Q949Z1 (PGLR4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase At1g48100
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase At1g48100

Gene names

Ordered Locus Names:	At1g48100
ORF Names:	F21D18.17, F21D18.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polygalacturonase not involved in the final stages of pod shatter. Ref.5
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in flowers and stems. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.
Sequence caution	The sequence AAF79535.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 475	448	Polygalacturonase At1g48100		PRO_0000367916
Regions
	Repeat	241 – 267	27	PbH1 1
	Repeat	268 – 289	22	PbH1 2
	Repeat	291 – 311	21	PbH1 3
	Repeat	321 – 342	22	PbH1 4
	Repeat	350 – 371	22	PbH1 5
	Compositional bias	41 – 71	31	Pro-rich
	Compositional bias	201 – 204	4	Poly-Ser
Sites
	Active site	282	1	Proton donor By similarity
	Active site	305	1	By similarity
Experimental info
	Sequence conflict	123	1	K → R in AAK95305. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q949Z1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: ACB066CF725F2C8F	FASTA	475	51,353
	10 20 30 40 50 60 MRRLSGILVI YIISTVLFGH FTNVARARYH YHKGRHGVTH PLPPPPPPPL ETANPPDQVP 70 80 90 100 110 120 SDPYPSPDPA PGDSDSGCVF DVTSFGAVGD GSCDDTAAFQ DAWKAACAVE SGVVLAPEGG 130 140 150 160 170 180 VFKITSTIFS GPCKPGLVFQ LDGVLMPPDG PEEWPEKDNK NQWLVFYRLD GFTFSGKGTV 190 200 210 220 230 240 EGNGQKWWDL PCKPHRGPDG SSSSGPCASP TMIRFFMSNN IEVKGLRIQN SPQFHMKFDG 250 260 270 280 290 300 CQGVLINEIQ ISSPKLSPNT DGIHLGNTRS VGIYNSVVSN GDDCISIGTG CSDVDIQGVT 310 320 330 340 350 360 CGPSHGISIG SLGVHNSQAC VSNITVRNTV IRDSDNGLRV KTWQGGTGSV SNLLFENIQM 370 380 390 400 410 420 ENVLNCIIVD QYYCQSKDCR NETSAVKVFD VEYRNIKGTY DVRSPPIHFA CSDTVACTNI 430 440 450 460 470 TMSEVELLPE EGELVDDPFC WNAYGKQETL TIPPIDCLLD GSPVVEEAYD SNYGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Patterns of expansion and expression divergence in the plant polygalacturonase gene family." Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E. Genome Biol. 7:R87.1-R87.14(2006) [PubMed: 17010199] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are polygalacturonases required for cell separation during reproductive development in Arabidopsis." Ogawa M., Kay P., Wilson S., Swain S.M. Plant Cell 21:216-233(2009) [PubMed: 19168715] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC023673 Genomic DNA. Translation: AAF79535.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE32248.1. AF410319 mRNA. Translation: AAK95305.1. AY050798 mRNA. Translation: AAK92733.1. AY096360 mRNA. Translation: AAM20001.1.
IPI	IPI00541837.
PIR	C96521.
RefSeq	NP_175244.1. NM_103706.3.
UniGene	At.48286. At.70184.
3D structure databases
ProteinModelPortal	Q949Z1.
SMR	Q949Z1. Positions 74-129.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	Q949Z1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G48100.1; AT1G48100.1; AT1G48100.
GeneID	841228.
GenomeReviews	Gene locus AT1G48100 in contig CT485782_GR.
KEGG	ath:AT1G48100.
NMPDR	fig|3702.1.peg.4273.
Organism-specific databases
GeneFarm	478. 2.
TAIR	At1g48100.
Phylogenomic databases
eggNOG	euNOG04659.
GeneTree	EPGT00070000028128.
InParanoid	Q949Z1.
OMA	PCKPGLV.
PhylomeDB	Q949Z1.
ProtClustDB	CLSN2682184.
Gene expression databases
ArrayExpress	Q949Z1.
Genevestigator	Q949Z1.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR4_ARATH

Accession

Primary (citable) accession number: Q949Z1
Secondary accession number(s): Q94EH4, Q9LNG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program