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Protein

Chaperone protein dnaJ 3

Gene

ATJ3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Have a continuous role in plant development probably in the structural organization of compartments.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi148Zinc 1By similarity1
Metal bindingi151Zinc 1By similarity1
Metal bindingi164Zinc 2By similarity1
Metal bindingi167Zinc 2By similarity1
Metal bindingi191Zinc 2By similarity1
Metal bindingi194Zinc 2By similarity1
Metal bindingi207Zinc 1By similarity1
Metal bindingi210Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri135 – 219CR-typeAdd BLAST85

GO - Molecular functioni

GO - Biological processi

  • photoperiodism, flowering Source: TAIR
  • positive regulation of flower development Source: TAIR
  • protein folding Source: InterPro
  • regulation of ATPase activity Source: TAIR
  • response to heat Source: InterPro
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionChaperone
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-3371497. HSP90 chaperone cycle for steroid hormone receptors (SHR).

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein dnaJ 3
Short name:
AtDjA3
Short name:
AtJ3
Gene namesi
Name:ATJ3
Synonyms:A3, J3
Ordered Locus Names:At3g44110
ORF Names:F26G5.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G44110.
TAIRilocus:2081378. AT3G44110.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710791 – 417Chaperone protein dnaJ 3Add BLAST417
PropeptideiPRO_0000396765418 – 420Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei417Cysteine methyl esterBy similarity1
Lipidationi417S-farnesyl cysteineBy similarity1

Post-translational modificationi

Farnesylated.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiQ94AW8.
PRIDEiQ94AW8.

PTM databases

iPTMnetiQ94AW8.

Expressioni

Tissue specificityi

Roots, shoots, flowers, siliques and cotyledons.1 Publication

Inductioni

A heat shock at 35 degrees Celsius for 5 hours resulted in a 5-fold increase in levels.1 Publication

Gene expression databases

ExpressionAtlasiQ94AW8. baseline and differential.
GenevisibleiQ94AW8. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi8851. 4 interactors.
IntActiQ94AW8. 1 interactor.
STRINGi3702.AT3G44110.1.

Structurei

3D structure databases

ProteinModelPortaliQ94AW8.
SMRiQ94AW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 75JAdd BLAST62
Repeati148 – 155CXXCXGXG motif8
Repeati164 – 171CXXCXGXG motif8
Repeati191 – 198CXXCXGXG motif8
Repeati207 – 214CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi82 – 89Poly-Gly8
Compositional biasi400 – 407Poly-Asp8

Sequence similaritiesi

Belongs to the DnaJ family. A/I subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri135 – 219CR-typeAdd BLAST85

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
HOGENOMiHOG000226718.
InParanoidiQ94AW8.
KOiK09503.
OMAiCDGHGMK.
OrthoDBiEOG09360EQU.
PhylomeDBiQ94AW8.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q94AW8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGRGPSKKS DNTKFYEILG VPKSASPEDL KKAYKKAAIK NHPDKGGDPE
60 70 80 90 100
KFKELAQAYE VLSDPEKREI YDQYGEDALK EGMGGGGGGH DPFDIFSSFF
110 120 130 140 150
GGGPFGGNTS RQRRQRRGED VVHPLKVSLE DVYLGTMKKL SLSRNALCSK
160 170 180 190 200
CNGKGSKSGA SLKCGGCQGS GMKVSIRQLG PGMIQQMQHA CNECKGTGET
210 220 230 240 250
INDRDRCPQC KGDKVIPEKK VLEVNVEKGM QHSQKITFEG QADEAPDTVT
260 270 280 290 300
GDIVFVLQQK EHPKFKRKGE DLFVEHTLSL TEALCGFQFV LTHLDGRSLL
310 320 330 340 350
IKSNPGEVVK PDSYKAISDE GMPIYQRPFM KGKLYIHFTV EFPDSLSPDQ
360 370 380 390 400
TKALEAVLPK PSTAQLSDME IDECEETTLH DVNIEDEMRR KAQAQREAYD
410 420
DDDEDDDHPG GAQRVQCAQQ
Length:420
Mass (Da):46,444
Last modified:May 10, 2005 - v2
Checksum:i3F213CF7B2E43E04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti227E → K in AAK74013 (PubMed:14593172).Curated1
Sequence conflicti264K → Q in AAB49030 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22340 mRNA. Translation: AAB49030.1.
AF032883 Genomic DNA. Translation: AAB86892.1.
AL353814 Genomic DNA. Translation: CAB88419.1.
CP002686 Genomic DNA. Translation: AEE77863.1.
AY035087 mRNA. Translation: AAK59592.1.
AY045655 mRNA. Translation: AAK74013.1.
AY113878 mRNA. Translation: AAM44926.1.
AY088078 mRNA. Translation: AAM65624.1.
PIRiS71199.
T49127.
RefSeqiNP_189997.1. NM_114279.4. [Q94AW8-1]
UniGeneiAt.20860.
At.24417.

Genome annotation databases

EnsemblPlantsiAT3G44110.1; AT3G44110.1; AT3G44110. [Q94AW8-1]
GeneIDi823531.
GrameneiAT3G44110.1; AT3G44110.1; AT3G44110.
KEGGiath:AT3G44110.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDNAJ3_ARATH
AccessioniPrimary (citable) accession number: Q94AW8
Secondary accession number(s): O22663, Q42530
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: August 30, 2017
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families