ID   ABAH1_ARATH             Reviewed;         467 AA.
AC   Q949P1; O65624;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=Abscisic acid 8'-hydroxylase 1;
DE            Short=ABA 8'-hydroxylase 1;
DE            EC=1.14.13.93;
DE   AltName: Full=Cytochrome P450 707A1;
GN   Name=CYP707A1; OrderedLocusNames=At4g19230; ORFNames=T18B16.200;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   INDUCTION.
RX   PubMed=15064374; DOI=10.1104/pp.103.037614;
RA   Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K.,
RA   Mizutani M.;
RT   "Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key
RT   enzyme in the oxidative catabolism of abscisic acid.";
RL   Plant Physiol. 134:1439-1449(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   INDUCTION.
RX   PubMed=15044947; DOI=10.1038/sj.emboj.7600121;
RA   Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S.,
RA   Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.;
RT   "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases:
RT   key enzymes in ABA catabolism.";
RL   EMBO J. 23:1647-1656(2004).
RN   [5]
RP   FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=16543410; DOI=10.1104/pp.106.079475;
RA   Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N.,
RA   Kamiya Y., Koshiba T., Nambara E.;
RT   "CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases,
RT   are indispensable for proper control of seed dormancy and germination
RT   in Arabidopsis.";
RL   Plant Physiol. 141:97-107(2006).
RN   [6]
RP   INDUCTION BY PHYTOCHROME B.
RX   PubMed=17010113; DOI=10.1111/j.1365-313X.2006.02881.x;
RA   Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y.,
RA   North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y.,
RA   Yamaguchi S., Nambara E.;
RT   "Regulation of hormone metabolism in Arabidopsis seeds: phytochrome
RT   regulation of abscisic acid metabolism and abscisic acid regulation of
RT   gibberellin metabolism.";
RL   Plant J. 48:354-366(2006).
CC   -!- FUNCTION: Involved in the oxidative degradation of abscisic acid.
CC       Plays an important role in determining abscisic acid levels in dry
CC       seeds and in the control of postgermination growth.
CC   -!- CATALYTIC ACTIVITY: (+)-abscisate + NADPH + O(2) = 8'-
CC       hydroxyabscisate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Plant hormone degradation; abscisic acid degradation.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences;
CC       Name=1;
CC         IsoId=Q949P1-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Mainly expressed in flowers, siliques, roots
CC       and stems. Lower expression in rosette leaves and dry seeds.
CC       Expressed in vascular tissues of embryo during the seed
CC       development.
CC   -!- DEVELOPMENTAL STAGE: Expressed predominantly during mid-maturation
CC       of seed and down-regulated during late maturation. Up-regulated 12
CC       hours after seed imbibition.
CC   -!- INDUCTION: By abscisic acid treatment, salt or osmotic stresses,
CC       and by dehydration and rehydration. Expression regulated by
CC       phytochrome B.
CC   -!- DISRUPTION PHENOTYPE: Plants show a reduced germination.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA16713.1; Type=Erroneous gene model prediction;
CC       Sequence=CAB78925.1; Type=Erroneous gene model prediction;
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DR   EMBL; AB122149; BAD16629.1; -; mRNA.
DR   EMBL; AL021687; CAA16713.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161550; CAB78925.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY050980; AAK93657.1; -; mRNA.
DR   EMBL; AY091446; AAM14385.1; -; mRNA.
DR   IPI; IPI00519786; -.
DR   PIR; T04444; T04444.
DR   RefSeq; NP_567581.1; -.
DR   UniGene; At.1930; -.
DR   HSSP; P33006; 1CPT.
DR   GeneID; 827663; -.
DR   GenomeReviews; CT486007_GR; AT4G19230.
DR   KEGG; ath:AT4G19230; -.
DR   NMPDR; fig|3702.1.peg.19741; -.
DR   GeneFarm; 1251; 94.
DR   TAIR; At4g19230; -.
DR   BioCyc; MetaCyc:AT4G19230-MON; -.
DR   BRENDA; 1.14.13.93; 302.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010295; F:(+)-abscisic acid 8'-hydroxylase activity; IDA:TAIR.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0009687; P:abscisic acid metabolic process; IMP:TAIR.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0048838; P:release of seed from dormancy; IMP:TAIR.
DR   GO; GO:0009639; P:response to red or far red light; IEP:TAIR.
DR   GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Heme; Iron; Membrane;
KW   Metal-binding; Monooxygenase; NADP; Oxidoreductase; Stress response;
KW   Transmembrane.
FT   CHAIN         1    467       Abscisic acid 8'-hydroxylase 1.
FT                                /FTId=PRO_0000288639.
FT   TRANSMEM      5     24       Potential.
FT   METAL       411    411       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   467 AA;  53037 MW;  2F4230446536D955 CRC64;
     MDISALFLTL FAGSLFLYFL RCLISQRRFG SSKLPLPPGT MGWPYVGETF QLYSQDPNVF
     FQSKQKRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL FKPTFPASKE RMLGKQAIFF
     HQGDYHAKLR KLVLRAFMPE SIRNMVPDIE SIAQDSLRSW EGTMINTYQE MKTYTFNVAL
     LSIFGKDEVL YREDLKRCYY ILEKGYNSMP VNLPGTLFHK SMKARKELSQ ILARILSERR
     QNGSSHNDLL GSFMGDKEEL TDEQIADNII GVIFAARDTT ASVMSWILKY LAENPNVLEA
     VTEEQMAIRK DKEEGESLTW GDTKKMPLTS RVIQETLRVA SILSFTFREA VEDVEYEGYL
     IPKGWKVLPL FRNIHHSADI FSNPGKFDPS RFEVAPKPNT FMPFGNGTHS CPGNELAKLE
     MSIMIHHLTT KYSWSIVGAS DGIQYGPFAL PQNGLPIVLA RKPEIEV
//