Q948P5 (FRI4_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ferritin-4, chloroplastic EC=1.16.3.1 Alternative name(s): SFerH-4
Organism	Glycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation By similarity.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Subunit structure	Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferritin family. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 40

40

Chloroplast Potential

Chain

41 – 247

207

Ferritin-4, chloroplastic

PRO_0000008867

Regions

Domain

74 – 227

154

Ferritin-like diiron

Region

41 – 73

33

Extension peptide (EP)

Sites

Metal binding

91

1

Iron 1 By similarity

Metal binding

126

1

Iron 1 By similarity

Metal binding

126

1

Iron 2 By similarity

Metal binding

129

1

Iron 1 By similarity

Metal binding

175

1

Iron 2 By similarity

Metal binding

209

1

Iron 2 By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

247

Helix Strand Turn

Details...

Helix

52 – 56

5

Turn

57 – 62

6

Helix

78 – 105

28

Turn

108 – 110

3

Helix

113 – 140

28

Turn

159 – 161

3

Helix

163 – 191

29

Helix

195 – 204

10

Helix

206 – 226

21

Helix

230 – 240

11

Sequences

	Sequence		Length	Mass (Da)
	Q948P5 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 9A643DDFB5DDEDE3	FASTA	247	27,559
	10 20 30 40 50 60 MLLRTAAASA SSLSLFSPNA EPPRSVPARG LVVRAAKGST NHRALTGVIF EPFEEVKKEL 70 80 90 100 110 120 DLVPTVPQAS LARQKYVDES ESAVNEQINV EYNVSYVYHA MFAYFDRDNV ALRGLAKFFK 130 140 150 160 170 180 ESSEEEREHA EKLMEYQNKR GGKVKLQSIV MPLSDFDHAD KGDALHAMEL ALSLEKLTNE 190 200 210 220 230 240 KLLNLHSVAT KNGDVQLADF VETEYLGEQV EAIKRISEYV AQLRRVGKGH GVWHFDQMLL HEGGDAA

References

[1]	"Construction of homo- and heteropolymers of plant ferritin subunits using an in vitro protein expression system." Masuda T., Goto F., Yoshihara T., Ezure T., Suzuki T., Kobayashi S., Shikata M., Utsumi S. Protein Expr. Purif. 56:237-246(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB062756 mRNA. Translation: BAB64537.2.

RefSeq

NP_001237049.1. NM_001250120.1.

UniGene

Gma.18301.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3A68	X-ray	1.80	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X	36-247	[»]
3A9Q	X-ray	1.90	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X	36-247	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

GLYMA14G06160.1; GLYMA14G06160.1; GLYMA14G06160.

GeneID

547477.

KEGG

gmx:547477.

Gene expression databases

Genevestigator

Q948P5.

Family and domain databases

Gene3D

1.20.1260.10. 1 hit.

InterPro

IPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR014034. Ferritin_CS.
IPR008331. Ferritin_DPS_dom.
[Graphical view]

PANTHER

PTHR11431. PTHR11431. 1 hit.

Pfam

PF00210. Ferritin. 1 hit.
[Graphical view]

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

PROSITE

PS00540. FERRITIN_1. False negative.
PS00204. FERRITIN_2. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q948P5.

Entry information

Entry name

FRI4_SOYBN

Accession

Primary (citable) accession number: Q948P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program