ID   P2C76_ARATH             Reviewed;         420 AA.
AC   Q94AT1; Q8VZD9; Q9FGM3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 137.
DE   RecName: Full=Probable protein phosphatase 2C 76;
DE            Short=AtPP2C76;
DE            EC=3.1.3.16;
GN   OrderedLocusNames=At5g53140; ORFNames=MFH8.8;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08417.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB025622; BAB08417.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED96312.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM68199.1; -; Genomic_DNA.
DR   EMBL; AY045819; AAK76493.1; -; mRNA.
DR   EMBL; AY065026; AAL57666.1; -; mRNA.
DR   EMBL; AY091360; AAM14299.1; -; mRNA.
DR   EMBL; AY133656; AAM91486.1; -; mRNA.
DR   RefSeq; NP_001329972.1; NM_001345034.1.
DR   RefSeq; NP_568786.1; NM_124693.4.
DR   AlphaFoldDB; Q94AT1; -.
DR   SMR; Q94AT1; -.
DR   BioGRID; 20640; 4.
DR   IntAct; Q94AT1; 2.
DR   MINT; Q94AT1; -.
DR   STRING; 3702.Q94AT1; -.
DR   iPTMnet; Q94AT1; -.
DR   MetOSite; Q94AT1; -.
DR   PaxDb; 3702-AT5G53140-1; -.
DR   ProteomicsDB; 250974; -.
DR   EnsemblPlants; AT5G53140.1; AT5G53140.1; AT5G53140.
DR   EnsemblPlants; AT5G53140.2; AT5G53140.2; AT5G53140.
DR   GeneID; 835395; -.
DR   Gramene; AT5G53140.1; AT5G53140.1; AT5G53140.
DR   Gramene; AT5G53140.2; AT5G53140.2; AT5G53140.
DR   KEGG; ath:AT5G53140; -.
DR   Araport; AT5G53140; -.
DR   TAIR; AT5G53140; -.
DR   eggNOG; KOG0698; Eukaryota.
DR   HOGENOM; CLU_013173_0_6_1; -.
DR   InParanoid; Q94AT1; -.
DR   OMA; KHNPSCN; -.
DR   OrthoDB; 11028at2759; -.
DR   PhylomeDB; Q94AT1; -.
DR   PRO; PR:Q94AT1; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q94AT1; baseline and differential.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0009536; C:plastid; HDA:TAIR.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   CDD; cd00143; PP2Cc; 1.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR000222; PP2C_BS.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   PANTHER; PTHR47992; PROTEIN PHOSPHATASE; 1.
DR   PANTHER; PTHR47992:SF70; PROTEIN-SERINE_THREONINE PHOSPHATASE; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   SMART; SM00331; PP2C_SIG; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS01032; PPM_1; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q94AT1; AT.
PE   2: Evidence at transcript level;
KW   Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW   Reference proteome.
FT   CHAIN           1..420
FT                   /note="Probable protein phosphatase 2C 76"
FT                   /id="PRO_0000367996"
FT   DOMAIN          101..347
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   REGION          353..420
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        372..387
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        391..420
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         137
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         137
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         299
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         338
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        267
FT                   /note="F -> L (in Ref. 3; AAM91486/AAL57666)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   420 AA;  45786 MW;  C312D84D35159052 CRC64;
     MVCSSFIRSF IVQAGCRIGV LAQGRHQFIH IKKTLSVGFG FRTSVIGFRT TSGIGFRTSA
     KMMVDTSAGE KRISLVDMPP EKVDDGGYIG GGWKNDDGSL SCGYCSFRGK RSTMEDFYDI
     KASTIEGQAV CMFGIFDGHG GSRAAEYLKE HLFNNLMKHP QFLTDTKLAL NETYKQTDVA
     FLESEKDTYR DDGSTASAAV LVGNHLYVAN VGDSRTIVSK AGKAIALSDD HKPNRSDERK
     RIESAGGVIM WAGTWRVGGV LAMSRAFGNR MLKQFVVAEP EIQDLEIDHE AELLVLASDG
     LWDVVPNEDA VALAQSEEEP EAAARKLTDT AFSRGSADNI TCIVVKFRHD KTESPKIETN
     AMAESEPELN PTTELEPESN PSTALETESI PKAELESEPD AIPDPKPETE PETKGEKAGE
//