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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic

Gene

ISPH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is essential for chloroplast development.2 Publications

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.

Cofactori

[3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster per subunit.By similarity

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic (DXR)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (ISPD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic (ISPE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, chloroplastic (ISPF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin), chloroplastic (ISPG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (ISPH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (3Fe-4S)By similarity1
Binding sitei152SubstrateBy similarity1
Metal bindingi213Iron-sulfur (3Fe-4S)By similarity1
Binding sitei241SubstrateBy similarity1
Binding sitei312SubstrateBy similarity1
Metal bindingi350Iron-sulfur (3Fe-4S)By similarity1
Binding sitei441SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand3Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT4G34350-MONOMER-9581
MetaCyc:AT4G34350-MONOMER-9581
UniPathwayiUPA00056; UER00097
UPA00059; UER00105

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic (EC:1.17.7.4)
Alternative name(s):
Protein CHLOROPLAST BIOGENESIS 6
Gene namesi
Name:ISPH
Synonyms:CLB6, HDR
Ordered Locus Names:At4g34350
ORF Names:F10M10.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G34350
TAIRilocus:2116164 AT4G34350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Albino phenotype and seedling lethal when homozygous. The phenotype is caused by an early arrest in chloroplast differentiation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38ChloroplastSequence analysisAdd BLAST38
ChainiPRO_000041759239 – 4664-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplasticAdd BLAST428

Proteomic databases

PaxDbiQ94B35
PRIDEiQ94B35

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

Circadian-regulated with a peak in the late period of dark phase and early period of the light phase.1 Publication

Gene expression databases

ExpressionAtlasiQ94B35 baseline and differential
GenevisibleiQ94B35 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G34350.1

Structurei

3D structure databases

ProteinModelPortaliQ94B35
SMRiQ94B35
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni379 – 381Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the IspH family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIDH Eukaryota
COG0761 LUCA
HOGENOMiHOG000240388
InParanoidiQ94B35
KOiK03527
OMAiQSASFIV
OrthoDBiEOG093608MF
PhylomeDBiQ94B35

Family and domain databases

CDDicd13944 lytB_ispH, 1 hit
HAMAPiMF_00191 IspH, 1 hit
InterProiView protein in InterPro
IPR003451 LytB/IspH
PfamiView protein in Pfam
PF02401 LYTB, 1 hit
TIGRFAMsiTIGR00216 ispH_lytB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94B35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVALQFSRL CVRPDTFVRE NHLSGSGSLR RRKALSVRCS SGDENAPSPS
60 70 80 90 100
VVMDSDFDAK VFRKNLTRSD NYNRKGFGHK EETLKLMNRE YTSDILETLK
110 120 130 140 150
TNGYTYSWGD VTVKLAKAYG FCWGVERAVQ IAYEARKQFP EERLWITNEI
160 170 180 190 200
IHNPTVNKRL EDMDVKIIPV EDSKKQFDVV EKDDVVILPA FGAGVDEMYV
210 220 230 240 250
LNDKKVQIVD TTCPWVTKVW NTVEKHKKGE YTSVIHGKYN HEETIATASF
260 270 280 290 300
AGKYIIVKNM KEANYVCDYI LGGQYDGSSS TKEEFMEKFK YAISKGFDPD
310 320 330 340 350
NDLVKVGIAN QTTMLKGETE EIGRLLETTM MRKYGVENVS GHFISFNTIC
360 370 380 390 400
DATQERQDAI YELVEEKIDL MLVVGGWNSS NTSHLQEISE ARGIPSYWID
410 420 430 440 450
SEKRIGPGNK IAYKLHYGEL VEKENFLPKG PITIGVTSGA STPDKVVEDA
460
LVKVFDIKRE ELLQLA
Length:466
Mass (Da):52,781
Last modified:December 1, 2001 - v1
Checksum:iB008808517890DC6
GO

Sequence cautioni

The sequence AAW82381 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD94833 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB36712 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80152 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY883838 Genomic DNA Translation: AAW82381.1 Sequence problems.
AY168881 mRNA Translation: AAN87171.1
AL035521 Genomic DNA Translation: CAB36712.1 Sequence problems.
AL161585 Genomic DNA Translation: CAB80152.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86362.1
AY042877 mRNA Translation: AAK68817.1
AY081454 mRNA Translation: AAM10016.1
AK317707 mRNA Translation: BAH20366.1
AK222055 mRNA Translation: BAD94833.1 Different initiation.
PIRiT04781
RefSeqiNP_567965.1, NM_119600.4
UniGeneiAt.2618

Genome annotation databases

EnsemblPlantsiAT4G34350.1; AT4G34350.1; AT4G34350
GeneIDi829585
GrameneiAT4G34350.1; AT4G34350.1; AT4G34350
KEGGiath:AT4G34350

Similar proteinsi

Entry informationi

Entry nameiISPH_ARATH
AccessioniPrimary (citable) accession number: Q94B35
Secondary accession number(s): B9DHZ9
, Q56WI5, Q5EGH0, Q9SZ00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health