Q94AV5 (WIP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WPP domain-interacting protein 3

Gene names

Name:	WIP3
Ordered Locus Names:	At3g13360
ORF Names:	MDC11.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates and enhances the nuclear envelope docking of RANGAP proteins mediated by WIT1 and WIT2 in the undifferentiated cells of root tips. Ref.4
Subunit structure	Component of Ran complexes at least composed of WIT1 or WIT2, RANGAP1 or RANGAP2, and WIP1 or WIP2 or WIP3 By similarity. Interacts with RANGAP1, WPP1/MAF1, and WPP2/MAF2. Ref.4
Subcellular location	Nucleus envelope. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side Ref.4.
Tissue specificity	Expressed in seedlings, roots, stems, leaves, and flowers. Ref.4
Developmental stage	First observed in roots and cotyledons of young developing seedlings. Later confined to root tips and cauline leaves tips. In flowers, detected in the stamens and at the senescence region of developing siliques. Ref.4
Sequence caution	The sequence BAB02805.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane Nucleus
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleus organization Inferred from genetic interaction PubMed 22270916. Source: TAIR
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay Ref.4. Source: TAIR nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	WPP domain-interacting protein 3		PRO_0000347198
Regions
	Transmembrane	427 – 447	21	Helical; Potential
	Coiled coil	331 – 375	45	Potential
	Motif	60 – 61	2	Nuclear localization signal 1 By similarity
	Motif	63 – 64	2	Nuclear localization signal 2 By similarity
	Motif	86 – 87	2	Nuclear localization signal 3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q94AV5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 6FAD2C63953AAC07	FASTA	459	50,274
	10 20 30 40 50 60 MNESVPDSVE DNGNSVPANG LLVLPDNDHE EGGVGSPQRS NSVESPGGSV HSTRKGFGLK 70 80 90 100 110 120 KWRRIKRDGP VRDEAAPVDD GSKLLKRGLA GLVNPPSKHV EFSSVEARQS SEGSVGSVNM 130 140 150 160 170 180 VHHPGVANGF SPDIGCMFAV GQAFEKSEEH SGNTIGGKNV VGGKVVSGSQ EKLWSDTIKR 190 200 210 220 230 240 ASEERGDIEK EKPCSSLDSD LRSSDFVFST GSVSVGNHGE KDERLTMNYI GGFSNEGQVK 250 260 270 280 290 300 EEVQTYSRSE NGNKEDDGES KKNNNHWADK DALADSIRSF AVLQEVLWKE VQSFQELGKE 310 320 330 340 350 360 SVLLHSNTDE LSSDQPSHQN CKEDNSTSSG SKALILKEKV KLLEHKLEEA RAALEAKEAR 370 380 390 400 410 420 IQELENSKIE SELECIFQRK IETEIEHLML TRSLSSLQVL QETKKLHSLK EDPVSNRGNI 430 440 450 LGKTCKLGFY ILTQLILLVS ILRFLVLQFS PASRLVIPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Anchorage of plant RanGAP to the nuclear envelope involves novel nuclear-pore-associated proteins." Xu X.M., Meulia T., Meier I. Curr. Biol. 17:1157-1163(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INTERACTION WITH RANGAP1; WPP1 AND WPP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024034 Genomic DNA. Translation: BAB02805.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE75338.1. AY045697 mRNA. Translation: AAK74055.1. AY149963 mRNA. Translation: AAN31117.1.
IPI	IPI00520279.
RefSeq	NP_566455.1. NM_112181.4.
UniGene	At.20916.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94AV5. 4 interactions.
STRING	3702.AT3G13360.1-P.
Proteomic databases
PaxDb	Q94AV5.
PRIDE	Q94AV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G13360.1; AT3G13360.1; AT3G13360.
GeneID	820536.
KEGG	ath:AT3G13360.
Organism-specific databases
TAIR	At3g13360.
Phylogenomic databases
eggNOG	NOG281074.
HOGENOM	HOG000093093.
InParanoid	Q9LTT2.
OMA	FEKSEEH.
PhylomeDB	Q94AV5.
ProtClustDB	CLSN2914985.
Gene expression databases
Genevestigator	Q94AV5.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

WIP3_ARATH

Accession

Primary (citable) accession number: Q94AV5
Secondary accession number(s): Q9LTT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program