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Protein

Cyclic nucleotide-gated ion channel 4

Gene

CNGC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as cyclic nucleotide-gated ion channel. Permeable to potassium and sodium in a cyclic nucleotide-dependent fashion (cAMP or cGMP). Might constitute a common downstream component of the signaling pathways leading to hypersensitive response (HR).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei565cNMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi496 – 626cNMPAdd BLAST131

GO - Molecular functioni

GO - Biological processi

  • plant-type hypersensitive response Source: TAIR
  • regulation of membrane potential Source: GO_Central

Keywordsi

Molecular functionCalmodulin-binding, Ion channel, Ligand-gated ion channel
Biological processHypersensitive response, Ion transport, Plant defense, Transport
LigandcAMP, cAMP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2485179 Activation of the phototransduction cascade
R-ATH-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-ATH-5620916 VxPx cargo-targeting to cilium

Protein family/group databases

TCDBi1.A.1.5.7 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated ion channel 4
Short name:
AtCNGC4
Alternative name(s):
Cyclic nucleotide- and calmodulin-regulated ion channel 4
Short name:
AtHLM1
Gene namesi
Name:CNGC4
Synonyms:HLM1
Ordered Locus Names:At5g54250
ORF Names:MDK4.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G54250
TAIRilocus:2162605 AT5G54250

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 92CytoplasmicSequence analysisAdd BLAST92
Transmembranei93 – 113Helical; Name=H1Sequence analysisAdd BLAST21
Topological domaini114 – 126ExtracellularSequence analysisAdd BLAST13
Transmembranei127 – 147Helical; Name=H2Sequence analysisAdd BLAST21
Topological domaini148 – 187CytoplasmicSequence analysisAdd BLAST40
Transmembranei188 – 208Helical; Name=H3Sequence analysisAdd BLAST21
Topological domaini209 – 216ExtracellularSequence analysis8
Transmembranei217 – 237Helical; Name=H4Sequence analysisAdd BLAST21
Topological domaini238 – 251CytoplasmicSequence analysisAdd BLAST14
Transmembranei252 – 272Helical; Name=H5Sequence analysisAdd BLAST21
Topological domaini273 – 392ExtracellularSequence analysisAdd BLAST120
Transmembranei393 – 413Helical; Name=H6Sequence analysisAdd BLAST21
Topological domaini414 – 694CytoplasmicSequence analysisAdd BLAST281

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Loss-of-function mutation results in the loss of the hypersensitive response leading to broad spectrum disease resistance, and displays a lesion-mimic phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193321 – 694Cyclic nucleotide-gated ion channel 4Add BLAST694

Proteomic databases

PaxDbiQ94AS9

PTM databases

iPTMnetiQ94AS9

Expressioni

Inductioni

Induced by both ethylene and methyl jasmonate treatments, or after pathogen attack.

Gene expression databases

ExpressionAtlasiQ94AS9 baseline and differential
GenevisibleiQ94AS9 AT

Interactioni

Subunit structurei

Homotetramer or heterotetramer.Curated

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G54250.1

Structurei

3D structure databases

ProteinModelPortaliQ94AS9
SMRiQ94AS9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini631 – 660IQAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni610 – 626Calmodulin-bindingBy similarityAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 52Glu-richAdd BLAST28

Domaini

The binding of calmodulin to the C-terminus might interfere with cyclic nucleotide binding and thus channel activation.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOGENOMiHOG000238338
InParanoidiQ94AS9
OMAiWGETINA
OrthoDBiEOG0936045S
PhylomeDBiQ94AS9

