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Protein

Probable polygalacturonase At1g80170

Gene

At1g80170

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei249Proton donorPROSITE-ProRule annotation1
Active sitei272PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G80170-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable polygalacturonase At1g80170 (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Pectinase At1g80170
Gene namesi
Ordered Locus Names:At1g80170
ORF Names:F18B13.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G80170.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB-KW
  • plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000036791729 – 444Probable polygalacturonase At1g80170Add BLAST416

Proteomic databases

PaxDbiQ94AJ5.

PTM databases

iPTMnetiQ94AJ5.

Expressioni

Tissue specificityi

Expressed in young, mature and dehiscing anthers. Found in stems, but not in roots or in abscission zone of floral organs.2 Publications

Gene expression databases

GenevisibleiQ94AJ5. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NIP1-1Q8VZW12EBI-4457426,EBI-4424378

Protein-protein interaction databases

BioGridi29575. 2 interactors.
IntActiQ94AJ5. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ94AJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati208 – 234PbH1 1Add BLAST27
Repeati235 – 256PbH1 2Add BLAST22
Repeati258 – 278PbH1 3Add BLAST21
Repeati288 – 309PbH1 4Add BLAST22
Repeati317 – 338PbH1 5Add BLAST22
Repeati351 – 378PbH1 6Add BLAST28

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IJY9. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ94AJ5.
OMAiGTVNGMG.
OrthoDBiEOG09360ADQ.
PhylomeDBiQ94AJ5.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94AJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYSRGGTLV TLLLLLVVAS SLALTANANS FESLLQLPRR QSRSTRPRSE
60 70 80 90 100
RLLHVGNFGA KGNGVTDDTK AFADAWKTAC SSKVKTRILV PENYTCLLRP
110 120 130 140 150
IDLSGPCKAR LTLQISGTII APNDPDVWEG LNRRKWLYFH GLSRLTVEGG
160 170 180 190 200
GTVNGMGQEW WERSCKHNHS NPCRGAPTAL TFHKCKNMRV ENLNVIDSQQ
210 220 230 240 250
MHIALTSCRR VTISGLKVIA PATSPNTDGI HISVSRGIVI DNTTVSTGDD
260 270 280 290 300
CISIVKNSTQ ISISNIICGP GHGISIGSLG KSKSWEEVRD ITVDTAIISD
310 320 330 340 350
TANGVRIKTW QGGSGLVSKI IFRNIKMNNV SNPIIIDQYY CDSRKPCANQ
360 370 380 390 400
TSAISIENIS FVHVRGTSAS KEAIKISCSD SSPCRNILLQ DIDLEPSNGD
410 420 430 440
GFTESFCWEA YGSSSGQVYP PPCLSDDTSF LEQSVQSGIT SAYL
Length:444
Mass (Da):48,172
Last modified:December 1, 2001 - v1
Checksum:i8FC52117E96B6418
GO

Sequence cautioni

The sequence AAD55489 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009322 Genomic DNA. Translation: AAD55489.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36366.1.
AY046002 mRNA. Translation: AAK76676.1.
AY142668 mRNA. Translation: AAN13206.1.
PIRiD96833.
RefSeqiNP_565232.1. NM_106666.4.
UniGeneiAt.11620.

Genome annotation databases

EnsemblPlantsiAT1G80170.1; AT1G80170.1; AT1G80170.
GeneIDi844357.
GrameneiAT1G80170.1; AT1G80170.1; AT1G80170.
KEGGiath:AT1G80170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009322 Genomic DNA. Translation: AAD55489.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36366.1.
AY046002 mRNA. Translation: AAK76676.1.
AY142668 mRNA. Translation: AAN13206.1.
PIRiD96833.
RefSeqiNP_565232.1. NM_106666.4.
UniGeneiAt.11620.

3D structure databases

ProteinModelPortaliQ94AJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29575. 2 interactors.
IntActiQ94AJ5. 2 interactors.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

PTM databases

iPTMnetiQ94AJ5.

Proteomic databases

PaxDbiQ94AJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G80170.1; AT1G80170.1; AT1G80170.
GeneIDi844357.
GrameneiAT1G80170.1; AT1G80170.1; AT1G80170.
KEGGiath:AT1G80170.

Organism-specific databases

TAIRiAT1G80170.

Phylogenomic databases

eggNOGiENOG410IJY9. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ94AJ5.
OMAiGTVNGMG.
OrthoDBiEOG09360ADQ.
PhylomeDBiQ94AJ5.

Enzyme and pathway databases

BioCyciARA:AT1G80170-MONOMER.

Miscellaneous databases

PROiQ94AJ5.

Gene expression databases

GenevisibleiQ94AJ5. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR5_ARATH
AccessioniPrimary (citable) accession number: Q94AJ5
Secondary accession number(s): Q9SSC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.