ID   FABG3_BRANA             Reviewed;         315 AA.
AC   Q949M3;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 100.
DE   RecName: Full=3-oxoacyl-[acyl-carrier-protein] reductase 3, chloroplastic;
DE            EC=1.1.1.100;
DE   AltName: Full=3-ketoacyl-acyl carrier protein reductase 3;
DE   Flags: Precursor;
GN   Name=bkr3;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Jet neuf; TISSUE=Embryo;
RA   McDonald F.S., White A.J., Elborough K.M., Slabas A.R.;
RL   Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+)
CC         + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA-
CC         COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100;
CC   -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}. Plastid
CC       {ECO:0000250}. Note=And non-photosynthetic plastids. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; AJ243085; CAC41364.1; -; mRNA.
DR   RefSeq; NP_001303056.1; NM_001316127.1.
DR   AlphaFoldDB; Q949M3; -.
DR   SMR; Q949M3; -.
DR   EnsemblPlants; CDX84941; CDX84941; GSBRNA2T00142300001.
DR   GeneID; 106401718; -.
DR   Gramene; CDX84941; CDX84941; GSBRNA2T00142300001.
DR   KEGG; bna:106401718; -.
DR   OMA; LFGVQCD; -.
DR   OrthoDB; 2263846at2759; -.
DR   BRENDA; 1.1.1.100; 944.
DR   UniPathway; UPA00094; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004316; F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05333; BKR_SDR_c; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR011284; 3oxo_ACP_reduc.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   NCBIfam; TIGR01830; 3oxo_ACP_reduc; 1.
DR   PANTHER; PTHR42879; 3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE; 1.
DR   PANTHER; PTHR42879:SF2; 3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SMART; SM00822; PKS_KR; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT         1..55
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           56..315
FT                   /note="3-oxoacyl-[acyl-carrier-protein] reductase 3,
FT                   chloroplastic"
FT                   /id="PRO_0000031979"
FT   ACT_SITE        222
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         77..101
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         209
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   315 AA;  33140 MW;  3B0B36C84A4FB313 CRC64;
     MATTVAATKL TSLKAVKKLG FREIRQVRQW TPLQSSMPHF GSRQSFATST VVKAQATAVE
     QSTGEAVPKV ESPVVVVTGA SRGIGKAIAL SLGKAGCKVL VNYARSAKEA EEVSKQIEAY
     GGQAITFGGD VSKEADVEAM MKTAIDAWGT IDVVVNNAGI TRDTLLIRMK KSQWDEVIDL
     NLTGVFLCTQ AATKIMMKKR KGRIINIASV VGLIGNIGQA NYAAAKAGVI GFSKTAAREG
     ASRNINVNVV CPGFIASDMT AKLGEDMEKK ILGTIPLGRY GQPEDVAGLV EFLALSPAAS
     YITGQAFTID GGIAI
//