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Q94887

- NRX4_DROME

UniProt

Q94887 - NRX4_DROME

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Protein

Neurexin-4

Gene

Nrx-IV

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Seems to play a role in the formation and function of septate junctions. Septate junctions, which are the equivalent of vertebrates tight junctions, are characterized by regular arrays of transverse structures that span the intermembrane space and form a physical barrier to diffusion. Required for the blood-brain barrier formation.1 Publication

GO - Biological processi

  1. axon ensheathment Source: FlyBase
  2. cell adhesion involved in heart morphogenesis Source: FlyBase
  3. cell-cell junction organization Source: FlyBase
  4. dorsal closure Source: FlyBase
  5. establishment of glial blood-brain barrier Source: FlyBase
  6. establishment or maintenance of cell polarity Source: FlyBase
  7. heart process Source: FlyBase
  8. nerve maturation Source: FlyBase
  9. presynaptic membrane assembly Source: BHF-UCL
  10. protein localization Source: FlyBase
  11. regulation of tube size, open tracheal system Source: FlyBase
  12. septate junction assembly Source: FlyBase
  13. synaptic target recognition Source: FlyBase
  14. synaptic vesicle docking involved in exocytosis Source: FlyBase
  15. synaptic vesicle targeting Source: FlyBase
  16. terminal button organization Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neurexin-4
Alternative name(s):
Neurexin IV
Gene namesi
Name:Nrx-IV
Synonyms:Nrx
ORF Names:CG6827
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0013997. Nrx-IV.

Subcellular locationi

GO - Cellular componenti

  1. integral component of plasma membrane Source: FlyBase
  2. plasma membrane Source: FlyBase
  3. pleated septate junction Source: FlyBase
  4. presynaptic active zone Source: BHF-UCL
  5. septate junction Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Chaini36 – 12841249Neurexin-4PRO_0000019512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 185By similarity
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi333 ↔ 369By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi507 ↔ 540By similarity
Disulfide bondi546 ↔ 557By similarity
Disulfide bondi551 ↔ 566By similarity
Disulfide bondi568 ↔ 578By similarity
Glycosylationi668 – 6681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi934 ↔ 962By similarity
Disulfide bondi966 ↔ 977By similarity
Disulfide bondi971 ↔ 986By similarity
Glycosylationi974 – 9741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi988 ↔ 998By similarity
Glycosylationi1047 – 10471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1137 – 11371N-linked (GlcNAc...)1 Publication
Disulfide bondi1147 ↔ 1183By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ94887.
PRIDEiQ94887.

Expressioni

Tissue specificityi

Found in septate junctions of epithelial and glial cells.1 Publication

Gene expression databases

BgeeiQ94887.

Interactioni

Subunit structurei

The C-terminal region interacts with coracle. Interacts with Patj in cis form. Forms a complex with Nrg and Cont.3 Publications

Protein-protein interaction databases

BioGridi64741. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ94887.
SMRiQ94887. Positions 61-586, 721-747, 775-1154.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 12171182ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1239 – 128446CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1218 – 123821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 185139F5/8 type CPROSITE-ProRule annotationAdd
BLAST
Domaini220 – 369150Laminin G-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini403 – 540138Laminin G-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini542 – 57938EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini824 – 962139Laminin G-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini962 – 99938EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini1032 – 1183152Laminin G-like 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the neurexin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation
Contains 4 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG291100.
GeneTreeiENSGT00760000118991.
InParanoidiQ94887.
OMAiIDRCMPN.
OrthoDBiEOG7GXP9N.
PhylomeDBiQ94887.

