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Protein

Aconitate hydratase 3, mitochondrial

Gene

ACO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.By similarity

Catalytic activityi

Citrate = isocitrate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase 5, mitochondrial (CSY5), Citrate synthase 1, peroxisomal (CSY1), Citrate synthase 2, peroxisomal (CSY2), Citrate synthase 3, peroxisomal (CSY3), Citrate synthase 4, mitochondrial (CSY4)
  2. Aconitate hydratase 2, mitochondrial (ACO2), Aconitate hydratase 1 (ACO1), Aconitate hydratase 3, mitochondrial (ACO3)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei187 – 1871SubstrateBy similarity
Metal bindingi538 – 5381Iron-sulfur (4Fe-4S)By similarity
Metal bindingi604 – 6041Iron-sulfur (4Fe-4S)By similarity
Metal bindingi607 – 6071Iron-sulfur (4Fe-4S)By similarity
Binding sitei637 – 6371SubstrateBy similarity
Binding sitei642 – 6421SubstrateBy similarity
Binding sitei800 – 8001SubstrateBy similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • aconitate hydratase activity Source: TAIR
  • copper ion binding Source: TAIR

GO - Biological processi

  • citrate metabolic process Source: TAIR
  • glyoxylate cycle Source: UniProtKB-KW
  • isocitrate metabolic process Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to oxidative stress Source: TAIR
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase 3, mitochondrial (EC:4.2.1.3)
Short name:
Aconitase 3
Alternative name(s):
Citrate hydro-lyase 3
Gene namesi
Name:ACO3
Ordered Locus Names:At4g26970
ORF Names:F10M23.310
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26970.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytosol Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8383MitochondrionSequence analysisAdd
BLAST
Chaini84 – 995912Aconitate hydratase 3, mitochondrialPRO_0000259922Add
BLAST

Proteomic databases

PaxDbiQ94A28.
PRIDEiQ94A28.

PTM databases

iPTMnetiQ94A28.

Expressioni

Gene expression databases

GenevisibleiQ94A28. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi14092. 1 interaction.
STRINGi3702.AT4G26970.1.

Structurei

3D structure databases

ProteinModelPortaliQ94A28.
SMRiQ94A28. Positions 121-993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni306 – 3083Substrate bindingBy similarity
Regioni881 – 8822Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0452. Eukaryota.
COG1048. LUCA.
HOGENOMiHOG000025703.
InParanoidiQ94A28.
KOiK01681.
OMAiISPAGAW.
OrthoDBiEOG093601QZ.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR006249. Aconitase/IRP2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01341. aconitase_1. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94A28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRRATSGVR SASARLSSSL SRIASSETAS VSAPSASSLR NQTNRSKSFS
60 70 80 90 100
SALRSFRVCS ASTRWSHGGS WGSPASLRAQ ARNSTPVMEK FERKYATMAS
110 120 130 140 150
EHSYKDILTS LPKPGGGEYG KYYSLPALND PRIDKLPFSV RILLESAIRN
160 170 180 190 200
CDNYQVTKDD VEKILDWENT STKQVEIAFK PARVILQDFT GVPVLVDLAS
210 220 230 240 250
MRDAVKNLGS DPSKINPLVP VDLVVDHSIQ VDFARSEDAA QKNLELEFKR
260 270 280 290 300
NKERFTFLKW GSTAFQNMLV VPPGSGIVHQ VNLEYLGRVV FNSKGFLYPD
310 320 330 340 350
SVVGTDSHTT MIDGLGVAGW GVGGIEAEAA MLGQPMSMVL PGVVGFKLDG
360 370 380 390 400
KLKEGVTATD LVLTVTQILR KHGVVGKFVE FYGEGMSELS LADRATIANM
410 420 430 440 450
SPEYGATMGF FPVDHVTLEY LKLTGRSDET VSMIESYLRA NNMFVDYNEP
460 470 480 490 500
QQERAYTSYL QLDLGHVEPC ISGPKRPHDR VPLKDMKADW HACLDNPVGF
510 520 530 540 550
KGFAVPKEKQ EEVVKFSYNG QPAEIKHGSV VIAAITSCTN TSNPSVMIGA
560 570 580 590 600
ALVAKKASDL GLKVKPWVKT SLAPGSRVVE KYLDRSGLRE SLTKQGFEIV
610 620 630 640 650
GYGCTTCIGN SGNLDPEVAS AIEGTDIIPA AVLSGNRNFE GRVHPQTRAN
660 670 680 690 700
YLASPPLVVA YALAGTVDID FEKEPIGTRS DGKSVYLRDV WPSNEEVAQV
710 720 730 740 750
VQYSVLPSMF KSSYETITEG NPLWNELSAP SSTLYSWDPN STYIHEPPYF
760 770 780 790 800
KNMTANPPGP REVKDAYCLL NFGDSVTTDH ISPAGNIQKT SPAAKFLMDR
810 820 830 840 850
GVISEDFNSY GSRRGNDEVM ARGTFANIRI VNKLLKGEVG PNTVHIPTGE
860 870 880 890 900
KLSVFDAASK YKTAEQDTII LAGAEYGSGS SRDWAAKGPL LLGVKAVIAK
910 920 930 940 950
SFERIHRSNL AGMGIIPLCF KAGEDAETLG LTGHERYTVH LPTKVSDIRP
960 970 980 990
GQDVTVTTDS GKSFVCTLRF DTEVELAYYD HGGILPYVIR SLSAK
Length:995
Mass (Da):108,481
Last modified:May 3, 2011 - v3
Checksum:i20FC18030F1CBDDB
GO

