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Q948R6 (IMFS_LUFAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isomultiflorenol synthase

Short name=LcIMS1
EC=5.4.99.36
Gene names
Name:IMS1
OrganismLuffa aegyptiaca (Sponge gourd) (Luffa cylindrica)
Taxonomic identifier3670 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeSicyoeaeLuffa

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oxidosqualene cyclase involved in the biosynthesis of bryonolic acid. Converts oxidosqualene to isomultiflorenol. Ref.1

Catalytic activity

(3S)-2,3-epoxy-2,3-dihydrosqualene = isomultiflorenol. Ref.1

Miscellaneous

In cultured cells, IMS1 mRNA expression is high between days 16 and 20 and precedes the accumulation of bryonolic acid.

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 4 PFTB repeats.

Ontologies

Keywords
   DomainRepeat
   Molecular functionIsomerase
Gene Ontology (GO)
   Molecular_functionintramolecular transferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759Isomultiflorenol synthase
PRO_0000412991

Regions

Repeat148 – 18942PFTB 1
Repeat588 – 62841PFTB 2
Repeat637 – 67842PFTB 3
Repeat699 – 74345PFTB 4

Sequences

Sequence LengthMass (Da)Tools
Q948R6 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 8215E88FB3A43359

FASTA75987,840
        10         20         30         40         50         60 
MWRLKVADGG NDPYIYSMNN FIGRQIWEFD PNAGTPEERA EIERLRHHFT KNRHKGFPSA 

        70         80         90        100        110        120 
DLLWRVQLLR EKNFKQSIPA VKVGDGEEIS YEMALDAMRR GAHFLAAIQA SDGHWPSETS 

       130        140        150        160        170        180 
GPLFYVCPLL ICMYIMGFMD KVFSPEHKKE MMRYIYNHQN EDGGWGLHVG GHSNMFCTTF 

       190        200        210        220        230        240 
NYISLRLLGE EPDVEAVCKA RNWIHDHDGV TSILSWGKTW LSILNVFDWS ASNPMPPEYW 

       250        260        270        280        290        300 
MLPTWVPIHP SNMMCYTRIT YMPMSYLYGK RFQAPLTPLV LQLRDELHTQ PYDQINWRKV 

       310        320        330        340        350        360 
RHMCATEDLY FPHPFVQDLL WDTLYLLSEP LMTRWPFNKL IRQKALNETM RHIHYEDENS 

       370        380        390        400        410        420 
RYITIGCVEK PLCMLACWVE DPNSEYVKKH LARIPDYLWM AEDGMKMQSF GSQSWDAALA 

       430        440        450        460        470        480 
MQALLSCNIT REIGSVLNSG HDFIKNSQVR NNPPGDYKSM FRYMSKGSWT FSDCDHGWQV 

       490        500        510        520        530        540 
SDCTAENLKC CLLLSLLPPD IVGEKMEPER FYDAVNVILN MQSKNGGLPA WEPASSYYWM 

       550        560        570        580        590        600 
EWLNPVEFLE DLIIEHQHVE CTSSALQAIL LFRKQYPGHR RKEINNFINK AVQFLQDIQL 

       610        620        630        640        650        660 
PDGSWYGNWG ICYTYGTWFA LKALSMAGKT YENCEAVRKG ANFLRKIQNP EGGFGESYLS 

       670        680        690        700        710        720 
CPYKRYIPLD GKRSNLVQTA WGMMGLICAG QADVDPTPIH RAAKLLINSQ TEDGDFPQEE 

       730        740        750 
ITGEFFKNCT LHFAAFREVF PVMALGEYCN KVPLPSKKK 

« Hide

References

[1]"Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid."
Hayashi H., Huang P., Inoue K., Hiraoka N., Ikeshiro Y., Yazaki K., Tanaka S., Kushiro T., Shibuya M., Ebizuka Y.
Eur. J. Biochem. 268:6311-6317(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB058643 mRNA. Translation: BAB68529.1.

3D structure databases

ProteinModelPortalQ948R6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01787. squalene_cyclas. 1 hit.
PROSITEPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIMFS_LUFAE
AccessionPrimary (citable) accession number: Q948R6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families