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Q94AA4

- PFKA3_ARATH

UniProt

Q94AA4 - PFKA3_ARATH

Protein

ATP-dependent 6-phosphofructokinase 3

Gene

PFK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011ATP; via amide nitrogenUniRule annotation
    Metal bindingi190 – 1901Magnesium; catalyticUniRule annotation
    Sitei191 – 1911Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei220 – 2201Proton acceptorUniRule annotation
    Binding sitei319 – 3191SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi164 – 1652ATPUniRule annotation
    Nucleotide bindingi189 – 1924ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: TAIR
    4. root epidermal cell differentiation Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G26270-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 3UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 3UniRule annotation
    Short name:
    Phosphofructokinase 3UniRule annotation
    Alternative name(s):
    Phosphohexokinase 3UniRule annotation
    Gene namesi
    Name:PFK3UniRule annotation
    Ordered Locus Names:At4g26270
    ORF Names:T25K17.80
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G26270.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro
    2. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 489489ATP-dependent 6-phosphofructokinase 3PRO_0000330770Add
    BLAST

    Proteomic databases

    PaxDbiQ94AA4.
    PRIDEiQ94AA4.

    Expressioni

    Tissue specificityi

    Mostly expressed in roots and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ94AA4.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    BioGridi14020. 2 interactions.
    IntActiQ94AA4. 5 interactions.
    STRINGi3702.AT4G26270.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94AA4.
    SMRiQ94AA4. Positions 28-442.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2203Substrate bindingUniRule annotation
    Regioni263 – 2653Substrate bindingUniRule annotation
    Regioni375 – 3784Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000016186.
    InParanoidiQ94AA4.
    KOiK00850.
    OMAiLRLIQGM.
    PhylomeDBiQ94AA4.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X.
    InterProiIPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q94AA4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTVESSKPK IINGSCGYVL EDVPHLSDYL PGLPTYPNPL QDNPAYSVVK    50
    QYFVDADDSV PQKIVVHKDG PRGIHFRRAG PRQKVYFESD EVHACIVTCG 100
    GLCPGLNTVI REIVSSLSYM YGVKRILGID GGYRGFYAKN TVSLDSKVVN 150
    DIHKRGGTIL GTSRGGHDTT KIVDSIQDRG INQVYIIGGD GTQRGASVIF 200
    EEIRRRGLKV AVIGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA 250
    ESIENGIGVV KLMGRYSGFI AMYATLASRD VDCCLIPESP FYLEGEGGLF 300
    EYIEKRLKES GHMVLVIAEG AGQDLMSKSM ESMTLKDASG NKLLKDVGLW 350
    LSQSIKDHFN QKKMVMNLKY IDPTYMIRAV PSNASDNVYC TLLAQSAVHG 400
    AMAGYTGYIS GLVNGRQTYI PFYRITEKQN HVVITDRMWA RLLSSTNQPS 450
    FLGPKDVFDN KEKPMSALLD DGNCNGVVDV PPVTKEITK 489
    Length:489
    Mass (Da):53,666
    Last modified:December 1, 2001 - v1
    Checksum:iAE2C1EA15B64F7E0
    GO

    Sequence cautioni

    The sequence CAB38956.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB79482.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL049171 Genomic DNA. Translation: CAB38956.1. Sequence problems.
    AL161564 Genomic DNA. Translation: CAB79482.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE85178.1.
    AY049245 mRNA. Translation: AAK83587.1.
    AY090267 mRNA. Translation: AAL90928.1.
    PIRiT06011.
    RefSeqiNP_567742.1. NM_118760.2.
    UniGeneiAt.2887.

    Genome annotation databases

    EnsemblPlantsiAT4G26270.1; AT4G26270.1; AT4G26270.
    GeneIDi828733.
    KEGGiath:AT4G26270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL049171 Genomic DNA. Translation: CAB38956.1 . Sequence problems.
    AL161564 Genomic DNA. Translation: CAB79482.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE85178.1 .
    AY049245 mRNA. Translation: AAK83587.1 .
    AY090267 mRNA. Translation: AAL90928.1 .
    PIRi T06011.
    RefSeqi NP_567742.1. NM_118760.2.
    UniGenei At.2887.

    3D structure databases

    ProteinModelPortali Q94AA4.
    SMRi Q94AA4. Positions 28-442.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 14020. 2 interactions.
    IntActi Q94AA4. 5 interactions.
    STRINGi 3702.AT4G26270.1-P.

    Proteomic databases

    PaxDbi Q94AA4.
    PRIDEi Q94AA4.

    Protocols and materials databases

    DNASUi 828733.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G26270.1 ; AT4G26270.1 ; AT4G26270 .
    GeneIDi 828733.
    KEGGi ath:AT4G26270.

    Organism-specific databases

    TAIRi AT4G26270.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000016186.
    InParanoidi Q94AA4.
    KOi K00850.
    OMAi LRLIQGM.
    PhylomeDBi Q94AA4.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci ARA:AT4G26270-MONOMER.

    Gene expression databases

    Genevestigatori Q94AA4.

    Family and domain databases

    HAMAPi MF_01981. Phosphofructokinase_II_X.
    InterProi IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
      Mustroph A., Sonnewald U., Biemelt S.
      FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPFKA3_ARATH
    AccessioniPrimary (citable) accession number: Q94AA4
    Secondary accession number(s): Q9STQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3