Reviewed,
UniProtKB/Swiss-Prot Q94AH6 (CUL1_ARATH)
Last modified
June 16, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cullin-1 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 738 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Regulator of mitotic processes which plays a role during gametogenesis and embryogenesis. Together with SKP1, RBX1 and a F-box protein, it forms a SCF complex. The functional specificity of this complex depends of the type of F-box protein. SCF(UFO) is implicated in floral organ development. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1/ZTL), SCF(ADO2/LKP2), SCF(ADO3/FKF1) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Ref.1 |
| Pathway | |
| Subunit structure | Part of E3 ubiquitin ligase SCF complexes such as SCF(TIR1) and SCF(COI1). SCF(TIR1) is composed of CUL1, SKP1 (SKP1A or SKP1B), RBX1 (RBX1A or RBX1B) and TIR1, while SCF(COI1) is composed of CUL1, SKP1, RBX1 and COI1. A SNF1-related protein kinase (KIN10 and KIN11) can also be part of these SCF complexes. SCF(TIR1) is able to interact with the COP9 signalosome (CSN) complex. Interacts directly with some F-box proteins such as DOR, SKIP14, FBW2/SKIP18, SKIP19/FBL20 and SKIP28/MEE11. |
| Subcellular location | Nucleus. Cytoplasm. Spindle. Cytoplasm › cytoskeleton › phragmoplast. Note: Mainly nuclear during interphase and preprophase, but also weakly cytoplasmic during interphase. Associated to mitotic spindle during metaphase, and to the phragmoplast during telophase. Ref.1 Ref.7 |
| Tissue specificity | Expressed constitutively in roots, seedlings, stems, leaves and flowers. Ref.1 |
| Developmental stage | Strong accumulation in embryos (at protein level). Ref.1 |
| Post-translational modification | Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. |
| Sequence similarities | Belongs to the cullin family. |
| Sequence caution | The sequence AAC78267.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80750.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| At2g02560 | Q8L5Y6 | 6 | EBI-532411,EBI-602912 | |
| At2g31470 | Q5BPS3 | 1 | EBI-532411,EBI-2042375 | |
| COI1 | O04197 | 2 | EBI-532411,EBI-401159 | |
| EBF1 | Q9SKK0 | 1 | EBI-532411,EBI-401198 | |
| EBF2 | Q708Y0 | 1 | EBI-532411,EBI-593623 | |
| GID2 | Q9STX3 | 1 | EBI-532411,EBI-619033 | |
| IAA7 | Q38825 | 4 | EBI-532411,EBI-602959 | |
| RBX1A | Q940X7 | 6 | EBI-532411,EBI-532404 | |
| RCE1 | Q9SDY5 | 1 | EBI-532411,EBI-595116 | |
| SKP1A | Q39255 | 4 | EBI-532411,EBI-532357 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 738 | 738 | Cullin-1 | PRO_0000119804 | |||||
Amino acid modifications | |||||||||
| Cross-link | 682 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8) Ref.6 Ref.10 | |||||||
Experimental info | |||||||||
| Mutagenesis | 682 | 1 | K → M: No neddylation. Ref.6 | ||||||
| Mutagenesis | 712 | 1 | K → M: No change in neddylation. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Null mutation of AtCUL1 causes arrest in early embryogenesis in Arabidopsis." Shen W.-H., Parmentier Y., Hellmann H., Lechner E., Dong A., Masson J., Granier F., Lepiniec L., Estelle M., Genschik P. Mol. Biol. Cell 13:1916-1928(2002) [PubMed: 12058059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
| [2] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 491-738. Strain: cv. Columbia. |
| [5] | "Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana." Gray W.M., del Pozo J.C., Walker L., Hobbie L., Risseeuw E., Banks T., Crosby W.L., Yang M., Ma H., Estelle M. Genes Dev. 13:1678-1691(1999) [PubMed: 10398681] [Abstract] Cited for: INTERACTION WITH TIR1, IDENTIFICATION IN A SCF(TIR1) COMPLEX. |
