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Q94AA4

- PFKA3_ARATH

UniProt

Q94AA4 - PFKA3_ARATH

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Protein
ATP-dependent 6-phosphofructokinase 3
Gene
PFK3, At4g26270, T25K17.80
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by AMP By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATP; via amide nitrogen By similarity
Metal bindingi190 – 1901Magnesium; catalytic By similarity
Sitei191 – 1911Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei220 – 2201Proton acceptor By similarity
Binding sitei319 – 3191Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1652ATP By similarity
Nucleotide bindingi189 – 1924ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
  4. root epidermal cell differentiation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G26270-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 3 (EC:2.7.1.11)
Short name:
ATP-PFK 3
Short name:
Phosphofructokinase 3
Alternative name(s):
Phosphohexokinase 3
Gene namesi
Name:PFK3
Ordered Locus Names:At4g26270
ORF Names:T25K17.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G26270.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
  2. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489ATP-dependent 6-phosphofructokinase 3UniRule annotation
PRO_0000330770Add
BLAST

Proteomic databases

PaxDbiQ94AA4.
PRIDEiQ94AA4.

Expressioni

Tissue specificityi

Mostly expressed in roots and flowers.1 Publication

Gene expression databases

GenevestigatoriQ94AA4.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi14020. 2 interactions.
IntActiQ94AA4. 5 interactions.
STRINGi3702.AT4G26270.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ94AA4.
SMRiQ94AA4. Positions 28-442.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2203Substrate binding By similarity
Regioni263 – 2653Substrate binding By similarity
Regioni375 – 3784Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ94AA4.
KOiK00850.
OMAiLRLIQGM.
PhylomeDBiQ94AA4.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q94AA4-1 [UniParc]FASTAAdd to Basket

« Hide

MSTVESSKPK IINGSCGYVL EDVPHLSDYL PGLPTYPNPL QDNPAYSVVK    50
QYFVDADDSV PQKIVVHKDG PRGIHFRRAG PRQKVYFESD EVHACIVTCG 100
GLCPGLNTVI REIVSSLSYM YGVKRILGID GGYRGFYAKN TVSLDSKVVN 150
DIHKRGGTIL GTSRGGHDTT KIVDSIQDRG INQVYIIGGD GTQRGASVIF 200
EEIRRRGLKV AVIGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA 250
ESIENGIGVV KLMGRYSGFI AMYATLASRD VDCCLIPESP FYLEGEGGLF 300
EYIEKRLKES GHMVLVIAEG AGQDLMSKSM ESMTLKDASG NKLLKDVGLW 350
LSQSIKDHFN QKKMVMNLKY IDPTYMIRAV PSNASDNVYC TLLAQSAVHG 400
AMAGYTGYIS GLVNGRQTYI PFYRITEKQN HVVITDRMWA RLLSSTNQPS 450
FLGPKDVFDN KEKPMSALLD DGNCNGVVDV PPVTKEITK 489
Length:489
Mass (Da):53,666
Last modified:December 1, 2001 - v1
Checksum:iAE2C1EA15B64F7E0
GO

Sequence cautioni

The sequence CAB38956.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB79482.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL049171 Genomic DNA. Translation: CAB38956.1. Sequence problems.
AL161564 Genomic DNA. Translation: CAB79482.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85178.1.
AY049245 mRNA. Translation: AAK83587.1.
AY090267 mRNA. Translation: AAL90928.1.
PIRiT06011.
RefSeqiNP_567742.1. NM_118760.2.
UniGeneiAt.2887.

Genome annotation databases

EnsemblPlantsiAT4G26270.1; AT4G26270.1; AT4G26270.
GeneIDi828733.
KEGGiath:AT4G26270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL049171 Genomic DNA. Translation: CAB38956.1 . Sequence problems.
AL161564 Genomic DNA. Translation: CAB79482.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE85178.1 .
AY049245 mRNA. Translation: AAK83587.1 .
AY090267 mRNA. Translation: AAL90928.1 .
PIRi T06011.
RefSeqi NP_567742.1. NM_118760.2.
UniGenei At.2887.

3D structure databases

ProteinModelPortali Q94AA4.
SMRi Q94AA4. Positions 28-442.
ModBasei Search...

Protein-protein interaction databases

BioGridi 14020. 2 interactions.
IntActi Q94AA4. 5 interactions.
STRINGi 3702.AT4G26270.1-P.

Proteomic databases

PaxDbi Q94AA4.
PRIDEi Q94AA4.

Protocols and materials databases

DNASUi 828733.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G26270.1 ; AT4G26270.1 ; AT4G26270 .
GeneIDi 828733.
KEGGi ath:AT4G26270.

Organism-specific databases

TAIRi AT4G26270.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000016186.
InParanoidi Q94AA4.
KOi K00850.
OMAi LRLIQGM.
PhylomeDBi Q94AA4.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci ARA:AT4G26270-MONOMER.

Gene expression databases

Genevestigatori Q94AA4.

Family and domain databases

HAMAPi MF_01981. Phosphofructokinase_II_X.
InterProi IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA3_ARATH
AccessioniPrimary (citable) accession number: Q94AA4
Secondary accession number(s): Q9STQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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