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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic

Gene

GLY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids. Required for activation of systemic acquired resistance (SAR). Provision of glycerol-3-phosphate may be involved in generating lipid signals necessary for mediating defense responses and SAR.5 Publications

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.3 Publications

Kineticsi

  1. KM=0.33 µM for glycerone phosphate1 Publication
  1. Vmax=49 µmol/min/mg enzyme toward glycerone phosphate1 Publication

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171NADBy similarity1
Binding sitei194NAD; via amide nitrogenBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei228NAD; via amide nitrogenBy similarity1
Active sitei279Proton acceptorBy similarity1
Binding sitei343NADBy similarity1
Binding sitei369NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi94 – 99NADBy similarity6

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerolipid biosynthetic process Source: TAIR
  • glycerolipid metabolic process Source: TAIR
  • glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  • phospholipid biosynthetic process Source: UniProtKB-KW
  • systemic acquired resistance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Plant defense

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic (EC:1.1.1.8)
Alternative name(s):
Protein SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY 1
Gene namesi
Name:GLY1
Synonyms:SFD1
Ordered Locus Names:At2g40690
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40690.

Subcellular locationi

GO - Cellular componenti

  • chloroplast envelope Source: TAIR
  • glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but leaves have decreased levels of hexadecatrienoic fatty acid (16:3) and increased levels of oleic acid (18:1).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi194K → A: Loss of activity. 1 Publication1
Mutagenesisi279K → A: Loss of activity. 1 Publication1
Mutagenesisi332D → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45ChloroplastSequence analysisAdd BLAST45
ChainiPRO_000042017446 – 420Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplasticAdd BLAST375

Proteomic databases

PaxDbiQ949Q0.

Expressioni

Gene expression databases

GenevisibleiQ949Q0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40690.1.

Structurei

3D structure databases

ProteinModelPortaliQ949Q0.
SMRiQ949Q0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni343 – 344Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
HOGENOMiHOG000246853.
InParanoidiQ949Q0.
KOiK00006.
OMAiDYLPGVE.
OrthoDBiEOG09360EQ1.
PhylomeDBiQ949Q0.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q949Q0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVQPACL DLHFSGKHPP LLKHNAIIVR CVSSPNVIPE ADSISGPPDI
60 70 80 90 100
INTNRDQRKV VRIAWEKLVR WSRSLRAKAK TDVLERTRKV VVLGGGSFGT
110 120 130 140 150
AMAAHVARRK EGLEVNMLVR DSFVCQSINE NHHNCKYFPE HKLPENVIAT
160 170 180 190 200
TDAKAALLDA DYCLHAVPVQ FSSSFLEGIA DYVDPGLPFI SLSKGLELNT
210 220 230 240 250
LRMMSQIIPI ALKNPRQPFV ALSGPSFALE LMNNLPTAMV VASKDKKLAN
260 270 280 290 300
AVQQLLASSY LRINTSSDVT GVEIAGALKN VLAIAAGIVD GMNLGNNSMA
310 320 330 340 350
ALVSQGCSEI RWLATKMGAK PTTITGLSGT GDIMLTCFVN LSRNRTVGVR
360 370 380 390 400
LGSGETLDDI LTSMNQVAEG VATAGAVIAL AQKYNVKLPV LTAVAKIIDN
410 420
ELTPTKAVLE LMNLPQIEEV
Length:420
Mass (Da):45,092
Last modified:December 1, 2001 - v1
Checksum:i3AACC248276BFC01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA. Translation: AEC09864.1.
AY050968 mRNA. Translation: AAK93645.1.
AY114078 mRNA. Translation: AAM45126.1.
RefSeqiNP_565939.1. NM_129631.3.
UniGeneiAt.26537.

Genome annotation databases

EnsemblPlantsiAT2G40690.1; AT2G40690.1; AT2G40690.
GeneIDi818664.
GrameneiAT2G40690.1; AT2G40690.1; AT2G40690.
KEGGiath:AT2G40690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA. Translation: AEC09864.1.
AY050968 mRNA. Translation: AAK93645.1.
AY114078 mRNA. Translation: AAM45126.1.
RefSeqiNP_565939.1. NM_129631.3.
UniGeneiAt.26537.

3D structure databases

ProteinModelPortaliQ949Q0.
SMRiQ949Q0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G40690.1.

Proteomic databases

PaxDbiQ949Q0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40690.1; AT2G40690.1; AT2G40690.
GeneIDi818664.
GrameneiAT2G40690.1; AT2G40690.1; AT2G40690.
KEGGiath:AT2G40690.

Organism-specific databases

TAIRiAT2G40690.

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
HOGENOMiHOG000246853.
InParanoidiQ949Q0.
KOiK00006.
OMAiDYLPGVE.
OrthoDBiEOG09360EQ1.
PhylomeDBiQ949Q0.

Enzyme and pathway databases

UniPathwayiUPA00940.

Miscellaneous databases

PROiQ949Q0.

Gene expression databases

GenevisibleiQ949Q0. AT.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPDA2_ARATH
AccessioniPrimary (citable) accession number: Q949Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.