Q94A08 (RFS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactinol--sucrose galactosyltransferase 2
EC=2.4.1.82

Alternative name(s):
Protein SEED IMBIBITION 2
Raffinose synthase 2

Gene names

Name:	RFS2
Synonyms:	RS2, SIP2
Ordered Locus Names:	At3g57520
ORF Names:	T8H10.120

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.
Catalytic activity	Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.
Induction	By oxidative stress. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolases 36 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	raffinose catabolic process Inferred from direct assay PubMed 20739305. Source: TAIR response to karrikin Inferred from expression pattern PubMed 20351290. Source: TAIR response to oxidative stress Inferred from expression pattern Ref.5. Source: TAIR
Cellular_component	plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	raffinose alpha-galactosidase activity Inferred from direct assay PubMed 20739305. Source: TAIR transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q94A08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q94A08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     655-656: EI → DL
     657-773: Missing.

Note: Derived from EST data. No experimental confirmation available.

Isoform 3 (identifier: Q94A08-3)

The sequence of this isoform differs from the canonical sequence as follows:
     552-565: SLLKIWNMNKFTGI → RCWLVQGNEEEPD
     566-773: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	Probable galactinol--sucrose galactosyltransferase 2		PRO_0000389256
Natural variations
	Alternative sequence	552 – 565	14	SLLKI…KFTGI → RCWLVQGNEEEPD in isoform 3.		VSP_038412
	Alternative sequence	566 – 773	208	Missing in isoform 3.		VSP_038413
	Alternative sequence	655 – 656	2	EI → DL in isoform 2.		VSP_038414
	Alternative sequence	657 – 773	117	Missing in isoform 2.		VSP_038415
Experimental info
	Sequence conflict	255	1	Q → R in AAK92707. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: 0852F9E67952C8D3	FASTA	773	85,143
	10 20 30 40 50 60 MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE QSKSLHVFPI 70 80 90 100 110 120 GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE SKDEVEGNGD DAPTVYTVFL 130 140 150 160 170 180 PLLEGQFRAV LQGNEKNEIE ICFESGDKAV ETSQGTHLVY VHAGTNPFEV IRQSVKAVER 190 200 210 220 230 240 HMQTFHHREK KKLPSFLDWF GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW 250 260 270 280 290 300 QQIENKEKDE NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK 310 320 330 340 350 360 QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL AVHGLGLVNP 370 380 390 400 410 420 KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR VSLTRSYQQA LEASIARNFT 430 440 450 460 470 480 DNGCISCMCH NTDGLYSAKQ TAIVRASDDF YPRDPASHTI HIASVAYNSL FLGEFMQPDW 490 500 510 520 530 540 DMFHSLHPTA EYHAAARAVG GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD 550 560 570 580 590 600 CLFADPARDG ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD 610 620 630 640 650 660 DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI SPLKEITENI 670 680 690 700 710 720 SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA SPALSDNRSP TALVSVSVRG 730 740 750 760 770 CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV GLVTLNLPVT REEMFRWHVE ILV
	Isoform 2 [UniParc]. Checksum: 7128B242BF0A1AB1	FASTA	656	72,374
	10 20 30 40 50 60 MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE QSKSLHVFPI 70 80 90 100 110 120 GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE SKDEVEGNGD DAPTVYTVFL 130 140 150 160 170 180 PLLEGQFRAV LQGNEKNEIE ICFESGDKAV ETSQGTHLVY VHAGTNPFEV IRQSVKAVER 190 200 210 220 230 240 HMQTFHHREK KKLPSFLDWF GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW 250 260 270 280 290 300 QQIENKEKDE NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK 310 320 330 340 350 360 QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL AVHGLGLVNP 370 380 390 400 410 420 KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR VSLTRSYQQA LEASIARNFT 430 440 450 460 470 480 DNGCISCMCH NTDGLYSAKQ TAIVRASDDF YPRDPASHTI HIASVAYNSL FLGEFMQPDW 490 500 510 520 530 540 DMFHSLHPTA EYHAAARAVG GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD 550 560 570 580 590 600 CLFADPARDG ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD 610 620 630 640 650 DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI SPLKDL
	Isoform 3 [UniParc]. Checksum: 87D74AE3896A5D1A	FASTA	564	62,424
	10 20 30 40 50 60 MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE QSKSLHVFPI 70 80 90 100 110 120 GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE SKDEVEGNGD DAPTVYTVFL 130 140 150 160 170 180 PLLEGQFRAV LQGNEKNEIE ICFESGDKAV ETSQGTHLVY VHAGTNPFEV IRQSVKAVER 190 200 210 220 230 240 HMQTFHHREK KKLPSFLDWF GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW 250 260 270 280 290 300 QQIENKEKDE NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK 310 320 330 340 350 360 QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL AVHGLGLVNP 370 380 390 400 410 420 KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR VSLTRSYQQA LEASIARNFT 430 440 450 460 470 480 DNGCISCMCH NTDGLYSAKQ TAIVRASDDF YPRDPASHTI HIASVAYNSL FLGEFMQPDW 490 500 510 520 530 540 DMFHSLHPTA EYHAAARAVG GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD 550 560 CLFADPARDG IRCWLVQGNE EEPD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1). Strain: cv. Columbia.
[5]	"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage." Nishizawa A., Yabuta Y., Shigeoka S. Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL133248 Genomic DNA. Translation: CAB66109.1. CP002686 Genomic DNA. Translation: AEE79665.1. CP002686 Genomic DNA. Translation: AEE79667.1. AY050772 mRNA. Translation: AAK92707.1. AK226370 mRNA. Translation: BAE98518.1.
IPI	IPI00526258. IPI00541537. IPI00544535.
PIR	T46188.
RefSeq	NP_191311.1. NM_115612.2. NP_850715.1. NM_180384.1.
UniGene	At.22207. At.30900.
3D structure databases
ProteinModelPortal	Q94A08.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G57520.1-P.
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Proteomic databases
PaxDb	Q94A08.
PRIDE	Q94A08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G57520.1; AT3G57520.1; AT3G57520.
GeneID	824919.
KEGG	ath:AT3G57520.
Organism-specific databases
TAIR	At3g57520.
Phylogenomic databases
eggNOG	NOG06986.
HOGENOM	HOG000237551.
InParanoid	Q9SCM1.
KO	K06617.
OMA	FHHREKK.
PhylomeDB	Q94A08.
ProtClustDB	PLN02219.
Enzyme and pathway databases
BioCyc	ARA:AT3G57520-MONOMER. MetaCyc:AT3G57520-MONOMER.
Gene expression databases
ArrayExpress	Q94A08.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR008811. Raffinose_synthase. [Graphical view]
Pfam	PF05691. Raffinose_syn. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFS2_ARATH

Accession

Primary (citable) accession number: Q94A08
Secondary accession number(s): Q0WWI0 , Q3EAI2, Q3EAI3, Q9SCM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program