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Q94702

- PPO1_PHYPO

UniProt

Q94702 - PPO1_PHYPO

Protein

Intron-encoded endonuclease I-PpoI

Gene
N/A
Organism
Physarum polycephalum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.

    Cofactori

    Zinc.

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW

    GO - Biological processi

    1. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Intron homing

    Keywords - Ligandi

    Zinc

    Protein family/group databases

    REBASEi2469. I-PpoI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intron-encoded endonuclease I-PpoI (EC:3.1.-.-)
    Short name:
    I-Ppo
    OrganismiPhysarum polycephalum (Slime mold)
    Taxonomic identifieri5791 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaMyxogastriaMyxogastromycetidaePhysariidaPhysaraceaePhysarum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 163163Intron-encoded endonuclease I-PpoIPRO_0000192788Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    163
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1915
    Beta strandi24 – 329
    Beta strandi35 – 439
    Beta strandi46 – 483
    Beta strandi54 – 585
    Beta strandi61 – 688
    Beta strandi71 – 766
    Turni77 – 793
    Helixi80 – 823
    Beta strandi83 – 853
    Beta strandi87 – 893
    Beta strandi92 – 987
    Helixi108 – 1103
    Beta strandi111 – 1155
    Helixi116 – 1216
    Helixi122 – 1243
    Turni128 – 1314
    Beta strandi157 – 1604

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A73X-ray1.80A/B1-163[»]
    1A74X-ray2.20A/B1-163[»]
    1CYQX-ray1.93A/B2-163[»]
    1CZ0X-ray2.10A/B2-163[»]
    1EVWX-ray3.10A/B/C/D1-163[»]
    1EVXX-ray2.00A/B2-163[»]
    1IPPX-ray2.20A/B1-163[»]
    2O6MX-ray2.30A/B1-163[»]
    ProteinModelPortaliQ94702.
    SMRiQ94702. Positions 2-163.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ94702.

    Family & Domainsi

    Family and domain databases

    InterProiIPR008704. Endonuclease_Zinc-binding_loop.
    [Graphical view]
    PfamiPF05551. zf-His_Me_endon. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q94702-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALTNAQILA VIDSWEETVG QFPVITHHVP LGGGLQGTLH CYEIPLAAPY    50
    GVGFAKNGPT RWQYKRTINQ VVHRWGSHTV PFLLEPDNIN GKTCTASHLC 100
    HNTRCHNPLH LCWESLDDNK GRNWCPGPNG GCVHAVVCLR QGPLYGPGAT 150
    VAGPQQRGSH FVV 163
    Length:163
    Mass (Da):17,789
    Last modified:December 15, 1998 - v3
    Checksum:iA193B6F06B0D8EEF
    GO

    Sequence cautioni

    The sequence AAB65764.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38131 Genomic DNA. Translation: AAB65765.1.
    M38131 Genomic DNA. Translation: AAB65764.1. Different initiation.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38131 Genomic DNA. Translation: AAB65765.1 .
    M38131 Genomic DNA. Translation: AAB65764.1 . Different initiation.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A73 X-ray 1.80 A/B 1-163 [» ]
    1A74 X-ray 2.20 A/B 1-163 [» ]
    1CYQ X-ray 1.93 A/B 2-163 [» ]
    1CZ0 X-ray 2.10 A/B 2-163 [» ]
    1EVW X-ray 3.10 A/B/C/D 1-163 [» ]
    1EVX X-ray 2.00 A/B 2-163 [» ]
    1IPP X-ray 2.20 A/B 1-163 [» ]
    2O6M X-ray 2.30 A/B 1-163 [» ]
    ProteinModelPortali Q94702.
    SMRi Q94702. Positions 2-163.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2469. I-PpoI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q94702.

    Family and domain databases

    InterProi IPR008704. Endonuclease_Zinc-binding_loop.
    [Graphical view ]
    Pfami PF05551. zf-His_Me_endon. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum."
      Muscarella D.E., Ellison E.L., Ruoff B.M., Vogt V.M.
      Mol. Cell. Biol. 10:3386-3396(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Vogt V.M.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI."
      Flick K.E., Jurica M.S., Monnat R.J. Jr., Stoddard B.L.
      Nature 394:96-101(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
    4. Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS).
    5. "Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site."
      Galburt E.A., Chadsey M.S., Jurica M.S., Chevalier B.S., Erho D., Tang W., Monnat R.J. Jr., Stoddard B.L.
      J. Mol. Biol. 300:877-887(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiPPO1_PHYPO
    AccessioniPrimary (citable) accession number: Q94702
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 55 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3