Q94AA9 (XGD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xylogalacturonan beta-1,3-xylosyltransferase
EC=2.4.2.41

Alternative name(s):
Protein XYLOGALACTURONAN DEFICIENT 1

Gene names

Name:	XGD1
Ordered Locus Names:	At5g33290
ORF Names:	F19N2.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pectin biosynthesis. Catalyzes the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. Ref.5
Catalytic activity	Transfers a xylosyl residue from UDP-D-xylose to a D-galactose residue in xylogalacturonan, forming a beta-1,3-D-xylosyl-D-galactose linkage. Ref.5
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Highly expressed in adult leaves. Lower levels in young leaves, stems and roots. Ref.5
Induction	Up-regulated by biotic and abiotic stresses and senescence. Down-regulated by cytokinin and nematodes or Agrobacterium infection. Ref.5
Disruption phenotype	Decreased cell wall xylose. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 47 family.
Sequence caution	The sequence AAK83577.1 differs from that shown. Reason: Intron retention. The sequence BX831739 differs from that shown. Reason: Frameshift at position 210.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pectin biosynthetic process Inferred from mutant phenotype Ref.5. Source: TAIR xylogalacturonan metabolic process Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-xylosyltransferase activity Inferred from direct assay Ref.5. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Xylogalacturonan beta-1,3-xylosyltransferase		PRO_0000392291
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 500	470	Lumenal Potential
	Compositional bias	47 – 108	62	Ser-rich
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	418	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	471 – 500	30	VLNRP…LRLGT → LIKLTNCGGPKKKKKKKKKK in xgd1-2; loss of activity. Ref.5
	Sequence conflict	59	1	N → D in BX831739. Ref.4
	Sequence conflict	102	1	K → E in BX831739. Ref.4
	Sequence conflict	110 – 111	2	KI → RV in BX831739. Ref.4
	Sequence conflict	210	1	D → E in BX831739. Ref.4
	Sequence conflict	284	1	V → E in AAL62020. Ref.3
	Sequence conflict	284	1	V → E in AAK83577. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q94AA9 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: CF7446BDE554CE0A	FASTA	500	56,600
	10 20 30 40 50 60 MAAPRSRRCS LSLLTLFSIT LILISVSLFV STKPANKPFL DYRNQFSISI SISSPLEQNT 70 80 90 100 110 120 TNTSFVSASP PLSPLGQSNT TNTILASSSS SSSFSDHQNQ NKSPSPTSKK IVIRKRSGLD 130 140 150 160 170 180 KIESDLAKAR AAIKKAASTQ NYVSSLYKNP AAFHQSHTEM MNRFKVWTYT EGEVPLFHDG 190 200 210 220 230 240 PVNDIYGIEG QFMDEMCVDG PKSRSRFRAD RPENAHVFFI PFSVAKVIHF VYKPITSVEG 250 260 270 280 290 300 FSRARLHRLI EDYVDVVATK HPYWNRSQGG DHFMVSCHDW APDVIDGNPK LFEKFIRGLC 310 320 330 340 350 360 NANTSEGFRP NVDVSIPEIY LPKGKLGPSF LGKSPRVRSI LAFFAGRSHG EIRKILFQHW 370 380 390 400 410 420 KEMDNEVQVY DRLPPGKDYT KTMGMSKFCL CPSGWEVASP REVEAIYAGC VPVIISDNYS 430 440 450 460 470 480 LPFSDVLNWD SFSIQIPVSR IKEIKTILQS VSLVRYLKMY KRVLEVKQHF VLNRPAKPYD 490 500 VMHMMLHSIW LRRLNLRLGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis." Jensen J.K., Sorensen S.O., Harholt J., Geshi N., Sakuragi Y., Moller I., Zandleven J., Bernal A.J., Jensen N.B., Sorensen C., Pauly M., Beldman G., Willats W.G., Scheller H.V. Plant Cell 20:1289-1302(2008) [PubMed: 18460606] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF 471-VAL--THR-500, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC051625 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED93892.1. AY049234 mRNA. Translation: AAK83577.1. Sequence problems. AY072629 mRNA. Translation: AAL62020.1. BX831739 mRNA. No translation available.
IPI	IPI00518752.
RefSeq	NP_198314.2. NM_122853.3.
UniGene	At.19950. At.74966.
3D structure databases
ProteinModelPortal	Q94AA9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q94AA9.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PRIDE	Q94AA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G33290.1; AT5G33290.1; AT5G33290.
GeneID	833302.
GenomeReviews	Gene locus AT5G33290 in contig BA000015_GR.
KEGG	ath:AT5G33290.
Organism-specific databases
TAIR	At5g33290.
Phylogenomic databases
eggNOG	KOG1021.
GeneTree	EPGT00070000028243.
HOGENOM	HBG744139.
InParanoid	Q94AA9.
OMA	FFAGRSH.
PhylomeDB	Q94AA9.
ProtClustDB	CLSN2918632.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G33290-MONOMER.
Gene expression databases
Genevestigator	Q94AA9.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XGD1_ARATH

Accession

Primary (citable) accession number: Q94AA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	March 23, 2010
Last modified:	December 14, 2011
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program