Q948T6 (LGUL_ORYSJ) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 55.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Lactoylglutathione lyase EC=4.4.1.5 Alternative name(s): Aldoketomutase Allergen Glb33 Glyoxalase I Short name=Glx I Ketone-aldehyde mutase Methylglyoxalase PP33 S-D-lactoylglutathione methylglyoxal lyase Allergen=Ory s ? | ||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) | ||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 291 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity. |
| Catalytic activity | (R)-S-lactoylglutathione = glutathione + methylglyoxal. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Pathway | |
| Tissue specificity | Expressed in callus, stem, leaves, panicles and maturing seeds (at protein level). Ref.1 Ref.4 |
| Post-translational modification | Phosphorylated after gibberellin treatment. Ref.5 |
| Allergenic properties | Causes an allergic reaction in human. Binds to IgE. Ref.1 |
| Sequence similarities | Belongs to the glyoxalase I family. |
| Biophysicochemical properties | Kinetic parameters: KM=7.6 mM for methylglyoxal Ref.1 |
Ontologies
| Keywords | |
|---|---|
| Disease | Allergen |
| Ligand | Metal-binding Zinc |
| Molecular function | Lyase |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular function | lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 291 | 291 | Lactoylglutathione lyase | PRO_0000168085 | |||||
Sites | |||||||||
| Metal binding | 27 | 1 | Zinc By similarity | ||||||
| Metal binding | 78 | 1 | Zinc By similarity | ||||||
| Metal binding | 96 | 1 | Zinc By similarity | ||||||
| Metal binding | 145 | 1 | Zinc By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 25 | 1 | L → V in BAB71741. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A 33-kDa allergen from rice (Oryza sativa L. Japonica). cDNA cloning, expression, and identification as a novel glyoxalase I." Usui Y., Nakase M., Hotta H., Urisu A., Aoki N., Kitajima K., Matsuda T. J. Biol. Chem. 276:11376-11381(2001) [PubMed: 11139585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 222-236 AND 267-277, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, ALLERGEN. |
| [2] | "The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [3] | "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare. |
| [4] | "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-37, TISSUE SPECIFICITY. Strain: cv. Nipponbare. Tissue: Callus and Panicle. |
| [5] | "Identification of phosphoproteins regulated by gibberellin in rice leaf sheath." Khan M.M.K., Jan A., Karibe H., Komatsu S. Plant Mol. Biol. 58:27-40(2005) [PubMed: 16028114] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, PHOSPHORYLATION. Strain: cv. Nipponbare. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB017042 mRNA. Translation: BAA36759.1. AB050986 Genomic DNA. Translation: BAB71741.1. AP005441 Genomic DNA. Translation: BAD05593.1. AK066092 mRNA. No translation available. AK103694 mRNA. No translation available. |
| RefSeq | NP_001061172.1. NM_001067707.1. |
| UniGene | Os.7835. |
3D structure databases | |
| ProteinModelPortal | Q948T6. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q948T6. |
Protein family/group databases | |
| Allergome | 1048. Ory s Glyoxalase I. |
2D gel databases | |
| ANU-2DPAGE | Q948T6. |
Proteomic databases | |
| PRIDE | Q948T6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | LOC_Os08g09250.2; LOC_Os08g09250.2; LOC_Os08g09250. |
| GeneID | 4344858. |
| KEGG | osa:4344858. |
Organism-specific databases | |
| Gramene | Q948T6. |
Phylogenomic databases | |
| GeneTree | EPGT00050000015867. |
| PhylomeDB | Q948T6. |
| ProtClustDB | PLN02300. |
Family and domain databases | |
| InterPro | IPR004360. Glyas_Fos-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view] |
| KO | K01759. |
| Pfam | PF00903. Glyoxalase. 2 hits. [Graphical view] |
| TIGRFAMs | TIGR00068. Glyox_I. 2 hits. |
| PROSITE | PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LGUL_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: Q948T6 Secondary accession number(s): Q9ZWJ2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Allergens Nomenclature of allergens and list of entries |
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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