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Q94A08 (RFS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable galactinol--sucrose galactosyltransferase 2

EC=2.4.1.82
Alternative name(s):
Protein SEED IMBIBITION 2
Raffinose synthase 2
Gene names
Name:RFS2
Synonyms:RS2, SIP2
Ordered Locus Names:At3g57520
ORF Names:T8H10.120
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length773 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

Induction

By oxidative stress. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q94A08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q94A08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     655-656: EI → DL
     657-773: Missing.
Note: Derived from EST data. No experimental confirmation available.
Isoform 3 (identifier: Q94A08-3)

The sequence of this isoform differs from the canonical sequence as follows:
     552-565: SLLKIWNMNKFTGI → RCWLVQGNEEEPD
     566-773: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 773773Probable galactinol--sucrose galactosyltransferase 2
PRO_0000389256

Natural variations

Alternative sequence552 – 56514SLLKI…KFTGI → RCWLVQGNEEEPD in isoform 3.
VSP_038412
Alternative sequence566 – 773208Missing in isoform 3.
VSP_038413
Alternative sequence655 – 6562EI → DL in isoform 2.
VSP_038414
Alternative sequence657 – 773117Missing in isoform 2.
VSP_038415

Experimental info

Sequence conflict2551Q → R in AAK92707. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: 0852F9E67952C8D3

FASTA77385,143
        10         20         30         40         50         60 
MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE QSKSLHVFPI 

        70         80         90        100        110        120 
GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE SKDEVEGNGD DAPTVYTVFL 

       130        140        150        160        170        180 
PLLEGQFRAV LQGNEKNEIE ICFESGDKAV ETSQGTHLVY VHAGTNPFEV IRQSVKAVER 

       190        200        210        220        230        240 
HMQTFHHREK KKLPSFLDWF GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW 

       250        260        270        280        290        300 
QQIENKEKDE NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK 

       310        320        330        340        350        360 
QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL AVHGLGLVNP 

       370        380        390        400        410        420 
KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR VSLTRSYQQA LEASIARNFT 

       430        440        450        460        470        480 
DNGCISCMCH NTDGLYSAKQ TAIVRASDDF YPRDPASHTI HIASVAYNSL FLGEFMQPDW 

       490        500        510        520        530        540 
DMFHSLHPTA EYHAAARAVG GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD 

       550        560        570        580        590        600 
CLFADPARDG ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD 

       610        620        630        640        650        660 
DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI SPLKEITENI 

       670        680        690        700        710        720 
SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA SPALSDNRSP TALVSVSVRG 

       730        740        750        760        770 
CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV GLVTLNLPVT REEMFRWHVE ILV 

« Hide

Isoform 2 [UniParc].

Checksum: 7128B242BF0A1AB1
Show »

FASTA65672,374
Isoform 3 [UniParc].

Checksum: 87D74AE3896A5D1A
Show »

FASTA56462,424

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1).
Strain: cv. Columbia.
[5]"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
Nishizawa A., Yabuta Y., Shigeoka S.
Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL133248 Genomic DNA. Translation: CAB66109.1.
CP002686 Genomic DNA. Translation: AEE79665.1.
CP002686 Genomic DNA. Translation: AEE79667.1.
AY050772 mRNA. Translation: AAK92707.1.
AK226370 mRNA. Translation: BAE98518.1.
PIRT46188.
RefSeqNP_191311.1. NM_115612.2.
NP_850715.1. NM_180384.1.
UniGeneAt.22207.
At.30900.

3D structure databases

ProteinModelPortalQ94A08.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G57520.1-P.

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbQ94A08.
PRIDEQ94A08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
GeneID824919.
KEGGath:AT3G57520.

Organism-specific databases

TAIRAT3G57520.

Phylogenomic databases

eggNOGNOG06986.
HOGENOMHOG000237551.
InParanoidQ9SCM1.
KOK06617.
OMAETQFMLL.
PhylomeDBQ94A08.
ProtClustDBPLN02219.

Enzyme and pathway databases

BioCycARA:AT3G57520-MONOMER.
ARA:GQT-2348-MONOMER.
ARA:GQT-2349-MONOMER.
MetaCyc:AT3G57520-MONOMER.

Gene expression databases

ArrayExpressQ94A08.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
ProtoNetSearch...

Entry information

Entry nameRFS2_ARATH
AccessionPrimary (citable) accession number: Q94A08
Secondary accession number(s): Q0WWI0 expand/collapse secondary AC list , Q3EAI2, Q3EAI3, Q9SCM1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: April 16, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names