Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-amylase 3, chloroplastic

Gene

AMY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Possesses endoamylolytic activity in vitro, but seems not required for breakdown of transitory starch in leaves. May be involved in the determination of the final structure of glucans by shortening long linear phospho-oligosaccharides in the chloroplast stroma.4 Publications

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei666NucleophileBy similarity1
Active sitei691Proton donorBy similarity1
Binding sitei693SubstrateBy similarity1
Binding sitei695SubstrateBy similarity1
Binding sitei712SubstrateBy similarity1
Binding sitei754SubstrateBy similarity1
Binding sitei773SubstrateBy similarity1
Sitei774Transition state stabilizerBy similarity1
Binding sitei779SubstrateBy similarity1
Binding sitei857SubstrateBy similarity1
Binding sitei884SubstrateBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: CACAO
  • calcium ion binding Source: InterPro

GO - Biological processi

  • starch catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G69830-MONOMER.

Protein family/group databases

CAZyiCBM45. Carbohydrate-Binding Module Family 45.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 3, chloroplastic (EC:3.2.1.1)
Short name:
AtAMY3
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY3
Ordered Locus Names:At1g69830
ORF Names:T17F3.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G69830.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastSequence analysisAdd BLAST55
ChainiPRO_000041886356 – 887Alpha-amylase 3, chloroplasticAdd BLAST832

Proteomic databases

PaxDbiQ94A41.
ProMEXiQ94A41.

PTM databases

iPTMnetiQ94A41.

Expressioni

Tissue specificityi

Expressed in developing siliques.1 Publication

Inductioni

Circadian-regulation. Expression increases during the light phase and decreases during the dark phase.2 Publications

Gene expression databases

GenevisibleiQ94A41. AT.

Interactioni

Protein-protein interaction databases

BioGridi28540. 3 interactors.
STRINGi3702.AT1G69830.1.

Structurei

3D structure databases

ProteinModelPortaliQ94A41.
SMRiQ94A41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni545 – 546Substrate bindingBy similarity2
Regioni664 – 669Substrate bindingBy similarity6
Regioni760 – 762Substrate bindingBy similarity3

Domaini

The N-terminal domain is not required for endoamylolytic activity.1 Publication

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000239525.
InParanoidiQ94A41.
OMAiWEIPAAP.
OrthoDBiEOG093603T9.
PhylomeDBiQ94A41.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94A41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVPIESLL HHSYLRHNSK VNRGNRSFIP ISLNLRSHFT SNKLLHSIGK
60 70 80 90 100
SVGVSSMNKS PVAIRATSSD TAVVETAQSD DVIFKEIFPV QRIEKAEGKI
110 120 130 140 150
YVRLKEVKEK NWELSVGCSI PGKWILHWGV SYVGDTGSEW DQPPEDMRPP
160 170 180 190 200
GSIAIKDYAI ETPLKKLSEG DSFFEVAINL NLESSVAALN FVLKDEETGA
210 220 230 240 250
WYQHKGRDFK VPLVDDVPDN GNLIGAKKGF GALGQLSNIP LKQDKSSAET
260 270 280 290 300
DSIEERKGLQ EFYEEMPISK RVADDNSVSV TARKCPETSK NIVSIETDLP
310 320 330 340 350
GDVTVHWGVC KNGTKKWEIP SEPYPEETSL FKNKALRTRL QRKDDGNGSF
360 370 380 390 400
GLFSLDGKLE GLCFVLKLNE NTWLNYRGED FYVPFLTSSS SPVETEAAQV
410 420 430 440 450
SKPKRKTDKE VSASGFTKEI ITEIRNLAID ISSHKNQKTN VKEVQENILQ
460 470 480 490 500
EIEKLAAEAY SIFRSTTPAF SEEGVLEAEA DKPDIKISSG TGSGFEILCQ
510 520 530 540 550
GFNWESNKSG RWYLELQEKA DELASLGFTV LWLPPPTESV SPEGYMPKDL
560 570 580 590 600
YNLNSRYGTI DELKDTVKKF HKVGIKVLGD AVLNHRCAHF KNQNGVWNLF
610 620 630 640 650
GGRLNWDDRA VVADDPHFQG RGNKSSGDNF HAAPNIDHSQ DFVRKDIKEW
660 670 680 690 700
LCWMMEEVGY DGWRLDFVRG FWGGYVKDYM DASKPYFAVG EYWDSLSYTY
710 720 730 740 750
GEMDYNQDAH RQRIVDWINA TSGAAGAFDV TTKGILHTAL QKCEYWRLSD
760 770 780 790 800
PKGKPPGVVG WWPSRAVTFI ENHDTGSTQG HWRFPEGKEM QGYAYILTHP
810 820 830 840 850
GTPAVFFDHI FSDYHSEIAA LLSLRNRQKL HCRSEVNIDK SERDVYAAII
860 870 880
DEKVAMKIGP GHYEPPNGSQ NWSVAVEGRD YKVWETS
Length:887
Mass (Da):99,842
Last modified:December 1, 2001 - v1
Checksum:i8EA417384B1AEB69
GO

Sequence cautioni

The sequence AAG52558 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010675 Genomic DNA. Translation: AAG52558.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34983.1.
AY050398 mRNA. Translation: AAK91414.1.
BT000643 mRNA. Translation: AAN18209.1.
PIRiE96720.
RefSeqiNP_564977.1. NM_105651.5.
UniGeneiAt.24555.
At.72024.

Genome annotation databases

EnsemblPlantsiAT1G69830.1; AT1G69830.1; AT1G69830.
GeneIDi843319.
GrameneiAT1G69830.1; AT1G69830.1; AT1G69830.
KEGGiath:AT1G69830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010675 Genomic DNA. Translation: AAG52558.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34983.1.
AY050398 mRNA. Translation: AAK91414.1.
BT000643 mRNA. Translation: AAN18209.1.
PIRiE96720.
RefSeqiNP_564977.1. NM_105651.5.
UniGeneiAt.24555.
At.72024.

3D structure databases

ProteinModelPortaliQ94A41.
SMRiQ94A41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28540. 3 interactors.
STRINGi3702.AT1G69830.1.

Protein family/group databases

CAZyiCBM45. Carbohydrate-Binding Module Family 45.
GH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiQ94A41.

Proteomic databases

PaxDbiQ94A41.
ProMEXiQ94A41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G69830.1; AT1G69830.1; AT1G69830.
GeneIDi843319.
GrameneiAT1G69830.1; AT1G69830.1; AT1G69830.
KEGGiath:AT1G69830.

Organism-specific databases

TAIRiAT1G69830.

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000239525.
InParanoidiQ94A41.
OMAiWEIPAAP.
OrthoDBiEOG093603T9.
PhylomeDBiQ94A41.

Enzyme and pathway databases

BioCyciARA:AT1G69830-MONOMER.

Miscellaneous databases

PROiQ94A41.

Gene expression databases

GenevisibleiQ94A41. AT.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY3_ARATH
AccessioniPrimary (citable) accession number: Q94A41
Secondary accession number(s): Q9CAR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.