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Protein

Polygalacturonase At1g48100

Gene

At1g48100

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Polygalacturonase not involved in the final stages of pod shatter.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei282Proton donorPROSITE-ProRule annotation1
Active sitei305PROSITE-ProRule annotation1

GO - Molecular functioni

  • polygalacturonase activity Source: TAIR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • plant-type cell wall modification involved in multidimensional cell growth Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G48100-MONOMER

Protein family/group databases

CAZyiGH28 Glycoside Hydrolase Family 28

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase At1g48100 (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Pectinase At1g48100
Gene namesi
Ordered Locus Names:At1g48100
ORF Names:F21D18.17, F21D18.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G48100
TAIRilocus:2023817 AT1G48100

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000036791628 – 475Polygalacturonase At1g48100Add BLAST448

Proteomic databases

PaxDbiQ949Z1
PRIDEiQ949Z1

Expressioni

Tissue specificityi

Expressed in flowers and stems.1 Publication

Gene expression databases

ExpressionAtlasiQ949Z1 baseline and differential
GenevisibleiQ949Z1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G48100.1

Structurei

3D structure databases

ProteinModelPortaliQ949Z1
SMRiQ949Z1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati241 – 267PbH1 1Add BLAST27
Repeati268 – 289PbH1 2Add BLAST22
Repeati291 – 311PbH1 3Add BLAST21
Repeati321 – 342PbH1 4Add BLAST22
Repeati350 – 371PbH1 5Add BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 71Pro-richAdd BLAST31
Compositional biasi201 – 204Poly-Ser4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IH0Q Eukaryota
COG5434 LUCA
HOGENOMiHOG000237792
InParanoidiQ949Z1
OMAiSKDCRNE
OrthoDBiEOG0936091F
PhylomeDBiQ949Z1

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR000743 Glyco_hydro_28
IPR006626 PbH1
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
PfamiView protein in Pfam
PF00295 Glyco_hydro_28, 1 hit
SMARTiView protein in SMART
SM00710 PbH1, 5 hits
SUPFAMiSSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00502 POLYGALACTURONASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q949Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRLSGILVI YIISTVLFGH FTNVARARYH YHKGRHGVTH PLPPPPPPPL
60 70 80 90 100
ETANPPDQVP SDPYPSPDPA PGDSDSGCVF DVTSFGAVGD GSCDDTAAFQ
110 120 130 140 150
DAWKAACAVE SGVVLAPEGG VFKITSTIFS GPCKPGLVFQ LDGVLMPPDG
160 170 180 190 200
PEEWPEKDNK NQWLVFYRLD GFTFSGKGTV EGNGQKWWDL PCKPHRGPDG
210 220 230 240 250
SSSSGPCASP TMIRFFMSNN IEVKGLRIQN SPQFHMKFDG CQGVLINEIQ
260 270 280 290 300
ISSPKLSPNT DGIHLGNTRS VGIYNSVVSN GDDCISIGTG CSDVDIQGVT
310 320 330 340 350
CGPSHGISIG SLGVHNSQAC VSNITVRNTV IRDSDNGLRV KTWQGGTGSV
360 370 380 390 400
SNLLFENIQM ENVLNCIIVD QYYCQSKDCR NETSAVKVFD VEYRNIKGTY
410 420 430 440 450
DVRSPPIHFA CSDTVACTNI TMSEVELLPE EGELVDDPFC WNAYGKQETL
460 470
TIPPIDCLLD GSPVVEEAYD SNYGC
Length:475
Mass (Da):51,353
Last modified:December 1, 2001 - v1
Checksum:iACB066CF725F2C8F
GO

Sequence cautioni

The sequence AAF79535 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123K → R in AAK95305 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC023673 Genomic DNA Translation: AAF79535.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE32248.1
AF410319 mRNA Translation: AAK95305.1
AY050798 mRNA Translation: AAK92733.1
AY096360 mRNA Translation: AAM20001.1
PIRiC96521
RefSeqiNP_175244.1, NM_103706.4
UniGeneiAt.48286
At.70184

Genome annotation databases

EnsemblPlantsiAT1G48100.1; AT1G48100.1; AT1G48100
GeneIDi841228
GrameneiAT1G48100.1; AT1G48100.1; AT1G48100
KEGGiath:AT1G48100

Similar proteinsi

Entry informationi

Entry nameiPGLR4_ARATH
AccessioniPrimary (citable) accession number: Q949Z1
Secondary accession number(s): Q94EH4, Q9LNG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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