Q94AT1 (P2C76_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 76
Short name=AtPP2C76
EC=3.1.3.16

Gene names

Ordered Locus Names:	At5g53140
ORF Names:	MFH8.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence BAB08417.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Probable protein phosphatase 2C 76		PRO_0000367996
Regions
	Domain	100 – 340	241	PP2C-like
Sites
	Metal binding	137	1	Manganese 1 By similarity
	Metal binding	137	1	Manganese 2 By similarity
	Metal binding	138	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	299	1	Manganese 2 By similarity
	Metal binding	338	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	267	1	F → L in AAM91486. Ref.3
	Sequence conflict	267	1	F → L in AAL57666. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q94AT1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C312D84D35159052	FASTA	420	45,786
	10 20 30 40 50 60 MVCSSFIRSF IVQAGCRIGV LAQGRHQFIH IKKTLSVGFG FRTSVIGFRT TSGIGFRTSA 70 80 90 100 110 120 KMMVDTSAGE KRISLVDMPP EKVDDGGYIG GGWKNDDGSL SCGYCSFRGK RSTMEDFYDI 130 140 150 160 170 180 KASTIEGQAV CMFGIFDGHG GSRAAEYLKE HLFNNLMKHP QFLTDTKLAL NETYKQTDVA 190 200 210 220 230 240 FLESEKDTYR DDGSTASAAV LVGNHLYVAN VGDSRTIVSK AGKAIALSDD HKPNRSDERK 250 260 270 280 290 300 RIESAGGVIM WAGTWRVGGV LAMSRAFGNR MLKQFVVAEP EIQDLEIDHE AELLVLASDG 310 320 330 340 350 360 LWDVVPNEDA VALAQSEEEP EAAARKLTDT AFSRGSADNI TCIVVKFRHD KTESPKIETN 370 380 390 400 410 420 AMAESEPELN PTTELEPESN PSTALETESI PKAELESEPD AIPDPKPETE PETKGEKAGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025622 Genomic DNA. Translation: BAB08417.1. Sequence problems. CP002688 Genomic DNA. Translation: AED96312.1. AY045819 mRNA. Translation: AAK76493.1. AY065026 mRNA. Translation: AAL57666.1. AY091360 mRNA. Translation: AAM14299.1. AY133656 mRNA. Translation: AAM91486.1.
IPI	IPI00530553.
RefSeq	NP_568786.1. NM_124693.3.
UniGene	At.47617.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q94AT1.
SMR	Q94AT1. Positions 100-378.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G53140.1-P.
Proteomic databases
PaxDb	Q94AT1.
PRIDE	Q94AT1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G53140.1; AT5G53140.1; AT5G53140.
GeneID	835395.
KEGG	ath:AT5G53140.
Organism-specific databases
TAIR	At5g53140.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q94AT1.
OMA	ETYKQTD.
PhylomeDB	Q94AT1.
ProtClustDB	CLSN2917757.
Gene expression databases
Genevestigator	Q94AT1.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C76_ARATH

Accession

Primary (citable) accession number: Q94AT1
Secondary accession number(s): Q8VZD9, Q9FGM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program