ID   RFS2_ARATH              Reviewed;         773 AA.
AC   Q94A08; Q0WWI0; Q3EAI2; Q3EAI3; Q9SCM1;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 2.
DT   27-MAR-2024, entry version 111.
DE   RecName: Full=Probable galactinol--sucrose galactosyltransferase 2;
DE            EC=2.4.1.82;
DE   AltName: Full=Protein SEED IMBIBITION 2;
DE   AltName: Full=Raffinose synthase 2;
GN   Name=RFS2; Synonyms=RS2, SIP2; OrderedLocusNames=At3g57520;
GN   ORFNames=T8H10.120;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18502973; DOI=10.1104/pp.108.122465;
RA   Nishizawa A., Yabuta Y., Shigeoka S.;
RT   "Galactinol and raffinose constitute a novel function to protect plants
RT   from oxidative damage.";
RL   Plant Physiol. 147:1251-1263(2008).
CC   -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC       Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC       seeds, roots and tubers (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC         inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC         ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q94A08-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q94A08-2; Sequence=VSP_038414, VSP_038415;
CC       Name=3;
CC         IsoId=Q94A08-3; Sequence=VSP_038412, VSP_038413;
CC   -!- INDUCTION: By oxidative stress. {ECO:0000269|PubMed:18502973}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL133248; CAB66109.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE79665.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE79667.1; -; Genomic_DNA.
DR   EMBL; AY050772; AAK92707.1; -; mRNA.
DR   EMBL; AK226370; BAE98518.1; -; mRNA.
DR   PIR; T46188; T46188.
DR   RefSeq; NP_191311.1; NM_115612.3. [Q94A08-1]
DR   RefSeq; NP_850715.1; NM_180384.1. [Q94A08-2]
DR   AlphaFoldDB; Q94A08; -.
DR   SMR; Q94A08; -.
DR   BioGRID; 10235; 2.
DR   STRING; 3702.Q94A08; -.
DR   CAZy; GH36; Glycoside Hydrolase Family 36.
DR   PaxDb; 3702-AT3G57520-1; -.
DR   ProteomicsDB; 236235; -. [Q94A08-1]
DR   EnsemblPlants; AT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
DR   EnsemblPlants; AT3G57520.2; AT3G57520.2; AT3G57520. [Q94A08-2]
DR   GeneID; 824919; -.
DR   Gramene; AT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
DR   Gramene; AT3G57520.2; AT3G57520.2; AT3G57520. [Q94A08-2]
DR   KEGG; ath:AT3G57520; -.
DR   Araport; AT3G57520; -.
DR   TAIR; AT3G57520; SIP2.
DR   eggNOG; ENOG502QPVE; Eukaryota.
DR   HOGENOM; CLU_007066_0_0_1; -.
DR   InParanoid; Q94A08; -.
DR   OMA; HVCPVMT; -.
DR   OrthoDB; 214587at2759; -.
DR   PhylomeDB; Q94A08; -.
DR   BioCyc; ARA:AT3G57520-MONOMER; -.
DR   BioCyc; MetaCyc:AT3G57520-MONOMER; -.
DR   BRENDA; 2.4.1.82; 399.
DR   PRO; PR:Q94A08; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q94A08; baseline and differential.
DR   GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0052692; F:raffinose alpha-galactosidase activity; IDA:TAIR.
DR   GO; GO:0034484; P:raffinose catabolic process; IDA:TAIR.
DR   GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR008811; Glycosyl_hydrolases_36.
DR   PANTHER; PTHR31268; -; 1.
DR   PANTHER; PTHR31268:SF32; GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 2-RELATED; 1.
DR   Pfam; PF05691; Raffinose_syn; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   Genevisible; Q94A08; AT.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Carbohydrate metabolism; Glycosyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..773
FT                   /note="Probable galactinol--sucrose galactosyltransferase
FT                   2"
FT                   /id="PRO_0000389256"
FT   VAR_SEQ         552..565
FT                   /note="SLLKIWNMNKFTGI -> RCWLVQGNEEEPD (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_038412"
FT   VAR_SEQ         566..773
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_038413"
FT   VAR_SEQ         655..656
FT                   /note="EI -> DL (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_038414"
FT   VAR_SEQ         657..773
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_038415"
FT   CONFLICT        255
FT                   /note="Q -> R (in Ref. 3; AAK92707)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   773 AA;  85143 MW;  0852F9E67952C8D3 CRC64;
     MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE QSKSLHVFPI
     GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE SKDEVEGNGD DAPTVYTVFL
     PLLEGQFRAV LQGNEKNEIE ICFESGDKAV ETSQGTHLVY VHAGTNPFEV IRQSVKAVER
     HMQTFHHREK KKLPSFLDWF GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW
     QQIENKEKDE NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK
     QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL AVHGLGLVNP
     KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR VSLTRSYQQA LEASIARNFT
     DNGCISCMCH NTDGLYSAKQ TAIVRASDDF YPRDPASHTI HIASVAYNSL FLGEFMQPDW
     DMFHSLHPTA EYHAAARAVG GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD
     CLFADPARDG ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD
     DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI SPLKEITENI
     SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA SPALSDNRSP TALVSVSVRG
     CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV GLVTLNLPVT REEMFRWHVE ILV
//