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Q94A08

- RFS2_ARATH

UniProt

Q94A08 - RFS2_ARATH

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Protein

Probable galactinol--sucrose galactosyltransferase 2

Gene
RFS2, RS2, SIP2, At3g57520, T8H10.120
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

  1. raffinose alpha-galactosidase activity Source: TAIR
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. raffinose catabolic process Source: TAIR
  2. response to karrikin Source: TAIR
  3. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT3G57520-MONOMER.
ARA:GQT-2348-MONOMER.
ARA:GQT-2349-MONOMER.
MetaCyc:AT3G57520-MONOMER.

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 2 (EC:2.4.1.82)
Alternative name(s):
Protein SEED IMBIBITION 2
Raffinose synthase 2
Gene namesi
Name:RFS2
Synonyms:RS2, SIP2
Ordered Locus Names:At3g57520
ORF Names:T8H10.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G57520.

Subcellular locationi

GO - Cellular componenti

  1. plasmodesma Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773Probable galactinol--sucrose galactosyltransferase 2PRO_0000389256Add
BLAST

Proteomic databases

PaxDbiQ94A08.
PRIDEiQ94A08.

Expressioni

Inductioni

By oxidative stress.1 Publication

Gene expression databases

ArrayExpressiQ94A08.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G57520.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ94A08.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG06986.
HOGENOMiHOG000237551.
InParanoidiQ9SCM1.
KOiK06617.
OMAiKNEIEIC.
PhylomeDBiQ94A08.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamiPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q94A08-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE    50
QSKSLHVFPI GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE 100
SKDEVEGNGD DAPTVYTVFL PLLEGQFRAV LQGNEKNEIE ICFESGDKAV 150
ETSQGTHLVY VHAGTNPFEV IRQSVKAVER HMQTFHHREK KKLPSFLDWF 200
GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW QQIENKEKDE 250
NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK 300
QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL 350
AVHGLGLVNP KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR 400
VSLTRSYQQA LEASIARNFT DNGCISCMCH NTDGLYSAKQ TAIVRASDDF 450
YPRDPASHTI HIASVAYNSL FLGEFMQPDW DMFHSLHPTA EYHAAARAVG 500
GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD CLFADPARDG 550
ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD 600
DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI 650
SPLKEITENI SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA 700
SPALSDNRSP TALVSVSVRG CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV 750
GLVTLNLPVT REEMFRWHVE ILV 773
Length:773
Mass (Da):85,143
Last modified:November 24, 2009 - v2
Checksum:i0852F9E67952C8D3
GO
Isoform 2 (identifier: Q94A08-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     655-656: EI → DL
     657-773: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:656
Mass (Da):72,374
Checksum:i7128B242BF0A1AB1
GO
Isoform 3 (identifier: Q94A08-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     552-565: SLLKIWNMNKFTGI → RCWLVQGNEEEPD
     566-773: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:564
Mass (Da):62,424
Checksum:i87D74AE3896A5D1A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei552 – 56514SLLKI…KFTGI → RCWLVQGNEEEPD in isoform 3. VSP_038412Add
BLAST
Alternative sequencei566 – 773208Missing in isoform 3. VSP_038413Add
BLAST
Alternative sequencei655 – 6562EI → DL in isoform 2. VSP_038414
Alternative sequencei657 – 773117Missing in isoform 2. VSP_038415Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551Q → R in AAK92707. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL133248 Genomic DNA. Translation: CAB66109.1.
CP002686 Genomic DNA. Translation: AEE79665.1.
CP002686 Genomic DNA. Translation: AEE79667.1.
AY050772 mRNA. Translation: AAK92707.1.
AK226370 mRNA. Translation: BAE98518.1.
PIRiT46188.
RefSeqiNP_191311.1. NM_115612.2. [Q94A08-1]
NP_850715.1. NM_180384.1. [Q94A08-2]
UniGeneiAt.22207.
At.30900.

Genome annotation databases

EnsemblPlantsiAT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
GeneIDi824919.
KEGGiath:AT3G57520.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL133248 Genomic DNA. Translation: CAB66109.1 .
CP002686 Genomic DNA. Translation: AEE79665.1 .
CP002686 Genomic DNA. Translation: AEE79667.1 .
AY050772 mRNA. Translation: AAK92707.1 .
AK226370 mRNA. Translation: BAE98518.1 .
PIRi T46188.
RefSeqi NP_191311.1. NM_115612.2. [Q94A08-1 ]
NP_850715.1. NM_180384.1. [Q94A08-2 ]
UniGenei At.22207.
At.30900.

3D structure databases

ProteinModelPortali Q94A08.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G57520.1-P.

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbi Q94A08.
PRIDEi Q94A08.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G57520.1 ; AT3G57520.1 ; AT3G57520 . [Q94A08-1 ]
GeneIDi 824919.
KEGGi ath:AT3G57520.

Organism-specific databases

TAIRi AT3G57520.

Phylogenomic databases

eggNOGi NOG06986.
HOGENOMi HOG000237551.
InParanoidi Q9SCM1.
KOi K06617.
OMAi KNEIEIC.
PhylomeDBi Q94A08.

Enzyme and pathway databases

BioCyci ARA:AT3G57520-MONOMER.
ARA:GQT-2348-MONOMER.
ARA:GQT-2349-MONOMER.
MetaCyc:AT3G57520-MONOMER.

Gene expression databases

ArrayExpressi Q94A08.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view ]
Pfami PF05691. Raffinose_syn. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
    Nishizawa A., Yabuta Y., Shigeoka S.
    Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiRFS2_ARATH
AccessioniPrimary (citable) accession number: Q94A08
Secondary accession number(s): Q0WWI0
, Q3EAI2, Q3EAI3, Q9SCM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: May 14, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi