Q949J1 (IPYR2_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble inorganic pyrophosphatase 2
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase 2
Short name=PPase 2

Gene names

Name:	ppa2
Synonyms:	ppaII

Organism

Chlamydomonas reinhardtii (Chlamydomonas smithii)

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. Ref.1
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. UniProtKB P0A7A9
Subunit structure	Monomer. Ref.1
Subcellular location	Mitochondrion Ref.1.
Post-translational modification	The N-terminus is blocked. Ref.1
Sequence similarities	Belongs to the PPase family.
Biophysicochemical properties	Kinetic parameters: KM=65.2 µM for Mg2-PPi Ref.1 pH dependence: Optimum pH is 7.5. Ref.1
Mass spectrometry	Molecular mass is 24350 Da from positions 1 - 192. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	inorganic diphosphatase activity Inferred from direct assay Ref.1. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Soluble inorganic pyrophosphatase 2		PRO_0000253939
Sites
	Metal binding	74	1	Magnesium 1 By similarity UniProtKB P0A7A9
	Metal binding	79	1	Magnesium 1 By similarity UniProtKB P0A7A9
	Metal binding	79	1	Magnesium 2 By similarity UniProtKB P0A7A9
	Metal binding	111	1	Magnesium 1 By similarity UniProtKB P0A7A9

Sequences

	Sequence		Length	Mass (Da)
	Q949J1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A1CA190C3C3FE597	FASTA	192	22,175
	10 20 30 40 50 60 MSFYRGTASH PWHDLHPGND APNFVSCVIE IPRGSKVKYE LDKDTGLCFV DRILYSSVVY 70 80 90 100 110 120 PHNYGFVPKT LCEDGDPLDV LVLMQEPVVP MCFLRAKPIG VMQMLDQGER DDKLIAVHAD 130 140 150 160 170 180 DPEYKGFTDI SQLPPHRLAE IKRFFEDYKK NEHKEVVVDD FLGAEEAKKV VKDSLNMYQE 190 HYVPRKLRNV YE

References

[1]	"A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes." Gomez-Garcia M.R., Losada M., Serrano A. Biochem. J. 395:211-221(2006) [PubMed: 16313235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Strain: 21gr / CC-1690.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ298232 mRNA. Translation: CAC42763.1.
RefSeq	XP_001694912.1. XM_001694860.1.
UniGene	Cre.13286.
3D structure databases
HSSP	HSSP built from PDB template 1TWL based on UniProtKB Q8U438.
ProteinModelPortal	Q949J1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q949J1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	EDP02064; EDP02064; CHLREDRAFT_174103.
GeneID	5720576.
KEGG	cre:CHLREDRAFT_174103.
Phylogenomic databases
PhylomeDB	Q949J1.
ProtClustDB	PLN02373.
Enzyme and pathway databases
BRENDA	3.6.1.1. 1318.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR2_CHLRE

Accession

Primary (citable) accession number: Q949J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	December 1, 2001
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program