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Protein

L-aspartate oxidase, chloroplastic

Gene

AO

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of L-aspartate to iminoaspartate. Can complement nadB-deficient E.coli mutant. Plays a role in stomatal immunity.2 Publications

Miscellaneous

The viable mutant fin4 (T-DNA insertion in the promoter) is impaired in the RBOHD-dependent pathogen-associated molecular pattern (PAMP)-induced reactive oxygen species (ROS) burst and stomatal closure.1 Publication

Catalytic activityi

L-aspartate + O2 = iminosuccinate + H2O2.

Cofactori

FADBy similarity

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes iminoaspartate from L-aspartate (oxidase route).
Proteins known to be involved in this subpathway in this organism are:
  1. L-aspartate oxidase, chloroplastic (AO), L-aspartate oxidase (AXX17_At5g14260)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes iminoaspartate from L-aspartate (oxidase route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei313By similarity1
Active sitei341By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 102FADSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

  • NAD biosynthetic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processPyridine nucleotide biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G14760-MONOMER
UniPathwayiUPA00253; UER00326

Names & Taxonomyi

Protein namesi
Recommended name:
L-aspartate oxidase, chloroplastic (EC:1.4.3.16)
Alternative name(s):
Protein FLAGELLIN-INSENSITIVE 4
Gene namesi
Name:AO
Synonyms:FIN4
Ordered Locus Names:At5g14760
ORF Names:T9L3.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G14760
TAIRilocus:2185480 AT5G14760

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 74ChloroplastSequence analysisAdd BLAST74
ChainiPRO_000042347875 – 651L-aspartate oxidase, chloroplasticAdd BLAST577

Proteomic databases

PaxDbiQ94AY1

Expressioni

Gene expression databases

ExpressionAtlasiQ94AY1 baseline and differential
GenevisibleiQ94AY1 AT

Interactioni

Subunit structurei

Interacts in vitro with QS (PubMed:18978034).1 Publication

Protein-protein interaction databases

BioGridi16605, 37 interactors
IntActiQ94AY1, 4 interactors
STRINGi3702.AT5G14760.1

Structurei

3D structure databases

ProteinModelPortaliQ94AY1
SMRiQ94AY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IQ7H Eukaryota
COG0029 LUCA
HOGENOMiHOG000160476
InParanoidiQ94AY1
KOiK00278
OMAiHCVQWLI
OrthoDBiEOG093604JS
PhylomeDBiQ94AY1

Family and domain databases

Gene3Di3.50.50.60, 2 hits
3.90.700.10, 1 hit
InterProiView protein in InterPro
IPR003953 FAD-binding_2
IPR036188 FAD/NAD-bd_sf
IPR037099 Fum_R/Succ_DH_flav-like_C_sf
IPR015939 Fum_Rdtase/Succ_DH_flav-like_C
IPR005288 NadB
IPR027477 Succ_DH/fumarate_Rdtase_cat_sf
PANTHERiPTHR42716:SF2 PTHR42716:SF2, 1 hit
PfamiView protein in Pfam
PF00890 FAD_binding_2, 1 hit
PF02910 Succ_DH_flav_C, 1 hit
SUPFAMiSSF46977 SSF46977, 1 hit
SSF51905 SSF51905, 2 hits
SSF56425 SSF56425, 1 hit
TIGRFAMsiTIGR00551 nadB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94AY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHVSTGNI HNFYLAGQVY RGQAFSWSSA STFMANPFKE PSWSSGVFKA
60 70 80 90 100
LKAERCGCYS RGISPISETS KPIRAVSVSS STKYYDFTVI GSGVAGLRYA
110 120 130 140 150
LEVAKQGTVA VITKDEPHES NTNYAQGGVS AVLCPLDSVE SHMRDTMVAG
160 170 180 190 200
AHLCDEETVR VVCTEGPERI RELIAMGASF DHGEDGNLHL AREGGHSHCR
210 220 230 240 250
IVHAADMTGR EIERALLEAV LNDPNISVFK HHFAIDLLTS QDGLNTVCHG
260 270 280 290 300
VDTLNIKTNE VVRFISKVTL LASGGAGHIY PSTTNPLVAT GDGMAMAHRA
310 320 330 340 350
QAVISNMEFV QFHPTALADE GLPIKLQTAR ENAFLITEAV RGDGGILYNL
360 370 380 390 400
GMERFMPVYD ERAELAPRDV VARSIDDQLK KRNEKYVLLD ISHKPREKIL
410 420 430 440 450
AHFPNIASEC LKHGLDITRQ PIPVVPAAHY MCGGVRAGLQ GETNVLGLFV
460 470 480 490 500
AGEVACTGLH GANRLASNSL LEALVFARRA VQPSTELMKR TRLDVCASEK
510 520 530 540 550
WTRPVVATAR LLGDEVIAKI IALTKEVRRE LQEVMWKYVG IVRSTIRLTT
560 570 580 590 600
AERKIAELEA KWETFLFEHG WEQTVVALEA CEMRNLFCCA KLVVSSALAR
610 620 630 640 650
HESRGLHYMT DFPFVEESKR IPTIILPSSP TTASWSSRRL QNISSSSLID

C
Length:651
Mass (Da):71,412
Last modified:December 1, 2001 - v1
Checksum:i0CE18268085DB738
GO

Sequence cautioni

The sequence CAC01875 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391149 Genomic DNA Translation: CAC01875.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92074.1
AY045626 mRNA Translation: AAK73984.1
BT001009 mRNA Translation: AAN46763.1
PIRiT51421
RefSeqiNP_568304.1, NM_121480.4
UniGeneiAt.9942

Genome annotation databases

EnsemblPlantsiAT5G14760.1; AT5G14760.1; AT5G14760
GeneIDi831328
GrameneiAT5G14760.1; AT5G14760.1; AT5G14760
KEGGiath:AT5G14760

Similar proteinsi

Entry informationi

Entry nameiNADB_ARATH
AccessioniPrimary (citable) accession number: Q94AY1
Secondary accession number(s): Q9LER1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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