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00520 Ion_trans, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SUPFAMiSSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS50042 CNMP_BINDING_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q94AS9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATEQEFTRA SRFSRDSSSV GYYSEEDNTE EEDEEEEEME EIEEEEEEEE
60 70 80 90 100
EEDPRIGLTC GGRRNGSSNN NKWMMLGRIL DPRSKWVREW NKVFLLVCAT
110 120 130 140 150
GLFVDPLFLY TLSVSDTCMC LLVDGWLALT VTALRSMTDL LHLWNIWIQF
160 170 180 190 200
KIARRWPYPG GDSDGDTNKG GGTRGSTRVA PPYVKKNGFF FDLFVILPLP
210 220 230 240 250
QVVLWVVIPS LLKRGSVTLV VSVLLVTFLF QYLPKIYHSI RHLRRNATLS
260 270 280 290 300
GYIFGTVWWG IALNMIAYFV AAHAAGACWY LLGVQRSAKC LKEQCENTIG
310 320 330 340 350
CDLRMLSCKE PVYYGTTVMV LDRARLAWAQ NHQARSVCLD INTNYTYGAY
360 370 380 390 400
QWTIQLVSSE SRLEKILFPI FWGLMTLSTF GNLESTTEWS EVVFNIIVLT
410 420 430 440 450
SGLLLVTMLI GNIKVFLHAT TSKKQAMHLK MRNIEWWMKK RHLPIGFRQR
460 470 480 490 500
VRNYERQRWA AMRGVDECEM VQNLPEGLRR DIKYHLCLDL VRQVPLFQHM
510 520 530 540 550
DDLVLENICD RVKSLIFTKG ETIQKEGDAV QRMLFVVRGH LQSSQLLRDG
560 570 580 590 600
VKSCCMLGPG NFSGDELLSW CLRRPFVERL PPSSSTLVTL ETTEAFGLDA
610 620 630 640 650
EDVKYVTQHF RYTFVNEKVK RSARYYSPGW RTWAAVAVQL AWRRYKHRLT
660 670 680 690
LTSLSFIRPR RPLSRCASLG EDKLRLYAAI LTSPKPNPDD FDDY
Length:694
Mass (Da):80,081
Last modified:November 21, 2003 - v2
Checksum:iE3F843AE1B0F1EA0
GO
Isoform 2 (identifier: Q94AS9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-293: AAGACWYLLGVQRSAKCLKE → VSYLINCFVLLNILVNTKFQ
     294-694: Missing.

Note: No experimental confirmation available.
Show »
Length:293
Mass (Da):33,665
Checksum:iCBC9AA9DF55C9EE4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008985274 – 293AAGAC…KCLKE → VSYLINCFVLLNILVNTKFQ in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_008986294 – 694Missing in isoform 2. 1 PublicationAdd BLAST401

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17912 mRNA Translation: CAB40129.1
AB010695 Genomic DNA Translation: BAB10748.1
CP002688 Genomic DNA Translation: AED96473.1
CP002688 Genomic DNA Translation: AED96474.1
CP002688 Genomic DNA Translation: ANM70509.1
AY045822 mRNA Translation: AAK76496.1
AY057691 mRNA Translation: AAL15321.1
AY133734 mRNA Translation: AAM91668.1
PIRiT52574
RefSeqiNP_001332114.1, NM_001345080.1 [Q94AS9-1]
NP_200236.1, NM_124805.3 [Q94AS9-1]
NP_851188.1, NM_180857.2 [Q94AS9-1]
UniGeneiAt.401

Genome annotation databases

EnsemblPlantsiAT5G54250.1; AT5G54250.1; AT5G54250 [Q94AS9-1]
AT5G54250.2; AT5G54250.2; AT5G54250 [Q94AS9-1]
AT5G54250.4; AT5G54250.4; AT5G54250 [Q94AS9-1]
GeneIDi835513
GrameneiAT5G54250.1; AT5G54250.1; AT5G54250 [Q94AS9-1]
AT5G54250.2; AT5G54250.2; AT5G54250 [Q94AS9-1]
AT5G54250.4; AT5G54250.4; AT5G54250 [Q94AS9-1]
KEGGiath:AT5G54250

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNGC4_ARATH
AccessioniPrimary (citable) accession number: Q94AS9
Secondary accession number(s): Q9XFS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 21, 2003
Last modified: April 25, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health