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Protein

UDP-glycosyltransferase 76E12

Gene

UGT76E12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Possesses quercetin 3-O-glucosyltransferase and 7-O-glucosyltransferase activities in vitro.1 Publication

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei280 – 2801UDP-glucoseBy similarity

GO - Molecular functioni

  1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
  2. quercetin 3-O-glucosyltransferase activity Source: TAIR
  3. quercetin 7-O-glucosyltransferase activity Source: TAIR

GO - Biological processi

  1. flavonoid biosynthetic process Source: GO_Central
  2. flavonoid glucuronidation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 76E12 (EC:2.4.1.-, EC:2.4.1.91)
Gene namesi
Name:UGT76E12
Ordered Locus Names:At3g46660
ORF Names:F12A12.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G46660.

Subcellular locationi

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458UDP-glycosyltransferase 76E12PRO_0000409095Add
BLAST

Proteomic databases

PRIDEiQ94AB5.

Expressioni

Gene expression databases

ExpressionAtlasiQ94AB5. differential.
GenevestigatoriQ94AB5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G46660.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ94AB5.
SMRiQ94AB5. Positions 15-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni339 – 3413UDP-glucose bindingBy similarity
Regioni356 – 3649UDP-glucose bindingBy similarity
Regioni378 – 3814UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiNOG326467.
HOGENOMiHOG000237564.
InParanoidiQ94AB5.
OMAiITISHAH.
PhylomeDBiQ94AB5.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94AB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVLGMEEKP ARRSVVLVPF PAQGHISPMM QLAKTLHLKG FSITVVQTKF
60 70 80 90 100
NYFSPSDDFT HDFQFVTIPE SLPESDFKNL GPIQFLFKLN KECKVSFKDC
110 120 130 140 150
LGQLVLQQSN EISCVIYDEF MYFAEAAAKE CKLPNIIFST TSATAFACRS
160 170 180 190 200
VFDKLYANNV QAPLKETKGQ QEELVPEFYP LRYKDFPVSR FASLESIMEV
210 220 230 240 250
YRNTVDKRTA SSVIINTASC LESSSLSFLQ QQQLQIPVYP IGPLHMVASA
260 270 280 290 300
PTSLLEENKS CIEWLNKQKV NSVIYISMGS IALMEINEIM EVASGLAASN
310 320 330 340 350
QHFLWVIRPG SIPGSEWIES MPEEFSKMVL DRGYIVKWAP QKEVLSHPAV
360 370 380 390 400
GGFWSHCGWN STLESIGQGV PMICRPFSGD QKVNARYLEC VWKIGIQVEG
410 420 430 440 450
ELDRGVVERA VKRLMVDEEG EEMRKRAFSL KEQLRASVKS GGSSHNSLEE

FVHFIRTL
Length:458
Mass (Da):51,662
Last modified:December 1, 2001 - v1
Checksum:i064AC578C026B098
GO

Sequence cautioni

The sequence CAB62336.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133314 Genomic DNA. Translation: CAB62336.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE78190.1.
AY048297 mRNA. Translation: AAK82559.1.
AY120731 mRNA. Translation: AAM53289.1.
BT000356 mRNA. Translation: AAN15675.1.
BT002638 mRNA. Translation: AAO11554.1.
PIRiT45603.
RefSeqiNP_566885.1. NM_114533.2.
UniGeneiAt.600.

Genome annotation databases

EnsemblPlantsiAT3G46660.1; AT3G46660.1; AT3G46660.
GeneIDi823819.
KEGGiath:AT3G46660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133314 Genomic DNA. Translation: CAB62336.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE78190.1.
AY048297 mRNA. Translation: AAK82559.1.
AY120731 mRNA. Translation: AAM53289.1.
BT000356 mRNA. Translation: AAN15675.1.
BT002638 mRNA. Translation: AAO11554.1.
PIRiT45603.
RefSeqiNP_566885.1. NM_114533.2.
UniGeneiAt.600.

3D structure databases

ProteinModelPortaliQ94AB5.
SMRiQ94AB5. Positions 15-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G46660.1-P.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PRIDEiQ94AB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G46660.1; AT3G46660.1; AT3G46660.
GeneIDi823819.
KEGGiath:AT3G46660.

Organism-specific databases

TAIRiAT3G46660.

Phylogenomic databases

eggNOGiNOG326467.
HOGENOMiHOG000237564.
InParanoidiQ94AB5.
OMAiITISHAH.
PhylomeDBiQ94AB5.

Gene expression databases

ExpressionAtlasiQ94AB5. differential.
GenevestigatoriQ94AB5.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
    Li Y., Baldauf S., Lim E.K., Bowles D.J.
    J. Biol. Chem. 276:4338-4343(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  5. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
    Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
    Biotechnol. Bioeng. 87:623-631(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiU7E12_ARATH
AccessioniPrimary (citable) accession number: Q94AB5
Secondary accession number(s): Q9SNB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.