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Protein

Potassium channel GORK

Gene

GORK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major selective outward-rectifying potassium channel of the guard cell membrane. Involved in regulation of stomatal movements according to the water status. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. Conductance of the channel is modulated in a potassium-dependent fashion. May interact with the cytoskeleton or with regulatory proteins.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi386 – 508cNMPAdd BLAST123

GO - Molecular functioni

  • ion transmembrane transporter activity Source: TAIR
  • outward rectifier potassium channel activity Source: TAIR

GO - Biological processi

  • ion transport Source: TAIR
  • regulation of membrane potential Source: GO_Central
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Protein family/group databases

TCDBi1.A.1.4.4. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel GORK
Alternative name(s):
Guard cell outward rectifying K(+) channel
Short name:
AtGORK
Gene namesi
Name:GORK
Ordered Locus Names:At5g37500
ORF Names:MPA22.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G37500.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 69CytoplasmicSequence analysisAdd BLAST69
Transmembranei70 – 90Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini91 – 97ExtracellularSequence analysis7
Transmembranei98 – 118Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini119 – 141CytoplasmicSequence analysisAdd BLAST23
Transmembranei142 – 162Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini163 – 168ExtracellularSequence analysis6
Transmembranei169 – 189Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini190 – 203CytoplasmicSequence analysisAdd BLAST14
Transmembranei204 – 224Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini225 – 259ExtracellularSequence analysisAdd BLAST35
Intramembranei260 – 279Pore-forming; Name=Segment H5Sequence analysisAdd BLAST20
Topological domaini280 – 285ExtracellularSequence analysis6
Transmembranei286 – 306Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini307 – 820CytoplasmicSequence analysisAdd BLAST514

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541291 – 820Potassium channel GORKAdd BLAST820

Proteomic databases

PaxDbiQ94A76.

PTM databases

iPTMnetiQ94A76.

Expressioni

Tissue specificityi

Expressed in guard cell-containing tissues, in root epidermal cells and in root hairs. Detected in vascular cells of the root and shoot.2 Publications

Inductioni

Up-regulated under drought, salt stress and cold conditions. Induced by abscisic acid (ABA) treatment in roots and shoots but not in guard cells.1 Publication

Gene expression databases

GenevisibleiQ94A76. AT.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.By similarity

Protein-protein interaction databases

BioGridi18979. 3 interactors.
IntActiQ94A76. 2 interactors.
STRINGi3702.AT5G37500.1.

Structurei

3D structure databases

ProteinModelPortaliQ94A76.
SMRiQ94A76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati528 – 559ANK 1Add BLAST32
Repeati563 – 592ANK 2Add BLAST30
Repeati596 – 625ANK 3Add BLAST30
Repeati627 – 656ANK 4Add BLAST30
Repeati660 – 689ANK 5Add BLAST30
Repeati693 – 722ANK 6Add BLAST30
Domaini740 – 820KHAPROSITE-ProRule annotationAdd BLAST81

