Q949Y0 (UBP6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 6
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 6
Short name=AtUBP6
Ubiquitin thioesterase 6
Ubiquitin-specific-processing protease 6

Gene names

Name:	UBP6
Ordered Locus Names:	At1g51710
ORF Names:	F19C24.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with calmodulin (CaM). Ref.7
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 ubiquitin-like domain.
Sequence caution	The sequence AAG50872.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	Calmodulin-binding
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Traceable author statement Ref.7. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	calmodulin binding Inferred from direct assay Ref.7. Source: TAIR ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from genetic interaction Ref.7. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q949Y0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Ubiquitin carboxyl-terminal hydrolase 6		PRO_0000313033
Regions
	Domain	2 – 77	76	Ubiquitin-like
	Region	172 – 191	20	Calmodulin-binding
Sites
	Active site	113	1	Nucleophile
	Active site	430	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	113	1	C → S: Loss of activity. Ref.7
	Mutagenesis	175	1	W → R: Abolishes the calmodulin-binding. Ref.7
	Mutagenesis	178	1	L → K: Abolishes the calmodulin-binding. Ref.7
	Sequence conflict	207	1	Y → F in AAG42751. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: ECF357E9C5BEF3F7	FASTA	482	53,696
	10 20 30 40 50 60 MPTVSVKWQK KVLDGIEIDV SLPPYVFKAQ LYDLTGVPPE RQKIMVKGGL LKDDGDWAAI 70 80 90 100 110 120 GVKDGQKLMM MGTADEIVKA PEKAIVFAED LPEEALATNL GYSAGLVNLG NTCYMNSTVQ 130 140 150 160 170 180 CLKSVPELKS ALSNYSLAAR SNDVDQTSHM LTVATRELFG ELDRSVNAVS PSQFWMVLRK 190 200 210 220 230 240 KYPQFSQLQN GMHMQQDAEE CWTQLLYTLS QSLKAPTSSE GADAVKALFG VNLQSRLHCQ 250 260 270 280 290 300 ESGEESSETE SVYSLKCHIS HEVNHLHEGL KHGLKGELEK TSPALGRTAL YVKESLIDSL 310 320 330 340 350 360 PRYLTVQFVR FFWKRESNQK AKILRKVDYP LVLDIFDLCS EDLRKKLEAP RQKLREEEGK 370 380 390 400 410 420 KLGLQTSAKS GSKDSDVKMT DAEASANGSG ESSTVNPQEG TSSEKETHMT GIYDLVAVLT 430 440 450 460 470 480 HKGRSADSGH YVAWVKQESG KWIQYDDDNP SMQREEDITK LSGGGDWHMA YITMYKARFV SM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 336-482. Strain: cv. Columbia.
[7]	"Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin." Moon B.C., Choi M.S., Kang Y.H., Kim M.C., Cheong M.S., Park C.Y., Yoo J.H., Koo S.C., Lee S.M., Lim C.O., Cho M.J., Chung W.S. FEBS Lett. 579:3885-3890(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CALMODULIN, MUTAGENESIS OF CYS-113; TRP-175 AND LEU-178.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302660 mRNA. Translation: AAG42751.1. AC025294 Genomic DNA. Translation: AAG50872.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE32705.1. AY050817 mRNA. Translation: AAK92752.1. AY114081 mRNA. Translation: AAM45129.1. BT000658 mRNA. Translation: AAN31805.1. AY084730 mRNA. Translation: AAM61304.1. AK221019 mRNA. Translation: BAD94710.1.
IPI	IPI00519355.
PIR	A96556.
RefSeq	NP_564596.1. NM_104049.3.
UniGene	At.16941.
3D structure databases
ProteinModelPortal	Q949Y0.
SMR	Q949Y0. Positions 4-478.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1351065.
Protein family/group databases
MEROPS	C19.094.
Proteomic databases
PaxDb	Q949Y0.
PRIDE	Q949Y0.
Protocols and materials databases
DNASU	841596.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G51710.1; AT1G51710.1; AT1G51710.
GeneID	841596.
KEGG	ath:AT1G51710.
Organism-specific databases
TAIR	At1g51710.
Phylogenomic databases
eggNOG	NOG286607.
HOGENOM	HOG000202292.
InParanoid	Q949Y0.
KO	K11843.
OMA	ELCTTEL.
PhylomeDB	Q949Y0.
ProtClustDB	CLSN2688687.
Gene expression databases
ArrayExpress	Q949Y0.
Genevestigator	Q949Y0.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP6_ARATH

Accession

Primary (citable) accession number: Q949Y0
Secondary accession number(s): Q56ZE7, Q9C8H9, Q9FPT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program