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Protein

Allantoinase

Gene

ALN

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoate by hydrolytic cleavage of the five-member hydantoin ring. Catalyzes the first step of the ureide allantoin degradation followed by the sequential activity of AAH, UGLYAH and UAH which allows a complete purine berakdown without the intermediate generation of urea.3 Publications

Catalytic activityi

(S)-allantoin + H2O = allantoate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=5.47 mM for allantoin1 Publication

    Pathwayi: (S)-allantoin degradation

    This protein is involved in step 1 of the subpathway that synthesizes allantoate from (S)-allantoin.
    Proteins known to be involved in this subpathway in this organism are:
    1. Allantoinase (ALN)
    This subpathway is part of the pathway (S)-allantoin degradation, which is itself part of Nitrogen metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes allantoate from (S)-allantoin, the pathway (S)-allantoin degradation and in Nitrogen metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi105 – 1051Zinc 1By similarity
    Metal bindingi107 – 1071Zinc 1By similarity
    Metal bindingi195 – 1951Zinc 1; via carbamate groupBy similarity
    Metal bindingi195 – 1951Zinc 2; via carbamate groupBy similarity
    Metal bindingi231 – 2311Zinc 2By similarity
    Metal bindingi292 – 2921Zinc 2By similarity
    Metal bindingi366 – 3661Zinc 1By similarity

    GO - Molecular functioni

    • allantoinase activity Source: TAIR
    • cobalt ion binding Source: InterPro
    • zinc ion binding Source: InterPro

    GO - Biological processi

    • allantoin catabolic process Source: UniProtKB-UniPathway
    • cellular response to nitrogen starvation Source: TAIR
    • purine nucleobase catabolic process Source: TAIR
    • ureide catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Purine metabolism

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT4G04955-MONOMER.
    UniPathwayiUPA00395; UER00653.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Allantoinase (EC:3.5.2.5)
    Short name:
    AtALN
    Gene namesi
    Name:ALN
    Ordered Locus Names:At4g04955
    ORF Names:T1J1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G04955.

    Subcellular locationi

    GO - Cellular componenti

    • endoplasmic reticulum Source: TAIR
    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants are unable to grow on a medium containing allantoin as the sole nitrogen source, accumulate allantoin and have increased tolerance to drought and osmotic stresses.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506AllantoinasePRO_0000430041Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei195 – 1951N6-carboxylysineBy similarity

    Post-translational modificationi

    Carbamoylation allows a single lysine to coordinate two zinc ions.By similarity

    Proteomic databases

    PaxDbiQ94AP0.
    PRIDEiQ94AP0.

    Expressioni

    Gene expression databases

    GenevisibleiQ94AP0. AT.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT4G04955.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94AP0.
    SMRiQ94AP0. Positions 47-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DHOase family. Allantoinase subfamily.Curated

    Phylogenomic databases

    eggNOGiKOG2584. Eukaryota.
    COG0044. LUCA.
    HOGENOMiHOG000219146.
    InParanoidiQ94AP0.
    KOiK01466.
    OMAiEMIPPIT.
    OrthoDBiEOG093607UJ.
    PhylomeDBiQ94AP0.

    Family and domain databases

    Gene3Di2.30.40.10. 1 hit.
    InterProiIPR017593. Allantoinase.
    IPR006680. Amidohydro-rel.
    IPR011059. Metal-dep_hydrolase_composite.
    IPR032466. Metal_Hydrolase.
    [Graphical view]
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 2 hits.
    SSF51556. SSF51556. 1 hit.
    TIGRFAMsiTIGR03178. allantoinase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q94AP0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MERTLLQWRL LPLLALIVAL FSFFFASPRS LQGNNKCSLL PHDHYWISSK
    60 70 80 90 100
    RIVTPNGLIS GSVEVKGGII VSVVKEVDWH KSQRSRVKVI DYGEAVLMPG
    110 120 130 140 150
    LIDVHVHLDD PGRSEWEGFP SGTKAAAAGG ITTLVDMPLN SFPSTVSPET
    160 170 180 190 200
    LKLKIEAAKN RIHVDVGFWG GLVPDNALNS SALESLLDAG VLGLKSFMCP
    210 220 230 240 250
    SGINDFPMTN ITHIKEGLSV LAKYKRPLLV HAEIERDLEI EDGSENDPRS
    260 270 280 290 300
    YLTYLKTRPT SWEEGAIRNL LSVTENTRIG GSAEGAHLHI VHLSDASSSL
    310 320 330 340 350
    DLIKEAKGKG DSVTVETCPH YLAFSAEEIP EGDTRFKCSP PIRDAANREK
    360 370 380 390 400
    LWEALMEGDI DMLSSDHSPT KPELKLMSDG NFLKAWGGIS SLQFVLPITW
    410 420 430 440 450
    SYGKKYGVTL EQVTSWWSDR PSKLAGLHSK GAVTVGKHAD LVVWEPEAEF
    460 470 480 490 500
    DVDEDHPIHF KHPSISAYLG RRLSGKVVST FVRGNLVFGE GKHASDACGS

