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Protein

Neurexin-4

Gene

Nrx-IV

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to play a role in the formation and function of septate junctions. Septate junctions, which are the equivalent of vertebrates tight junctions, are characterized by regular arrays of transverse structures that span the intermembrane space and form a physical barrier to diffusion. Required for the blood-brain barrier formation.1 Publication

GO - Biological processi

  • axon ensheathment Source: FlyBase
  • cell adhesion involved in heart morphogenesis Source: FlyBase
  • cell-cell junction organization Source: FlyBase
  • dorsal closure Source: FlyBase
  • establishment of glial blood-brain barrier Source: FlyBase
  • establishment or maintenance of cell polarity Source: FlyBase
  • heart process Source: FlyBase
  • nerve maturation Source: FlyBase
  • presynaptic membrane assembly Source: BHF-UCL
  • protein localization Source: FlyBase
  • regulation of tube size, open tracheal system Source: FlyBase
  • septate junction assembly Source: FlyBase
  • synaptic target recognition Source: FlyBase
  • synaptic vesicle docking Source: FlyBase
  • synaptic vesicle targeting Source: FlyBase
  • terminal button organization Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neurexin-4
Alternative name(s):
Neurexin IV
Gene namesi
Name:Nrx-IV
Synonyms:Nrx
ORF Names:CG6827
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0013997. Nrx-IV.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 1217ExtracellularSequence analysisAdd BLAST1182
Transmembranei1218 – 1238HelicalSequence analysisAdd BLAST21
Topological domaini1239 – 1284CytoplasmicSequence analysisAdd BLAST46

GO - Cellular componenti

  • integral component of plasma membrane Source: FlyBase
  • plasma membrane Source: FlyBase
  • pleated septate junction Source: FlyBase
  • presynaptic active zone Source: BHF-UCL
  • septate junction Source: FlyBase
  • synapse Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000001951236 – 1284Neurexin-4Add BLAST1249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 185By similarity
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi333 ↔ 369By similarity
Glycosylationi340N-linked (GlcNAc...)Sequence analysis1
Glycosylationi398N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi507 ↔ 540By similarity
Disulfide bondi546 ↔ 557By similarity
Disulfide bondi551 ↔ 566By similarity
Disulfide bondi568 ↔ 578By similarity
Glycosylationi668N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi934 ↔ 962By similarity
Disulfide bondi966 ↔ 977By similarity
Disulfide bondi971 ↔ 986By similarity
Glycosylationi974N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi988 ↔ 998By similarity
Glycosylationi1047N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1137N-linked (GlcNAc...)1 Publication1
Disulfide bondi1147 ↔ 1183By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ94887.
PRIDEiQ94887.

Expressioni

Tissue specificityi

Found in septate junctions of epithelial and glial cells.1 Publication

Gene expression databases

BgeeiFBgn0013997.
ExpressionAtlasiQ94887. baseline.
GenevisibleiQ94887. DM.

Interactioni

Subunit structurei

The C-terminal region interacts with coracle. Interacts with Patj in cis form. Forms a complex with Nrg and Cont.3 Publications

Protein-protein interaction databases

BioGridi64741. 5 interactors.
IntActiQ94887. 30 interactors.
STRINGi7227.FBpp0075730.

Structurei

3D structure databases

ProteinModelPortaliQ94887.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 185F5/8 type CPROSITE-ProRule annotationAdd BLAST139
Domaini220 – 369Laminin G-like 1PROSITE-ProRule annotationAdd BLAST150
Domaini403 – 540Laminin G-like 2PROSITE-ProRule annotationAdd BLAST138
Domaini542 – 579EGF-like 1PROSITE-ProRule annotationAdd BLAST38
Domaini824 – 962Laminin G-like 3PROSITE-ProRule annotationAdd BLAST139
Domaini962 – 999EGF-like 2PROSITE-ProRule annotationAdd BLAST38
Domaini1032 – 1183Laminin G-like 4PROSITE-ProRule annotationAdd BLAST152

Sequence similaritiesi

Belongs to the neurexin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation
Contains 4 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3516. Eukaryota.
ENOG410XPHG. LUCA.
GeneTreeiENSGT00760000118991.
InParanoidiQ94887.
OMAiMYDANQL.
OrthoDBiEOG091G00LF.
PhylomeDBiQ94887.

