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Protein

Glutamate-gated chloride channel

Gene

GluClalpha

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glutamate-gated chloride channel subunit. Glutamate binding triggers a rapidly reversible current, while the anti-helmintic drug ivermectin triggers a permanently open channel configuration.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711GlutamateBy similarity
Binding sitei90 – 901GlutamateBy similarity
Binding sitei154 – 1541GlutamateBy similarity
Binding sitei183 – 1831Glutamate; via carbonyl oxygenBy similarity

GO - Molecular functioni

  • extracellular-glutamate-gated chloride channel activity Source: FlyBase

GO - Biological processi

  • chloride transmembrane transport Source: GOC
  • chloride transport Source: UniProtKB
  • neuron projection morphogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Protein family/group databases

TCDBi1.A.9.4.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-gated chloride channel
Short name:
DrosGluCl
Gene namesi
Name:GluClalpha
Synonyms:GluClImported
ORF Names:CG7535
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0024963. GluClalpha.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 245223ExtracellularBy similarityAdd
BLAST
Transmembranei246 – 26823Helical; Name=1By similarityAdd
BLAST
Topological domaini269 – 2735CytoplasmicBy similarity
Transmembranei274 – 29522Helical; Name=2By similarityAdd
BLAST
Topological domaini296 – 3027ExtracellularBy similarity
Transmembranei303 – 32321Helical; Name=3By similarityAdd
BLAST
Topological domaini324 – 426103CytoplasmicBy similarityAdd
BLAST
Transmembranei427 – 45024Helical; Name=4By similarityAdd
BLAST
Topological domaini451 – 4566ExtracellularBy similarity

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB
  • plasma membrane Source: FlyBase
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 456434Glutamate-gated chloride channelSequence analysisPRO_0000000497Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi163 ↔ 177By similarity
Disulfide bondi222 ↔ 233By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ94900.

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

BgeeiQ94900.
ExpressionAtlasiQ94900. differential.
GenevisibleiQ94900. DM.

Interactioni

Subunit structurei

Pentamer (By similarity). Homomultimer.By similarity1 Publication

Protein-protein interaction databases

BioGridi67340. 2 interactions.
MINTiMINT-813042.
STRINGi7227.FBpp0099473.

Structurei

3D structure databases

ProteinModelPortaliQ94900.
SMRiQ94900. Positions 33-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
InParanoidiQ94900.
KOiK05273.
OrthoDBiEOG712TVZ.
PhylomeDBiQ94900.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR015680. Glu_Cl_Channel.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PTHR18945:SF527. PTHR18945:SF527. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform F (identifier: Q94900-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSGHYFWAI LYFASLCSAS LANNAKINFR EKEKKVLDQI LGAGKYDARI
60 70 80 90 100
RPSGINGTDG PAIVRINLFV RSIMTISDIK MEYSVQLTFR EQWTDERLKF
110 120 130 140 150
DDIQGRLKYL TLTEANRVWM PDLFFSNEKE GHFHNIIMPN VYIRIFPNGS
160 170 180 190 200
VLYSIRISLT LACPMNLKLY PLDRQICSLR MASYGWTTND LVFLWKEGDP
210 220 230 240 250
VQVVKNLHLP RFTLEKFLTD YCNSKTNTGE YSCLKVDLLF KREFSYYLIQ
260 270 280 290 300
IYIPCCMLVI VSWVSFWLDQ GAVPARVSLG VTTLLTMATQ TSGINASLPP
310 320 330 340 350
VSYTKAIDVW TGVCLTFVFG ALLEFALVNY ASRSGSNKAN MHKESMKKKR
360 370 380 390 400
RDLEQASLDA ASDLLDTDSN ATFAMKPLVR HPGDPLALEK RLQCEVHMQA
410 420 430 440 450
PKRPNCCKTW LSKFPTRQCS RSKRIDVISR ITFPLVFALF NLVYWSTYLF

REEEDE
Length:456
Mass (Da):52,307
Last modified:February 1, 2005 - v2
Checksum:iAE892B338B79A060
GO
Isoform A1 Publication (identifier: Q94900-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     230-232: EYS → T

Note: No experimental confirmation available.Curated
Show »
Length:454
Mass (Da):52,028
Checksum:i11F0652393A2A790
GO
Isoform B1 Publication (identifier: Q94900-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-80: LFVRSIMTISDIK → IFVRSISKIDDVT
     417-420: Missing.
     456-456: E → ETF

Note: No experimental confirmation available.
Show »
Length:454
Mass (Da):52,050
Checksum:i949C2186157B1C93
GO
Isoform C (identifier: Q94900-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-79: GPAIVRINLFVRSIMTISDI → NKATNVSVNMFLRSISKIDDY
     417-420: Missing.
     456-456: E → ETF

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):52,281
Checksum:i016EEC25D15F29C2
GO
Isoform D (identifier: Q94900-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-80: LFVRSIMTISDIK → IFVRSISKIDDVT
     335-341: GSNKANM → DV
     456-456: E → ETF

Show »
Length:453
Mass (Da):52,036
Checksum:i09E2F3F0A05242B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501I → V in ABG57261 (Ref. 4) Curated
Sequence conflicti121 – 1211P → S in AAG40736 (PubMed:11095718).Curated
Sequence conflicti379 – 3791V → A in ABG57261 (Ref. 4) Curated

RNA editingi

Edited at positions 27, 63, 66, 241 and 345.5 Publications
Partially edited. Edited by Adar.2 Publications

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti27 – 271I → V in RNA edited version. 2 Publications
Natural varianti63 – 631I → V in RNA edited version. 1 Publication
Natural varianti66 – 661I → V in RNA edited version. 1 Publication
Natural varianti241 – 2411K → R in RNA edited version. 2 Publications
Natural varianti299 – 2991P → S Resistant to nodulisporic acid. 2 Publications
Natural varianti345 – 3451S → N in RNA edited version. 2 Publications

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei60 – 7920GPAIV…TISDI → NKATNVSVNMFLRSISKIDD Y in isoform C. CuratedVSP_026113Add
BLAST
Alternative sequencei68 – 8013LFVRS…ISDIK → IFVRSISKIDDVT in isoform B and isoform D. 3 PublicationsVSP_051644Add
BLAST
Alternative sequencei230 – 2323EYS → T in isoform A. 1 PublicationVSP_051645
Alternative sequencei335 – 3417GSNKANM → DV in isoform D. 1 PublicationVSP_026114
Alternative sequencei417 – 4204Missing in isoform B and isoform C. 2 PublicationsVSP_051646
Alternative sequencei456 – 4561E → ETF in isoform B, isoform C and isoform D. 3 PublicationsVSP_051647

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58776 mRNA. Translation: AAC47266.1.
AJ002232 mRNA. Translation: CAA05260.1.
AF297500 mRNA. Translation: AAG40735.1.
AF297501 mRNA. Translation: AAG40736.1.
DQ665648 mRNA. Translation: ABG57261.1.
AE014297 Genomic DNA. Translation: AAF55695.1.
AE014297 Genomic DNA. Translation: AAN13808.1.
AE014297 Genomic DNA. Translation: AAX52967.1.
AE014297 Genomic DNA. Translation: ABC66182.1.
RefSeqiNP_001014641.2. NM_001014641.4.
NP_001034061.2. NM_001038972.4.
NP_650827.3. NM_142570.4.
NP_732447.2. NM_169873.4.
UniGeneiDm.20644.

Genome annotation databases

GeneIDi42350.
KEGGidme:Dmel_CG7535.
UCSCiCG7535-RA. d. melanogaster. [Q94900-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58776 mRNA. Translation: AAC47266.1.
AJ002232 mRNA. Translation: CAA05260.1.
AF297500 mRNA. Translation: AAG40735.1.
AF297501 mRNA. Translation: AAG40736.1.
DQ665648 mRNA. Translation: ABG57261.1.
AE014297 Genomic DNA. Translation: AAF55695.1.
AE014297 Genomic DNA. Translation: AAN13808.1.
AE014297 Genomic DNA. Translation: AAX52967.1.
AE014297 Genomic DNA. Translation: ABC66182.1.
RefSeqiNP_001014641.2. NM_001014641.4.
NP_001034061.2. NM_001038972.4.
NP_650827.3. NM_142570.4.
NP_732447.2. NM_169873.4.
UniGeneiDm.20644.