Family and domain databases

Gene3Di2.60.120.200. 4 hits.
2.60.120.260. 1 hit.
InterProiIPR000421. Coagulation_fac_5/8-C_type_dom.
IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR008979. Galactose-bd-like.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF02210. Laminin_G_2. 4 hits.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00181. EGF. 2 hits.
SM00231. FA58C. 1 hit.
SM00282. LamG. 4 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 5 hits.
PROSITEiPS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
PS50025. LAM_G_DOMAIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q94887-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRPPRSNTKA AFSSLQFGLL CLLLLVNNGI KSVQADAFTD YFSDYDCNQP
60 70 80 90 100
LMERAVLTAT SSLTERGPDK ARLNGNAAWT PVENTYNHFL TLDLGDPRMV
110 120 130 140 150
RKIATMGRMH TDEFVTEYIV QYSDDGEFWR SYVNPTSEPQ MFKGNSDGNS
160 170 180 190 200
IHYNVFEVPI IAQWVRINPT RWHDRISMRV ELYGCDYISE NLYFNGTGLV
210 220 230 240 250
RYDLRREPIT STKESIRFRF KTAFANGVMM YSRGTQGDYY ALQLKDNKMV
260 270 280 290 300
LNLDLGSRVM TSLSVGSLLD DNVWHDVVIS RNQRDIIFSV DRVIVRGRIQ
310 320 330 340 350
GEFTRLNLNR ELYLGGVPNV QEGLIVQQNF SGCLENIYFN STNFIRVMKD
360 370 380 390 400
STELGEGYLF TRVNTIYACP SPPIYPVTFT TRSSFVRLKG YENSQRLNVS
410 420 430 440 450
FYFRTYEETG VMLHHDFYSG GYLKVFLEFG KVKIDLKVKD KARIILDNYD
460 470 480 490 500
DQFNDGKWHS FVISIEKNRL ILNIDQRPMT TTKSMQVATG AQYYIAGGKD
510 520 530 540 550
KNGFVGCMRL ISVDGNYKLP QDWVKGEEVC CGDDVVVDAC QMIDRCNPNP
560 570 580 590 600
CQHKGLCHQN SREFFCDCGH TGYAGAVCHT SNNPLSCLAL KNVQHVQQRV
610 620 630 640 650
NLNLDVDGSG PLEPFPVTCE FYSDGRVITT LSHSQEHTTT VDGFQEPGSF
660 670 680 690 700
EQSIMYDANQ LQIEALLNRS HSCWQRLSYS CRSSRLFNSP SEAGNFRPFS
710 720 730 740 750
WWISRHNQPM DYWAGALPGS RKCECGILGK CHDPTKWCNC DSNSLEWMED
760 770 780 790 800
GGDIREKEYL PVRAVKFGDT GTPLDEKMGR YTLGPLRCEG DDLFSNVVTF
810 820 830 840 850
RIADASINLP PFDMGHSGDI YLEFRTTQEN SVIFHATGPT DYIKLSLNGG
860 870 880 890 900
NKLQFQYQAG SGPLGVNVGT SYHLNDNNWH TVSVERNRKE ARLVVDGSIK
910 920 930 940 950
AEVREPPGPV RALHLTSDLV IGATTEYRDG YVGCIRALLL NGKMVDLKEY
960 970 980 990 1000
SKRGLYGIST GCVGRCESNP CLNNGTCIER YDGYSCDCRW SAFKGPICAD
1010 1020 1030 1040 1050
EIGVNLRSSS IIRYEFEGSF RSTIAENIRV GFTTTIPKGF LLGFSSNLTG
1060 1070 1080 1090 1100
EYLTIQISNS GHLRCVFDFG FERQEIIFPK KHFGLGQYHD MHFMRKNGGS
1110 1120 1130 1140 1150
TVVLKVDNYE PVEYNFDIKA SADAQFNNIQ YMYIGKNESM TDGFVGCVSR
1160 1170 1180 1190 1200
VQFDDIYPLK LMFQQNPPKN VKSLGTQLTE DFCGVEPVTH PPIEIETRPP
1210 1220 1230 1240 1250
PLVDEEKLRK AYNEVDSVLL ACLLVILFLL LILMFFLIGR YLHRHKGDYL
1260 1270 1280
THEDQGADGA DDPDDAVLHS TTGHQVRKRT EIFI
Length:1,284
Mass (Da):145,468
Last modified:December 5, 2001 - v2
Checksum:i9372C71AC70E3D56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 44MRPP → MSA(PubMed:8978610)Curated
Sequence conflicti926 – 9261E → D in CAA60383. (PubMed:8978610)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86685 mRNA. Translation: CAA60383.1.
AE014296 Genomic DNA. Translation: AAF49951.1.
PIRiT13799.
RefSeqiNP_524034.2. NM_079310.3.
UniGeneiDm.1843.