    LQLATT
    Length:506
    Mass (Da):55,432
    Last modified:December 1, 2001 - v1
    Checksum:i09018BBE5CAB5428
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti426 – 4261G → R in AAM63760 (Ref. 4) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF128393 Genomic DNA. No translation available.
    CP002687 Genomic DNA. Translation: AEE82448.1.
    AY045901 mRNA. Translation: AAK76575.1.
    AY113948 mRNA. Translation: AAM44996.1.
    AY086706 mRNA. Translation: AAM63760.1.
    RefSeqiNP_567276.1. NM_116734.2.
    UniGeneiAt.26179.

    Genome annotation databases

    EnsemblPlantsiAT4G04955.1; AT4G04955.1; AT4G04955.
    GeneIDi825836.
    GrameneiAT4G04955.1; AT4G04955.1; AT4G04955.
    KEGGiath:AT4G04955.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF128393 Genomic DNA. No translation available.
    CP002687 Genomic DNA. Translation: AEE82448.1.
    AY045901 mRNA. Translation: AAK76575.1.
    AY113948 mRNA. Translation: AAM44996.1.
    AY086706 mRNA. Translation: AAM63760.1.
    RefSeqiNP_567276.1. NM_116734.2.
    UniGeneiAt.26179.

    3D structure databases

    ProteinModelPortaliQ94AP0.
    SMRiQ94AP0. Positions 47-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G04955.1.

    Proteomic databases

    PaxDbiQ94AP0.
    PRIDEiQ94AP0.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G04955.1; AT4G04955.1; AT4G04955.
    GeneIDi825836.
    GrameneiAT4G04955.1; AT4G04955.1; AT4G04955.
    KEGGiath:AT4G04955.

    Organism-specific databases

    TAIRiAT4G04955.

    Phylogenomic databases

    eggNOGiKOG2584. Eukaryota.
    COG0044. LUCA.
    HOGENOMiHOG000219146.
    InParanoidiQ94AP0.
    KOiK01466.
    OMAiEMIPPIT.
    OrthoDBiEOG093607UJ.
    PhylomeDBiQ94AP0.

    Enzyme and pathway databases

    UniPathwayiUPA00395; UER00653.
    BioCyciARA:AT4G04955-MONOMER.

    Miscellaneous databases

    PROiQ94AP0.

    Gene expression databases

    GenevisibleiQ94AP0. AT.

    Family and domain databases

    Gene3Di2.30.40.10. 1 hit.
    InterProiIPR017593. Allantoinase.
    IPR006680. Amidohydro-rel.
    IPR011059. Metal-dep_hydrolase_composite.
    IPR032466. Metal_Hydrolase.
    [Graphical view]
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 2 hits.
    SSF51556. SSF51556. 1 hit.
    TIGRFAMsiTIGR03178. allantoinase. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiALN_ARATH
    AccessioniPrimary (citable) accession number: Q94AP0
    Secondary accession number(s): Q8LCA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 3, 2014
    Last sequence update: December 1, 2001
    Last modified: September 7, 2016
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In Arabidopsis the intermediary metabolite allantoin plays a role in abiotic stress tolerance via activation of abscisic acid (ABA) metabolism.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.