Q948T6 (LGUL_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactoylglutathione lyase
EC=4.4.1.5

Alternative name(s):
Aldoketomutase
Allergen Glb33
Glyoxalase I
Short name=Glx I
Ketone-aldehyde mutase
Methylglyoxalase
PP33
S-D-lactoylglutathione methylglyoxal lyase
Allergen=Ory s ?

Gene names

Name:	GLX-I
Ordered Locus Names:	Os08g0191700, LOC_Os08g09250
ORF Names:	OSJNBa0056O06.9-1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Tissue specificity	Expressed in callus, stem, leaves, panicles and maturing seeds (at protein level). Ref.1 Ref.4
Post-translational modification	Phosphorylated after gibberellin treatment. Ref.5
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Ref.1
Sequence similarities	Belongs to the glyoxalase I family.
Biophysicochemical properties	Kinetic parameters: KM=7.6 mM for methylglyoxal Ref.1

Ontologies

Keywords
Disease	Allergen
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Lactoylglutathione lyase		PRO_0000168085
Sites
	Metal binding	27	1	Zinc By similarity
	Metal binding	78	1	Zinc By similarity
	Metal binding	96	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
Experimental info
	Sequence conflict	25	1	L → V in BAB71741. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q948T6 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 5CB9FEB32F2BC7ED	FASTA	291	32,553
	10 20 30 40 50 60 MASGSEAEKS PEVVLEWPKK DKKRLLHAVY RVGDLDRTIK CYTECFGMKL LRKRDVPEEK 70 80 90 100 110 120 YTNAFLGFGP EDTNFALELT YNYGVDKYDI GAGFGHFAIA TEDVYKLAEK IKSSCCCKIT 130 140 150 160 170 180 REPGPVKGGS TVIAFAQDPD GYMFELIQRG PTPEPLCQVM LRVGDLDRSI KFYEKALGMK 190 200 210 220 230 240 LLRKKDVPDY KYTIAMLGYA DEDKTTVIEL TYNYGVTEYT KGNAYAQVAI GTEDVYKSAE 250 260 270 280 290 AVELVTKELG GKILRQPGPL PGLNTKIASF LDPDGWKVVL VDNADFLKEL Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 33-kDa allergen from rice (Oryza sativa L. Japonica). cDNA cloning, expression, and identification as a novel glyoxalase I." Usui Y., Nakase M., Hotta H., Urisu A., Aoki N., Kitajima K., Matsuda T. J. Biol. Chem. 276:11376-11381(2001) [PubMed: 11139585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 222-236 AND 267-277, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, ALLERGEN.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-37, TISSUE SPECIFICITY. Strain: cv. Nipponbare. Tissue: Callus and Panicle.
[5]	"Identification of phosphoproteins regulated by gibberellin in rice leaf sheath." Khan M.M.K., Jan A., Karibe H., Komatsu S. Plant Mol. Biol. 58:27-40(2005) [PubMed: 16028114] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, PHOSPHORYLATION. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017042 mRNA. Translation: BAA36759.1. AB050986 Genomic DNA. Translation: BAB71741.1. AP005441 Genomic DNA. Translation: BAD05593.1. AK066092 mRNA. No translation available. AK103694 mRNA. No translation available.
RefSeq	NP_001061172.1. NM_001067707.1.
UniGene	Os.7835.
3D structure databases
ProteinModelPortal	Q948T6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q948T6.
Protein family/group databases
Allergome	1048. Ory s Glyoxalase I.
2D gel databases
ANU-2DPAGE	Q948T6.
Proteomic databases
PRIDE	Q948T6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g09250.2; LOC_Os08g09250.2; LOC_Os08g09250.
GeneID	4344858.
KEGG	osa:4344858.
Organism-specific databases
Gramene	Q948T6.
Phylogenomic databases
GeneTree	EPGT00050000015867.
PhylomeDB	Q948T6.
ProtClustDB	PLN02300.
Family and domain databases
InterPro	IPR004360. Glyas_Fos-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
KO	K01759.
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00068. Glyox_I. 2 hits.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_ORYSJ

Accession

Primary (citable) accession number: Q948T6
Secondary accession number(s): Q9ZWJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	November 16, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program