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Q94A97 (UBC35_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 35

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 35
Gene names
Name:UBC35
Synonyms:UBC13A, UBG13A
Ordered Locus Names:At1g78870
ORF Names:F9K20.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length153 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron. Ref.1 Ref.6 Ref.9

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D. Ref.6 Ref.7 Ref.8

Tissue specificity

Ubiquitously expressed at low level. Mainly expressed in the vasculature. Ref.1 Ref.6 Ref.9

Induction

Not induced by salt, abscisic acid, mannitol, H2O2, low temperature, MMS or iron. Ref.6 Ref.9

Disruption phenotype

No visible phenotype under normal growth conditions or in phosphate-deficient plants. Unable to form branched root hairs in response to iron-deficient conditions. Ref.9

Miscellaneous

Partly functionally redundant with UBC36.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence AAC83026.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

UBE2V2Q158193EBI-994120,EBI-714329From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q94A97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q94A97-2)

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: G → GR
Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.
Isoform 3 (identifier: Q94A97-3)

The sequence of this isoform differs from the canonical sequence as follows:
     109-112: SIQA → RYMS
     113-153: Missing.
Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 153153Ubiquitin-conjugating enzyme E2 35
PRO_0000345200

Sites

Active site891Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence511G → GR in isoform 2.
VSP_034929
Alternative sequence109 – 1124SIQA → RYMS in isoform 3.
VSP_034930
Alternative sequence113 – 15341Missing in isoform 3.
VSP_034931

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 66FDD14FA347A8D0

FASTA15317,192
        10         20         30         40         50         60 
MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE GGVFKLELFL 

        70         80         90        100        110        120 
PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA LQIRTVLLSI QALLSAPNPD 

       130        140        150 
DPLSENIAKH WKSNEAEAVD TAKEWTRLYA SGA 

« Hide

Isoform 2 [UniParc].

Checksum: E5616F62F3894F92
Show »

FASTA15417,348
Isoform 3 [UniParc].

Checksum: 99FC71C2CB9C008F
Show »

FASTA11212,851

References

« Hide 'large scale' references
[1]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants."
Wen R., Newton L., Li G., Wang H., Xiao W.
Plant Mol. Biol. 61:241-253(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH YEAST AND HUMAN MMS2, TISSUE SPECIFICITY, INDUCTION.
[7]"Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis."
Yin X.-J., Volk S., Ljung K., Mehlmer N., Dolezal K., Ditengou F., Hanano S., Davis S.J., Schmelzer E., Sandberg G., Teige M., Palme K., Pickart C., Bachmair A.
Plant Cell 19:1898-1911(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGLG2.
[8]"Arabidopsis UEV1D promotes lysine-63-linked polyubiquitination and is involved in DNA damage response."
Wen R., Torres-Acosta J.A., Pastushok L., Lai X., Pelzer L., Wang H., Xiao W.
Plant Cell 20:213-227(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UEV1A; UEV1B; UEV1C AND UEV1D.
[9]"A lysine-63-linked ubiquitin chain-forming conjugase, UBC13, promotes the developmental responses to iron deficiency in Arabidopsis roots."
Li W., Schmidt W.
Plant J. 62:330-343(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY IRON, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ027048 mRNA. Translation: AAY44874.1.
AC005679 Genomic DNA. Translation: AAC83026.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36164.1.
CP002684 Genomic DNA. Translation: AEE36165.1.
CP002684 Genomic DNA. Translation: AEE36166.1.
AY049261 mRNA. Translation: AAK83603.1.
BT000544 mRNA. Translation: AAN18113.1.
AY085854 mRNA. Translation: AAM63067.1.
PIRB96818.
RefSeqNP_001031298.1. NM_001036221.2.
NP_565192.1. NM_106535.3.
NP_849902.2. NM_179571.2.
UniGeneAt.46456.

3D structure databases

ProteinModelPortalQ94A97.
SMRQ94A97. Positions 6-150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid29443. 6 interactions.
IntActQ94A97. 2 interactions.

Proteomic databases

PaxDbQ94A97.
PRIDEQ94A97.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G78870.2; AT1G78870.2; AT1G78870. [Q94A97-1]
GeneID844224.
KEGGath:AT1G78870.

Organism-specific databases

TAIRAT1G78870.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233455.
InParanoidQ94A97.
KOK10580.
OMANERRAIQ.
PhylomeDBQ94A97.
ProtClustDBCLSN2687835.

Enzyme and pathway databases

BioCycARA:AT1G78870-MONOMER.
ARA:GQT-676-MONOMER.
ARA:GQT-677-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ94A97.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC35_ARATH
AccessionPrimary (citable) accession number: Q94A97
Secondary accession number(s): Q2V4C1, Q9ZVA6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names