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Q949Q0

- GPDA2_ARATH

UniProt

Q949Q0 - GPDA2_ARATH

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Protein
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic
Gene
GLY1, SFD1, At2g40690
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids. Required for activation of systemic acquired resistance (SAR). Provision of glycerol-3-phosphate may be involved in generating lipid signals necessary for mediating defense responses and SAR.5 Publications

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.3 Publications

Kineticsi

  1. KM=0.33 µM for glycerone phosphate1 Publication

Vmax=49 µmol/min/mg enzyme toward glycerone phosphate

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711NAD By similarity
Binding sitei194 – 1941NAD; via amide nitrogen By similarity
Binding sitei194 – 1941Substrate By similarity
Binding sitei228 – 2281NAD; via amide nitrogen By similarity
Active sitei279 – 2791Proton acceptor By similarity
Binding sitei343 – 3431NAD By similarity
Binding sitei369 – 3691NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi94 – 996NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate catabolic process Source: InterPro
  3. glycerolipid biosynthetic process Source: TAIR
  4. glycerolipid metabolic process Source: TAIR
  5. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  6. phospholipid biosynthetic process Source: UniProtKB-KW
  7. systemic acquired resistance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Plant defense

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic (EC:1.1.1.8)
Alternative name(s):
Protein SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY 1
Gene namesi
Name:GLY1
Synonyms:SFD1
Ordered Locus Names:At2g40690
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G40690.

Subcellular locationi

Plastidchloroplast By similarity UniRule annotation

GO - Cellular componenti

  1. chloroplast envelope Source: TAIR
  2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but leaves have decreased levels of hexadecatrienoic fatty acid (16:3) and increased levels of oleic acid (18:1).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi194 – 1941K → A: Loss of activity. 1 Publication
Mutagenesisi279 – 2791K → A: Loss of activity. 1 Publication
Mutagenesisi332 – 3321D → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545Chloroplast Reviewed prediction
Add
BLAST
Chaini46 – 420375Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplasticUniRule annotation
PRO_0000420174Add
BLAST

Proteomic databases

PaxDbiQ949Q0.
PRIDEiQ949Q0.

Expressioni

Gene expression databases

GenevestigatoriQ949Q0.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40690.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ949Q0.
SMRiQ949Q0. Positions 89-412.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 3442Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0240.
HOGENOMiHOG000246853.
InParanoidiQ949Q0.
OMAiMPISTAV.
PhylomeDBiQ949Q0.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q949Q0-1 [UniParc]FASTAAdd to Basket

« Hide

MAASVQPACL DLHFSGKHPP LLKHNAIIVR CVSSPNVIPE ADSISGPPDI    50
INTNRDQRKV VRIAWEKLVR WSRSLRAKAK TDVLERTRKV VVLGGGSFGT 100
AMAAHVARRK EGLEVNMLVR DSFVCQSINE NHHNCKYFPE HKLPENVIAT 150
TDAKAALLDA DYCLHAVPVQ FSSSFLEGIA DYVDPGLPFI SLSKGLELNT 200
LRMMSQIIPI ALKNPRQPFV ALSGPSFALE LMNNLPTAMV VASKDKKLAN 250
AVQQLLASSY LRINTSSDVT GVEIAGALKN VLAIAAGIVD GMNLGNNSMA 300
ALVSQGCSEI RWLATKMGAK PTTITGLSGT GDIMLTCFVN LSRNRTVGVR 350
LGSGETLDDI LTSMNQVAEG VATAGAVIAL AQKYNVKLPV LTAVAKIIDN 400
ELTPTKAVLE LMNLPQIEEV 420
Length:420
Mass (Da):45,092
Last modified:December 1, 2001 - v1
Checksum:i3AACC248276BFC01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002685 Genomic DNA. Translation: AEC09864.1.
AY050968 mRNA. Translation: AAK93645.1.
AY114078 mRNA. Translation: AAM45126.1.
RefSeqiNP_565939.1. NM_129631.2.
UniGeneiAt.26537.

Genome annotation databases

EnsemblPlantsiAT2G40690.1; AT2G40690.1; AT2G40690.
GeneIDi818664.
KEGGiath:AT2G40690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002685 Genomic DNA. Translation: AEC09864.1 .
AY050968 mRNA. Translation: AAK93645.1 .
AY114078 mRNA. Translation: AAM45126.1 .
RefSeqi NP_565939.1. NM_129631.2.
UniGenei At.26537.

3D structure databases

ProteinModelPortali Q949Q0.
SMRi Q949Q0. Positions 89-412.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G40690.1-P.

Proteomic databases

PaxDbi Q949Q0.
PRIDEi Q949Q0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G40690.1 ; AT2G40690.1 ; AT2G40690 .
GeneIDi 818664.
KEGGi ath:AT2G40690.

Organism-specific databases

TAIRi AT2G40690.

Phylogenomic databases

eggNOGi COG0240.
HOGENOMi HOG000246853.
InParanoidi Q949Q0.
OMAi MPISTAV.
PhylomeDBi Q949Q0.

Enzyme and pathway databases

UniPathwayi UPA00940 .

Miscellaneous databases

PROi Q949Q0.

Gene expression databases

Genevestigatori Q949Q0.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_00394. NAD_Glyc3P_dehydrog.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
PROSITEi PS00957. NAD_G3PDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The Arabidopsis thaliana dihydroxyacetone phosphate reductase gene SUPPRESSSOR OF FATTY ACID DESATURASE DEFICIENCY1 is required for glycerolipid metabolism and for the activation of systemic acquired resistance."
    Nandi A., Welti R., Shah J.
    Plant Cell 16:465-477(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  5. "Oleic acid levels regulated by glycerolipid metabolism modulate defense gene expression in Arabidopsis."
    Kachroo A., Venugopal S.C., Lapchyk L., Falcone D., Hildebrand D., Kachroo P.
    Proc. Natl. Acad. Sci. U.S.A. 101:5152-5157(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  6. "Plastidial fatty acid levels regulate resistance gene-dependent defense signaling in Arabidopsis."
    Chandra-Shekara A.C., Venugopal S.C., Barman S.R., Kachroo A., Kachroo P.
    Proc. Natl. Acad. Sci. U.S.A. 104:7277-7282(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Plastid omega3-fatty acid desaturase-dependent accumulation of a systemic acquired resistance inducing activity in petiole exudates of Arabidopsis thaliana is independent of jasmonic acid."
    Chaturvedi R., Krothapalli K., Makandar R., Nandi A., Sparks A.A., Roth M.R., Welti R., Shah J.
    Plant J. 54:106-117(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Biochemical and molecular-genetic characterization of SFD1's involvement in lipid metabolism and defense signaling."
    Lorenc-Kukula K., Chaturvedi R., Roth M., Welti R., Shah J.
    Front. Plant Sci. 3:26-26(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF LYS-194; LYS-279 AND ASP-332.

Entry informationi

Entry nameiGPDA2_ARATH
AccessioniPrimary (citable) accession number: Q949Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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