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Protein

Choline transporter protein 1

Gene

CHER1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Choline transporter involved in the regulation of choline metabolite homeostasis during root and phloem development (PubMed:25008948). Necessary for secondary plasmodesmata (PD) formation and development. Required for PD localization of MP17, the luteoviral movement protein (PubMed:27743414). Modulates phloem morphogenesis and conductivity. Required for procambium maintenance and sieve plate development (e.g. sieve plate and sieve pore elaboration) to mediate long-distance cell-to-cell communication via symplastic transport through the phloem (PubMed:25008948).2 Publications

GO - Molecular functioni

  • choline transmembrane transporter activity Source: TAIR

GO - Biological processi

  • choline transport Source: TAIR
  • phloem development Source: TAIR
  • plasmodesma organization Source: UniProtKB
  • procambium histogenesis Source: UniProtKB
  • root development Source: TAIR
  • sieve element differentiation Source: TAIR
  • xylem and phloem pattern formation Source: TAIR

Keywordsi

Molecular functionDevelopmental protein
Biological processTransport

Enzyme and pathway databases

ReactomeiR-ATH-1483191. Synthesis of PC.
R-ATH-425366. Transport of bile salts and organic acids, metal ions and amine compounds.
R-ATH-6798163. Choline catabolism.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline transporter protein 11 Publication
Short name:
AtCTL11 Publication
Gene namesi
Name:CHER11 Publication
Synonyms:CTL11 Publication
Ordered Locus Names:At3g15380Imported
ORF Names:MJK13.4Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G15380.
TAIRilocus:2090171. AT3G15380.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicCuratedAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 241ExtracellularCuratedAdd BLAST187
Transmembranei242 – 262HelicalSequence analysisAdd BLAST21
Topological domaini263 – 264CytoplasmicCurated2
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 322ExtracellularCuratedAdd BLAST37
Transmembranei323 – 343HelicalSequence analysisAdd BLAST21
Topological domaini344 – 364CytoplasmicCuratedAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Topological domaini386 – 432ExtracellularCuratedAdd BLAST47
Transmembranei433 – 453HelicalSequence analysisAdd BLAST21
Topological domaini454 – 493CytoplasmicCuratedAdd BLAST40
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 595ExtracellularCuratedAdd BLAST81
Transmembranei596 – 616HelicalSequence analysisAdd BLAST21
Topological domaini617 – 633CytoplasmicCuratedAdd BLAST17
Transmembranei634 – 654HelicalSequence analysisAdd BLAST21
Topological domaini655 – 700ExtracellularCuratedAdd BLAST46

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Altered choline metabolite profile. Several phenotypic abnormalities (e.g. dwarf with defects in both shoot and root architecture), including reduced pore density and altered pore structure in the sieve areas, associated with defective symplastic transport through phloem. Increased number of sieve elements (SE)-like cells instead of two companion cells (CCs) and two SEs. Defects in procambium maintenance and phloem patterning. Abnormal retaining of desmotubules in the symplastic space in sieve pores (PubMed:25008948). In cher1-4, impaired secondary plasmodesmata (PD) formation and development leading to starch and soluble sugars excess accumulation, and stunted growth. Altered PD localization of the luteoviral movement protein MP17. Reduced level of choline and phosphocholine (PubMed:27743414).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi247G → E in cher1-5; impaired secondary plasmodesmata (PD) formation and development leading to starch and soluble sugars excess accumulation, stunted growth. Altered PD localization of the luteoviral movement protein MP17. Abnormal subcellular localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004400371 – 700Choline transporter protein 1Add BLAST700

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi163N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi194N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi403N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

ExpressionAtlasiQ94AN2. baseline and differential.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1362. Eukaryota.
ENOG410XS0P. LUCA.
HOGENOMiHOG000241130.
KOiK15377.
OMAiVYWIMTL.
OrthoDBiEOG0936037I.

Family and domain databases

InterProiView protein in InterPro
IPR007603. Choline_transptr-like.
PANTHERiPTHR12385. PTHR12385. 1 hit.
PfamiView protein in Pfam
PF04515. Choline_transpo. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94AN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGPLGAVIG RYTSSDGSAP NDGIIKHNRK CRDITFLIIF IAFWVSMIVN
60 70 80 90 100
SSFGFNQGNP LRLTYGLDYE GNVCGSKHRH RDLTQLELRY WLNPNQVYES
110 120 130 140 150
GLKDGELKLA NARTICLLDC PAPSDDTLNW VCDYPDGEIR LKMNDWIDRN
160 170 180 190 200
YDYFEFLTPE MRNSSLQLQG PCYPVIFPSV NVYWSCQYIA RASNSSLRHW
210 220 230 240 250
QQMGGVNIQE DMIIDKSIRR SMNSRASVLK RYVADIGKSW PVLIVCGGLV
260 270 280 290 300
PLFLSIIWLL LIRHFVAAMP WITVVLFNML LISVTVFYYL KAGWIGNDAV
310 320 330 340 350
TPIIGEHDPY FHVYGRELTH VRGVAILMTF ISVVAILTSI AIIRRILMAT
360 370 380 390 400
SVLKVAAKVI GEVQALIIFP AIPFAMLAIF YMFWISAALH LFSSGQVVQN
410 420 430 440 450
NCNNTNCCAY DLVLKKVNCD RCCGYSIHYT PHITIAIFFH LFGCYWATQF
460 470 480 490 500
FIASSATVIA GSVASYYWAQ GEASPEIPFL PVFASMKRLA RYNLGSVALG
510 520 530 540 550
SLIVSFVESV RFILEAIRRK TKVSGTIPDH WFWRMAHYTS RGCLKSVEWT
560 570 580 590 600
IKSVNRNAYI MIAITGKSFC KSSAIATELI ISNILRIGKV NVIGDVILFL
610 620 630 640 650
GKLCVSLFSA LFGFLMLDSH RYRASHNKVS SPLLPVLACW ALGYIVATLF
660 670 680 690 700
FAVVEMSIDT IILSFCQDSE ENQGNAQHAP PLLLETLDSN QEEEVQSLTH
Length:700
Mass (Da):78,737
Last modified:December 1, 2001 - v1
Checksum:iDF9F0E38C4E36C25
GO

Sequence cautioni

The sequence AAF35404 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAB02367 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022218 Genomic DNA. Translation: BAB02367.1. Sequence problems.
AC024081 Genomic DNA. Translation: AAF35404.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75661.1.
AY045917 mRNA. Translation: AAK76591.1.
BT001974 mRNA. Translation: AAN71973.1.
RefSeqiNP_566511.1. NM_112406.5.
UniGeneiAt.11972.
At.49625.

Genome annotation databases

EnsemblPlantsiAT3G15380.1; AT3G15380.1; AT3G15380.
GeneIDi820777.
GrameneiAT3G15380.1; AT3G15380.1; AT3G15380.
KEGGiath:AT3G15380.

Similar proteinsi

Entry informationi

Entry nameiCHER1_ARATH
AccessioniPrimary (citable) accession number: Q94AN2
Secondary accession number(s): Q9LUQ4, Q9M7X1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
Last sequence update: December 1, 2001
Last modified: October 25, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families