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Protein

Mitogen-activated protein kinase kinase 1

Gene

MKK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, abscisic acid (ABA), hydrogen peroxide, bacterial flagellin and laminarin beta-glucan.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971ATPPROSITE-ProRule annotation
Active sitei190 – 1901Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 829ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • defense response, incompatible interaction Source: TAIR
  • defense response to bacterium Source: TAIR
  • response to hydrogen peroxide Source: TAIR
  • response to molecule of bacterial origin Source: TAIR
  • response to water deprivation Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Immunity, Innate immunity, Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G26070-MONOMER.
ARA:GQT-1678-MONOMER.
ARA:GQT-1679-MONOMER.
BRENDAi2.7.12.2. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 1 (EC:2.7.12.2)
Short name:
AtMKK1
Short name:
MAP kinase kinase 1
Alternative name(s):
AtMEK1
NMAPKK
Gene namesi
Name:MKK1
Synonyms:MEK1
Ordered Locus Names:At4g26070
ORF Names:F20B18.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26070.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No obvious developmental defects under normal growth conditions. Compromised in resistance to both virulent and avirulent Pseudomonas syringae strains. Reduced sensitivity to abscisic acid (ABA) during germination and reduced drought tolerance of seedlings. Simultaneous knockdown of MKK1 and MKK2 results in dwarf and small plants exhibiting a seedling-lethality phenotype.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi97 – 971K → R: Loss of kinase activity in vitro. 1 Publication
Mutagenesisi218 – 2181T → A: Loss of kinase activity in vitro; when associated with E-224. 3 Publications
Mutagenesisi218 – 2181T → E: Constitutively active; when associated with D-224. Increases kinase activity in vitro; when associated with E-224. 3 Publications
Mutagenesisi220 – 2201S → E: Increases kinase activity in vitro; when associated with E-224. 1 Publication
Mutagenesisi224 – 2241S → D: Constitutively active; when associated with E-218. 3 Publications
Mutagenesisi224 – 2241S → E: Increases kinase activity in vitro; when associated with E-218 or E-220. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Mitogen-activated protein kinase kinase 1PRO_0000245821Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181Phosphothreonine3 Publications
Modified residuei224 – 2241Phosphoserine2 Publications
Modified residuei228 – 2281PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation at Thr-218 and Ser-224 by MAP kinase kinase kinases positively regulates kinase activity.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ94A06.
PRIDEiQ94A06.

PTM databases

iPTMnetiQ94A06.

Expressioni

Tissue specificityi

Expressed in roots, stem, flowers and siliques.1 Publication

Inductioni

By wounding.1 Publication

Gene expression databases

GenevisibleiQ94A06. AT.

Interactioni

Subunit structurei

Interacts with MEKK1 and MPK4. May form a ternary complex composed of MEKK1 and MKK1/MKK2 and MPK4. Interacts with P.syringae type III effector HopF2. Interacts with MPK11.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MEKK1Q390084EBI-994464,EBI-994439
MPK11Q9LMM52EBI-994464,EBI-2358699
MPK12Q8GYQ52EBI-994464,EBI-2128461
MPK4Q390246EBI-994464,EBI-994375

Protein-protein interaction databases

BioGridi14000. 2 interactions.
IntActiQ94A06. 4 interactions.
STRINGi3702.AT4G26070.2.

Structurei

3D structure databases

ProteinModelPortaliQ94A06.
SMRiQ94A06. Positions 62-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 328261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ94A06.
OMAiHVEANIN.
OrthoDBiEOG09360FLW.
PhylomeDBiQ94A06.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q94A06-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRGSLCPNP ICLPPLEQSI SKFLTQSGTF KDGDLRVNKD GIQTVSLSEP
60 70 80 90 100
GAPPPIEPLD NQLSLADLEV IKVIGKGSSG NVQLVKHKLT QQFFALKVIQ
110 120 130 140 150
LNTEESTCRA ISQELRINLS SQCPYLVSCY QSFYHNGLVS IILEFMDGGS
160 170 180 190 200
LADLLKKVGK VPENMLSAIC KRVLRGLCYI HHERRIIHRD LKPSNLLINH
210 220 230 240 250
RGEVKITDFG VSKILTSTSS LANSFVGTYP YMSPERISGS LYSNKSDIWS
260 270 280 290 300
LGLVLLECAT GKFPYTPPEH KKGWSSVYEL VDAIVENPPP CAPSNLFSPE
310 320 330 340 350
FCSFISQCVQ KDPRDRKSAK ELLEHKFVKM FEDSDTNLSA YFTDAGSLIP

