Q94AJ5 (PGLR5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable polygalacturonase At1g80170
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase At1g80170

Gene names

Ordered Locus Names:	At1g80170
ORF Names:	F18B13.25

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in young, mature and dehiscing anthers. Found in stems, but not in roots or in abscission zone of floral organs. Ref.4 Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.
Sequence caution	The sequence AAD55489.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NIP1-1	Q8VZW1	2	EBI-4457426,EBI-4424378

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 444	416	Probable polygalacturonase At1g80170		PRO_0000367917
Regions
	Repeat	208 – 234	27	PbH1 1
	Repeat	235 – 256	22	PbH1 2
	Repeat	258 – 278	21	PbH1 3
	Repeat	288 – 309	22	PbH1 4
	Repeat	317 – 338	22	PbH1 5
	Repeat	351 – 378	28	PbH1 6
Sites
	Active site	249	1	Proton donor By similarity
	Active site	272	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q94AJ5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8FC52117E96B6418	FASTA	444	48,172
	10 20 30 40 50 60 MSYSRGGTLV TLLLLLVVAS SLALTANANS FESLLQLPRR QSRSTRPRSE RLLHVGNFGA 70 80 90 100 110 120 KGNGVTDDTK AFADAWKTAC SSKVKTRILV PENYTCLLRP IDLSGPCKAR LTLQISGTII 130 140 150 160 170 180 APNDPDVWEG LNRRKWLYFH GLSRLTVEGG GTVNGMGQEW WERSCKHNHS NPCRGAPTAL 190 200 210 220 230 240 TFHKCKNMRV ENLNVIDSQQ MHIALTSCRR VTISGLKVIA PATSPNTDGI HISVSRGIVI 250 260 270 280 290 300 DNTTVSTGDD CISIVKNSTQ ISISNIICGP GHGISIGSLG KSKSWEEVRD ITVDTAIISD 310 320 330 340 350 360 TANGVRIKTW QGGSGLVSKI IFRNIKMNNV SNPIIIDQYY CDSRKPCANQ TSAISIENIS 370 380 390 400 410 420 FVHVRGTSAS KEAIKISCSD SSPCRNILLQ DIDLEPSNGD GFTESFCWEA YGSSSGQVYP 430 440 PPCLSDDTSF LEQSVQSGIT SAYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Patterns of expansion and expression divergence in the plant polygalacturonase gene family." Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E. Genome Biol. 7:R87.1-R87.14(2006) [PubMed: 17010199] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana." Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A. J. Exp. Bot. 58:3719-3730(2007) [PubMed: 17928369] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009322 Genomic DNA. Translation: AAD55489.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE36366.1. AY046002 mRNA. Translation: AAK76676.1. AY142668 mRNA. Translation: AAN13206.1.
IPI	IPI00518842.
PIR	D96833.
RefSeq	NP_565232.1. NM_106666.3.
UniGene	At.11620.
3D structure databases
ProteinModelPortal	Q94AJ5.
SMR	Q94AJ5. Positions 63-415.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94AJ5. 2 interactions.
STRING	Q94AJ5.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	Q94AJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G80170.1; AT1G80170.1; AT1G80170.
GeneID	844357.
GenomeReviews	Gene locus AT1G80170 in contig CT485782_GR.
KEGG	ath:AT1G80170.
NMPDR	fig|3702.1.peg.7602.
Organism-specific databases
GeneFarm	519. 2.
TAIR	At1g80170.
Phylogenomic databases
eggNOG	COG5434.
GeneTree	EPGT00050000001253.
InParanoid	Q94AJ5.
OMA	KWLYFHG.
PhylomeDB	Q94AJ5.
ProtClustDB	PLN02793.
Gene expression databases
ArrayExpress	Q94AJ5.
Genevestigator	Q94AJ5.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR5_ARATH

Accession

Primary (citable) accession number: Q94AJ5
Secondary accession number(s): Q9SSC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program