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Protein

Mitogen-activated protein kinase kinase 1

Gene

MKK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, abscisic acid (ABA), hydrogen peroxide, bacterial flagellin and laminarin beta-glucan.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei190Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • defense response, incompatible interaction Source: TAIR
  • defense response to bacterium Source: TAIR
  • regulation of mitotic cell cycle Source: GO_Central
  • response to hydrogen peroxide Source: TAIR
  • response to molecule of bacterial origin Source: TAIR
  • response to water deprivation Source: TAIR
  • response to wounding Source: TAIR
  • stress-activated protein kinase signaling cascade Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processImmunity, Innate immunity, Plant defense, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2 399
ReactomeiR-ATH-110056 MAPK3 (ERK1) activation
R-ATH-2559580 Oxidative Stress Induced Senescence
R-ATH-2871796 FCERI mediated MAPK activation
R-ATH-450302 activated TAK1 mediates p38 MAPK activation
R-ATH-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 1 (EC:2.7.12.2)
Short name:
AtMKK1
Short name:
MAP kinase kinase 1
Alternative name(s):
AtMEK1
NMAPKK
Gene namesi
Name:MKK1
Synonyms:MEK1
Ordered Locus Names:At4g26070
ORF Names:F20B18.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26070
TAIRilocus:2120855 AT4G26070

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

No obvious developmental defects under normal growth conditions. Compromised in resistance to both virulent and avirulent Pseudomonas syringae strains. Reduced sensitivity to abscisic acid (ABA) during germination and reduced drought tolerance of seedlings. Simultaneous knockdown of MKK1 and MKK2 results in dwarf and small plants exhibiting a seedling-lethality phenotype.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97K → R: Loss of kinase activity in vitro. 1 Publication1
Mutagenesisi218T → A: Loss of kinase activity in vitro; when associated with E-224. 3 Publications1
Mutagenesisi218T → E: Constitutively active; when associated with D-224. Increases kinase activity in vitro; when associated with E-224. 3 Publications1
Mutagenesisi220S → E: Increases kinase activity in vitro; when associated with E-224. 1 Publication1
Mutagenesisi224S → D: Constitutively active; when associated with E-218. 3 Publications1
Mutagenesisi224S → E: Increases kinase activity in vitro; when associated with E-218 or E-220. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458211 – 354Mitogen-activated protein kinase kinase 1Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218Phosphothreonine3 Publications1
Modified residuei224Phosphoserine2 Publications1
Modified residuei228PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Thr-218 and Ser-224 by MAP kinase kinase kinases positively regulates kinase activity.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ94A06
PRIDEiQ94A06

PTM databases

iPTMnetiQ94A06

Expressioni

Tissue specificityi

Expressed in roots, stem, flowers and siliques.1 Publication

Inductioni

By wounding.1 Publication

Gene expression databases

ExpressionAtlasiQ94A06 baseline and differential
GenevisibleiQ94A06 AT

Interactioni

Subunit structurei

Interacts with MEKK1 and MPK4. May form a ternary complex composed of MEKK1 and MKK1/MKK2 and MPK4. Interacts with P.syringae type III effector HopF2. Interacts with MPK11.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi14000, 2 interactors
IntActiQ94A06, 4 interactors
STRINGi3702.AT4G26070.2

Structurei

3D structure databases

ProteinModelPortaliQ94A06
SMRiQ94A06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 328Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0581 Eukaryota
ENOG410XQ5A LUCA
HOGENOMiHOG000234206
InParanoidiQ94A06
KOiK20602
OMAiVMKSNDC
OrthoDBiEOG09360FLW
PhylomeDBiQ94A06

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q94A06-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRGSLCPNP ICLPPLEQSI SKFLTQSGTF KDGDLRVNKD GIQTVSLSEP
60 70 80 90 100
GAPPPIEPLD NQLSLADLEV IKVIGKGSSG NVQLVKHKLT QQFFALKVIQ
110 120 130 140 150
LNTEESTCRA ISQELRINLS SQCPYLVSCY QSFYHNGLVS IILEFMDGGS
160 170 180 190 200
LADLLKKVGK VPENMLSAIC KRVLRGLCYI HHERRIIHRD LKPSNLLINH
210 220 230 240 250
RGEVKITDFG VSKILTSTSS LANSFVGTYP YMSPERISGS LYSNKSDIWS
260 270 280 290 300
LGLVLLECAT GKFPYTPPEH KKGWSSVYEL VDAIVENPPP CAPSNLFSPE
310 320 330 340 350
FCSFISQCVQ KDPRDRKSAK ELLEHKFVKM FEDSDTNLSA YFTDAGSLIP

PLAN
Length:354
Mass (Da):39,210
Last modified:July 11, 2006 - v2
Checksum:iD889CFBB97653B8E
GO
Isoform 2 (identifier: Q94A06-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-308: C → W
     309-354: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:308
Mass (Da):34,087
Checksum:i76E772A778D66F5E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019782308C → W in isoform 2. 1 Publication1
Alternative sequenceiVSP_019783309 – 354Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000977 mRNA Translation: AAB97145.1
AB004796 mRNA Translation: BAA24079.1
AL049483 Genomic DNA Translation: CAB39672.1
AL161564 Genomic DNA Translation: CAB79462.1
CP002687 Genomic DNA Translation: AEE85152.1
CP002687 Genomic DNA Translation: AEE85153.1
CP002687 Genomic DNA Translation: AEE85154.1
AY050774 mRNA Translation: AAK92709.1
BT001935 mRNA Translation: AAN71934.1
AY087065 mRNA Translation: AAM64626.1
PIRiT04262
RefSeqiNP_194337.1, NM_118740.3 [Q94A06-1]
NP_849446.1, NM_179115.2 [Q94A06-2]
NP_974619.1, NM_202890.2 [Q94A06-1]
UniGeneiAt.21332
At.223

Genome annotation databases

EnsemblPlantsiAT4G26070.1; AT4G26070.1; AT4G26070 [Q94A06-2]
AT4G26070.2; AT4G26070.2; AT4G26070 [Q94A06-1]
AT4G26070.3; AT4G26070.3; AT4G26070 [Q94A06-1]
GeneIDi828713
GrameneiAT4G26070.1; AT4G26070.1; AT4G26070 [Q94A06-2]
AT4G26070.2; AT4G26070.2; AT4G26070 [Q94A06-1]
AT4G26070.3; AT4G26070.3; AT4G26070 [Q94A06-1]
KEGGiath:AT4G26070

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiM2K1_ARATH
AccessioniPrimary (citable) accession number: Q94A06
Secondary accession number(s): O04440, O04661
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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