Q949P1 (ABAH1_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 79.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Abscisic acid 8'-hydroxylase 1 Short name=ABA 8'-hydroxylase 1 EC=1.14.13.93 Alternative name(s): Cytochrome P450 707A1 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 467 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Involved in the oxidative degradation of abscisic acid. Plays an important role in determining abscisic acid levels in dry seeds and in the control of postgermination growth. Ref.1 Ref.5 Ref.6 |
| Catalytic activity | +-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O. |
| Cofactor | Heme group By similarity. |
| Pathway | |
| Subcellular location | Membrane; Single-pass membrane protein Potential. |
| Tissue specificity | Mainly expressed in flowers, siliques, roots and stems. Lower expression in rosette leaves and dry seeds. Expressed in vascular tissues of embryo during the seed development. Ref.1 Ref.5 Ref.6 |
| Developmental stage | Expressed predominantly during mid-maturation of seed and down-regulated during late maturation. Up-regulated 12 hours after seed imbibition. Ref.5 Ref.6 |
| Induction | By abscisic acid treatment, salt or osmotic stresses, and by dehydration and rehydration. Expression regulated by phytochrome B. Ref.1 Ref.5 Ref.7 |
| Disruption phenotype | Plants show a reduced germination. Ref.6 |
| Sequence similarities | Belongs to the cytochrome P450 family. |
| Sequence caution | The sequence CAA16713.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB78925.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Stress response |
| Cellular component | Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | abscisic acid metabolic process Inferred from mutant phenotype Ref.6. Source: TAIR defense response to fungusInferred from expression pattern. Source: TAIR release of seed from dormancyInferred from mutant phenotype. Source: TAIR response to red or far red lightInferred from expression pattern Ref.7. Source: TAIR |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | (+)-abscisic acid 8'-hydroxylase activity Inferred from direct assay Ref.1. Source: TAIR electron carrier activityInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: Q949P1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid." Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., Mizutani M. Plant Physiol. 134:1439-1449(2004) [PubMed: 15064374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION. |
| [2] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism." Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E. EMBO J. 23:1647-1656(2004) [PubMed: 15044947] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. |
| [6] | "CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis." Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N., Kamiya Y., Koshiba T., Nambara E. Plant Physiol. 141:97-107(2006) [PubMed: 16543410] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. |
| [7] | "Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism." Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E. Plant J. 48:354-366(2006) [PubMed: 17010113] [Abstract] Cited for: INDUCTION BY PHYTOCHROME B. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB122149 mRNA. Translation: BAD16629.1. AL021687 Genomic DNA. Translation: CAA16713.1. Sequence problems. AL161550 Genomic DNA. Translation: CAB78925.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE84162.1. AY050980 mRNA. Translation: AAK93657.1. AY091446 mRNA. Translation: AAM14385.1. |
| IPI | IPI00519786. |
| PIR | T04444. |
| RefSeq | NP_567581.1. NM_118043.1. |
| UniGene | At.1930. |
3D structure databases | |
| ProteinModelPortal | Q949P1. |
| SMR | Q949P1. Positions 33-461. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q949P1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G19230.1; AT4G19230.1; AT4G19230. |
| GeneID | 827663. |
| GenomeReviews | Gene locus AT4G19230 in contig CT486007_GR. |
| KEGG | ath:AT4G19230. |
| NMPDR | fig|3702.1.peg.19741. |
Organism-specific databases | |
| GeneFarm | 1251. 94. |
| TAIR | At4g19230. |
Phylogenomic databases | |
| GeneTree | EPGT00070000028041. |
| HOGENOM | HBG744016. |
| InParanoid | Q949P1. |
| PhylomeDB | Q949P1. |
| ProtClustDB | PLN02196. |
Enzyme and pathway databases | |
| BioCyc | ARA:AT4G19230-MONOMER. MetaCyc:AT4G19230-MONOMER. |
Gene expression databases | |
| Genevestigator | Q949P1. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| KO | K09843. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| SUPFAM | SSF48264. Cytochrome_P450. 1 hit. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ABAH1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q949P1 Secondary accession number(s): O65624 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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