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Q94AR8 (LEUC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase
EC=4.2.1.33
Alternative name(s):
Isopropylmalate isomerase large subunit 1
Short name=AtIIL1
Gene names
Name:IIL1
Ordered Locus Names:At4g13430
ORF Names:T9E8.170
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. Ref.1

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.

Subcellular location

Plastid › chloroplast Potential Ref.5.

Miscellaneous

The environment dependent reduction in IIL1 activity and severely impaired growth of cv. Bur-0 are caused by a triplet repeat expansion in the third intron of the gene.

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Sequence caution

The sequence CAB40778.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB78385.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7676Chloroplast Potential
Chain77 – 5094333-isopropylmalate dehydratase
PRO_0000366939

Sites

Metal binding3761Iron-sulfur (4Fe-4S) By similarity
Metal binding4451Iron-sulfur (4Fe-4S) By similarity
Metal binding4481Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q94AR8 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 6CE200D838894ECC

FASTA50955,014
        10         20         30         40         50         60 
MASVISSSPF LCKSSSKSDL GISSFPKSSQ ISIHRCQKKS ISRKIVSVMA PQKDRSPGTT 

        70         80         90        100        110        120 
GSVKTGMTMT EKILARASEK SLVVPGDNIW VNVDVLMTHD VCGPGAFGIF KREFGEKAKV 

       130        140        150        160        170        180 
WDPEKIVVIP DHYIFTADKR ANRNVDIMRE HCREQNIKYF YDITDLGNFK ANPDYKGVCH 

       190        200        210        220        230        240 
VALAQEGHCR PGEVLLGTDS HTCTAGAFGQ FATGIGNTDA GFVLGTGKIL LKVPPTMRFI 

       250        260        270        280        290        300 
LDGEMPSYLQ AKDLILQIIG EISVAGATYK TMEFSGTTIE SLSMEERMTL CNMVVEAGGK 

       310        320        330        340        350        360 
NGVIPPDATT LNYVENRTSV PFEPVYSDGN ASFVADYRFD VSKLEPVVAK PHSPDNRALA 

       370        380        390        400        410        420 
RECKDVKIDR VYIGSCTGGK TEDFMAAAKL FHAAGRKVKV PTFLVPATQK VWMDVYALPV 

       430        440        450        460        470        480 
PGAGGKTCAQ IFEEAGCDTP ASPSCGACLG GPADTYARLN EPQVCVSTTN RNFPGRMGHK 

       490        500 
EGQIYLASPY TAAASALTGR VADPREFLQ

« Hide

References

« Hide 'large scale' references
[1]"A genetic defect caused by a triplet repeat expansion in Arabidopsis thaliana."
Sureshkumar S., Todesco M., Schneeberger K., Harilal R., Balasubramanian S., Weigel D.
Science 323:1060-1063(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: cv. Bur-0, cv. Columbia and cv. Pf-0.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL049608 Genomic DNA. Translation: CAB40778.1. Sequence problems.
AL161536 Genomic DNA. Translation: CAB78385.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83278.1.
AY045842 mRNA. Translation: AAK76516.1.
AY117151 mRNA. Translation: AAM51226.1.
IPIIPI00543884.
PIRT06300.
RefSeqNP_567405.1. NM_117417.3.
UniGeneAt.24538.
At.67680.

3D structure databases

ProteinModelPortalQ94AR8.
SMRQ94AR8. Positions 122-507.
ModBaseSearch...

Protein-protein interaction databases

STRING3702.AT4G13430.1-P.

Proteomic databases

PaxDbQ94AR8.
PRIDEQ94AR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G13430.1; AT4G13430.1; AT4G13430.
GeneID826975.
KEGGath:AT4G13430.

Organism-specific databases

TAIRAt4g13430.

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226971.
InParanoidQ94AR8.
KOK01703.
OMAVIPFDHQ.
PhylomeDBQ94AR8.
ProtClustDBCLSN2689413.

Enzyme and pathway databases

BioCycARA:AT4G13430-MONOMER.
MetaCyc:AT4G13430-MONOMER.
UniPathwayUPA00048; UER00071.

Gene expression databases

ArrayExpressQ94AR8.
GenevestigatorQ94AR8.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR006251. Homoacnase/IPMdehydase_lsu.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PTHR11670:SF6. PTHR11670:SF6. 1 hit.
PfamPF00330. Aconitase. 2 hits.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. Aconitase_N. 1 hit.
TIGRFAMsTIGR01343. hacA_fam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLEUC_ARATH
AccessionPrimary (citable) accession number: Q94AR8
Secondary accession number(s): Q9T0L4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 1, 2001
Last modified: May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents