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Protein

Potassium channel GORK

Gene

GORK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major selective outward-rectifying potassium channel of the guard cell membrane. Involved in regulation of stomatal movements according to the water status. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. Conductance of the channel is modulated in a potassium-dependent fashion. May interact with the cytoskeleton or with regulatory proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi386 – 508123cNMPAdd
BLAST

GO - Molecular functioni

  • ion transmembrane transporter activity Source: TAIR
  • outward rectifier potassium channel activity Source: TAIR

GO - Biological processi

  • ion transport Source: TAIR
  • regulation of membrane potential Source: GO_Central
  • response to abscisic acid Source: TAIR
  • response to cold Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciARA:GQT-1808-MONOMER.
ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Protein family/group databases

TCDBi1.A.1.4.4. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel GORK
Alternative name(s):
Guard cell outward rectifying K(+) channel
Short name:
AtGORK
Gene namesi
Name:GORK
Ordered Locus Names:At5g37500
ORF Names:MPA22.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G37500.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969CytoplasmicSequence analysisAdd
BLAST
Transmembranei70 – 9021Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini91 – 977ExtracellularSequence analysis
Transmembranei98 – 11821Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini119 – 14123CytoplasmicSequence analysisAdd
BLAST
Transmembranei142 – 16221Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini163 – 1686ExtracellularSequence analysis
Transmembranei169 – 18921Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini190 – 20314CytoplasmicSequence analysisAdd
BLAST
Transmembranei204 – 22421Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini225 – 25935ExtracellularSequence analysisAdd
BLAST
Intramembranei260 – 27920Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Topological domaini280 – 2856ExtracellularSequence analysis
Transmembranei286 – 30621Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini307 – 820514CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 820820Potassium channel GORKPRO_0000054129Add
BLAST

Proteomic databases

PaxDbiQ94A76.
PRIDEiQ94A76.

PTM databases

iPTMnetiQ94A76.

Expressioni

Tissue specificityi

Expressed in guard cell-containing tissues, in root epidermal cells and in root hairs. Detected in vascular cells of the root and shoot.2 Publications

Inductioni

Up-regulated under drought, salt stress and cold conditions. Induced by abscisic acid (ABA) treatment in roots and shoots but not in guard cells.1 Publication

Gene expression databases

GenevisibleiQ94A76. AT.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.By similarity

Protein-protein interaction databases

BioGridi18979. 3 interactions.
IntActiQ94A76. 2 interactions.
STRINGi3702.AT5G37500.1.

Structurei

3D structure databases

ProteinModelPortaliQ94A76.
SMRiQ94A76. Positions 320-737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati528 – 55932ANK 1Add
BLAST
Repeati563 – 59230ANK 2Add
BLAST
Repeati596 – 62530ANK 3Add
BLAST
Repeati627 – 65630ANK 4Add
BLAST
Repeati660 – 68930ANK 5Add
BLAST
Repeati693 – 72230ANK 6Add
BLAST
Domaini740 – 82081KHAPROSITE-ProRule annotationAdd
BLAST

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 KHA domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000240830.
InParanoidiQ94A76.
OMAiRITDIDM.
PhylomeDBiQ94A76.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94A76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRLRRRQEI IDHEEEESND DVSSRRGKLS LAETFRWLDS SEHRRIETDG
60 70 80 90 100
HNDYKYIIHP KNRWYKAWEM FILVWAIYSS LFTPMEFGFF RGLPERLFVL
110 120 130 140 150
DIVGQIAFLV DIVLQFFVAY RDTQTYRTVY KPTRIAFRYL KSHFLMDFIG
160 170 180 190 200
CFPWDLIYKA SGKHELVRYL LWIRLFRVRK VVEFFQRLEK DTRINYLFTR
210 220 230 240 250
ILKLLFVEVY CTHTAACIFY YLATTLPPEN EGYTWIGSLK LGDYSYENFR
260 270 280 290 300
EIDLWKRYTT ALYFAIVTMA TVGYGDIHAV NLREMIFVMI YVSFDMVLGA
310 320 330 340 350
YLIGNITALI VKGSNTERFR DKMNDLISFM NRKKLGRDLR SQITGHVRLQ
360 370 380 390 400
YDSHYTDTVM LQDIPASIRA KIAQLLYLPY IKKVPLFKGC STEFINQIVI
410 420 430 440 450
RLHEEYFLPG EVITEQGNVV DHLYFVCEGL LEALVTKTDG SEESVTLLGP
460 470 480 490 500
HTSFGDISII CNISQPFTVR VCELCHLLRL DKQSFSNILE IYFHDGRTIL
510 520 530 540 550
NNIMEEKESN DRIKKLESDI VIHIGKQEAE LALKVNSAAF QGDFYQLKSL
560 570 580 590 600
IRSGADPNKT DYDGRSPLHL AACRGYEDIT LFLIQEGVDV NLKDKFGHTP
610 620 630 640 650
LFEAVKAGQE GVIGLLVKEG ASFNLEDSGN FLCTTVAKGD SDFLKRLLSS
660 670 680 690 700
GMNPNSEDYD HRTPLHVAAS EGLFLMAKML VEAGASVISK DRWGNSPLDE
710 720 730 740 750
ARLCGNKKLI KLLEDVKNAQ SSIYPSSLRE LQEERIERRK CTVFPFHPQE
760 770 780 790 800
AKEERSRKHG VVVWIPSNLE KLIVTAAKEL GLSDGASFVL LSEDQGRITD
810 820
IDMISDGHKL YMISDTTDQT
Length:820
Mass (Da):94,461
Last modified:March 1, 2004 - v2
Checksum:i65F57190056A9AE4
GO

Sequence cautioni

The sequence AAK83636.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251T → S in CAC17380 (PubMed:11113445).Curated
Sequence conflicti230 – 2301N → Q in CAC17380 (PubMed:11113445).Curated
Sequence conflicti306 – 3061I → V in CAC17380 (PubMed:11113445).Curated
Sequence conflicti765 – 7651I → V in CAC17380 (PubMed:11113445).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279009 mRNA. Translation: CAC17380.1.
AB025630 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED94198.1.
AY049294 mRNA. Translation: AAK83636.1. Different initiation.
AY143848 mRNA. Translation: AAN28787.1.
RefSeqiNP_198566.2. NM_123109.4.
UniGeneiAt.10193.

Genome annotation databases

EnsemblPlantsiAT5G37500.1; AT5G37500.1; AT5G37500.
GeneIDi833728.
GrameneiAT5G37500.1; AT5G37500.1; AT5G37500.
KEGGiath:AT5G37500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279009 mRNA. Translation: CAC17380.1.
AB025630 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED94198.1.
AY049294 mRNA. Translation: AAK83636.1. Different initiation.
AY143848 mRNA. Translation: AAN28787.1.
RefSeqiNP_198566.2. NM_123109.4.
UniGeneiAt.10193.

3D structure databases

ProteinModelPortaliQ94A76.
SMRiQ94A76. Positions 320-737.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18979. 3 interactions.
IntActiQ94A76. 2 interactions.
STRINGi3702.AT5G37500.1.

Protein family/group databases

TCDBi1.A.1.4.4. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiQ94A76.

Proteomic databases

PaxDbiQ94A76.
PRIDEiQ94A76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G37500.1; AT5G37500.1; AT5G37500.
GeneIDi833728.
GrameneiAT5G37500.1; AT5G37500.1; AT5G37500.
KEGGiath:AT5G37500.

Organism-specific databases

TAIRiAT5G37500.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000240830.
InParanoidiQ94A76.
OMAiRITDIDM.
PhylomeDBiQ94A76.

Enzyme and pathway databases

BioCyciARA:GQT-1808-MONOMER.
ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiQ94A76.

Gene expression databases

GenevisibleiQ94A76. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K(+)-selective, K(+)-sensing ion channel."
    Ache P., Becker D., Ivashikina N., Dietrich P., Roelfsema M.R.G., Hedrich R.
    FEBS Lett. 486:93-98(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, CHARACTERIZATION.
    Strain: cv. Wassilewskija.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 489-820.
    Strain: cv. Columbia.
  5. "K(+) channel profile and electrical properties of Arabidopsis root hairs."
    Ivashikina N., Becker D., Ache P., Meyerhoff O., Felle H.H., Hedrich R.
    FEBS Lett. 508:463-469(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: GENE FAMILY, NOMENCLATURE.
  7. "The Arabidopsis outward K(+) channel GORK is involved in regulation of stomatal movements and plant transpiration."
    Hosy E., Vavasseur A., Mouline K., Dreyer I., Gaymard F., Poree F., Boucherez J., Lebaudy A., Bouchez D., Very A.-A., Simonneau T., Thibaud J.-B., Sentenac H.
    Proc. Natl. Acad. Sci. U.S.A. 100:5549-5554(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, FUNCTION.
  8. "Regulation of the ABA-sensitive Arabidopsis potassium channel gene GORK in response to water stress."
    Becker D., Hoth S., Ache P., Wenkel S., Roelfsema M.R.G., Meyerhoff O., Hartung W., Hedrich R.
    FEBS Lett. 554:119-126(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiGORK_ARATH
AccessioniPrimary (citable) accession number: Q94A76
Secondary accession number(s): Q9FNY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: February 17, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.