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Protein

Ubiquitin-conjugating enzyme E2 35

Gene

UBC35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.3 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • postreplication repair Source: TAIR
  • protein K63-linked ubiquitination Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to iron ion Source: TAIR
  • root epidermal cell differentiation Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 35 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 35
Ubiquitin carrier protein 35
Gene namesi
Name:UBC35
Synonyms:UBC13A, UBG13A
Ordered Locus Names:At1g78870
ORF Names:F9K20.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G78870.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions or in phosphate-deficient plants. Unable to form branched root hairs in response to iron-deficient conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003452001 – 153Ubiquitin-conjugating enzyme E2 35Add BLAST153

Proteomic databases

PaxDbiQ94A97.
PRIDEiQ94A97.

Expressioni

Tissue specificityi

Ubiquitously expressed at low level. Mainly expressed in the vasculature.3 Publications

Inductioni

Not induced by salt, abscisic acid, mannitol, H2O2, low temperature, MMS or iron.2 Publications

Interactioni

Subunit structurei

Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2V2Q158193EBI-994120,EBI-714329From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi29443. 8 interactors.
IntActiQ94A97. 2 interactors.
STRINGi3702.AT1G78870.1.

Structurei

3D structure databases

ProteinModelPortaliQ94A97.
SMRiQ94A97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
HOGENOMiHOG000233455.
InParanoidiQ94A97.
KOiK10580.
OMAiISACPHE.
PhylomeDBiQ94A97.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q94A97-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE
60 70 80 90 100
GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA
110 120 130 140 150
LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVD TAKEWTRLYA

SGA
Length:153
Mass (Da):17,192
Last modified:December 1, 2001 - v1
Checksum:i66FDD14FA347A8D0
GO
Isoform 2 (identifier: Q94A97-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: G → GR

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:154
Mass (Da):17,348
Checksum:iE5616F62F3894F92
GO
Isoform 3 (identifier: Q94A97-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-112: SIQA → RYMS
     113-153: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
Show »
Length:112
Mass (Da):12,851
Checksum:i99FC71C2CB9C008F
GO

Sequence cautioni

The sequence AAC83026 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03492951G → GR in isoform 2. Curated1
Alternative sequenceiVSP_034930109 – 112SIQA → RYMS in isoform 3. Curated4
Alternative sequenceiVSP_034931113 – 153Missing in isoform 3. CuratedAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027048 mRNA. Translation: AAY44874.1.
AC005679 Genomic DNA. Translation: AAC83026.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36164.1.
CP002684 Genomic DNA. Translation: AEE36165.1.
CP002684 Genomic DNA. Translation: AEE36166.1.
AY049261 mRNA. Translation: AAK83603.1.
BT000544 mRNA. Translation: AAN18113.1.
AY085854 mRNA. Translation: AAM63067.1.
PIRiB96818.
RefSeqiNP_001031298.1. NM_001036221.3. [Q94A97-3]
NP_565192.1. NM_106535.4. [Q94A97-1]
UniGeneiAt.46456.

Genome annotation databases

EnsemblPlantsiAT1G78870.2; AT1G78870.2; AT1G78870. [Q94A97-1]
GeneIDi844224.
GrameneiAT1G78870.2; AT1G78870.2; AT1G78870.
KEGGiath:AT1G78870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027048 mRNA. Translation: AAY44874.1.
AC005679 Genomic DNA. Translation: AAC83026.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36164.1.
CP002684 Genomic DNA. Translation: AEE36165.1.
CP002684 Genomic DNA. Translation: AEE36166.1.
AY049261 mRNA. Translation: AAK83603.1.
BT000544 mRNA. Translation: AAN18113.1.
AY085854 mRNA. Translation: AAM63067.1.
PIRiB96818.
RefSeqiNP_001031298.1. NM_001036221.3. [Q94A97-3]
NP_565192.1. NM_106535.4. [Q94A97-1]
UniGeneiAt.46456.

3D structure databases

ProteinModelPortaliQ94A97.
SMRiQ94A97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29443. 8 interactors.
IntActiQ94A97. 2 interactors.
STRINGi3702.AT1G78870.1.

Proteomic databases

PaxDbiQ94A97.
PRIDEiQ94A97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G78870.2; AT1G78870.2; AT1G78870. [Q94A97-1]
GeneIDi844224.
GrameneiAT1G78870.2; AT1G78870.2; AT1G78870.
KEGGiath:AT1G78870.

Organism-specific databases

TAIRiAT1G78870.

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
HOGENOMiHOG000233455.
InParanoidiQ94A97.
KOiK10580.
OMAiISACPHE.
PhylomeDBiQ94A97.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ94A97.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC35_ARATH
AccessioniPrimary (citable) accession number: Q94A97
Secondary accession number(s): Q2V4C1, Q9ZVA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Partly functionally redundant with UBC36.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.