
PRIDEiQ94B35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34350.1; AT4G34350.1; AT4G34350.
GeneIDi829585.
GrameneiAT4G34350.1; AT4G34350.1; AT4G34350.
KEGGiath:AT4G34350.

Organism-specific databases

TAIRiAT4G34350.

Phylogenomic databases

eggNOGiENOG410IIDH. Eukaryota.
COG0761. LUCA.
HOGENOMiHOG000240388.
InParanoidiQ94B35.
KOiK03527.
OMAiAYETRRH.
OrthoDBiEOG093608MF.
PhylomeDBiQ94B35.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.
BioCyciARA:AT4G34350-MONOMER-9581.
MetaCyc:AT4G34350-MONOMER-9581.

Miscellaneous databases

PROiQ94B35.

Gene expression databases

GenevisibleiQ94B35. AT.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiIPR003451. LytB/IspH.
[Graphical view]
PfamiPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPH_ARATH
AccessioniPrimary (citable) accession number: Q94B35
Secondary accession number(s): B9DHZ9
, Q56WI5, Q5EGH0, Q9SZ00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.