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Protein

Polygalacturonase At1g48100

Gene

At1g48100

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Polygalacturonase not involved in the final stages of pod shatter.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei282Proton donorPROSITE-ProRule annotation1
Active sitei305PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G48100-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase At1g48100 (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Pectinase At1g48100
Gene namesi
Ordered Locus Names:At1g48100
ORF Names:F21D18.17, F21D18.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G48100.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000036791628 – 475Polygalacturonase At1g48100Add BLAST448

Proteomic databases

PaxDbiQ949Z1.

Expressioni

Tissue specificityi

Expressed in flowers and stems.1 Publication

Gene expression databases

GenevisibleiQ949Z1. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G48100.1.

Structurei

3D structure databases

ProteinModelPortaliQ949Z1.
SMRiQ949Z1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati241 – 267PbH1 1Add BLAST27
Repeati268 – 289PbH1 2Add BLAST22
Repeati291 – 311PbH1 3Add BLAST21
Repeati321 – 342PbH1 4Add BLAST22
Repeati350 – 371PbH1 5Add BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 71Pro-richAdd BLAST31
Compositional biasi201 – 204Poly-Ser4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IH0Q. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ949Z1.
OMAiIRFFMSN.
OrthoDBiEOG0936091F.
PhylomeDBiQ949Z1.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q949Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRLSGILVI YIISTVLFGH FTNVARARYH YHKGRHGVTH PLPPPPPPPL
60 70 80 90 100
ETANPPDQVP SDPYPSPDPA PGDSDSGCVF DVTSFGAVGD GSCDDTAAFQ
110 120 130 140 150
DAWKAACAVE SGVVLAPEGG VFKITSTIFS GPCKPGLVFQ LDGVLMPPDG
160 170 180 190 200
PEEWPEKDNK NQWLVFYRLD GFTFSGKGTV EGNGQKWWDL PCKPHRGPDG
210 220 230 240 250
SSSSGPCASP TMIRFFMSNN IEVKGLRIQN SPQFHMKFDG CQGVLINEIQ
260 270 280 290 300
ISSPKLSPNT DGIHLGNTRS VGIYNSVVSN GDDCISIGTG CSDVDIQGVT
310 320 330 340 350
CGPSHGISIG SLGVHNSQAC VSNITVRNTV IRDSDNGLRV KTWQGGTGSV
360 370 380 390 400
SNLLFENIQM ENVLNCIIVD QYYCQSKDCR NETSAVKVFD VEYRNIKGTY
410 420 430 440 450
DVRSPPIHFA CSDTVACTNI TMSEVELLPE EGELVDDPFC WNAYGKQETL
460 470
TIPPIDCLLD GSPVVEEAYD SNYGC
Length:475
Mass (Da):51,353
Last modified:December 1, 2001 - v1
Checksum:iACB066CF725F2C8F
GO

Sequence cautioni

The sequence AAF79535 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123K → R in AAK95305 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC023673 Genomic DNA. Translation: AAF79535.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32248.1.
AF410319 mRNA. Translation: AAK95305.1.
AY050798 mRNA. Translation: AAK92733.1.
AY096360 mRNA. Translation: AAM20001.1.
PIRiC96521.
RefSeqiNP_175244.1. NM_103706.4.
UniGeneiAt.48286.
At.70184.

Genome annotation databases

EnsemblPlantsiAT1G48100.1; AT1G48100.1; AT1G48100.
GeneIDi841228.
GrameneiAT1G48100.1; AT1G48100.1; AT1G48100.
KEGGiath:AT1G48100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC023673 Genomic DNA. Translation: AAF79535.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32248.1.
AF410319 mRNA. Translation: AAK95305.1.
AY050798 mRNA. Translation: AAK92733.1.
AY096360 mRNA. Translation: AAM20001.1.
PIRiC96521.
RefSeqiNP_175244.1. NM_103706.4.
UniGeneiAt.48286.
At.70184.

3D structure databases

ProteinModelPortaliQ949Z1.
SMRiQ949Z1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G48100.1.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PaxDbiQ949Z1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G48100.1; AT1G48100.1; AT1G48100.
GeneIDi841228.
GrameneiAT1G48100.1; AT1G48100.1; AT1G48100.
KEGGiath:AT1G48100.

Organism-specific databases

TAIRiAT1G48100.

Phylogenomic databases

eggNOGiENOG410IH0Q. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ949Z1.
OMAiIRFFMSN.
OrthoDBiEOG0936091F.
PhylomeDBiQ949Z1.

Enzyme and pathway databases

BioCyciARA:AT1G48100-MONOMER.

Miscellaneous databases

PROiQ949Z1.

Gene expression databases

GenevisibleiQ949Z1. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR4_ARATH
AccessioniPrimary (citable) accession number: Q949Z1
Secondary accession number(s): Q94EH4, Q9LNG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.