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Protein

Ubiquitin-conjugating enzyme E2 35

Gene

UBC35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.3 Publications

Miscellaneous

Partly functionally redundant with UBC36.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • postreplication repair Source: TAIR
  • protein K63-linked ubiquitination Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to iron ion Source: TAIR
  • root epidermal cell differentiation Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-9020702 Interleukin-1 signaling
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 35 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 35
Ubiquitin carrier protein 35
Gene namesi
Name:UBC35
Synonyms:UBC13A, UBG13A
Ordered Locus Names:At1g78870
ORF Names:F9K20.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G78870
TAIRilocus:2037553 AT1G78870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions or in phosphate-deficient plants. Unable to form branched root hairs in response to iron-deficient conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003452001 – 153Ubiquitin-conjugating enzyme E2 35Add BLAST153

Proteomic databases

PaxDbiQ94A97
PRIDEiQ94A97

Expressioni

Tissue specificityi

Ubiquitously expressed at low level. Mainly expressed in the vasculature.3 Publications

Inductioni

Not induced by salt, abscisic acid, mannitol, H2O2, low temperature, MMS or iron.2 Publications

Gene expression databases

ExpressionAtlasiQ94A97 baseline and differential

Interactioni

Subunit structurei

Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2V2Q158193EBI-994120,EBI-714329From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi29443, 8 interactors
IntActiQ94A97, 2 interactors
STRINGi3702.AT1G78870.1

Structurei

3D structure databases

ProteinModelPortaliQ94A97
SMRiQ94A97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233455
InParanoidiQ94A97
KOiK10580
OMAiYFDVTIQ
PhylomeDBiQ94A97

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q94A97-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE
60 70 80 90 100
GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA
110 120 130 140 150
LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVD TAKEWTRLYA

SGA
Length:153
Mass (Da):17,192
Last modified:December 1, 2001 - v1
Checksum:i66FDD14FA347A8D0
GO
Isoform 2 (identifier: Q94A97-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: G → GR

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:154
Mass (Da):17,348
Checksum:iE5616F62F3894F92
GO
Isoform 3 (identifier: Q94A97-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-112: SIQA → RYMS
     113-153: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
Show »
Length:112
Mass (Da):12,851
Checksum:i99FC71C2CB9C008F
GO

Sequence cautioni

The sequence AAC83026 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03492951G → GR in isoform 2. Curated1
Alternative sequenceiVSP_034930109 – 112SIQA → RYMS in isoform 3. Curated4
Alternative sequenceiVSP_034931113 – 153Missing in isoform 3. CuratedAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027048 mRNA Translation: AAY44874.1
AC005679 Genomic DNA Translation: AAC83026.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE36165.1
CP002684 Genomic DNA Translation: AEE36166.1
AY049261 mRNA Translation: AAK83603.1
BT000544 mRNA Translation: AAN18113.1
AY085854 mRNA Translation: AAM63067.1
PIRiB96818
RefSeqiNP_001031298.1, NM_001036221.3 [Q94A97-3]
NP_565192.1, NM_106535.4 [Q94A97-1]
UniGeneiAt.46456

Genome annotation databases

EnsemblPlantsiAT1G78870.2; AT1G78870.2; AT1G78870 [Q94A97-1]
AT1G78870.3; AT1G78870.3; AT1G78870 [Q94A97-3]
GeneIDi844224
GrameneiAT1G78870.2; AT1G78870.2; AT1G78870 [Q94A97-1]
AT1G78870.3; AT1G78870.3; AT1G78870 [Q94A97-3]
KEGGiath:AT1G78870

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC35_ARATH
AccessioniPrimary (citable) accession number: Q94A97
Secondary accession number(s): Q2V4C1, Q9ZVA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health