Sequence cautioni

The sequence CAB36543 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79552 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti407 – 4071T → A in AAK91447 (PubMed:14593172).Curated
Sequence conflicti407 – 4071T → A in AAN18061 (PubMed:14593172).Curated
Sequence conflicti463 – 4631D → E in CAB36543 (PubMed:10617198).Curated
Sequence conflicti463 – 4631D → E in CAB79552 (PubMed:10617198).Curated
Sequence conflicti903 – 9031E → G in AAK91447 (PubMed:14593172).Curated
Sequence conflicti903 – 9031E → G in AAN18061 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035440 Genomic DNA. Translation: CAB36543.1. Sequence problems.
AL161566 Genomic DNA. Translation: CAB79552.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85279.1.
AY050431 mRNA. Translation: AAK91447.1.
BT000492 mRNA. Translation: AAN18061.1.
PIRiT04820.
RefSeqiNP_567763.2. NM_118831.4.
UniGeneiAt.22696.
At.48905.

Genome annotation databases

EnsemblPlantsiAT4G26970.1; AT4G26970.1; AT4G26970.
GeneIDi828805.
GrameneiAT4G26970.1; AT4G26970.1; AT4G26970.
KEGGiath:AT4G26970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035440 Genomic DNA. Translation: CAB36543.1. Sequence problems.
AL161566 Genomic DNA. Translation: CAB79552.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85279.1.
AY050431 mRNA. Translation: AAK91447.1.
BT000492 mRNA. Translation: AAN18061.1.
PIRiT04820.
RefSeqiNP_567763.2. NM_118831.4.
UniGeneiAt.22696.
At.48905.

3D structure databases

ProteinModelPortaliQ94A28.
SMRiQ94A28. Positions 121-993.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14092. 1 interaction.
STRINGi3702.AT4G26970.1.

PTM databases

iPTMnetiQ94A28.

Proteomic databases

PaxDbiQ94A28.
PRIDEiQ94A28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26970.1; AT4G26970.1; AT4G26970.
GeneIDi828805.
GrameneiAT4G26970.1; AT4G26970.1; AT4G26970.
KEGGiath:AT4G26970.

Organism-specific databases

TAIRiAT4G26970.

Phylogenomic databases

eggNOGiKOG0452. Eukaryota.
COG1048. LUCA.
HOGENOMiHOG000025703.
InParanoidiQ94A28.
KOiK01681.
OMAiISPAGAW.
OrthoDBiEOG093601QZ.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.

Miscellaneous databases

PROiQ94A28.

Gene expression databases

GenevisibleiQ94A28. AT.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR006249. Aconitase/IRP2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01341. aconitase_1. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACO3M_ARATH
AccessioniPrimary (citable) accession number: Q94A28
Secondary accession number(s): Q9SZ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 3, 2011
Last modified: September 7, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.