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Q94A08

- RFS2_ARATH

UniProt

Q94A08 - RFS2_ARATH

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Protein

Probable galactinol--sucrose galactosyltransferase 2

Gene

RFS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

  1. raffinose alpha-galactosidase activity Source: TAIR
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. raffinose catabolic process Source: TAIR
  2. response to karrikin Source: TAIR
  3. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT3G57520-MONOMER.
ARA:GQT-2348-MONOMER.
ARA:GQT-2349-MONOMER.
MetaCyc:AT3G57520-MONOMER.

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 2 (EC:2.4.1.82)
Alternative name(s):
Protein SEED IMBIBITION 2
Raffinose synthase 2
Gene namesi
Name:RFS2
Synonyms:RS2, SIP2
Ordered Locus Names:At3g57520
ORF Names:T8H10.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G57520.

Subcellular locationi

GO - Cellular componenti

  1. plasmodesma Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773Probable galactinol--sucrose galactosyltransferase 2PRO_0000389256Add
BLAST

Proteomic databases

PaxDbiQ94A08.
PRIDEiQ94A08.

Expressioni

Inductioni

By oxidative stress.1 Publication

Gene expression databases

ExpressionAtlasiQ94A08. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G57520.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ94A08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiNOG06986.
HOGENOMiHOG000237551.
InParanoidiQ94A08.
KOiK06617.
OMAiKNEIEIC.
PhylomeDBiQ94A08.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamiPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q94A08-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE
60 70 80 90 100
QSKSLHVFPI GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE
110 120 130 140 150
SKDEVEGNGD DAPTVYTVFL PLLEGQFRAV LQGNEKNEIE ICFESGDKAV
160 170 180 190 200
ETSQGTHLVY VHAGTNPFEV IRQSVKAVER HMQTFHHREK KKLPSFLDWF
210 220 230 240 250
GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW QQIENKEKDE
260 270 280 290 300
NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK
310 320 330 340 350
QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL
360 370 380 390 400
AVHGLGLVNP KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR
410 420 430 440 450
VSLTRSYQQA LEASIARNFT DNGCISCMCH NTDGLYSAKQ TAIVRASDDF
460 470 480 490 500
YPRDPASHTI HIASVAYNSL FLGEFMQPDW DMFHSLHPTA EYHAAARAVG
510 520 530 540 550
GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD CLFADPARDG
560 570 580 590 600
ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD
610 620 630 640 650
DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI
660 670 680 690 700
SPLKEITENI SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA
710 720 730 740 750
SPALSDNRSP TALVSVSVRG CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV
760 770
GLVTLNLPVT REEMFRWHVE ILV
Length:773
Mass (Da):85,143
Last modified:November 24, 2009 - v2
Checksum:i0852F9E67952C8D3
GO
Isoform 2 (identifier: Q94A08-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     655-656: EI → DL
     657-773: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:656
Mass (Da):72,374
Checksum:i7128B242BF0A1AB1
GO
Isoform 3 (identifier: Q94A08-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     552-565: SLLKIWNMNKFTGI → RCWLVQGNEEEPD
     566-773: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:564
Mass (Da):62,424
Checksum:i87D74AE3896A5D1A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551Q → R in AAK92707. (PubMed:14593172)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei552 – 56514SLLKI…KFTGI → RCWLVQGNEEEPD in isoform 3. CuratedVSP_038412Add
BLAST
Alternative sequencei566 – 773208Missing in isoform 3. CuratedVSP_038413Add
BLAST
Alternative sequencei655 – 6562EI → DL in isoform 2. CuratedVSP_038414
Alternative sequencei657 – 773117Missing in isoform 2. CuratedVSP_038415Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133248 Genomic DNA. Translation: CAB66109.1.
CP002686 Genomic DNA. Translation: AEE79665.1.
CP002686 Genomic DNA. Translation: AEE79667.1.
AY050772 mRNA. Translation: AAK92707.1.
AK226370 mRNA. Translation: BAE98518.1.
PIRiT46188.
RefSeqiNP_191311.1. NM_115612.2. [Q94A08-1]
NP_850715.1. NM_180384.1. [Q94A08-2]
UniGeneiAt.22207.
At.30900.

Genome annotation databases

EnsemblPlantsiAT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
GeneIDi824919.
KEGGiath:AT3G57520.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133248 Genomic DNA. Translation: CAB66109.1 .
CP002686 Genomic DNA. Translation: AEE79665.1 .
CP002686 Genomic DNA. Translation: AEE79667.1 .
AY050772 mRNA. Translation: AAK92707.1 .
AK226370 mRNA. Translation: BAE98518.1 .
PIRi T46188.
RefSeqi NP_191311.1. NM_115612.2. [Q94A08-1 ]
NP_850715.1. NM_180384.1. [Q94A08-2 ]
UniGenei At.22207.
At.30900.

3D structure databases

ProteinModelPortali Q94A08.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G57520.1-P.

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbi Q94A08.
PRIDEi Q94A08.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G57520.1 ; AT3G57520.1 ; AT3G57520 . [Q94A08-1 ]
GeneIDi 824919.
KEGGi ath:AT3G57520.

Organism-specific databases

TAIRi AT3G57520.

Phylogenomic databases

eggNOGi NOG06986.
HOGENOMi HOG000237551.
InParanoidi Q94A08.
KOi K06617.
OMAi KNEIEIC.
PhylomeDBi Q94A08.

Enzyme and pathway databases

BioCyci ARA:AT3G57520-MONOMER.
ARA:GQT-2348-MONOMER.
ARA:GQT-2349-MONOMER.
MetaCyc:AT3G57520-MONOMER.

Gene expression databases

ExpressionAtlasi Q94A08. baseline and differential.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view ]
Pfami PF05691. Raffinose_syn. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
    Nishizawa A., Yabuta Y., Shigeoka S.
    Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiRFS2_ARATH
AccessioniPrimary (citable) accession number: Q94A08
Secondary accession number(s): Q0WWI0
, Q3EAI2, Q3EAI3, Q9SCM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 24, 2009
Last modified: October 29, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3