Q94AH8 (TPS6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase 6
Short name=AtTPS6

Gene names

Name:	TPS6
Ordered Locus Names:	At1g68020
ORF Names:	T23K23.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates plant architecture, shape of epidermal pavement cells and branching of trichomes. Ref.6
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Subunit structure	Binds to the phosphopeptide-binding site of GRF/14-3-3. Ref.5
Tissue specificity	Expressed in seedlings, leaves, stems, flowers, siliques and roots. Ref.6
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family. In the C-terminal section; belongs to the trehalose phosphatase family.
Sequence caution	The sequence AAG52003.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from genetic interaction Ref.6. Source: TAIR
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from genetic interaction Ref.6. Source: TAIR trehalose-phosphatase activity Inferred from genetic interaction Ref.6. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q94AH8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: Q94AH8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     675-700: LRKAVEWENCVAAVDCSWKQIAEPVM → YSTKTFYFLALPLYLITQAPSNYYTG
     701-860: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 860	860	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6		PRO_0000324827
Regions
	Region	53 – 557	505	Glycosyltransferase
	Compositional bias	75 – 81	7	Poly-Ser
Amino acid modifications
	Modified residue	589	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	675 – 700	26	LRKAV…AEPVM → YSTKTFYFLALPLYLITQAP SNYYTG in isoform 2.		VSP_032376
	Alternative sequence	701 – 860	160	Missing in isoform 2.		VSP_032377
Experimental info
	Mutagenesis	152	1	R → S in cps-1; loss of pavement cell lobes and altered trichomes branching. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: A155BEB4EDAFD3A9	FASTA	860	97,703
	10 20 30 40 50 60 MVSRSYSNLL ELASGDSPTF GRMNRQIPRI MAVAGIMSNI DNDSKDTDLS PKDRIIIVAN 70 80 90 100 110 120 ELPIRAQRRV DGNGSGSSSS STCCSKGWNF SWDENSLLLQ LKDGLGDEAI EVIYVGCLKE 130 140 150 160 170 180 EIPLNEQEEV YQILLESFKC VPTFLPLDLY TRYYHGFCKQ QLWPLFHYML PLSPDLGGRF 190 200 210 220 230 240 DRTLWQAYVS VNKIFADRIM EVINPEDDFV WIHDYHLMVL PTFLRKRFNR VKLGFFLHSP 250 260 270 280 290 300 FPSSEIYKTL PIREELLRAL LNSDLIGFHT FDYARHFLSC CSRMLGLTYE SKRGYIGLEY 310 320 330 340 350 360 YGRTVSIKIL PVGIHMGQLQ SVLSLPETER KVGELIERYG RKGRTMLLGV DDMDIFKGIT 370 380 390 400 410 420 LKLLAMEQLL MQHPEWQGKV VLVQIANPAR GKGKDVKEMQ AETYSTVKRI NETFGRPGYD 430 440 450 460 470 480 PIVLIDAPLK FYERVAYYVV AECCLVTAVR DGMNLIPYEY IVSRQGNEKL DKILKLEANN 490 500 510 520 530 540 RNKKSMLVVS EFIGCSPSLS GAIRVNPWNV DAVADAMDSA LEVAEPEKQL RHEKHYKYVS 550 560 570 580 590 600 THDVGYWARS FLQDLERSCG EHGRRRCWGI GFGLSFRVVA LDQSFRKLSM EHIVSAYKRT 610 620 630 640 650 660 KTRAILLDYD DTLMPQGSID KRPSSKSIDI LNTLCRDKGN LVFIVSAKSR ETLSDWFSPC 670 680 690 700 710 720 EKLGIAAEHG YFLRLRKAVE WENCVAAVDC SWKQIAEPVM ELYTETTDGS TIEDKETALV 730 740 750 760 770 780 WSYEDADPDF GSCQAKELLD HLESVLANEP VTVKRGQNYV EVKPQGVSKG LIARRMLSMM 790 800 810 820 830 840 QERGTLPEFV LCIGDDRSDE DMFEVICSST EGPSIAPRAE IFACTVGQKP SKAKYYLDDT 850 860 TEIVRLMHGL ASVTDQITPV
