Q94AA4 (K6PF3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 3
EC=2.7.1.11

Alternative name(s):
Phosphofructokinase 3
Phosphohexokinase 3

Gene names

Name:	PFK3
Ordered Locus Names:	At4g26270
ORF Names:	T25K17.80

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subcellular location	Cytoplasm Ref.4.
Tissue specificity	Mostly expressed in roots and flowers. Ref.4
Sequence similarities	Belongs to the phosphofructokinase family.
Sequence caution	The sequence CAB38956.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79482.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from direct assay Ref.4. Source: TAIR root epidermal cell differentiation Inferred from mutant phenotype PubMed 23362208. Source: TAIR
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro cytosol Inferred from direct assay Ref.4. Source: TAIR
Molecular_function	6-phosphofructokinase activity Inferred from direct assay Ref.4. Source: TAIR ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	6-phosphofructokinase 3		PRO_0000330770
Regions
	Nucleotide binding	111 – 115	5	ATP By similarity
	Nucleotide binding	247 – 251	5	ATP By similarity
	Nucleotide binding	265 – 282	18	ATP By similarity
Sites
	Active site	220	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q94AA4 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AE2C1EA15B64F7E0	FASTA	489	53,666
	10 20 30 40 50 60 MSTVESSKPK IINGSCGYVL EDVPHLSDYL PGLPTYPNPL QDNPAYSVVK QYFVDADDSV 70 80 90 100 110 120 PQKIVVHKDG PRGIHFRRAG PRQKVYFESD EVHACIVTCG GLCPGLNTVI REIVSSLSYM 130 140 150 160 170 180 YGVKRILGID GGYRGFYAKN TVSLDSKVVN DIHKRGGTIL GTSRGGHDTT KIVDSIQDRG 190 200 210 220 230 240 INQVYIIGGD GTQRGASVIF EEIRRRGLKV AVIGIPKTID NDIPVIDKSF GFDTAVEEAQ 250 260 270 280 290 300 RAINAAHVEA ESIENGIGVV KLMGRYSGFI AMYATLASRD VDCCLIPESP FYLEGEGGLF 310 320 330 340 350 360 EYIEKRLKES GHMVLVIAEG AGQDLMSKSM ESMTLKDASG NKLLKDVGLW LSQSIKDHFN 370 380 390 400 410 420 QKKMVMNLKY IDPTYMIRAV PSNASDNVYC TLLAQSAVHG AMAGYTGYIS GLVNGRQTYI 430 440 450 460 470 480 PFYRITEKQN HVVITDRMWA RLLSSTNQPS FLGPKDVFDN KEKPMSALLD DGNCNGVVDV PPVTKEITK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana." Mustroph A., Sonnewald U., Biemelt S. FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL049171 Genomic DNA. Translation: CAB38956.1. Sequence problems. AL161564 Genomic DNA. Translation: CAB79482.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE85178.1. AY049245 mRNA. Translation: AAK83587.1. AY090267 mRNA. Translation: AAL90928.1.
IPI	IPI00545493.
PIR	T06011.
RefSeq	NP_567742.1. NM_118760.2.
UniGene	At.2887.
3D structure databases
HSSP	HSSP built from PDB template 1MTO based on UniProtKB P00512.
ProteinModelPortal	Q94AA4.
SMR	Q94AA4. Positions 28-442.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94AA4. 5 interactions.
STRING	3702.AT4G26270.1-P.
Proteomic databases
PaxDb	Q94AA4.
PRIDE	Q94AA4.
Protocols and materials databases
DNASU	828733.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G26270.1; AT4G26270.1; AT4G26270.
GeneID	828733.
KEGG	ath:AT4G26270.
Organism-specific databases
TAIR	At4g26270.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000016186.
InParanoid	Q94AA4.
KO	K00850.
OMA	DIVPIRQ.
PhylomeDB	Q94AA4.
ProtClustDB	PLN02564.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Gene expression databases
Genevestigator	Q94AA4.
Family and domain databases
InterPro	IPR022953. Phosphofructokinase. IPR000023. Phosphofructokinase_dom. IPR012004. PyroP-dep_PFruKinase_TP0108. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

K6PF3_ARATH

Accession

Primary (citable) accession number: Q94AA4
Secondary accession number(s): Q9STQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program