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Protein

ATP-dependent 6-phosphofructokinase 3

Gene

PFK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATP; via amide nitrogenUniRule annotation1
Metal bindingi190Magnesium; catalyticUniRule annotation1
Sitei191Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei220Proton acceptorUniRule annotation1
Binding sitei319SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 165ATPUniRule annotation2
Nucleotide bindingi189 – 192ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: TAIR
  • root epidermal cell differentiation Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G26270-MONOMER
BRENDAi2.7.1.11 399
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 3UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 3UniRule annotation
Short name:
Phosphofructokinase 3UniRule annotation
Alternative name(s):
Phosphohexokinase 3UniRule annotation
Gene namesi
Name:PFK3UniRule annotation
Ordered Locus Names:At4g26270
ORF Names:T25K17.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26270
TAIRilocus:2136849 AT4G26270

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003307701 – 489ATP-dependent 6-phosphofructokinase 3Add BLAST489

Proteomic databases

PaxDbiQ94AA4
PRIDEiQ94AA4

Expressioni

Tissue specificityi

Mostly expressed in roots and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ94AA4 baseline and differential
GenevisibleiQ94AA4 AT

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi14020, 2 interactors
IntActiQ94AA4, 5 interactors
STRINGi3702.AT4G26270.1

Structurei

3D structure databases

ProteinModelPortaliQ94AA4
SMRiQ94AA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 220Substrate bindingUniRule annotation3
Regioni263 – 265Substrate bindingUniRule annotation3
Regioni375 – 378Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000016186
InParanoidiQ94AA4
KOiK00850
OMAiIDQSFGF
OrthoDBiEOG093609PL
PhylomeDBiQ94AA4

Family and domain databases

HAMAPiMF_01981 Phosphofructokinase_II_X, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR012004 PyroP-dep_PFK_TP0108
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000534 PPi_PFK_TP0108, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

Q94AA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVESSKPK IINGSCGYVL EDVPHLSDYL PGLPTYPNPL QDNPAYSVVK
60 70 80 90 100
QYFVDADDSV PQKIVVHKDG PRGIHFRRAG PRQKVYFESD EVHACIVTCG
110 120 130 140 150
GLCPGLNTVI REIVSSLSYM YGVKRILGID GGYRGFYAKN TVSLDSKVVN
160 170 180 190 200
DIHKRGGTIL GTSRGGHDTT KIVDSIQDRG INQVYIIGGD GTQRGASVIF
210 220 230 240 250
EEIRRRGLKV AVIGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA
260 270 280 290 300
ESIENGIGVV KLMGRYSGFI AMYATLASRD VDCCLIPESP FYLEGEGGLF
310 320 330 340 350
EYIEKRLKES GHMVLVIAEG AGQDLMSKSM ESMTLKDASG NKLLKDVGLW
360 370 380 390 400
LSQSIKDHFN QKKMVMNLKY IDPTYMIRAV PSNASDNVYC TLLAQSAVHG
410 420 430 440 450
AMAGYTGYIS GLVNGRQTYI PFYRITEKQN HVVITDRMWA RLLSSTNQPS
460 470 480
FLGPKDVFDN KEKPMSALLD DGNCNGVVDV PPVTKEITK
Length:489
Mass (Da):53,666
Last modified:December 1, 2001 - v1
Checksum:iAE2C1EA15B64F7E0
GO

Sequence cautioni

The sequence CAB38956 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79482 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049171 Genomic DNA Translation: CAB38956.1 Sequence problems.
AL161564 Genomic DNA Translation: CAB79482.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE85178.1
AY049245 mRNA Translation: AAK83587.1
AY090267 mRNA Translation: AAL90928.1
PIRiT06011
RefSeqiNP_567742.1, NM_118760.3
UniGeneiAt.2887

Genome annotation databases

EnsemblPlantsiAT4G26270.1; AT4G26270.1; AT4G26270
GeneIDi828733
GrameneiAT4G26270.1; AT4G26270.1; AT4G26270
KEGGiath:AT4G26270

Similar proteinsi

Entry informationi

Entry nameiPFKA3_ARATH
AccessioniPrimary (citable) accession number: Q94AA4
Secondary accession number(s): Q9STQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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