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Protein

Cullin-1

Gene

CUL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Regulator of mitotic processes which plays a role during gametogenesis and embryogenesis. Together with SKP1, RBX1 and a F-box protein, it forms a SCF complex. The functional specificity of this complex depends of the type of F-box protein. SCF(UFO) is implicated in floral organ development. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1/ZTL), SCF(ADO2/LKP2), SCF(ADO3/FKF1) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • cell cycle Source: UniProtKB-KW
  • embryo development ending in seed dormancy Source: TAIR
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • jasmonic acid mediated signaling pathway Source: TAIR
  • leaf development Source: TAIR
  • phloem or xylem histogenesis Source: TAIR
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • regulation of circadian rhythm Source: TAIR
  • response to auxin Source: TAIR
  • response to jasmonic acid Source: TAIR

Keywordsi

Molecular functionDevelopmental protein
Biological processAuxin signaling pathway, Cell cycle, Ethylene signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-1
Gene namesi
Name:CUL1
Ordered Locus Names:At4g02570
ORF Names:T10P11.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02570.
TAIRilocus:2132377. AT4G02570.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi682K → M: No neddylation. 2 Publications1
Mutagenesisi712K → M: No change in neddylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198041 – 738Cullin-1Add BLAST738

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki682Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)1 Publication

Post-translational modificationi

Neddylated (rubylated). Deneddylation occurs upon interaction with the COP9 signalosome (CSN) complex.2 Publications

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ94AH6.
PRIDEiQ94AH6.

Expressioni

Tissue specificityi

Expressed constitutively in roots, seedlings, stems, leaves and flowers.1 Publication

Developmental stagei

Strong accumulation in embryos (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ94AH6. baseline and differential.
GenevisibleiQ94AH6. AT.

Interactioni

Subunit structurei

Part of E3 ubiquitin ligase SCF complexes such as SCF(TIR1) and SCF(COI1). SCF(TIR1) is composed of CUL1, SKP1 (SKP1A or SKP1B), RBX1 (RBX1A or RBX1B) and TIR1, while SCF(COI1) is composed of CUL1, SKP1, RBX1 and COI1. A SNF1-related protein kinase (KIN10 and KIN11) can also be part of these SCF complexes. SCF(TIR1) is able to interact with the COP9 signalosome (CSN) complex. Interacts directly with some F-box proteins such as DOR, SKIP14, FBW2/SKIP18, SKIP19/FBL20 and SKIP28/MEE11. Interacts with CAND1.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi10962. 47 interactors.
DIPiDIP-33346N.
IntActiQ94AH6. 36 interactors.
STRINGi3702.AT4G02570.1.

Structurei

3D structure databases

ProteinModelPortaliQ94AH6.
SMRiQ94AH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
HOGENOMiHOG000176712.
InParanoidiQ94AH6.
KOiK03347.
OMAiRCRIRIT.
OrthoDBiEOG093603S8.
PhylomeDBiQ94AH6.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiView protein in InterPro
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR036317. Cullin_homology_sf.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom_sf.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PfamiView protein in Pfam
PF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
SMARTiView protein in SMART
SM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiView protein in PROSITE
PS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94AH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERKTIDLEQ GWDYMQTGIT KLKRILEGLN EPAFDSEQYM MLYTTIYNMC
60 70 80 90 100
TQKPPHDYSQ QLYDKYREAF EEYINSTVLP ALREKHDEFM LRELFKRWSN
110 120 130 140 150
HKVMVRWLSR FFYYLDRYFI ARRSLPPLNE VGLTCFRDLV YNELHSKVKQ
160 170 180 190 200
AVIALVDKER EGEQIDRALL KNVLDIYVEI GMGQMERYEE DFESFMLQDT
210 220 230 240 250
SSYYSRKASS WIQEDSCPDY MLKSEECLKK ERERVAHYLH SSSEPKLVEK
260 270 280 290 300
VQHELLVVFA SQLLEKEHSG CRALLRDDKV DDLSRMYRLY HKILRGLEPV
310 320 330 340 350
ANIFKQHVTA EGNALVQQAE DTATNQVANT ASVQEQVLIR KVIELHDKYM
360 370 380 390 400
VYVTECFQNH TLFHKALKEA FEIFCNKTVA GSSSAELLAT FCDNILKKGG
410 420 430 440 450
SEKLSDEAIE DTLEKVVKLL AYISDKDLFA EFYRKKLARR LLFDRSANDD
460 470 480 490 500
HERSILTKLK QQCGGQFTSK MEGMVTDLTL ARENQNSFED YLGSNPAANP
510 520 530 540 550
GIDLTVTVLT TGFWPSYKSF DINLPSEMIK CVEVFKGFYE TKTKHRKLTW
560 570 580 590 600
IYSLGTCHIN GKFDQKAIEL IVSTYQAAVL LLFNTTDKLS YTEILAQLNL
610 620 630 640 650
SHEDLVRLLH SLSCAKYKIL LKEPNTKTVS QNDAFEFNSK FTDRMRRIKI
660 670 680 690 700
PLPPVDERKK VVEDVDKDRR YAIDAAIVRI MKSRKVLGHQ QLVSECVEQL
710 720 730
SRMFKPDIKA IKKRMEDLIT RDYLERDKEN PNMFRYLA
Length:738
Mass (Da):86,302
Last modified:December 1, 2001 - v1
Checksum:i45041357DE8DAD8C
GO

Sequence cautioni

The sequence AAC78267 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80750 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318017 mRNA. Translation: CAC85264.1.
AC002330 Genomic DNA. Translation: AAC78267.1. Sequence problems.
AL161494 Genomic DNA. Translation: CAB80750.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82194.1.
CP002687 Genomic DNA. Translation: AEE82195.1.
CP002687 Genomic DNA. Translation: AEE82196.1.
CP002687 Genomic DNA. Translation: AEE82197.1.
AY046030 mRNA. Translation: AAK76704.1.
AY133878 mRNA. Translation: AAM91812.1.
AK222216 mRNA. Translation: BAD95380.1.
PIRiT01092.
RefSeqiNP_001031575.1. NM_001036498.3.
NP_001031576.1. NM_001036499.1.
NP_001190661.1. NM_001203732.1.
NP_567243.1. NM_116491.3.
UniGeneiAt.24877.
At.67849.

Genome annotation databases

EnsemblPlantsiAT4G02570.1; AT4G02570.1; AT4G02570.
AT4G02570.2; AT4G02570.2; AT4G02570.
AT4G02570.3; AT4G02570.3; AT4G02570.
AT4G02570.4; AT4G02570.4; AT4G02570.
GeneIDi825648.
GrameneiAT4G02570.1; AT4G02570.1; AT4G02570.
AT4G02570.2; AT4G02570.2; AT4G02570.
AT4G02570.3; AT4G02570.3; AT4G02570.
AT4G02570.4; AT4G02570.4; AT4G02570.
KEGGiath:AT4G02570.

Similar proteinsi

Entry informationi

Entry nameiCUL1_ARATH
AccessioniPrimary (citable) accession number: Q94AH6
Secondary accession number(s): O22770, Q56W31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: November 22, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families