PLAN
Length:354
Mass (Da):39,210
Last modified:July 11, 2006 - v2
Checksum:iD889CFBB97653B8E
GO
Isoform 2 (identifier: Q94A06-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-308: C → W
     309-354: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:308
Mass (Da):34,087
Checksum:i76E772A778D66F5E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei308 – 3081C → W in isoform 2. 1 PublicationVSP_019782
Alternative sequencei309 – 35446Missing in isoform 2. 1 PublicationVSP_019783Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000977 mRNA. Translation: AAB97145.1.
AB004796 mRNA. Translation: BAA24079.1.
AL049483 Genomic DNA. Translation: CAB39672.1.
AL161564 Genomic DNA. Translation: CAB79462.1.
CP002687 Genomic DNA. Translation: AEE85152.1.
CP002687 Genomic DNA. Translation: AEE85153.1.
CP002687 Genomic DNA. Translation: AEE85154.1.
AY050774 mRNA. Translation: AAK92709.1.
BT001935 mRNA. Translation: AAN71934.1.
AY087065 mRNA. Translation: AAM64626.1.
PIRiT04262.
RefSeqiNP_194337.1. NM_118740.2. [Q94A06-1]
NP_849446.1. NM_179115.1. [Q94A06-2]
NP_974619.1. NM_202890.1. [Q94A06-1]
UniGeneiAt.21332.
At.223.

Genome annotation databases

EnsemblPlantsiAT4G26070.2; AT4G26070.2; AT4G26070. [Q94A06-1]
AT4G26070.3; AT4G26070.3; AT4G26070. [Q94A06-1]
GeneIDi828713.
KEGGiath:AT4G26070.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000977 mRNA. Translation: AAB97145.1.
AB004796 mRNA. Translation: BAA24079.1.
AL049483 Genomic DNA. Translation: CAB39672.1.
AL161564 Genomic DNA. Translation: CAB79462.1.
CP002687 Genomic DNA. Translation: AEE85152.1.
CP002687 Genomic DNA. Translation: AEE85153.1.
CP002687 Genomic DNA. Translation: AEE85154.1.
AY050774 mRNA. Translation: AAK92709.1.
BT001935 mRNA. Translation: AAN71934.1.
AY087065 mRNA. Translation: AAM64626.1.
PIRiT04262.
RefSeqiNP_194337.1. NM_118740.2. [Q94A06-1]
NP_849446.1. NM_179115.1. [Q94A06-2]
NP_974619.1. NM_202890.1. [Q94A06-1]
UniGeneiAt.21332.
At.223.

3D structure databases

ProteinModelPortaliQ94A06.
SMRiQ94A06. Positions 62-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14000. 2 interactions.
IntActiQ94A06. 4 interactions.
STRINGi3702.AT4G26070.2.

PTM databases

iPTMnetiQ94A06.

Proteomic databases

PaxDbiQ94A06.
PRIDEiQ94A06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26070.2; AT4G26070.2; AT4G26070. [Q94A06-1]
AT4G26070.3; AT4G26070.3; AT4G26070. [Q94A06-1]
GeneIDi828713.
KEGGiath:AT4G26070.

Organism-specific databases

TAIRiAT4G26070.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ94A06.
OMAiHVEANIN.
OrthoDBiEOG09360FLW.
PhylomeDBiQ94A06.

Enzyme and pathway databases

BioCyciARA:AT4G26070-MONOMER.
ARA:GQT-1678-MONOMER.
ARA:GQT-1679-MONOMER.
BRENDAi2.7.12.2. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Miscellaneous databases

PROiQ94A06.

Gene expression databases

GenevisibleiQ94A06. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM2K1_ARATH
AccessioniPrimary (citable) accession number: Q94A06
Secondary accession number(s): O04440, O04661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: September 7, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.