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Protein

Xylogalacturonan beta-1,3-xylosyltransferase

Gene

XGD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pectin biosynthesis. Catalyzes the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors.1 Publication

Catalytic activityi

Transfers a xylosyl residue from UDP-D-xylose to a D-galactose residue in xylogalacturonan, forming a beta-1,3-D-xylosyl-D-galactose linkage.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin biosynthetic process Source: TAIR
  • protein glycosylation Source: InterPro
  • xylogalacturonan metabolic process Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciMetaCyc:AT5G33290-MONOMER.
BRENDAi2.4.2.41. 399.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylogalacturonan beta-1,3-xylosyltransferase (EC:2.4.2.41)
Alternative name(s):
Protein XYLOGALACTURONAN DEFICIENT 1
Gene namesi
Name:XGD1
Ordered Locus Names:At5g33290
ORF Names:F19N2.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G33290.
TAIRilocus:2145924. AT5G33290.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini31 – 500LumenalSequence analysisAdd BLAST470

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Decreased cell wall xylose.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi471 – 500VLNRP…LRLGT → LIKLTNCGGPKKKKKKKKKK in xgd1-2; loss of activity. 1 PublicationAdd BLAST30

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003922911 – 500Xylogalacturonan beta-1,3-xylosyltransferaseAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi265N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi418N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ94AA9.

Expressioni

Tissue specificityi

Highly expressed in adult leaves. Lower levels in young leaves, stems and roots.1 Publication

Inductioni

Up-regulated by biotic and abiotic stresses and senescence. Down-regulated by cytokinin and nematodes or Agrobacterium infection.1 Publication

Gene expression databases

ExpressionAtlasiQ94AA9. baseline and differential.
GenevisibleiQ94AA9. AT.

Interactioni

Protein-protein interaction databases

BioGridi18560. 1 interactor.
STRINGi3702.AT5G33290.1.

Structurei

3D structure databases

ProteinModelPortaliQ94AA9.
SMRiQ94AA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi47 – 108Ser-richAdd BLAST62

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1021. Eukaryota.
ENOG410XTFH. LUCA.
HOGENOMiHOG000238847.
InParanoidiQ94AA9.
KOiK18789.
OMAiHTEMMNR.
OrthoDBiEOG093609C4.
PhylomeDBiQ94AA9.

Family and domain databases

InterProiView protein in InterPro
IPR004263. Exostosin.
PfamiView protein in Pfam
PF03016. Exostosin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94AA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPRSRRCS LSLLTLFSIT LILISVSLFV STKPANKPFL DYRNQFSISI
60 70 80 90 100
SISSPLEQNT TNTSFVSASP PLSPLGQSNT TNTILASSSS SSSFSDHQNQ
110 120 130 140 150
NKSPSPTSKK IVIRKRSGLD KIESDLAKAR AAIKKAASTQ NYVSSLYKNP
160 170 180 190 200
AAFHQSHTEM MNRFKVWTYT EGEVPLFHDG PVNDIYGIEG QFMDEMCVDG
210 220 230 240 250
PKSRSRFRAD RPENAHVFFI PFSVAKVIHF VYKPITSVEG FSRARLHRLI
260 270 280 290 300
EDYVDVVATK HPYWNRSQGG DHFMVSCHDW APDVIDGNPK LFEKFIRGLC
310 320 330 340 350
NANTSEGFRP NVDVSIPEIY LPKGKLGPSF LGKSPRVRSI LAFFAGRSHG
360 370 380 390 400
EIRKILFQHW KEMDNEVQVY DRLPPGKDYT KTMGMSKFCL CPSGWEVASP
410 420 430 440 450
REVEAIYAGC VPVIISDNYS LPFSDVLNWD SFSIQIPVSR IKEIKTILQS
460 470 480 490 500
VSLVRYLKMY KRVLEVKQHF VLNRPAKPYD VMHMMLHSIW LRRLNLRLGT
Length:500
Mass (Da):56,600
Last modified:March 23, 2010 - v2
Checksum:iCF7446BDE554CE0A
GO

Sequence cautioni

The sequence AAK83577 differs from that shown. Intron retention.Curated
The sequence BX831739 differs from that shown. Reason: Frameshift at position 210.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59N → D in BX831739 (PubMed:14993207).Curated1
Sequence conflicti102K → E in BX831739 (PubMed:14993207).Curated1
Sequence conflicti110 – 111KI → RV in BX831739 (PubMed:14993207).Curated2
Sequence conflicti210D → E in BX831739 (PubMed:14993207).Curated1
Sequence conflicti284V → E in AAL62020 (PubMed:14593172).Curated1
Sequence conflicti284V → E in AAK83577 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC051625 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93892.1.
AY049234 mRNA. Translation: AAK83577.1. Sequence problems.
AY072629 mRNA. Translation: AAL62020.1.
BX831739 mRNA. No translation available.
RefSeqiNP_198314.2. NM_122853.4.
UniGeneiAt.19950.
At.74966.

Genome annotation databases

EnsemblPlantsiAT5G33290.1; AT5G33290.1; AT5G33290.
GeneIDi833302.
GrameneiAT5G33290.1; AT5G33290.1; AT5G33290.
KEGGiath:AT5G33290.

Similar proteinsi

Entry informationi

Entry nameiXGD1_ARATH
AccessioniPrimary (citable) accession number: Q94AA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 23, 2010
Last modified: October 25, 2017
This is version 78 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families