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 KHA domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000240830.
InParanoidiQ94A76.
OMAiRITDIDM.
OrthoDBiEOG093602RR.
PhylomeDBiQ94A76.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94A76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRLRRRQEI IDHEEEESND DVSSRRGKLS LAETFRWLDS SEHRRIETDG
60 70 80 90 100
HNDYKYIIHP KNRWYKAWEM FILVWAIYSS LFTPMEFGFF RGLPERLFVL
110 120 130 140 150
DIVGQIAFLV DIVLQFFVAY RDTQTYRTVY KPTRIAFRYL KSHFLMDFIG
160 170 180 190 200
CFPWDLIYKA SGKHELVRYL LWIRLFRVRK VVEFFQRLEK DTRINYLFTR
210 220 230 240 250
ILKLLFVEVY CTHTAACIFY YLATTLPPEN EGYTWIGSLK LGDYSYENFR
260 270 280 290 300
EIDLWKRYTT ALYFAIVTMA TVGYGDIHAV NLREMIFVMI YVSFDMVLGA
310 320 330 340 350
YLIGNITALI VKGSNTERFR DKMNDLISFM NRKKLGRDLR SQITGHVRLQ
360 370 380 390 400
YDSHYTDTVM LQDIPASIRA KIAQLLYLPY IKKVPLFKGC STEFINQIVI
410 420 430 440 450
RLHEEYFLPG EVITEQGNVV DHLYFVCEGL LEALVTKTDG SEESVTLLGP
460 470 480 490 500
HTSFGDISII CNISQPFTVR VCELCHLLRL DKQSFSNILE IYFHDGRTIL
510 520 530 540 550
NNIMEEKESN DRIKKLESDI VIHIGKQEAE LALKVNSAAF QGDFYQLKSL
560 570 580 590 600
IRSGADPNKT DYDGRSPLHL AACRGYEDIT LFLIQEGVDV NLKDKFGHTP
610 620 630 640 650
LFEAVKAGQE GVIGLLVKEG ASFNLEDSGN FLCTTVAKGD SDFLKRLLSS
660 670 680 690 700
GMNPNSEDYD HRTPLHVAAS EGLFLMAKML VEAGASVISK DRWGNSPLDE
710 720 730 740 750
ARLCGNKKLI KLLEDVKNAQ SSIYPSSLRE LQEERIERRK CTVFPFHPQE
760 770 780 790 800
AKEERSRKHG VVVWIPSNLE KLIVTAAKEL GLSDGASFVL LSEDQGRITD
810 820
IDMISDGHKL YMISDTTDQT
Length:820
Mass (Da):94,461
Last modified:March 1, 2004 - v2
Checksum:i65F57190056A9AE4
GO

Sequence cautioni

The sequence AAK83636 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125T → S in CAC17380 (PubMed:11113445).Curated1
Sequence conflicti230N → Q in CAC17380 (PubMed:11113445).Curated1
Sequence conflicti306I → V in CAC17380 (PubMed:11113445).Curated1
Sequence conflicti765I → V in CAC17380 (PubMed:11113445).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279009 mRNA. Translation: CAC17380.1.
AB025630 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED94198.1.
AY049294 mRNA. Translation: AAK83636.1. Different initiation.
AY143848 mRNA. Translation: AAN28787.1.
RefSeqiNP_198566.2. NM_123109.5.
UniGeneiAt.10193.

Genome annotation databases

EnsemblPlantsiAT5G37500.1; AT5G37500.1; AT5G37500.
GeneIDi833728.
GrameneiAT5G37500.1; AT5G37500.1; AT5G37500.
KEGGiath:AT5G37500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279009 mRNA. Translation: CAC17380.1.
AB025630 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED94198.1.
AY049294 mRNA. Translation: AAK83636.1. Different initiation.
AY143848 mRNA. Translation: AAN28787.1.
RefSeqiNP_198566.2. NM_123109.5.
UniGeneiAt.10193.

3D structure databases

ProteinModelPortaliQ94A76.
SMRiQ94A76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18979. 3 interactors.
IntActiQ94A76. 2 interactors.
STRINGi3702.AT5G37500.1.

Protein family/group databases

TCDBi1.A.1.4.4. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiQ94A76.

Proteomic databases

PaxDbiQ94A76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G37500.1; AT5G37500.1; AT5G37500.
GeneIDi833728.
GrameneiAT5G37500.1; AT5G37500.1; AT5G37500.
KEGGiath:AT5G37500.

Organism-specific databases

TAIRiAT5G37500.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000240830.
InParanoidiQ94A76.
OMAiRITDIDM.
OrthoDBiEOG093602RR.
PhylomeDBiQ94A76.

Enzyme and pathway databases

ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiQ94A76.

Gene expression databases

GenevisibleiQ94A76. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGORK_ARATH
AccessioniPrimary (citable) accession number: Q94A76
Secondary accession number(s): Q9FNY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 30, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.