Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q94A08

- RFS2_ARATH

UniProt

Q94A08 - RFS2_ARATH

Protein

Probable galactinol--sucrose galactosyltransferase 2

Gene

RFS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (24 Nov 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.By similarity

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

    GO - Molecular functioni

    1. raffinose alpha-galactosidase activity Source: TAIR
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. raffinose catabolic process Source: TAIR
    2. response to karrikin Source: TAIR
    3. response to oxidative stress Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciARA:AT3G57520-MONOMER.
    ARA:GQT-2348-MONOMER.
    ARA:GQT-2349-MONOMER.
    MetaCyc:AT3G57520-MONOMER.

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable galactinol--sucrose galactosyltransferase 2 (EC:2.4.1.82)
    Alternative name(s):
    Protein SEED IMBIBITION 2
    Raffinose synthase 2
    Gene namesi
    Name:RFS2
    Synonyms:RS2, SIP2
    Ordered Locus Names:At3g57520
    ORF Names:T8H10.120
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G57520.

    Subcellular locationi

    GO - Cellular componenti

    1. plasmodesma Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 773773Probable galactinol--sucrose galactosyltransferase 2PRO_0000389256Add
    BLAST

    Proteomic databases

    PaxDbiQ94A08.
    PRIDEiQ94A08.

    Expressioni

    Inductioni

    By oxidative stress.1 Publication

    Gene expression databases

    ArrayExpressiQ94A08.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G57520.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94A08.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Phylogenomic databases

    eggNOGiNOG06986.
    HOGENOMiHOG000237551.
    InParanoidiQ9SCM1.
    KOiK06617.
    OMAiKNEIEIC.
    PhylomeDBiQ94A08.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q94A08-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE    50
    QSKSLHVFPI GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE 100
    SKDEVEGNGD DAPTVYTVFL PLLEGQFRAV LQGNEKNEIE ICFESGDKAV 150
    ETSQGTHLVY VHAGTNPFEV IRQSVKAVER HMQTFHHREK KKLPSFLDWF 200
    GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW QQIENKEKDE 250
    NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK 300
    QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL 350
    AVHGLGLVNP KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR 400
    VSLTRSYQQA LEASIARNFT DNGCISCMCH NTDGLYSAKQ TAIVRASDDF 450
    YPRDPASHTI HIASVAYNSL FLGEFMQPDW DMFHSLHPTA EYHAAARAVG 500
    GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD CLFADPARDG 550
    ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD 600
    DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI 650
    SPLKEITENI SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA 700
    SPALSDNRSP TALVSVSVRG CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV 750
    GLVTLNLPVT REEMFRWHVE ILV 773
    Length:773
    Mass (Da):85,143
    Last modified:November 24, 2009 - v2
    Checksum:i0852F9E67952C8D3
    GO
    Isoform 2 (identifier: Q94A08-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         655-656: EI → DL
         657-773: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:656
    Mass (Da):72,374
    Checksum:i7128B242BF0A1AB1
    GO
    Isoform 3 (identifier: Q94A08-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         552-565: SLLKIWNMNKFTGI → RCWLVQGNEEEPD
         566-773: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:564
    Mass (Da):62,424
    Checksum:i87D74AE3896A5D1A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti255 – 2551Q → R in AAK92707. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei552 – 56514SLLKI…KFTGI → RCWLVQGNEEEPD in isoform 3. CuratedVSP_038412Add
    BLAST
    Alternative sequencei566 – 773208Missing in isoform 3. CuratedVSP_038413Add
    BLAST
    Alternative sequencei655 – 6562EI → DL in isoform 2. CuratedVSP_038414
    Alternative sequencei657 – 773117Missing in isoform 2. CuratedVSP_038415Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL133248 Genomic DNA. Translation: CAB66109.1.
    CP002686 Genomic DNA. Translation: AEE79665.1.
    CP002686 Genomic DNA. Translation: AEE79667.1.
    AY050772 mRNA. Translation: AAK92707.1.
    AK226370 mRNA. Translation: BAE98518.1.
    PIRiT46188.
    RefSeqiNP_191311.1. NM_115612.2. [Q94A08-1]
    NP_850715.1. NM_180384.1. [Q94A08-2]
    UniGeneiAt.22207.
    At.30900.

    Genome annotation databases

    EnsemblPlantsiAT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
    GeneIDi824919.
    KEGGiath:AT3G57520.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL133248 Genomic DNA. Translation: CAB66109.1 .
    CP002686 Genomic DNA. Translation: AEE79665.1 .
    CP002686 Genomic DNA. Translation: AEE79667.1 .
    AY050772 mRNA. Translation: AAK92707.1 .
    AK226370 mRNA. Translation: BAE98518.1 .
    PIRi T46188.
    RefSeqi NP_191311.1. NM_115612.2. [Q94A08-1 ]
    NP_850715.1. NM_180384.1. [Q94A08-2 ]
    UniGenei At.22207.
    At.30900.

    3D structure databases

    ProteinModelPortali Q94A08.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G57520.1-P.

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Proteomic databases

    PaxDbi Q94A08.
    PRIDEi Q94A08.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G57520.1 ; AT3G57520.1 ; AT3G57520 . [Q94A08-1 ]
    GeneIDi 824919.
    KEGGi ath:AT3G57520.

    Organism-specific databases

    TAIRi AT3G57520.

    Phylogenomic databases

    eggNOGi NOG06986.
    HOGENOMi HOG000237551.
    InParanoidi Q9SCM1.
    KOi K06617.
    OMAi KNEIEIC.
    PhylomeDBi Q94A08.

    Enzyme and pathway databases

    BioCyci ARA:AT3G57520-MONOMER.
    ARA:GQT-2348-MONOMER.
    ARA:GQT-2349-MONOMER.
    MetaCyc:AT3G57520-MONOMER.

    Gene expression databases

    ArrayExpressi Q94A08.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
      Nishizawa A., Yabuta Y., Shigeoka S.
      Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiRFS2_ARATH
    AccessioniPrimary (citable) accession number: Q94A08
    Secondary accession number(s): Q0WWI0
    , Q3EAI2, Q3EAI3, Q9SCM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3