Reviewed, UniProtKB/Swiss-Prot Q949T8 (ASHR3_ARATH)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase ASHR3
    EC=2.1.1.43
Alternative name(s):
    ASH1-related protein 3
    Protein SET DOMAIN GROUP 4
    Protein stamen loss

Gene names

Name:	ASHR3
Synonyms:	SDG4, SET4, SML
Ordered Locus Names:	At4g30860
ORF Names:	F6I18.230

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Histone methyltransferase By similarity. Involved in stamen development.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subunit structure	Interacts with AMS/bHLH21 by its SET domain and PHD finger. Ref.5
Subcellular location	Nucleus. Note: Associated to the euchromatin. Ref.5
Tissue specificity	Expressed in roots, flowers and buds. the anther and in stamen filaments. Ref.5
Developmental stage	First observed at low levels in the vasculature and around hydathodes of developing leaves. In flowers, restricted to anthers tapetum at a post-meiosis stage, to filaments, and to microspores during their development. Disapears as pollen matures. In developing roots, expressed throughout the lower part, in the cap, in the epidermis and in non-epidermal tissue in the division and elongation zone. Also detected in cells lining lateral root formation.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 1 AWS domain. Contains 1 PHD-type zinc finger. Contains 1 post-SET domain. Contains 1 SET domain.
Sequence caution	The sequence CAA18207.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79804.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Chromatin regulator Developmental protein Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.5 Inferred from direct assay. Source: TAIR
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Histone-lysine N-methyltransferase ASHR3		PRO_0000233376
Regions
	Domain	283 – 326	44	AWS
	Domain	327 – 447	121	SET
	Domain	449 – 465	17	Post-SET
	Zinc finger	118 – 186	69	PHD-type
	Compositional bias	287 – 317	31	Cys-rich

Sequences

	Sequence		Length	Mass (Da)
	Q949T8-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 10D9C7EC779C2108	FASTA	497	56,122
	10 20 30 40 50 60 MLDLGNMSMS ASVALTCCPS FLPAASGPEL AKSINSPENL AGECNGKHLP MIPPEEEVKD 70 80 90 100 110 120 IKIANGVTAF TRKQNPSDRV KKGFVLDDHV KDWVKRRVAS GVSESTCFLP FLVGAKKMVD 130 140 150 160 170 180 CLVCHKPVYP GEDLSCSVRG CQGAYHSLCA KESLGFSKSS KFKCPQHECF VCKQRTQWRC 190 200 210 220 230 240 VKCPMAAHDK HSPWSKEILH LKDQPGRAVC WRHPTDWRLD TKHAVAQSEI EEVFCQLPLP 250 260 270 280 290 300 YVEEEFKIDL AWKDSVVKED PPSYVHIRRN IYLVKKKRDN ANDGVGCTNC GPNCDRSCVC 310 320 330 340 350 360 RVQCISCSKG CSCPESCGNR PFRKEKKIKI VKTEHCGWGV EAAESINKED FIVEYIGEVI 370 380 390 400 410 420 SDAQCEQRLW DMKHKGMKDF YMCEIQKDFT IDATFKGNAS RFLNHSCNPN CVLEKWQVEG 430 440 450 460 470 480 ETRVGVFAAR QIEAGEPLTY DYRFVQFGPE VKCNCGSENC QGYLGTKRKE PNCLVVSWGA 490 KRRRLFHRPI ARKPQQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The chromosome-associated protein SML regulates lateral stamen development in Arabidopsis." Cartagena J.A., Matsunaga S., Kurihara D., Fujimoto S., Azumi Y., Uchiyama S., Fukui K. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed: 11691919] [Abstract] Cited for: NOMENCLATURE.
[5]	"The Arabidopsis SET-domain protein ASHR3 is involved in stamen development and interacts with the bHLH transcription factor ABORTED MICROSPORES (AMS)." Thorstensen T., Grini P.E., Mercy I.S., Alm V., Erdal S., Aasland R., Aalen R.B. Plant Mol. Biol. 66:47-59(2008) [PubMed: 17978851] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH AMS.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB195469 Genomic DNA. Translation: BAD72877.1. AL022198 Genomic DNA. Translation: CAA18207.1. Sequence problems. AL161577 Genomic DNA. Translation: CAB79804.1. Sequence problems. AY050894 mRNA. Translation: AAK92831.1. AY096675 mRNA. Translation: AAM20309.1.
IPI	IPI00534865.
PIR	C85361.
RefSeq	NP_567859.1.
UniGene	At.26551
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q949T8.
Genome annotation databases
GeneID	829210.
GenomeReviews	Gene locus AT4G30860 in contig CT486007_GR.
KEGG	ath:AT4G30860.
NMPDR	fig|3702.1.peg.21075.
Organism-specific databases
TAIR	At4g30860.
Phylogenomic databases
OMA	YVHIRRN.
Enzyme and pathway databases
BRENDA	2.1.1.43. 302.
Gene expression databases
Genevestigator	Q949T8.
GermOnline	AT4G30860. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006560. AWS. IPR003616. Post-SET_Zn_bd. IPR001214. SET. IPR019786. Zinc_finger_PHD-type_CS. IPR001965. Znf_PHD. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00249. PHD. 1 hit. SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51215. AWS. 1 hit. PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASHR3_ARATH

Accession

Primary (citable) accession number: Q949T8
Secondary accession number(s): O65563

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)