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Q94A97

- UBC35_ARATH

UniProt

Q94A97 - UBC35_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 35

Gene

UBC35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.3 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. postreplication repair Source: TAIR
  2. protein ubiquitination Source: GOC
  3. response to cadmium ion Source: TAIR
  4. response to iron ion Source: TAIR
  5. root epidermal cell differentiation Source: TAIR
  6. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G78870-MONOMER.
ARA:GQT-676-MONOMER.
ARA:GQT-677-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 35 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 35
Gene namesi
Name:UBC35
Synonyms:UBC13A, UBG13A
Ordered Locus Names:At1g78870
ORF Names:F9K20.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G78870.

Subcellular locationi

GO - Cellular componenti

  1. UBC13-MMS2 complex Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions or in phosphate-deficient plants. Unable to form branched root hairs in response to iron-deficient conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Ubiquitin-conjugating enzyme E2 35PRO_0000345200Add
BLAST

Proteomic databases

PaxDbiQ94A97.
PRIDEiQ94A97.

Expressioni

Tissue specificityi

Ubiquitously expressed at low level. Mainly expressed in the vasculature.3 Publications

Inductioni

Not induced by salt, abscisic acid, mannitol, H2O2, low temperature, MMS or iron.2 Publications

Gene expression databases

GenevestigatoriQ94A97.

Interactioni

Subunit structurei

Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2V2Q158193EBI-994120,EBI-714329From a different organism.

Protein-protein interaction databases

BioGridi29443. 6 interactions.
IntActiQ94A97. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ94A97.
SMRiQ94A97. Positions 6-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ94A97.
KOiK10580.
OMAiYAGNKNE.
PhylomeDBiQ94A97.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q94A97-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE
60 70 80 90 100
GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA
110 120 130 140 150
LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVD TAKEWTRLYA

SGA
Length:153
Mass (Da):17,192
Last modified:December 1, 2001 - v1
Checksum:i66FDD14FA347A8D0
GO
Isoform 2 (identifier: Q94A97-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-51: G → GR

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:154
Mass (Da):17,348
Checksum:iE5616F62F3894F92
GO
Isoform 3 (identifier: Q94A97-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-112: SIQA → RYMS
     113-153: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Show »
Length:112
Mass (Da):12,851
Checksum:i99FC71C2CB9C008F
GO

Sequence cautioni

The sequence AAC83026.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei51 – 511G → GR in isoform 2. CuratedVSP_034929
Alternative sequencei109 – 1124SIQA → RYMS in isoform 3. CuratedVSP_034930
Alternative sequencei113 – 15341Missing in isoform 3. CuratedVSP_034931Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027048 mRNA. Translation: AAY44874.1.
AC005679 Genomic DNA. Translation: AAC83026.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36164.1.
CP002684 Genomic DNA. Translation: AEE36165.1.
CP002684 Genomic DNA. Translation: AEE36166.1.
AY049261 mRNA. Translation: AAK83603.1.
BT000544 mRNA. Translation: AAN18113.1.
AY085854 mRNA. Translation: AAM63067.1.
PIRiB96818.
RefSeqiNP_001031298.1. NM_001036221.2. [Q94A97-3]
NP_565192.1. NM_106535.3. [Q94A97-1]
NP_849902.2. NM_179571.2. [Q94A97-2]
UniGeneiAt.46456.

Genome annotation databases

EnsemblPlantsiAT1G78870.2; AT1G78870.2; AT1G78870. [Q94A97-1]
GeneIDi844224.
KEGGiath:AT1G78870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027048 mRNA. Translation: AAY44874.1 .
AC005679 Genomic DNA. Translation: AAC83026.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE36164.1 .
CP002684 Genomic DNA. Translation: AEE36165.1 .
CP002684 Genomic DNA. Translation: AEE36166.1 .
AY049261 mRNA. Translation: AAK83603.1 .
BT000544 mRNA. Translation: AAN18113.1 .
AY085854 mRNA. Translation: AAM63067.1 .
PIRi B96818.
RefSeqi NP_001031298.1. NM_001036221.2. [Q94A97-3 ]
NP_565192.1. NM_106535.3. [Q94A97-1 ]
NP_849902.2. NM_179571.2. [Q94A97-2 ]
UniGenei At.46456.

3D structure databases

ProteinModelPortali Q94A97.
SMRi Q94A97. Positions 6-150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 29443. 6 interactions.
IntActi Q94A97. 2 interactions.

Proteomic databases

PaxDbi Q94A97.
PRIDEi Q94A97.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G78870.2 ; AT1G78870.2 ; AT1G78870 . [Q94A97-1 ]
GeneIDi 844224.
KEGGi ath:AT1G78870.

Organism-specific databases

TAIRi AT1G78870.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
InParanoidi Q94A97.
KOi K10580.
OMAi YAGNKNE.
PhylomeDBi Q94A97.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT1G78870-MONOMER.
ARA:GQT-676-MONOMER.
ARA:GQT-677-MONOMER.

Gene expression databases

Genevestigatori Q94A97.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants."
    Wen R., Newton L., Li G., Wang H., Xiao W.
    Plant Mol. Biol. 61:241-253(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YEAST AND HUMAN MMS2, TISSUE SPECIFICITY, INDUCTION.
  7. Cited for: INTERACTION WITH RGLG2.
  8. "Arabidopsis UEV1D promotes lysine-63-linked polyubiquitination and is involved in DNA damage response."
    Wen R., Torres-Acosta J.A., Pastushok L., Lai X., Pelzer L., Wang H., Xiao W.
    Plant Cell 20:213-227(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UEV1A; UEV1B; UEV1C AND UEV1D.
  9. "A lysine-63-linked ubiquitin chain-forming conjugase, UBC13, promotes the developmental responses to iron deficiency in Arabidopsis roots."
    Li W., Schmidt W.
    Plant J. 62:330-343(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY IRON, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiUBC35_ARATH
AccessioniPrimary (citable) accession number: Q94A97
Secondary accession number(s): Q2V4C1, Q9ZVA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Partly functionally redundant with UBC36.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3