	Isoform 2 [UniParc]. Checksum: DE323A4B10FCD3FE	FASTA	700	80,069
	10 20 30 40 50 60 MVSRSYSNLL ELASGDSPTF GRMNRQIPRI MAVAGIMSNI DNDSKDTDLS PKDRIIIVAN 70 80 90 100 110 120 ELPIRAQRRV DGNGSGSSSS STCCSKGWNF SWDENSLLLQ LKDGLGDEAI EVIYVGCLKE 130 140 150 160 170 180 EIPLNEQEEV YQILLESFKC VPTFLPLDLY TRYYHGFCKQ QLWPLFHYML PLSPDLGGRF 190 200 210 220 230 240 DRTLWQAYVS VNKIFADRIM EVINPEDDFV WIHDYHLMVL PTFLRKRFNR VKLGFFLHSP 250 260 270 280 290 300 FPSSEIYKTL PIREELLRAL LNSDLIGFHT FDYARHFLSC CSRMLGLTYE SKRGYIGLEY 310 320 330 340 350 360 YGRTVSIKIL PVGIHMGQLQ SVLSLPETER KVGELIERYG RKGRTMLLGV DDMDIFKGIT 370 380 390 400 410 420 LKLLAMEQLL MQHPEWQGKV VLVQIANPAR GKGKDVKEMQ AETYSTVKRI NETFGRPGYD 430 440 450 460 470 480 PIVLIDAPLK FYERVAYYVV AECCLVTAVR DGMNLIPYEY IVSRQGNEKL DKILKLEANN 490 500 510 520 530 540 RNKKSMLVVS EFIGCSPSLS GAIRVNPWNV DAVADAMDSA LEVAEPEKQL RHEKHYKYVS 550 560 570 580 590 600 THDVGYWARS FLQDLERSCG EHGRRRCWGI GFGLSFRVVA LDQSFRKLSM EHIVSAYKRT 610 620 630 640 650 660 KTRAILLDYD DTLMPQGSID KRPSSKSIDI LNTLCRDKGN LVFIVSAKSR ETLSDWFSPC 670 680 690 700 EKLGIAAEHG YFLRYSTKTF YFLALPLYLI TQAPSNYYTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM2). Strain: cv. Columbia.
[4]	"An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana." Leyman B., Van Dijck P., Thevelein J.M. Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose." Harthill J.E., Meek S.E.M., Morrice N., Peggie M.W., Borch J., Wong B.H.C., Mackintosh C. Plant J. 47:211-223(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, INTERACTION WITH GRF/14-3-3, MASS SPECTROMETRY.
[6]	"Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis." Chary S.N., Hicks G.R., Choi Y.G., Carter D., Raikhel N.V. Plant Physiol. 146:97-107(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-152.
[7]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-589, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC012563 Genomic DNA. Translation: AAG52003.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE34737.1. CP002684 Genomic DNA. Translation: AEE34738.1. AY046028 mRNA. Translation: AAK76702.1.
IPI	IPI00516798. IPI00538850.
PIR	C96703.
RefSeq	NP_564918.1. NM_105472.3. NP_974105.1. NM_202376.2.
UniGene	At.19364.
3D structure databases
HSSP	HSSP built from PDB template 1GZ5 based on UniProtKB P31677.
ProteinModelPortal	Q94AH8.
SMR	Q94AH8. Positions 153-557.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G68020.2-P.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Proteomic databases
PaxDb	Q94AH8.
PRIDE	Q94AH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G68020.2; AT1G68020.2; AT1G68020.
GeneID	843130.
KEGG	ath:AT1G68020.
Organism-specific databases
TAIR	At1g68020.
Phylogenomic databases
eggNOG	COG0380.
HOGENOM	HOG000191476.
InParanoid	Q94AH8.
KO	K16055.
OMA	LMQHPEW.
PhylomeDB	Q94AH8.
ProtClustDB	PLN02205.
Gene expression databases
Genevestigator	Q94AH8.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. PF02358. Trehalose_PPase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit. TIGR00685. T6PP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPS6_ARATH

Accession

Primary (citable) accession number: Q94AH8
Secondary accession number(s): Q9C9W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program