| [6] | "The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1." del Pozo J.C., Estelle M. Proc. Natl. Acad. Sci. U.S.A. 96:15342-15347(1999) [PubMed: 10611386] [Abstract] Cited for: NEDDYLATION AT LYS-682, MUTAGENESIS OF LYS-682 AND LYS-712. |
| [7] | "SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase." Farras R., Ferrando A., Jasik J., Kleinow T., Oekresz L., Tiburcio A., Salchert K., del Pozo C., Schell J., Koncz C. EMBO J. 20:2742-2756(2001) [PubMed: 11387208] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SKP1A; KIN10 AND KIN11. |
| [8] | "Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCF(TIR1) in mediating auxin response." Schwechheimer C., Serino G., Callis J., Crosby W.L., Lyapina S., Deshaies R.J., Gray W.M., Estelle M., Deng X.-W. Science 292:1379-1382(2001) [PubMed: 11337587] [Abstract] Cited for: INTERACTION WITH THE CSN COMPLEX. |
| [9] | "The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects." Lechner E., Xie D., Grava S., Pigaglio E., Planchais S., Murray J.A.H., Parmentier Y., Mutterer J., Dubreucq B., Shen W.-H., Genschik P. J. Biol. Chem. 277:50069-50080(2002) [PubMed: 12381738] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; RBX1A AND RBX1B. |
| [10] | "CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome." Wang X., Kang D., Feng S., Serino G., Schwechheimer C., Wei N. Mol. Biol. Cell 13:646-655(2002) [PubMed: 11854419] [Abstract] Cited for: NEDDYLATION, DENEDDYLATION BY THE CSN COMPLEX. |
| [11] | "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKIP14; FBW2/SKIP18; SKIP19/FBL20 AND SKIP28/MEE11. |
| [12] | "F-box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis." Zhang Y., Xu W., Li Z., Deng X.W., Wu W., Xue Y. Plant Physiol. 148:2121-2133(2008) [PubMed: 18835996] [Abstract] Cited for: INTERACTION WITH DOR. |
Web resources
| PlantsUBQ A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants |
Cross-references
Sequence databases | |
|---|---|
| AJ318017 mRNA. Translation: CAC85264.1. AC002330 Genomic DNA. Translation: AAC78267.1. Sequence problems. AL161494 Genomic DNA. Translation: CAB80750.1. Sequence problems. AY046030 mRNA. Translation: AAK76704.1. AY133878 mRNA. Translation: AAM91812.1. AK222216 mRNA. Translation: BAD95380.1. | |
| IPI | IPI00524614. |
| PIR | T01092. |
| RefSeq | NP_001031575.1. NP_001031576.1. NP_567243.1. |
| UniGene | At.24877 At.67849 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IUY based on UniProtKB Q9JLV5. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q94AH6. 35 interactions. |
Proteomic databases | |
| PRIDE | Q94AH6. |
Genome annotation databases | |
| GeneID | 825648. |
| GenomeReviews | Gene locus AT4G02570 in contig CT486007_GR. |
| KEGG | ath:AT4G02570. |
| NMPDR | fig|3702.1.peg.18072. |
Organism-specific databases | |
| TAIR | At4g02570. |
Phylogenomic databases | |
| OMA | Q94AH6. EDAASNK. |
Gene expression databases | |
| GermOnline | AT4G02570. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR016157. Cullin_CS. IPR016158. Cullin_homology. IPR001373. Cullin_N. IPR019559. Cullin_neddylation_domain. IPR011991. Wing_hlx_DNA_bd. [Graphical view] |
| Gene3D | G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| Pfam | PF00888. Cullin. 1 hit. PF10557. Cullin_Nedd8. 1 hit. [Graphical view] |
| SMART | SM00182. CULLIN. 1 hit. [Graphical view] |
| PROSITE | PS01256. CULLIN_1. 1 hit. PS50069. CULLIN_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CUL1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q94AH6 Secondary accession number(s): O22770, Q56W31 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


