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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic

Gene

GLY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids. Required for activation of systemic acquired resistance (SAR). Provision of glycerol-3-phosphate may be involved in generating lipid signals necessary for mediating defense responses and SAR.5 Publications

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.3 Publications

Kineticsi

  1. KM=0.33 µM for glycerone phosphate1 Publication
  1. Vmax=49 µmol/min/mg enzyme toward glycerone phosphate1 Publication

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171NADBy similarity1
Binding sitei194NAD; via amide nitrogenBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei228NAD; via amide nitrogenBy similarity1
Active sitei279Proton acceptorBy similarity1
Binding sitei343NADBy similarity1
Binding sitei369NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi94 – 99NADBy similarity6

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerolipid biosynthetic process Source: TAIR
  • glycerolipid metabolic process Source: TAIR
  • glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  • phospholipid biosynthetic process Source: UniProtKB-KW
  • systemic acquired resistance Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Plant defense
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00940

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic (EC:1.1.1.8)
Alternative name(s):
Protein SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY 1
Gene namesi
Name:GLY1
Synonyms:SFD1
Ordered Locus Names:At2g40690
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G40690
TAIRilocus:2064849 AT2G40690

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but leaves have decreased levels of hexadecatrienoic fatty acid (16:3) and increased levels of oleic acid (18:1).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi194K → A: Loss of activity. 1 Publication1
Mutagenesisi279K → A: Loss of activity. 1 Publication1
Mutagenesisi332D → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45ChloroplastSequence analysisAdd BLAST45
ChainiPRO_000042017446 – 420Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplasticAdd BLAST375

Proteomic databases

PaxDbiQ949Q0

Expressioni

Gene expression databases

ExpressionAtlasiQ949Q0 baseline and differential
GenevisibleiQ949Q0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40690.1

Structurei

3D structure databases

ProteinModelPortaliQ949Q0
SMRiQ949Q0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 344Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2711 Eukaryota
COG0240 LUCA
HOGENOMiHOG000246853
InParanoidiQ949Q0
KOiK00006
OMAiQPWFRCY
OrthoDBiEOG09360EQ1
PhylomeDBiQ949Q0

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
HAMAPiMF_00394 NAD_Glyc3P_dehydrog, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006168 G3P_DH_NAD-dep
IPR006109 G3P_DH_NAD-dep_C
IPR011128 G3P_DH_NAD-dep_N
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF07479 NAD_Gly3P_dh_C, 1 hit
PF01210 NAD_Gly3P_dh_N, 1 hit
PRINTSiPR00077 GPDHDRGNASE
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00957 NAD_G3PDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q949Q0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVQPACL DLHFSGKHPP LLKHNAIIVR CVSSPNVIPE ADSISGPPDI
60 70 80 90 100
INTNRDQRKV VRIAWEKLVR WSRSLRAKAK TDVLERTRKV VVLGGGSFGT
110 120 130 140 150
AMAAHVARRK EGLEVNMLVR DSFVCQSINE NHHNCKYFPE HKLPENVIAT
160 170 180 190 200
TDAKAALLDA DYCLHAVPVQ FSSSFLEGIA DYVDPGLPFI SLSKGLELNT
210 220 230 240 250
LRMMSQIIPI ALKNPRQPFV ALSGPSFALE LMNNLPTAMV VASKDKKLAN
260 270 280 290 300
AVQQLLASSY LRINTSSDVT GVEIAGALKN VLAIAAGIVD GMNLGNNSMA
310 320 330 340 350
ALVSQGCSEI RWLATKMGAK PTTITGLSGT GDIMLTCFVN LSRNRTVGVR
360 370 380 390 400
LGSGETLDDI LTSMNQVAEG VATAGAVIAL AQKYNVKLPV LTAVAKIIDN
410 420
ELTPTKAVLE LMNLPQIEEV
Length:420
Mass (Da):45,092
Last modified:December 1, 2001 - v1
Checksum:i3AACC248276BFC01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA Translation: AEC09864.1
CP002685 Genomic DNA Translation: ANM62576.1
AY050968 mRNA Translation: AAK93645.1
AY114078 mRNA Translation: AAM45126.1
RefSeqiNP_001324724.1, NM_001336858.1
NP_565939.1, NM_129631.3
UniGeneiAt.26537

Genome annotation databases

EnsemblPlantsiAT2G40690.1; AT2G40690.1; AT2G40690
AT2G40690.3; AT2G40690.3; AT2G40690
GeneIDi818664
GrameneiAT2G40690.1; AT2G40690.1; AT2G40690
AT2G40690.3; AT2G40690.3; AT2G40690
KEGGiath:AT2G40690

Similar proteinsi

Entry informationi

Entry nameiGPDA2_ARATH
AccessioniPrimary (citable) accession number: Q949Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health