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Q94AH8 (TPS6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6

EC=2.4.1.15
Alternative name(s):
Trehalose-6-phosphate synthase 6
Short name=AtTPS6
Gene names
Name:TPS6
Ordered Locus Names:At1g68020
ORF Names:T23K23.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates plant architecture, shape of epidermal pavement cells and branching of trichomes. Ref.6

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Subunit structure

Binds to the phosphopeptide-binding site of GRF/14-3-3. Ref.5

Tissue specificity

Expressed in seedlings, leaves, stems, flowers, siliques and roots. Ref.6

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 20 family.

In the C-terminal section; belongs to the trehalose phosphatase family.

Sequence caution

The sequence AAG52003.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q94AH8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Derived from EST data. No experimental confirmation available.
Isoform 2 (identifier: Q94AH8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     675-700: LRKAVEWENCVAAVDCSWKQIAEPVM → YSTKTFYFLALPLYLITQAPSNYYTG
     701-860: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6
PRO_0000324827

Regions

Region53 – 557505Glycosyltransferase
Compositional bias75 – 817Poly-Ser

Amino acid modifications

Modified residue51Phosphoserine By similarity

Natural variations

Alternative sequence675 – 70026LRKAV…AEPVM → YSTKTFYFLALPLYLITQAP SNYYTG in isoform 2.
VSP_032376
Alternative sequence701 – 860160Missing in isoform 2.
VSP_032377

Experimental info

Mutagenesis1521R → S in cps-1; loss of pavement cell lobes and altered trichomes branching. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: A155BEB4EDAFD3A9

FASTA86097,703
        10         20         30         40         50         60 
MVSRSYSNLL ELASGDSPTF GRMNRQIPRI MAVAGIMSNI DNDSKDTDLS PKDRIIIVAN 

        70         80         90        100        110        120 
ELPIRAQRRV DGNGSGSSSS STCCSKGWNF SWDENSLLLQ LKDGLGDEAI EVIYVGCLKE 

       130        140        150        160        170        180 
EIPLNEQEEV YQILLESFKC VPTFLPLDLY TRYYHGFCKQ QLWPLFHYML PLSPDLGGRF 

       190        200        210        220        230        240 
DRTLWQAYVS VNKIFADRIM EVINPEDDFV WIHDYHLMVL PTFLRKRFNR VKLGFFLHSP 

       250        260        270        280        290        300 
FPSSEIYKTL PIREELLRAL LNSDLIGFHT FDYARHFLSC CSRMLGLTYE SKRGYIGLEY 

       310        320        330        340        350        360 
YGRTVSIKIL PVGIHMGQLQ SVLSLPETER KVGELIERYG RKGRTMLLGV DDMDIFKGIT 

       370        380        390        400        410        420 
LKLLAMEQLL MQHPEWQGKV VLVQIANPAR GKGKDVKEMQ AETYSTVKRI NETFGRPGYD 

       430        440        450        460        470        480 
PIVLIDAPLK FYERVAYYVV AECCLVTAVR DGMNLIPYEY IVSRQGNEKL DKILKLEANN 

       490        500        510        520        530        540 
RNKKSMLVVS EFIGCSPSLS GAIRVNPWNV DAVADAMDSA LEVAEPEKQL RHEKHYKYVS 

       550        560        570        580        590        600 
THDVGYWARS FLQDLERSCG EHGRRRCWGI GFGLSFRVVA LDQSFRKLSM EHIVSAYKRT 

       610        620        630        640        650        660 
KTRAILLDYD DTLMPQGSID KRPSSKSIDI LNTLCRDKGN LVFIVSAKSR ETLSDWFSPC 

       670        680        690        700        710        720 
EKLGIAAEHG YFLRLRKAVE WENCVAAVDC SWKQIAEPVM ELYTETTDGS TIEDKETALV 

       730        740        750        760        770        780 
WSYEDADPDF GSCQAKELLD HLESVLANEP VTVKRGQNYV EVKPQGVSKG LIARRMLSMM 

       790        800        810        820        830        840 
QERGTLPEFV LCIGDDRSDE DMFEVICSST EGPSIAPRAE IFACTVGQKP SKAKYYLDDT 

       850        860 
TEIVRLMHGL ASVTDQITPV 

« Hide

Isoform 2 [UniParc].

Checksum: DE323A4B10FCD3FE
Show »

FASTA70080,069

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM2).
Strain: cv. Columbia.
[4]"An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana."
Leyman B., Van Dijck P., Thevelein J.M.
Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose."
Harthill J.E., Meek S.E.M., Morrice N., Peggie M.W., Borch J., Wong B.H.C., Mackintosh C.
Plant J. 47:211-223(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INTERACTION WITH GRF/14-3-3, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis."
Chary S.N., Hicks G.R., Choi Y.G., Carter D., Raikhel N.V.
Plant Physiol. 146:97-107(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-152.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC012563 Genomic DNA. Translation: AAG52003.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34737.1.
CP002684 Genomic DNA. Translation: AEE34738.1.
AY046028 mRNA. Translation: AAK76702.1.
PIRC96703.
RefSeqNP_564918.1. NM_105472.3. [Q94AH8-2]
NP_974105.1. NM_202376.2. [Q94AH8-1]
UniGeneAt.19364.

3D structure databases

ProteinModelPortalQ94AH8.
SMRQ94AH8. Positions 153-557, 600-657.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid28351. 1 interaction.
STRING3702.AT1G68020.2-P.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Proteomic databases

PaxDbQ94AH8.
PRIDEQ94AH8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G68020.2; AT1G68020.2; AT1G68020. [Q94AH8-1]
GeneID843130.
KEGGath:AT1G68020.

Organism-specific databases

TAIRAT1G68020.

Phylogenomic databases

eggNOGCOG0380.
HOGENOMHOG000191476.
InParanoidQ94AH8.
KOK16055.
OMAKETAIVW.
PhylomeDBQ94AH8.

Enzyme and pathway databases

BioCycARA:GQT-2365-MONOMER.

Gene expression databases

GenevestigatorQ94AH8.

Family and domain databases

Gene3D3.40.50.1000. 2 hits.
InterProIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPS6_ARATH
AccessionPrimary (citable) accession number: Q94AH8
Secondary accession number(s): Q9C9W6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: June 11, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names