Reviewed, UniProtKB/Swiss-Prot Q94A16 (CP21C_ARATH)

Names and origin

Protein names

Recommended name:
    Peptidyl-prolyl cis-trans isomerase CYP21-3, mitochondrial
      Short name=PPIase CYP21-3
      Short name=Rotamase CYP21-3
    EC=5.2.1.8
Alternative name(s):
    Cyclophilin of 21 kDa 3

Gene names

Name:	CYP21-3
Ordered Locus Names:	At2g47320
ORF Names:	T8I13.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase By similarity.
Subcellular location	Mitochondrion Potential.
Tissue specificity	Ubiquitous, mostly in aerial organs. Ref.1 Ref.4
Induction	Repressed by the munition hexahydro-1,3,5-trinitro-1,3,5-triazine, also known as Royal Demolition eXplosive (RDX). Ref.5
Sequence similarities	Belongs to the cyclophilin-type PPIase family. Contains 1 PPIase cyclophilin-type domain.
Sequence caution	The sequence AAK91465.1 differs from that shown. Reason: Erroneous termination at position 82. Translated as Leu.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Cyclosporin
Molecular function	Chaperone Isomerase Rotamase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptide binding Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 230		Peptidyl-prolyl cis-trans isomerase CYP21-3, mitochondrial		PRO_0000044628
Regions
	Domain	76 – 226	151	PPIase cyclophilin-type

Sequences

	Sequence		Length	Mass (Da)
	Q94A16-1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 6198D43EE5B9552F	FASTA	230	26,042
	10 20 30 40 50 60 MAKIKPQALL QQSKKKKGPS RISITNIVIY TLAVLLLVFV LFSAYRRWTH RSEIPTHNGR 70 80 90 100 110 120 SVLEDAAFPG MKNVDLPRFA TLDTGKGSVT IELFKDTAPN VVDQFMKFCQ DGYFKGFLFS 130 140 150 160 170 180 RVVKHFVIQA GDSAEFDAVK DWALDRKNID TSLKHEEFMV GTPKAKNEQG GFEFFIVSAQ 190 200 210 220 230 IKDLNEKLTV FGRVSKGQDV VQEIEEVETD DQYQPKSPIE IMSVTLLQDM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis cyclophilin gene family." Romano P.G.N., Horton P., Gray J.E. Plant Physiol. 134:1268-1282(2004) [PubMed: 15051864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Immunophilins and parvulins. Superfamily of peptidyl prolyl isomerases in Arabidopsis." He Z., Li L., Luan S. Plant Physiol. 134:1248-1267(2004) [PubMed: 15047905] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Gene expression changes in Arabidopsis thaliana seedling roots exposed to the munition hexahydro-1,3,5-trinitro-1,3,5-triazine." Ekman D.R., Wolfe N.L., Dean J.F.D. Environ. Sci. Technol. 39:6313-6320(2005) [PubMed: 16173598] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY568520 mRNA. Translation: AAS75303.1. AC002337 Genomic DNA. Translation: AAB63832.1. AY050450 mRNA. Translation: AAK91465.1. Different initiation. AY097351 mRNA. Translation: AAM19867.1.
IPI	IPI00535705.
PIR	G84913.
RefSeq	NP_182254.1.
UniGene	At.12226 At.25582 Rsa.1238
3D structure databases
SMR	Q94A16. Positions 79-229.
ModBase	Search...
Protein-protein interaction databases
STRING	Q94A16.
Proteomic databases
PRIDE	Q94A16.
Genome annotation databases
GeneID	819345.
GenomeReviews	Gene locus AT2G47320 in contig CT485783_GR.
KEGG	ath:AT2G47320.
NMPDR	fig|3702.1.peg.11919.
Organism-specific databases
TAIR	At2g47320.
Phylogenomic databases
eggNOG	KOG0882.
HOGENOM	HBG599725.
Enzyme and pathway databases
BRENDA	5.2.1.8. 302.
Gene expression databases
Genevestigator	Q94A16.
GermOnline	AT2G47320. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR015891. Cyclophilin-like. IPR002130. PPIase_cyclophilin. [Graphical view]
Gene3D	G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit.
Pfam	PF00160. Pro_isomerase. 1 hit. [Graphical view]
PRINTS	PR00153. CSAPPISMRASE.
PROSITE	PS00170. CSA_PPIASE_1. False negative. PS50072. CSA_PPIASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP21C_ARATH

Accession

Primary (citable) accession number: Q94A16
Secondary accession number(s): O22906

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	February 9, 2010
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)