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Q949Q0

- GPDA2_ARATH

UniProt

Q949Q0 - GPDA2_ARATH

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic

Gene

GLY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids. Required for activation of systemic acquired resistance (SAR). Provision of glycerol-3-phosphate may be involved in generating lipid signals necessary for mediating defense responses and SAR.5 Publications

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.3 Publications

    Kineticsi

    1. KM=0.33 µM for glycerone phosphate1 Publication

    Vmax=49 µmol/min/mg enzyme toward glycerone phosphate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei171 – 1711NADBy similarity
    Binding sitei194 – 1941NAD; via amide nitrogenBy similarity
    Binding sitei194 – 1941SubstrateBy similarity
    Binding sitei228 – 2281NAD; via amide nitrogenBy similarity
    Active sitei279 – 2791Proton acceptorBy similarity
    Binding sitei343 – 3431NADBy similarity
    Binding sitei369 – 3691NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi94 – 996NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: TAIR
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycerol-3-phosphate catabolic process Source: InterPro
    3. glycerolipid biosynthetic process Source: TAIR
    4. glycerolipid metabolic process Source: TAIR
    5. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
    6. phospholipid biosynthetic process Source: UniProtKB-KW
    7. systemic acquired resistance Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Plant defense

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00940.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic (EC:1.1.1.8)
    Alternative name(s):
    Protein SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY 1
    Gene namesi
    Name:GLY1
    Synonyms:SFD1
    Ordered Locus Names:At2g40690
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G40690.

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast envelope Source: TAIR
    2. glycerol-3-phosphate dehydrogenase complex Source: InterPro

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but leaves have decreased levels of hexadecatrienoic fatty acid (16:3) and increased levels of oleic acid (18:1).2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi194 – 1941K → A: Loss of activity. 1 Publication
    Mutagenesisi279 – 2791K → A: Loss of activity. 1 Publication
    Mutagenesisi332 – 3321D → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545ChloroplastSequence AnalysisAdd
    BLAST
    Chaini46 – 420375Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplasticPRO_0000420174Add
    BLAST

    Proteomic databases

    PaxDbiQ949Q0.
    PRIDEiQ949Q0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ949Q0.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G40690.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ949Q0.
    SMRiQ949Q0. Positions 89-412.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni343 – 3442Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0240.
    HOGENOMiHOG000246853.
    InParanoidiQ949Q0.
    OMAiMPISTAV.
    PhylomeDBiQ949Q0.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_00394. NAD_Glyc3P_dehydrog.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q949Q0-1 [UniParc]FASTAAdd to Basket

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    MAASVQPACL DLHFSGKHPP LLKHNAIIVR CVSSPNVIPE ADSISGPPDI    50
    INTNRDQRKV VRIAWEKLVR WSRSLRAKAK TDVLERTRKV VVLGGGSFGT 100
    AMAAHVARRK EGLEVNMLVR DSFVCQSINE NHHNCKYFPE HKLPENVIAT 150
    TDAKAALLDA DYCLHAVPVQ FSSSFLEGIA DYVDPGLPFI SLSKGLELNT 200
    LRMMSQIIPI ALKNPRQPFV ALSGPSFALE LMNNLPTAMV VASKDKKLAN 250
    AVQQLLASSY LRINTSSDVT GVEIAGALKN VLAIAAGIVD GMNLGNNSMA 300
    ALVSQGCSEI RWLATKMGAK PTTITGLSGT GDIMLTCFVN LSRNRTVGVR 350
    LGSGETLDDI LTSMNQVAEG VATAGAVIAL AQKYNVKLPV LTAVAKIIDN 400
    ELTPTKAVLE LMNLPQIEEV 420
    Length:420
    Mass (Da):45,092
    Last modified:December 1, 2001 - v1
    Checksum:i3AACC248276BFC01
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002685 Genomic DNA. Translation: AEC09864.1.
    AY050968 mRNA. Translation: AAK93645.1.
    AY114078 mRNA. Translation: AAM45126.1.
    RefSeqiNP_565939.1. NM_129631.2.
    UniGeneiAt.26537.

    Genome annotation databases

    EnsemblPlantsiAT2G40690.1; AT2G40690.1; AT2G40690.
    GeneIDi818664.
    KEGGiath:AT2G40690.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002685 Genomic DNA. Translation: AEC09864.1 .
    AY050968 mRNA. Translation: AAK93645.1 .
    AY114078 mRNA. Translation: AAM45126.1 .
    RefSeqi NP_565939.1. NM_129631.2.
    UniGenei At.26537.

    3D structure databases

    ProteinModelPortali Q949Q0.
    SMRi Q949Q0. Positions 89-412.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G40690.1-P.

    Proteomic databases

    PaxDbi Q949Q0.
    PRIDEi Q949Q0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G40690.1 ; AT2G40690.1 ; AT2G40690 .
    GeneIDi 818664.
    KEGGi ath:AT2G40690.

    Organism-specific databases

    TAIRi AT2G40690.

    Phylogenomic databases

    eggNOGi COG0240.
    HOGENOMi HOG000246853.
    InParanoidi Q949Q0.
    OMAi MPISTAV.
    PhylomeDBi Q949Q0.

    Enzyme and pathway databases

    UniPathwayi UPA00940 .

    Miscellaneous databases

    PROi Q949Q0.

    Gene expression databases

    Genevestigatori Q949Q0.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_00394. NAD_Glyc3P_dehydrog.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "The Arabidopsis thaliana dihydroxyacetone phosphate reductase gene SUPPRESSSOR OF FATTY ACID DESATURASE DEFICIENCY1 is required for glycerolipid metabolism and for the activation of systemic acquired resistance."
      Nandi A., Welti R., Shah J.
      Plant Cell 16:465-477(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    5. "Oleic acid levels regulated by glycerolipid metabolism modulate defense gene expression in Arabidopsis."
      Kachroo A., Venugopal S.C., Lapchyk L., Falcone D., Hildebrand D., Kachroo P.
      Proc. Natl. Acad. Sci. U.S.A. 101:5152-5157(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    6. "Plastidial fatty acid levels regulate resistance gene-dependent defense signaling in Arabidopsis."
      Chandra-Shekara A.C., Venugopal S.C., Barman S.R., Kachroo A., Kachroo P.
      Proc. Natl. Acad. Sci. U.S.A. 104:7277-7282(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Plastid omega3-fatty acid desaturase-dependent accumulation of a systemic acquired resistance inducing activity in petiole exudates of Arabidopsis thaliana is independent of jasmonic acid."
      Chaturvedi R., Krothapalli K., Makandar R., Nandi A., Sparks A.A., Roth M.R., Welti R., Shah J.
      Plant J. 54:106-117(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Biochemical and molecular-genetic characterization of SFD1's involvement in lipid metabolism and defense signaling."
      Lorenc-Kukula K., Chaturvedi R., Roth M., Welti R., Shah J.
      Front. Plant Sci. 3:26-26(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF LYS-194; LYS-279 AND ASP-332.

    Entry informationi

    Entry nameiGPDA2_ARATH
    AccessioniPrimary (citable) accession number: Q949Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3