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Protein

Peroxiredoxin-2E, chloroplastic

Gene

PRXIIE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (By similarity). May be involved in chloroplast redox homeostasis (Probable).By similarity1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei121Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • defense response to bacterium Source: TAIR

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciARA:AT3G52960-MONOMER.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.

Protein family/group databases

PeroxiBasei4353. AtPrxII05.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-2E, chloroplastic (EC:1.11.1.15)
Alternative name(s):
Peroxiredoxin IIE
Thioredoxin peroxidase 2E
Gene namesi
Name:PRXIIE
Ordered Locus Names:At3g52960
ORF Names:F8J2_130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G52960.
TAIRilocus:2085181. AT3G52960.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastSequence analysisAdd BLAST70
ChainiPRO_000028228171 – 234Peroxiredoxin-2E, chloroplasticAdd BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ949U7.
PRIDEiQ949U7.

PTM databases

iPTMnetiQ949U7.
SwissPalmiQ949U7.

Expressioni

Tissue specificityi

Expressed in all tissues but predominantly in buds, siliques and seeds.2 Publications

Inductioni

Down-regulated by salt stress.1 Publication

Gene expression databases

ExpressionAtlasiQ949U7. baseline and differential.
GenevisibleiQ949U7. AT.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi9779. 3 interactors.
IntActiQ949U7. 1 interactor.
STRINGi3702.AT3G52960.1.

Structurei

3D structure databases

ProteinModelPortaliQ949U7.
SMRiQ949U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 234ThioredoxinPROSITE-ProRule annotationAdd BLAST162

Sequence similaritiesi

Belongs to the peroxiredoxin family. Prx5 subfamily.Curated

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0541. Eukaryota.
COG0678. LUCA.
HOGENOMiHOG000255884.
InParanoidiQ949U7.
OMAiGYINHPK.
OrthoDBiEOG09360OQV.
PhylomeDBiQ949U7.

Family and domain databases

InterProiView protein in InterPro
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF08534. Redoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q949U7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSLSVSRF MSSSATVISV AKPLLSPTVS FTAPLSFTRS LAPNLSLKFR
60 70 80 90 100
NRRTNSASAT TRSFATTPVT ASISVGDKLP DSTLSYLDPS TGDVKTVTVS
110 120 130 140 150
SLTAGKKTIL FAVPGAFTPT CSQKHVPGFV SKAGELRSKG IDVIACISVN
160 170 180 190 200
DAFVMEAWRK DLGINDEVML LSDGNGEFTG KLGVELDLRD KPVGLGVRSR
210 220 230
RYAILADDGV VKVLNLEEGG AFTNSSAEDM LKAL
Length:234
Mass (Da):24,684
Last modified:April 3, 2007 - v2
Checksum:i4F66DA63CD15F003
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133A → V in AAK92817 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132969 Genomic DNA. Translation: CAB86900.1.
CP002686 Genomic DNA. Translation: AEE79018.1.
AY050880 mRNA. Translation: AAK92817.1.
AY054638 mRNA. Translation: AAK96829.1.
AY072493 mRNA. Translation: AAL66908.1.
AY150397 mRNA. Translation: AAN12942.1.
PIRiPA0047.
T47553.
RefSeqiNP_190864.1. NM_115156.3.
UniGeneiAt.9858.

Genome annotation databases

EnsemblPlantsiAT3G52960.1; AT3G52960.1; AT3G52960.
GeneIDi824462.
GrameneiAT3G52960.1; AT3G52960.1; AT3G52960.
KEGGiath:AT3G52960.

Similar proteinsi

Entry informationi

Entry nameiPRX2E_ARATH
AccessioniPrimary (citable) accession number: Q949U7
Secondary accession number(s): Q9LF96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: September 27, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families