Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-adenosylmethionine carrier 1, chloroplastic/mitochondrial

Gene

SAMC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transporter involved in exchange reactions through membranes. Has a low uniporter activity. Specifically mediates the transport of S-adenosylmethionine (SAM) and its closest analogs. Probably involved in the uptake of SAM in exchange for S-adenosylhomocysteine (SAHC), which is produced from SAM in the mitochondrial matrix and plastidial stroma by methyltransferase activities.2 Publications

Enzyme regulationi

Inhibited strongly by tannic acid, bromocresol purple, mercuric chloride, mersalyl, p-hydroxymercuribenzoate, S-adenosylhomocysteine, S-adenosylcysteine and adenosylornithine, and to a lesser extent by N-ethylmaleimide, bathophenanthroline and pyridoxal-5'-P.1 Publication

Kineticsi

  1. KM=95 µM for the SAM/SAM exchange1 Publication
  1. Vmax=1.2 mmol/min/g enzyme1 Publication

GO - Molecular functioni

  • S-adenosyl-L-methionine transmembrane transporter activity Source: TAIR

GO - Biological processi

  • chloroplast organization Source: TAIR
  • mitochondrial transport Source: GO_Central
  • S-adenosyl-L-methionine transport Source: TAIR

Keywordsi

Biological processTransport
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-ATH-425393 Transport of inorganic cations/anions and amino acids/oligopeptides

Protein family/group databases

TCDBi2.A.29.18.2 the mitochondrial carrier (mc) family

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine carrier 1, chloroplastic/mitochondrial
Alternative name(s):
S-adenosylmethionine transporter 1
Short name:
AtSAMT1
Gene namesi
Name:SAMC1
Synonyms:SAMT1
Ordered Locus Names:At4g39460
ORF Names:F23K16.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G39460
TAIRilocus:2122452 AT4G39460

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Transmembranei230 – 250HelicalSequence analysisAdd BLAST21
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Severely growth-retarded phenotype and reduced chlorophyll and plastoquinone contents. Unable to germinate when homozygous.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38Chloroplast and mitochondrion1 PublicationAdd BLAST38
ChainiPRO_000042477139 – 325S-adenosylmethionine carrier 1, chloroplastic/mitochondrialAdd BLAST287

Proteomic databases

PaxDbiQ94AG6

Expressioni

Tissue specificityi

Expressed in seedlings, cotyledons, leaves and flowers. Lower levels of expression in stems and roots. Not detected in senescent leaves, petals and pollen grains.2 Publications

Gene expression databases

ExpressionAtlasiQ94AG6 baseline and differential
GenevisibleiQ94AG6 AT

Interactioni

Protein-protein interaction databases

IntActiQ94AG6, 1 interactor
STRINGi3702.AT4G39460.1

Structurei

3D structure databases

ProteinModelPortaliQ94AG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati52 – 124Solcar 1Add BLAST73
Repeati133 – 215Solcar 2Add BLAST83
Repeati228 – 310Solcar 3Add BLAST83

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0768 Eukaryota
ENOG4110BII LUCA
HOGENOMiHOG000038810
InParanoidiQ94AG6
KOiK15111
OMAiLKTRVML
OrthoDBiEOG09360K34
PhylomeDBiQ94AG6

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SUPFAMiSSF103506 SSF103506, 1 hit
PROSITEiView protein in PROSITE
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94AG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLTLSVDV KSSSATSHDV SKRVMQSSQL KINKGFFASV NTQEDKPFDF
60 70 80 90 100
FRTLFEGFIA GGTAGVVVET ALYPIDTIKT RLQAARGGGK IVLKGLYSGL
110 120 130 140 150
AGNIAGVLPA SALFVGVYEP TKQKLLKTFP DHLSAVAHLT AGAIGGLAAS
160 170 180 190 200
LIRVPTEVVK QRMQTGQFTS APSAVRMIAS KEGFRGLYAG YRSFLLRDLP
210 220 230 240 250
FDAIQFCIYE QLCLGYKKAA RRELSDPENA LIGAFAGALT GAVTTPLDVI
260 270 280 290 300
KTRLMVQGSA KQYQGIVDCV QTIVREEGAP ALLKGIGPRV LWIGIGGSIF
310 320
FGVLESTKRT LAQRRPNTVK ETKEE
Length:325
Mass (Da):34,861
Last modified:December 1, 2001 - v1
Checksum:iBBA74F93939E6318
GO

Sequence cautioni

The sequence CAB44681 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80609 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ627908 mRNA Translation: CAF29517.1
AM260490 mRNA Translation: CAJ91123.1
AL078620 Genomic DNA Translation: CAB44681.1 Sequence problems.
AL161595 Genomic DNA Translation: CAB80609.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE87074.1
CP002687 Genomic DNA Translation: AEE87075.1
CP002687 Genomic DNA Translation: ANM66368.1
AY046052 mRNA Translation: AAK76726.1
AY079349 mRNA Translation: AAL85080.1
PIRiT09362
RefSeqiNP_001190968.1, NM_001204039.1
NP_001328265.1, NM_001342543.1
NP_568060.1, NM_120106.3
UniGeneiAt.21435

Genome annotation databases

EnsemblPlantsiAT4G39460.1; AT4G39460.1; AT4G39460
AT4G39460.2; AT4G39460.2; AT4G39460
AT4G39460.3; AT4G39460.3; AT4G39460
GeneIDi830101
GrameneiAT4G39460.1; AT4G39460.1; AT4G39460
AT4G39460.2; AT4G39460.2; AT4G39460
AT4G39460.3; AT4G39460.3; AT4G39460
KEGGiath:AT4G39460

Similar proteinsi

Entry informationi

Entry nameiSAMC1_ARATH
AccessioniPrimary (citable) accession number: Q94AG6
Secondary accession number(s): Q9SVB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 11, 2013
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health