Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-aspartate oxidase, chloroplastic

Gene

AO

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of L-aspartate to iminoaspartate. Can complement nadB-deficient E.coli mutant. Plays a role in stomatal immunity.2 Publications

Catalytic activityi

L-aspartate + O2 = iminosuccinate + H2O2.

Cofactori

FADBy similarity

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes iminoaspartate from L-aspartate (oxidase route).
Proteins known to be involved in this subpathway in this organism are:
  1. L-aspartate oxidase, chloroplastic (AO)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes iminoaspartate from L-aspartate (oxidase route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei313 – 3131By similarity
Active sitei341 – 3411By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 10215FADSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • NAD biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G14760-MONOMER.
UniPathwayiUPA00253; UER00326.

Names & Taxonomyi

Protein namesi
Recommended name:
L-aspartate oxidase, chloroplastic (EC:1.4.3.16)
Alternative name(s):
Protein FLAGELLIN-INSENSITIVE 4
Gene namesi
Name:AO
Synonyms:FIN4
Ordered Locus Names:At5g14760
ORF Names:T9L3.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G14760.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474ChloroplastSequence analysisAdd
BLAST
Chaini75 – 651577L-aspartate oxidase, chloroplasticPRO_0000423478Add
BLAST

Proteomic databases

PaxDbiQ94AY1.
PRIDEiQ94AY1.

Expressioni

Gene expression databases

GenevisibleiQ94AY1. AT.

Interactioni

Subunit structurei

Interacts in vitro with QS (PubMed:18978034).1 Publication

Protein-protein interaction databases

BioGridi16605. 37 interactions.
IntActiQ94AY1. 4 interactions.
STRINGi3702.AT5G14760.1.

Structurei

3D structure databases

ProteinModelPortaliQ94AY1.
SMRiQ94AY1. Positions 81-642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IQ7H. Eukaryota.
COG0029. LUCA.
HOGENOMiHOG000160476.
InParanoidiQ94AY1.
KOiK00278.
OMAiRRIVHVR.
OrthoDBiEOG093604JS.
PhylomeDBiQ94AY1.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR005288. NadB.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR00551. nadB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94AY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHVSTGNI HNFYLAGQVY RGQAFSWSSA STFMANPFKE PSWSSGVFKA
60 70 80 90 100
LKAERCGCYS RGISPISETS KPIRAVSVSS STKYYDFTVI GSGVAGLRYA
110 120 130 140 150
LEVAKQGTVA VITKDEPHES NTNYAQGGVS AVLCPLDSVE SHMRDTMVAG
160 170 180 190 200
AHLCDEETVR VVCTEGPERI RELIAMGASF DHGEDGNLHL AREGGHSHCR
210 220 230 240 250
IVHAADMTGR EIERALLEAV LNDPNISVFK HHFAIDLLTS QDGLNTVCHG
260 270 280 290 300
VDTLNIKTNE VVRFISKVTL LASGGAGHIY PSTTNPLVAT GDGMAMAHRA
310 320 330 340 350
QAVISNMEFV QFHPTALADE GLPIKLQTAR ENAFLITEAV RGDGGILYNL
360 370 380 390 400
GMERFMPVYD ERAELAPRDV VARSIDDQLK KRNEKYVLLD ISHKPREKIL
410 420 430 440 450
AHFPNIASEC LKHGLDITRQ PIPVVPAAHY MCGGVRAGLQ GETNVLGLFV
460 470 480 490 500
AGEVACTGLH GANRLASNSL LEALVFARRA VQPSTELMKR TRLDVCASEK
510 520 530 540 550
WTRPVVATAR LLGDEVIAKI IALTKEVRRE LQEVMWKYVG IVRSTIRLTT
560 570 580 590 600
AERKIAELEA KWETFLFEHG WEQTVVALEA CEMRNLFCCA KLVVSSALAR
610 620 630 640 650
HESRGLHYMT DFPFVEESKR IPTIILPSSP TTASWSSRRL QNISSSSLID

C
Length:651
Mass (Da):71,412
Last modified:December 1, 2001 - v1
Checksum:i0CE18268085DB738
GO

Sequence cautioni

The sequence CAC01875 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391149 Genomic DNA. Translation: CAC01875.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92074.1.
AY045626 mRNA. Translation: AAK73984.1.
BT001009 mRNA. Translation: AAN46763.1.
PIRiT51421.
RefSeqiNP_568304.1. NM_121480.3.
UniGeneiAt.9942.

Genome annotation databases

EnsemblPlantsiAT5G14760.1; AT5G14760.1; AT5G14760.
GeneIDi831328.
GrameneiAT5G14760.1; AT5G14760.1; AT5G14760.
KEGGiath:AT5G14760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391149 Genomic DNA. Translation: CAC01875.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92074.1.
AY045626 mRNA. Translation: AAK73984.1.
BT001009 mRNA. Translation: AAN46763.1.
PIRiT51421.
RefSeqiNP_568304.1. NM_121480.3.
UniGeneiAt.9942.

3D structure databases

ProteinModelPortaliQ94AY1.
SMRiQ94AY1. Positions 81-642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16605. 37 interactions.
IntActiQ94AY1. 4 interactions.
STRINGi3702.AT5G14760.1.

Proteomic databases

PaxDbiQ94AY1.
PRIDEiQ94AY1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G14760.1; AT5G14760.1; AT5G14760.
GeneIDi831328.
GrameneiAT5G14760.1; AT5G14760.1; AT5G14760.
KEGGiath:AT5G14760.

Organism-specific databases

TAIRiAT5G14760.

Phylogenomic databases

eggNOGiENOG410IQ7H. Eukaryota.
COG0029. LUCA.
HOGENOMiHOG000160476.
InParanoidiQ94AY1.
KOiK00278.
OMAiRRIVHVR.
OrthoDBiEOG093604JS.
PhylomeDBiQ94AY1.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00326.
BioCyciARA:AT5G14760-MONOMER.

Miscellaneous databases

PROiQ94AY1.

Gene expression databases

GenevisibleiQ94AY1. AT.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR005288. NadB.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR00551. nadB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADB_ARATH
AccessioniPrimary (citable) accession number: Q94AY1
Secondary accession number(s): Q9LER1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The viable mutant fin4 (T-DNA insertion in the promoter) is impaired in the RBOHD-dependent pathogen-associated molecular pattern (PAMP)-induced reactive oxygen species (ROS) burst and stomatal closure.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.