3D structure databases

ProteinModelPortaliQ94900.
SMRiQ94900. Positions 33-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67340. 2 interactions.
MINTiMINT-813042.
STRINGi7227.FBpp0099473.

Protein family/group databases

TCDBi1.A.9.4.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Proteomic databases

PaxDbiQ94900.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi42350.
KEGGidme:Dmel_CG7535.
UCSCiCG7535-RA. d. melanogaster. [Q94900-1]

Organism-specific databases

CTDi42350.
FlyBaseiFBgn0024963. GluClalpha.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
InParanoidiQ94900.
KOiK05273.
OrthoDBiEOG712TVZ.
PhylomeDBiQ94900.

Miscellaneous databases

GenomeRNAii42350.
NextBioi828361.
PROiQ94900.

Gene expression databases

BgeeiQ94900.
ExpressionAtlasiQ94900. differential.
GenevisibleiQ94900. DM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR015680. Glu_Cl_Channel.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PTHR18945:SF527. PTHR18945:SF527. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a Drosophila melanogaster glutamate-gated chloride channel sensitive to the antiparasitic agent avermectin."
    Cully D.F., Paress P.S., Liu K.K., Schaeffer J.M., Arena J.P.
    J. Biol. Chem. 271:20187-20191(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM F), FUNCTION, SUBUNIT, DEVELOPMENTAL STAGE, RNA EDITING OF POSITIONS 27; 241 AND 345.
    Strain: Oregon-RImported.
    Tissue: HeadImported.
  2. "Diversification of Drosophila chloride channel gene by multiple posttranscriptional mRNA modifications."
    Semenov E.P., Pak W.L.
    J. Neurochem. 72:66-72(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), VARIANT SER-299, RNA EDITING OF POSITIONS 27; 63; 66 AND 241.
    Tissue: Head1 Publication.
  3. "Drug-resistant Drosophila indicate glutamate-gated chloride channels are targets for the antiparasitics nodulisporic acid and ivermectin."
    Kane N.S., Hirschberg B., Qian S., Hunt D., Thomas B., Brochu R., Ludmerer S.W., Zheng Y., Smith M., Arena J.P., Cohen C.J., Schmatz D., Warmke J., Cully D.F.
    Proc. Natl. Acad. Sci. U.S.A. 97:13949-13954(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM F), NUCLEOTIDE SEQUENCE [MRNA] OF 1-336 (ISOFORM B), FUNCTION, VARIANT SER-299, RNA EDITING OF POSITIONS 27; 63; 66; 241 AND 345.
  4. "Cloning and functional expression of a Drosophila melanogaster glutamate-gated chloride channel."
    Wang X., Xiang W., Wang X., Jiang L., Lin S., Gao A., Wang L., Sun L., Jing B., Xi D.
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D), RNA EDITING OF POSITIONS 27; 63; 66; 241 AND 345.
  5. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], RNA EDITING OF POSITION 63; 66 AND 345.
    Strain: Berkeley1 Publication.
  6. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  7. "A-to-I pre-mRNA editing in Drosophila is primarily involved in adult nervous system function and integrity."
    Palladino M.J., Keegan L.P., O'Connell M.A., Reenan R.A.
    Cell 102:437-449(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA EDITING OF POSITIONS 27; 63; 66; 241 AND 345.

Entry informationi

Entry nameiGLUCL_DROME
AccessioniPrimary (citable) accession number: Q94900
Secondary accession number(s): O77295
, Q0Q0M3, Q2PDQ5, Q59DV9, Q8IN68, Q9GQ52, Q9GQ53, Q9VDU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: May 11, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Channels desensitize rapidly in the continued presence of glutamate and are activated by the glutamate analog ibotenate. In Xenopus oocytes, avermectins and nodulisporic acid directly activate channel conductance.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.