Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 6

Gene

UBP6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei113Nucleophile1
Active sitei430Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • calmodulin binding Source: TAIR
  • thiol-dependent ubiquitinyl hydrolase activity Source: UniProtKB-EC

GO - Biological processi

  • protein deubiquitination Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.094

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 6 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 6
Short name:
AtUBP6
Ubiquitin thioesterase 6
Ubiquitin-specific-processing protease 6
Gene namesi
Name:UBP6
Ordered Locus Names:At1g51710
ORF Names:F19C24.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G51710
TAIRilocus:2017552 AT1G51710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113C → S: Loss of activity. 1 Publication1
Mutagenesisi175W → R: Abolishes the calmodulin-binding. 1 Publication1
Mutagenesisi178L → K: Abolishes the calmodulin-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130331 – 482Ubiquitin carboxyl-terminal hydrolase 6Add BLAST482

Proteomic databases

PaxDbiQ949Y0
PRIDEiQ949Y0

PTM databases

iPTMnetiQ949Y0

Expressioni

Gene expression databases

ExpressionAtlasiQ949Y0 baseline and differential
GenevisibleiQ949Y0 AT

Interactioni

Subunit structurei

Interacts with calmodulin (CaM).1 Publication

GO - Molecular functioni

  • calmodulin binding Source: TAIR

Protein-protein interaction databases

BioGridi26821, 4 interactors
MINTiQ949Y0
STRINGi3702.AT1G51710.1

Structurei

3D structure databases

ProteinModelPortaliQ949Y0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 77Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76
Domaini104 – 478USPAdd BLAST375

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 191Calmodulin-bindingAdd BLAST20

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1872 Eukaryota
ENOG410XP96 LUCA
HOGENOMiHOG000202292
InParanoidiQ949Y0
KOiK11843
OMAiKVNQQPK
OrthoDBiEOG09360955
PhylomeDBiQ949Y0

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
PF00443 UCH, 1 hit
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q949Y0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTVSVKWQK KVLDGIEIDV SLPPYVFKAQ LYDLTGVPPE RQKIMVKGGL
60 70 80 90 100
LKDDGDWAAI GVKDGQKLMM MGTADEIVKA PEKAIVFAED LPEEALATNL
110 120 130 140 150
GYSAGLVNLG NTCYMNSTVQ CLKSVPELKS ALSNYSLAAR SNDVDQTSHM
160 170 180 190 200
LTVATRELFG ELDRSVNAVS PSQFWMVLRK KYPQFSQLQN GMHMQQDAEE
210 220 230 240 250
CWTQLLYTLS QSLKAPTSSE GADAVKALFG VNLQSRLHCQ ESGEESSETE
260 270 280 290 300
SVYSLKCHIS HEVNHLHEGL KHGLKGELEK TSPALGRTAL YVKESLIDSL
310 320 330 340 350
PRYLTVQFVR FFWKRESNQK AKILRKVDYP LVLDIFDLCS EDLRKKLEAP
360 370 380 390 400
RQKLREEEGK KLGLQTSAKS GSKDSDVKMT DAEASANGSG ESSTVNPQEG
410 420 430 440 450
TSSEKETHMT GIYDLVAVLT HKGRSADSGH YVAWVKQESG KWIQYDDDNP
460 470 480
SMQREEDITK LSGGGDWHMA YITMYKARFV SM
Length:482
Mass (Da):53,696
Last modified:December 1, 2001 - v1
Checksum:iECF357E9C5BEF3F7
GO

Sequence cautioni

The sequence AAG50872 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207Y → F in AAG42751 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302660 mRNA Translation: AAG42751.1
AC025294 Genomic DNA Translation: AAG50872.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE32705.1
AY050817 mRNA Translation: AAK92752.1
AY114081 mRNA Translation: AAM45129.1
BT000658 mRNA Translation: AAN31805.1
AY084730 mRNA Translation: AAM61304.1
AK221019 mRNA Translation: BAD94710.1
PIRiA96556
RefSeqiNP_564596.1, NM_104049.4 [Q949Y0-1]
UniGeneiAt.16941

Genome annotation databases

EnsemblPlantsiAT1G51710.1; AT1G51710.1; AT1G51710 [Q949Y0-1]
GeneIDi841596
GrameneiAT1G51710.1; AT1G51710.1; AT1G51710 [Q949Y0-1]
KEGGiath:AT1G51710

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP6_ARATH
AccessioniPrimary (citable) accession number: Q949Y0
Secondary accession number(s): Q56ZE7, Q9C8H9, Q9FPT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health