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Protein

Mediator of RNA polymerase II transcription subunit 36a

Gene

MED36A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.2 Publications
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with RNAs of different lengths and types including dsRNAs and ssRNAs, rRNA, snoRNA, snRNA, siRNA and viral RNAs (PubMed:10829025). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-glutamine-[histone] = S-adenosyl-L-homocysteine + N5-methyl-L-glutamine-[histone].

GO - Molecular functioni

  • histone-glutamine methyltransferase activity Source: GO_Central
  • RNA binding Source: TAIR
  • rRNA methyltransferase activity Source: GO_Central
  • snoRNA binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Ribonucleoprotein, RNA-binding, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-ATH-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 36a
Alternative name(s):
Fibrillarin-like protein 2
Histone-glutamine methyltransferase
rRNA 2'-O-methyltransferase fibrillarin 2 (EC:2.1.1.-)
Gene namesi
Name:MED36A
Synonyms:FIB2, FLP, MED36_1
Ordered Locus Names:At4g25630
ORF Names:L73G19.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G25630.
TAIRilocus:2131386. AT4G25630.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi180 – 187GCVYAVEF → AAAAAAAA: No effect on RNA binding. 1 Publication8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485191 – 320Mediator of RNA polymerase II transcription subunit 36aAdd BLAST320

Post-translational modificationi

Methylated by PRMT11 and PRMT12.

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ94AH9.
PRIDEiQ94AH9.

PTM databases

SwissPalmiQ94AH9.

Expressioni

Tissue specificityi

Ubiquitously expressed, with higher levels in roots and flowers, and lower levels in siliques. Expression levels decrease during aging.2 Publications

Inductioni

Repressed by abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiQ94AH9. baseline and differential.
GenevisibleiQ94AH9. AT.

Interactioni

Subunit structurei

Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles (By similarity). Interacts with groundnut rosette virus long-distance movement protein; this interaction is required for virus long-distance movement protein transiting through host Cajal body and nucleolus, relocalization of fibrillarin to the cytoplasm, and in presence of viral RNA, leads to the formation of stable RNPs. Interacts (via GAR domain) with the hordeivirus TGB1 movement protein (via the first 82 amino acid residues). Component of the Mediator complex. Interacts with PRMT11 and PRMT12.By similarity6 Publications

Protein-protein interaction databases

BioGridi13955. 6 interactors.
DIPiDIP-39086N.
IntActiQ94AH9. 6 interactors.
STRINGi3702.AT4G25630.1.

Structurei

3D structure databases

ProteinModelPortaliQ94AH9.
SMRiQ94AH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 168S-adenosyl-L-methionine bindingBy similarity2
Regioni186 – 187S-adenosyl-L-methionine bindingBy similarity2
Regioni211 – 212S-adenosyl-L-methionine bindingBy similarity2
Regioni231 – 234S-adenosyl-L-methionine bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 75DMA/Gly-richAdd BLAST75

Domaini

The DMA/Gly-rich region (GAR domain) is rich in Gly and Arg and appear to function in nucleolar targeting (By similarity). The central (138-179) and C-terminal (225-281) part of the protein exhibit cooperative RNA-binding activities.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1596. Eukaryota.
COG1889. LUCA.
HOGENOMiHOG000106741.
InParanoidiQ94AH9.
KOiK14563.
OMAiQPNQAEI.
OrthoDBiEOG09360LL2.
PhylomeDBiQ94AH9.

Family and domain databases

HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiView protein in InterPro
IPR000692. Fibrillarin.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01269. Fibrillarin. 1 hit.
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiView protein in SMART
SM01206. Fibrillarin. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94AH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPLTGSGG GFSGGRGRGG YSGGRGDGGF SGGRGGGGRG GGRGFSDRGG
60 70 80 90 100
RGRGRGPPRG GARGGRGPAG RGGMKGGSKV IVEPHRHAGV FIAKGKEDAL
110 120 130 140 150
VTKNLVPGEA VYNEKRISVQ NEDGTKTEYR VWNPFRSKLA AAILGGVDNI
160 170 180 190 200
WIKPGAKVLY LGAASGTTVS HVSDLVGPEG CVYAVEFSHR SGRDLVNMAK
210 220 230 240 250
KRTNVIPIIE DARHPAKYRM LVGMVDVIFS DVAQPDQARI LALNASYFLK
260 270 280 290 300
SGGHFVISIK ANCIDSTVPA EAVFQTEVKK LQQEQFKPAE QVTLEPFERD
310 320
HACVVGGYRM PKKPKAATAA
Length:320
Mass (Da):33,653
Last modified:June 7, 2005 - v2
Checksum:i0E3DF1733F52CFDC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181C → S in AAK76701 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233444 mRNA. Translation: AAG10104.1.
AF267171 Genomic DNA. Translation: AAG10153.1.
AL050400 Genomic DNA. Translation: CAB43694.1.
AL161563 Genomic DNA. Translation: CAB81373.1.
CP002687 Genomic DNA. Translation: AEE85085.1.
AY046027 mRNA. Translation: AAK76701.1.
AY142647 mRNA. Translation: AAN13105.1.
AY084608 mRNA. Translation: AAM61172.1.
PIRiT09555.
RefSeqiNP_567724.1. NM_118695.4.
UniGeneiAt.25589.

Genome annotation databases

EnsemblPlantsiAT4G25630.1; AT4G25630.1; AT4G25630.
GeneIDi828668.
GrameneiAT4G25630.1; AT4G25630.1; AT4G25630.
KEGGiath:AT4G25630.

Similar proteinsi

Entry informationi

Entry nameiMD36A_ARATH
AccessioniPrimary (citable) accession number: Q94AH9
Secondary accession number(s): Q9SZZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: October 25, 2017
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families