Family and domain databases

Gene3Di2.60.120.200. 4 hits.
2.60.120.260. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00754. F5_F8_type_C. 1 hit.
PF02210. Laminin_G_2. 4 hits.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00181. EGF. 2 hits.
SM00231. FA58C. 1 hit.
SM00282. LamG. 4 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 5 hits.
PROSITEiPS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
PS50025. LAM_G_DOMAIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPRSNTKA AFSSLQFGLL CLLLLVNNGI KSVQADAFTD YFSDYDCNQP
60 70 80 90 100
LMERAVLTAT SSLTERGPDK ARLNGNAAWT PVENTYNHFL TLDLGDPRMV
110 120 130 140 150
RKIATMGRMH TDEFVTEYIV QYSDDGEFWR SYVNPTSEPQ MFKGNSDGNS
160 170 180 190 200
IHYNVFEVPI IAQWVRINPT RWHDRISMRV ELYGCDYISE NLYFNGTGLV
210 220 230 240 250
RYDLRREPIT STKESIRFRF KTAFANGVMM YSRGTQGDYY ALQLKDNKMV
260 270 280 290 300
LNLDLGSRVM TSLSVGSLLD DNVWHDVVIS RNQRDIIFSV DRVIVRGRIQ
310 320 330 340 350
GEFTRLNLNR ELYLGGVPNV QEGLIVQQNF SGCLENIYFN STNFIRVMKD
360 370 380 390 400
STELGEGYLF TRVNTIYACP SPPIYPVTFT TRSSFVRLKG YENSQRLNVS
410 420 430 440 450
FYFRTYEETG VMLHHDFYSG GYLKVFLEFG KVKIDLKVKD KARIILDNYD
460 470 480 490 500
DQFNDGKWHS FVISIEKNRL ILNIDQRPMT TTKSMQVATG AQYYIAGGKD
510 520 530 540 550
KNGFVGCMRL ISVDGNYKLP QDWVKGEEVC CGDDVVVDAC QMIDRCNPNP
560 570 580 590 600
CQHKGLCHQN SREFFCDCGH TGYAGAVCHT SNNPLSCLAL KNVQHVQQRV
610 620 630 640 650
NLNLDVDGSG PLEPFPVTCE FYSDGRVITT LSHSQEHTTT VDGFQEPGSF
660 670 680 690 700
EQSIMYDANQ LQIEALLNRS HSCWQRLSYS CRSSRLFNSP SEAGNFRPFS
710 720 730 740 750
WWISRHNQPM DYWAGALPGS RKCECGILGK CHDPTKWCNC DSNSLEWMED
760 770 780 790 800
GGDIREKEYL PVRAVKFGDT GTPLDEKMGR YTLGPLRCEG DDLFSNVVTF
810 820 830 840 850
RIADASINLP PFDMGHSGDI YLEFRTTQEN SVIFHATGPT DYIKLSLNGG
860 870 880 890 900
NKLQFQYQAG SGPLGVNVGT SYHLNDNNWH TVSVERNRKE ARLVVDGSIK
910 920 930 940 950
AEVREPPGPV RALHLTSDLV IGATTEYRDG YVGCIRALLL NGKMVDLKEY
960 970 980 990 1000
SKRGLYGIST GCVGRCESNP CLNNGTCIER YDGYSCDCRW SAFKGPICAD
1010 1020 1030 1040 1050
EIGVNLRSSS IIRYEFEGSF RSTIAENIRV GFTTTIPKGF LLGFSSNLTG
1060 1070 1080 1090 1100
EYLTIQISNS GHLRCVFDFG FERQEIIFPK KHFGLGQYHD MHFMRKNGGS
1110 1120 1130 1140 1150
TVVLKVDNYE PVEYNFDIKA SADAQFNNIQ YMYIGKNESM TDGFVGCVSR
1160 1170 1180 1190 1200
VQFDDIYPLK LMFQQNPPKN VKSLGTQLTE DFCGVEPVTH PPIEIETRPP
1210 1220 1230 1240 1250
PLVDEEKLRK AYNEVDSVLL ACLLVILFLL LILMFFLIGR YLHRHKGDYL
1260 1270 1280
THEDQGADGA DDPDDAVLHS TTGHQVRKRT EIFI
Length:1,284
Mass (Da):145,468
Last modified:December 5, 2001 - v2
Checksum:i9372C71AC70E3D56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 4MRPP → MSA (PubMed:8978610).Curated4
Sequence conflicti926E → D in CAA60383 (PubMed:8978610).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86685 mRNA. Translation: CAA60383.1.
AE014296 Genomic DNA. Translation: AAF49951.1.
PIRiT13799.
RefSeqiNP_524034.2. NM_079310.3.
UniGeneiDm.1843.

Genome annotation databases

EnsemblMetazoaiFBtr0075998; FBpp0075730; FBgn0013997.
GeneIDi39387.
KEGGidme:Dmel_CG6827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86685 mRNA. Translation: CAA60383.1.
AE014296 Genomic DNA. Translation: AAF49951.1.
PIRiT13799.
RefSeqiNP_524034.2. NM_079310.3.
UniGeneiDm.1843.

3D structure databases

ProteinModelPortaliQ94887.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64741. 5 interactors.
IntActiQ94887. 30 interactors.
STRINGi7227.FBpp0075730.

Proteomic databases

PaxDbiQ94887.
PRIDEiQ94887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075998; FBpp0075730; FBgn0013997.
GeneIDi39387.
KEGGidme:Dmel_CG6827.

Organism-specific databases

CTDi39387.
FlyBaseiFBgn0013997. Nrx-IV.

Phylogenomic databases

eggNOGiKOG3516. Eukaryota.
ENOG410XPHG. LUCA.
GeneTreeiENSGT00760000118991.
InParanoidiQ94887.
OMAiMYDANQL.
OrthoDBiEOG091G00LF.
PhylomeDBiQ94887.

Miscellaneous databases

GenomeRNAii39387.
PROiQ94887.

Gene expression databases

BgeeiFBgn0013997.
ExpressionAtlasiQ94887. baseline.
GenevisibleiQ94887. DM.

Family and domain databases

Gene3Di2.60.120.200. 4 hits.
2.60.120.260. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00754. F5_F8_type_C. 1 hit.
PF02210. Laminin_G_2. 4 hits.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00181. EGF. 2 hits.
SM00231. FA58C. 1 hit.
SM00282. LamG. 4 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 5 hits.
PROSITEiPS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
PS50025. LAM_G_DOMAIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRX4_DROME
AccessioniPrimary (citable) accession number: Q94887
Secondary accession number(s): Q9VTU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: December 5, 2001
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.