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Q94A02

- TAR2_ARATH

UniProt

Q94A02 - TAR2_ARATH

Protein

Tryptophan aminotransferase-related protein 2

Gene

TAR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Involved in auxin production. Both TAA1 and TAR2 are required for maintaining proper auxin levels in roots, while TAA1, TAR1 and TAR2 are required for proper embryo patterning. Involved in the maintenance of the root stem cell niches.1 Publication

    Catalytic activityi

    L-tryptophan + 2-oxoglutarate = (indol-3-yl)pyruvate + L-glutamate.
    L-tryptophan + pyruvate = indole-3-pyruvate + L-alanine.

    Cofactori

    Pyridoxal phosphate.By similarity

    Enzyme regulationi

    Inhibited by L-kynurenine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei112 – 1121Pyridoxal phosphateBy similarity
    Binding sitei222 – 2221Pyridoxal phosphateBy similarity
    Binding sitei242 – 2421Pyridoxal phosphateBy similarity
    Binding sitei276 – 2761Pyridoxal phosphateBy similarity

    GO - Molecular functioni

    1. carbon-sulfur lyase activity Source: InterPro
    2. L-tryptophan:2-oxoglutarate aminotransferase activity Source: TAIR
    3. L-tryptophan:pyruvate aminotransferase activity Source: TAIR
    4. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cotyledon development Source: TAIR
    2. cotyledon vascular tissue pattern formation Source: TAIR
    3. defense response to bacterium Source: TAIR
    4. embryo development ending in seed dormancy Source: TAIR
    5. flower development Source: TAIR
    6. gynoecium development Source: TAIR
    7. indoleacetic acid biosynthetic process Source: TAIR
    8. maintenance of root meristem identity Source: TAIR
    9. phloem or xylem histogenesis Source: TAIR
    10. positive gravitropism Source: TAIR
    11. primary root development Source: TAIR
    12. response to ethylene Source: TAIR
    13. shoot system development Source: TAIR

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Auxin biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:GQT-2242-MONOMER.
    UniPathwayiUPA00151.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan aminotransferase-related protein 2 (EC:2.6.1.27, EC:2.6.1.99)
    Gene namesi
    Name:TAR2
    Ordered Locus Names:At4g24670
    ORF Names:F22K18.130
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G24670.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Tryptophan aminotransferase-related protein 2PRO_0000411675Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei268 – 2681N6-(pyridoxal phosphate)lysineSequence Analysis

    Proteomic databases

    PaxDbiQ94A02.
    PRIDEiQ94A02.

    Expressioni

    Tissue specificityi

    Expressed in roots, cotyledons and in the apical parts of hypocotyls. In roots, restricted to the provasculature of meristematic regions. Detected on the inner side of the apical hooks.1 Publication

    Developmental stagei

    In early-stage flowers, expressed in all floral organs. Becomes later restricted to the gynoecia, preferentially to the outer cell layers that give rise to the valves. No expression in flowers at anthesis.1 Publication

    Inductioni

    Up-regulated by ethylene.1 Publication

    Gene expression databases

    GenevestigatoriQ94A02.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G24670.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ94A02.
    SMRiQ94A02. Positions 76-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2620HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni154 – 1552Pyridoxal phosphate bindingBy similarity
    Regioni242 – 2454Pyridoxal phosphate bindingBy similarity
    Regioni265 – 2684Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the alliinase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG331832.
    HOGENOMiHOG000237549.
    InParanoidiQ94A02.
    KOiK16903.
    OMAiGWQSMSY.
    PhylomeDBiQ94A02.

    Family and domain databases

    Gene3Di2.10.25.30. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR006948. Alliinase_C.
    IPR006947. EGF_alliinase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF04864. Alliinase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q94A02-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQIPRFLSW RNMLVLSLAI NFSLILKILK GDRERGDSWD RTAYVSIWPV    50
    VSTTASESSS LSSASCNYSK IEEDDDRIIN LKFGDPTVYE RYWQENGEVT 100
    TMVIPGWQSL SYFSDENNLC WFLEPELAKE IVRVHKVVGN AVTQDRFIVV 150
    GTGSTQLYQA ALYALSPHDD SGPINVVSAT PYYSTYPLIT DCLKSGLYRW 200
    GGDAKTYKED GPYIELVTSP NNPDGFLRES VVNSTEGILI HDLAYYWPQY 250
    TPITSPADHD VMLFTASKST GHAGIRIGWA LVKDRETARK MIEYIELNTI 300
    GVSKDSQLRV AKVLKVVSDS CGNVTGKSFF DHSYDAMYER WKLLKQAAKD 350
    TKRFSVPDFV SQRCNFFGRV FEPQPAFAWF KCEEGIVDCE KFLREEKKIL 400
    TKSGKYFGDE LSNVRISMLD RDTNFNIFLH RITSSFNSTL 440
    Length:440
    Mass (Da):50,007
    Last modified:December 1, 2001 - v1
    Checksum:i1FED7264F48B1D87
    GO