Genome annotation databases

EnsemblMetazoaiFBtr0075998; FBpp0075730; FBgn0013997.
GeneIDi39387.
KEGGidme:Dmel_CG6827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86685 mRNA. Translation: CAA60383.1 .
AE014296 Genomic DNA. Translation: AAF49951.1 .
PIRi T13799.
RefSeqi NP_524034.2. NM_079310.3.
UniGenei Dm.1843.

3D structure databases

ProteinModelPortali Q94887.
SMRi Q94887. Positions 61-586, 721-747, 775-1154.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 64741. 5 interactions.

Proteomic databases

PaxDbi Q94887.
PRIDEi Q94887.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0075998 ; FBpp0075730 ; FBgn0013997 .
GeneIDi 39387.
KEGGi dme:Dmel_CG6827.

Organism-specific databases

CTDi 39387.
FlyBasei FBgn0013997. Nrx-IV.

Phylogenomic databases

eggNOGi NOG291100.
GeneTreei ENSGT00760000118991.
InParanoidi Q94887.
OMAi IDRCMPN.
OrthoDBi EOG7GXP9N.
PhylomeDBi Q94887.

Miscellaneous databases

GenomeRNAii 39387.
NextBioi 813387.
PROi Q94887.

Gene expression databases

Bgeei Q94887.

Family and domain databases

Gene3Di 2.60.120.200. 4 hits.
2.60.120.260. 1 hit.
InterProi IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR008979. Galactose-bd-like.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
[Graphical view ]
Pfami PF00754. F5_F8_type_C. 1 hit.
PF02210. Laminin_G_2. 4 hits.
[Graphical view ]
SMARTi SM00294. 4.1m. 1 hit.
SM00181. EGF. 2 hits.
SM00231. FA58C. 1 hit.
SM00282. LamG. 4 hits.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 5 hits.
PROSITEi PS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
PS50025. LAM_G_DOMAIN. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Drosophila neurexin is required for septate junction and blood-nerve barrier formation and function."
    Baumgartner S.W., Littleton J.T., Broadie K., Bhat M.A., Harbecke R., Lengyel J.A., Chiquet-Ehrismann R., Prokop A., Bellen H.J.
    Cell 87:1059-1068(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Canton-S.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A conserved functional domain of Drosophila coracle is required for localization at the septate junction and has membrane-organizing activity."
    Ward R.E. IV, Lamb R.S., Fehon R.G.
    J. Cell Biol. 140:1463-1473(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CORACLE.
  5. "Discs Lost, a novel multi-PDZ domain protein, establishes and maintains epithelial polarity."
    Bhat M.A., Izaddoost S., Lu Y., Cho K.-O., Choi K.-W., Bellen H.J.
    Cell 96:833-845(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PATJ.
  6. Erratum
    Bhat M.A., Izaddoost S., Lu Y., Cho K.-O., Choi K.-W., Bellen H.J.
    Cell 115:765-766(2003)
  7. "Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function."
    Faivre-Sarrailh C., Banerjee S., Li J., Hortsch M., Laval M., Bhat M.A.
    Development 131:4931-4942(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH NRX AND CONT.
  8. "Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster."
    Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V.
    Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1137, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Oregon-R.
    Tissue: Head.

Entry informationi

Entry nameiNRX4_DROME
AccessioniPrimary (citable) accession number: Q94887
Secondary accession number(s): Q9VTU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: December 5, 2001
Last modified: October 29, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3