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Q94AH8

- TPS6_ARATH

UniProt

Q94AH8 - TPS6_ARATH

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6

Gene

TPS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Regulates plant architecture, shape of epidermal pavement cells and branching of trichomes.1 Publication

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: TAIR
    2. trehalose-phosphatase activity Source: TAIR

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. trehalose biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:GQT-2365-MONOMER.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6 (EC:2.4.1.15)
    Alternative name(s):
    Trehalose-6-phosphate synthase 6
    Short name:
    AtTPS6
    Gene namesi
    Name:TPS6
    Ordered Locus Names:At1g68020
    ORF Names:T23K23.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G68020.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi152 – 1521R → S in cps-1; loss of pavement cell lobes and altered trichomes branching. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 860860Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6PRO_0000324827Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ94AH8.
    PRIDEiQ94AH8.

    Expressioni

    Tissue specificityi

    Expressed in seedlings, leaves, stems, flowers, siliques and roots.1 Publication

    Gene expression databases

    GenevestigatoriQ94AH8.

    Interactioni

    Subunit structurei

    Binds to the phosphopeptide-binding site of GRF/14-3-3.

    Protein-protein interaction databases

    BioGridi28351. 1 interaction.
    STRINGi3702.AT1G68020.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94AH8.
    SMRiQ94AH8. Positions 153-557, 600-657.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 557505GlycosyltransferaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 817Poly-Ser

    Sequence similaritiesi

    In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
    In the C-terminal section; belongs to the trehalose phosphatase family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191476.
    InParanoidiQ94AH8.
    KOiK16055.
    OMAiKETAIVW.
    PhylomeDBiQ94AH8.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR001830. Glyco_trans_20.
    IPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    PF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q94AH8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSRSYSNLL ELASGDSPTF GRMNRQIPRI MAVAGIMSNI DNDSKDTDLS    50
    PKDRIIIVAN ELPIRAQRRV DGNGSGSSSS STCCSKGWNF SWDENSLLLQ 100
    LKDGLGDEAI EVIYVGCLKE EIPLNEQEEV YQILLESFKC VPTFLPLDLY 150
    TRYYHGFCKQ QLWPLFHYML PLSPDLGGRF DRTLWQAYVS VNKIFADRIM 200
    EVINPEDDFV WIHDYHLMVL PTFLRKRFNR VKLGFFLHSP FPSSEIYKTL 250
    PIREELLRAL LNSDLIGFHT FDYARHFLSC CSRMLGLTYE SKRGYIGLEY 300
    YGRTVSIKIL PVGIHMGQLQ SVLSLPETER KVGELIERYG RKGRTMLLGV 350
    DDMDIFKGIT LKLLAMEQLL MQHPEWQGKV VLVQIANPAR GKGKDVKEMQ 400
    AETYSTVKRI NETFGRPGYD PIVLIDAPLK FYERVAYYVV AECCLVTAVR 450
    DGMNLIPYEY IVSRQGNEKL DKILKLEANN RNKKSMLVVS EFIGCSPSLS 500
    GAIRVNPWNV DAVADAMDSA LEVAEPEKQL RHEKHYKYVS THDVGYWARS 550
    FLQDLERSCG EHGRRRCWGI GFGLSFRVVA LDQSFRKLSM EHIVSAYKRT 600
    KTRAILLDYD DTLMPQGSID KRPSSKSIDI LNTLCRDKGN LVFIVSAKSR 650
    ETLSDWFSPC EKLGIAAEHG YFLRLRKAVE WENCVAAVDC SWKQIAEPVM 700
    ELYTETTDGS TIEDKETALV WSYEDADPDF GSCQAKELLD HLESVLANEP 750
    VTVKRGQNYV EVKPQGVSKG LIARRMLSMM QERGTLPEFV LCIGDDRSDE 800
    DMFEVICSST EGPSIAPRAE IFACTVGQKP SKAKYYLDDT TEIVRLMHGL 850
    ASVTDQITPV 860

    Note: Derived from EST data. No experimental confirmation available.

    Length:860
    Mass (Da):97,703
    Last modified:March 18, 2008 - v2
    Checksum:iA155BEB4EDAFD3A9
    GO
    Isoform 2 (identifier: Q94AH8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         675-700: LRKAVEWENCVAAVDCSWKQIAEPVM → YSTKTFYFLALPLYLITQAPSNYYTG
         701-860: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:700
    Mass (Da):80,069
    Checksum:iDE323A4B10FCD3FE
    GO

    Sequence cautioni

    The sequence AAG52003.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei675 – 70026LRKAV…AEPVM → YSTKTFYFLALPLYLITQAP SNYYTG in isoform 2. CuratedVSP_032376Add
    BLAST
    Alternative sequencei701 – 860160Missing in isoform 2. CuratedVSP_032377Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012563 Genomic DNA. Translation: AAG52003.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34737.1.
    CP002684 Genomic DNA. Translation: AEE34738.1.
    AY046028 mRNA. Translation: AAK76702.1.
    PIRiC96703.
    RefSeqiNP_564918.1. NM_105472.3. [Q94AH8-2]
    NP_974105.1. NM_202376.2. [Q94AH8-1]
    UniGeneiAt.19364.

    Genome annotation databases

    EnsemblPlantsiAT1G68020.2; AT1G68020.2; AT1G68020. [Q94AH8-1]
    GeneIDi843130.
    KEGGiath:AT1G68020.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012563 Genomic DNA. Translation: AAG52003.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34737.1 .
    CP002684 Genomic DNA. Translation: AEE34738.1 .
    AY046028 mRNA. Translation: AAK76702.1 .
    PIRi C96703.
    RefSeqi NP_564918.1. NM_105472.3. [Q94AH8-2 ]
    NP_974105.1. NM_202376.2. [Q94AH8-1 ]
    UniGenei At.19364.

    3D structure databases

    ProteinModelPortali Q94AH8.
    SMRi Q94AH8. Positions 153-557, 600-657.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28351. 1 interaction.
    STRINGi 3702.AT1G68020.2-P.

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Proteomic databases

    PaxDbi Q94AH8.
    PRIDEi Q94AH8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G68020.2 ; AT1G68020.2 ; AT1G68020 . [Q94AH8-1 ]
    GeneIDi 843130.
    KEGGi ath:AT1G68020.

    Organism-specific databases

    TAIRi AT1G68020.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191476.
    InParanoidi Q94AH8.
    KOi K16055.
    OMAi KETAIVW.
    PhylomeDBi Q94AH8.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2365-MONOMER.

    Gene expression databases

    Genevestigatori Q94AH8.

    Family and domain databases

    Gene3Di 3.40.50.1000. 2 hits.
    InterProi IPR001830. Glyco_trans_20.
    IPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    PF02358. Trehalose_PPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM2).
      Strain: cv. Columbia.
    4. "An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana."
      Leyman B., Van Dijck P., Thevelein J.M.
      Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose."
      Harthill J.E., Meek S.E.M., Morrice N., Peggie M.W., Borch J., Wong B.H.C., Mackintosh C.
      Plant J. 47:211-223(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INTERACTION WITH GRF/14-3-3, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis."
      Chary S.N., Hicks G.R., Choi Y.G., Carter D., Raikhel N.V.
      Plant Physiol. 146:97-107(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-152.

    Entry informationi

    Entry nameiTPS6_ARATH
    AccessioniPrimary (citable) accession number: Q94AH8
    Secondary accession number(s): Q9C9W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3