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Protein

Allantoinase

Gene

ALN

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoate by hydrolytic cleavage of the five-member hydantoin ring. Catalyzes the first step of the ureide allantoin degradation followed by the sequential activity of AAH, UGLYAH and UAH which allows a complete purine berakdown without the intermediate generation of urea.3 Publications

Miscellaneous

In Arabidopsis the intermediary metabolite allantoin plays a role in abiotic stress tolerance via activation of abscisic acid (ABA) metabolism.1 Publication

Catalytic activityi

(S)-allantoin + H2O = allantoate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=5.47 mM for allantoin1 Publication

    Pathwayi: (S)-allantoin degradation

    This protein is involved in step 1 of the subpathway that synthesizes allantoate from (S)-allantoin.
    Proteins known to be involved in this subpathway in this organism are:
    1. Allantoinase (ALN)
    This subpathway is part of the pathway (S)-allantoin degradation, which is itself part of Nitrogen metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes allantoate from (S)-allantoin, the pathway (S)-allantoin degradation and in Nitrogen metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi105Zinc 1By similarity1
    Metal bindingi107Zinc 1By similarity1
    Metal bindingi195Zinc 1; via carbamate groupBy similarity1
    Metal bindingi195Zinc 2; via carbamate groupBy similarity1
    Metal bindingi231Zinc 2By similarity1
    Metal bindingi292Zinc 2By similarity1
    Metal bindingi366Zinc 1By similarity1

    GO - Molecular functioni

    • allantoinase activity Source: TAIR
    • cobalt ion binding Source: InterPro
    • zinc ion binding Source: InterPro

    GO - Biological processi

    • allantoin catabolic process Source: UniProtKB-UniPathway
    • cellular response to nitrogen starvation Source: TAIR
    • purine nucleobase catabolic process Source: TAIR
    • ureide catabolic process Source: TAIR

    Keywordsi

    Molecular functionHydrolase
    Biological processPurine metabolism
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT4G04955-MONOMER
    UniPathwayiUPA00395; UER00653

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Allantoinase (EC:3.5.2.5)
    Short name:
    AtALN
    Gene namesi
    Name:ALN
    Ordered Locus Names:At4g04955
    ORF Names:T1J1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G04955
    TAIRilocus:505006432 AT4G04955

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants are unable to grow on a medium containing allantoin as the sole nitrogen source, accumulate allantoin and have increased tolerance to drought and osmotic stresses.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004300411 – 506AllantoinaseAdd BLAST506

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei195N6-carboxylysineBy similarity1

    Post-translational modificationi

    Carbamoylation allows a single lysine to coordinate two zinc ions.By similarity

    Proteomic databases

    PaxDbiQ94AP0

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ94AP0 differential
    GenevisibleiQ94AP0 AT

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT4G04955.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ94AP0
    SMRiQ94AP0
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG2584 Eukaryota
    COG0044 LUCA
    HOGENOMiHOG000219146
    InParanoidiQ94AP0
    KOiK01466
    OMAiQHAQEPR
    OrthoDBiEOG093607UJ
    PhylomeDBiQ94AP0

    Family and domain databases

    Gene3Di2.30.40.10, 3 hits
    InterProiView protein in InterPro
    IPR017593 Allantoinase
    IPR006680 Amidohydro-rel
    IPR011059 Metal-dep_hydrolase_composite
    IPR032466 Metal_Hydrolase
    PfamiView protein in Pfam
    PF01979 Amidohydro_1, 1 hit
    SUPFAMiSSF51338 SSF51338, 2 hits
    SSF51556 SSF51556, 1 hit
    TIGRFAMsiTIGR03178 allantoinase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q94AP0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MERTLLQWRL LPLLALIVAL FSFFFASPRS LQGNNKCSLL PHDHYWISSK
    60 70 80 90 100
    RIVTPNGLIS GSVEVKGGII VSVVKEVDWH KSQRSRVKVI DYGEAVLMPG
    110 120 130 140 150
    LIDVHVHLDD PGRSEWEGFP SGTKAAAAGG ITTLVDMPLN SFPSTVSPET
    160 170 180 190 200
    LKLKIEAAKN RIHVDVGFWG GLVPDNALNS SALESLLDAG VLGLKSFMCP
    210 220 230 240 250
    SGINDFPMTN ITHIKEGLSV LAKYKRPLLV HAEIERDLEI EDGSENDPRS
    260 270 280 290 300
    YLTYLKTRPT SWEEGAIRNL LSVTENTRIG GSAEGAHLHI VHLSDASSSL
    310 320 330 340 350
    DLIKEAKGKG DSVTVETCPH YLAFSAEEIP EGDTRFKCSP PIRDAANREK
    360 370 380 390 400
    LWEALMEGDI DMLSSDHSPT KPELKLMSDG NFLKAWGGIS SLQFVLPITW
    410 420 430 440 450
    SYGKKYGVTL EQVTSWWSDR PSKLAGLHSK GAVTVGKHAD LVVWEPEAEF
    460 470 480 490 500
    DVDEDHPIHF KHPSISAYLG RRLSGKVVST FVRGNLVFGE GKHASDACGS

    LQLATT
    Length:506
    Mass (Da):55,432
    Last modified:December 1, 2001 - v1
    Checksum:i09018BBE5CAB5428
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti426G → R in AAM63760 (Ref. 4) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF128393 Genomic DNA No translation available.
    CP002687 Genomic DNA Translation: AEE82448.1
    AY045901 mRNA Translation: AAK76575.1
    AY113948 mRNA Translation: AAM44996.1
    AY086706 mRNA Translation: AAM63760.1
    RefSeqiNP_567276.1, NM_116734.3
    UniGeneiAt.26179

    Genome annotation databases

    EnsemblPlantsiAT4G04955.1; AT4G04955.1; AT4G04955
    GeneIDi825836
    GrameneiAT4G04955.1; AT4G04955.1; AT4G04955
    KEGGiath:AT4G04955

    Similar proteinsi

    Entry informationi

    Entry nameiALN_ARATH
    AccessioniPrimary (citable) accession number: Q94AP0
    Secondary accession number(s): Q8LCA4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
    Last sequence update: December 1, 2001
    Last modified: May 23, 2018
    This is version 110 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

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