    Sequence cautioni

    The sequence CAA22996.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB79377.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035356 Genomic DNA. Translation: CAA22996.1. Sequence problems.
    AL161561 Genomic DNA. Translation: CAB79377.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84940.1.
    CP002687 Genomic DNA. Translation: AEE84941.1.
    AY050779 mRNA. Translation: AAK92714.1.
    AY091455 mRNA. Translation: AAM14394.1.
    PIRiT05567.
    RefSeqiNP_567706.1. NM_118600.1.
    NP_974608.1. NM_202879.1.
    UniGeneiAt.26567.

    Genome annotation databases

    EnsemblPlantsiAT4G24670.1; AT4G24670.1; AT4G24670.
    AT4G24670.2; AT4G24670.2; AT4G24670.
    GeneIDi828569.
    KEGGiath:AT4G24670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035356 Genomic DNA. Translation: CAA22996.1 . Sequence problems.
    AL161561 Genomic DNA. Translation: CAB79377.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84940.1 .
    CP002687 Genomic DNA. Translation: AEE84941.1 .
    AY050779 mRNA. Translation: AAK92714.1 .
    AY091455 mRNA. Translation: AAM14394.1 .
    PIRi T05567.
    RefSeqi NP_567706.1. NM_118600.1.
    NP_974608.1. NM_202879.1.
    UniGenei At.26567.

    3D structure databases

    ProteinModelPortali Q94A02.
    SMRi Q94A02. Positions 76-434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G24670.2-P.

    Proteomic databases

    PaxDbi Q94A02.
    PRIDEi Q94A02.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G24670.1 ; AT4G24670.1 ; AT4G24670 .
    AT4G24670.2 ; AT4G24670.2 ; AT4G24670 .
    GeneIDi 828569.
    KEGGi ath:AT4G24670.

    Organism-specific databases

    TAIRi AT4G24670.

    Phylogenomic databases

    eggNOGi NOG331832.
    HOGENOMi HOG000237549.
    InParanoidi Q94A02.
    KOi K16903.
    OMAi GWQSMSY.
    PhylomeDBi Q94A02.

    Enzyme and pathway databases

    UniPathwayi UPA00151 .
    BioCyci ARA:GQT-2242-MONOMER.

    Gene expression databases

    Genevestigatori Q94A02.

    Family and domain databases

    Gene3Di 2.10.25.30. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR006948. Alliinase_C.
    IPR006947. EGF_alliinase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF04864. Alliinase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development."
      Stepanova A.N., Robertson-Hoyt J., Yun J., Benavente L.M., Xie D.Y., Dolezal K., Schlereth A., Juergens G., Alonso J.M.
      Cell 133:177-191(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY ETHYLENE, GENE FAMILY, NOMENCLATURE.
    5. "Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants."
      Tao Y., Ferrer J.L., Ljung K., Pojer F., Hong F., Long J.A., Li L., Moreno J.E., Bowman M.E., Ivans L.J., Cheng Y., Lim J., Zhao Y., Ballare C.L., Sandberg G., Noel J.P., Chory J.
      Cell 133:164-176(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis."
      He W., Brumos J., Li H., Ji Y., Ke M., Gong X., Zeng Q., Li W., Zhang X., An F., Wen X., Li P., Chu J., Sun X., Yan C., Yan N., Xie D.Y., Raikhel N.
      , Yang Z., Stepanova A.N., Alonso J.M., Guo H.
      Plant Cell 23:3944-3960(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.

    Entry informationi

    Entry nameiTAR2_ARATH
    AccessioniPrimary (citable) accession number: Q94A02
    Secondary accession number(s): Q9SB62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3