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Q94702

- PPO1_PHYPO

UniProt

Q94702 - PPO1_PHYPO

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Protein
Intron-encoded endonuclease I-PpoI
Gene
N/A
Organism
Physarum polycephalum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.

Cofactori

Zinc.

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Intron homing

Keywords - Ligandi

Zinc

Protein family/group databases

REBASEi2469. I-PpoI.

Names & Taxonomyi

Protein namesi
Recommended name:
Intron-encoded endonuclease I-PpoI (EC:3.1.-.-)
Short name:
I-Ppo
OrganismiPhysarum polycephalum (Slime mold)
Taxonomic identifieri5791 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaMyxogastriaMyxogastromycetidaePhysariidaPhysaraceaePhysarum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 163163Intron-encoded endonuclease I-PpoI
PRO_0000192788Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1915
Beta strandi24 – 329
Beta strandi35 – 439
Beta strandi46 – 483
Beta strandi54 – 585
Beta strandi61 – 688
Beta strandi71 – 766
Turni77 – 793
Helixi80 – 823
Beta strandi83 – 853
Beta strandi87 – 893
Beta strandi92 – 987
Helixi108 – 1103
Beta strandi111 – 1155
Helixi116 – 1216
Helixi122 – 1243
Turni128 – 1314
Beta strandi157 – 1604

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A73X-ray1.80A/B1-163[»]
1A74X-ray2.20A/B1-163[»]
1CYQX-ray1.93A/B2-163[»]
1CZ0X-ray2.10A/B2-163[»]
1EVWX-ray3.10A/B/C/D1-163[»]
1EVXX-ray2.00A/B2-163[»]
1IPPX-ray2.20A/B1-163[»]
2O6MX-ray2.30A/B1-163[»]
ProteinModelPortaliQ94702.
SMRiQ94702. Positions 2-163.

Miscellaneous databases

EvolutionaryTraceiQ94702.

Family & Domainsi

Family and domain databases

InterProiIPR008704. Endonuclease_Zinc-binding_loop.
[Graphical view]
PfamiPF05551. zf-His_Me_endon. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q94702-1 [UniParc]FASTAAdd to Basket

« Hide

MALTNAQILA VIDSWEETVG QFPVITHHVP LGGGLQGTLH CYEIPLAAPY    50
GVGFAKNGPT RWQYKRTINQ VVHRWGSHTV PFLLEPDNIN GKTCTASHLC 100
HNTRCHNPLH LCWESLDDNK GRNWCPGPNG GCVHAVVCLR QGPLYGPGAT 150
VAGPQQRGSH FVV 163
Length:163
Mass (Da):17,789
Last modified:December 15, 1998 - v3
Checksum:iA193B6F06B0D8EEF
GO

Sequence cautioni

The sequence AAB65764.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38131 Genomic DNA. Translation: AAB65765.1.
M38131 Genomic DNA. Translation: AAB65764.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38131 Genomic DNA. Translation: AAB65765.1 .
M38131 Genomic DNA. Translation: AAB65764.1 . Different initiation.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A73 X-ray 1.80 A/B 1-163 [» ]
1A74 X-ray 2.20 A/B 1-163 [» ]
1CYQ X-ray 1.93 A/B 2-163 [» ]
1CZ0 X-ray 2.10 A/B 2-163 [» ]
1EVW X-ray 3.10 A/B/C/D 1-163 [» ]
1EVX X-ray 2.00 A/B 2-163 [» ]
1IPP X-ray 2.20 A/B 1-163 [» ]
2O6M X-ray 2.30 A/B 1-163 [» ]
ProteinModelPortali Q94702.
SMRi Q94702. Positions 2-163.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2469. I-PpoI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q94702.

Family and domain databases

InterProi IPR008704. Endonuclease_Zinc-binding_loop.
[Graphical view ]
Pfami PF05551. zf-His_Me_endon. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum."
    Muscarella D.E., Ellison E.L., Ruoff B.M., Vogt V.M.
    Mol. Cell. Biol. 10:3386-3396(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Vogt V.M.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI."
    Flick K.E., Jurica M.S., Monnat R.J. Jr., Stoddard B.L.
    Nature 394:96-101(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
  4. Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS).
  5. "Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site."
    Galburt E.A., Chadsey M.S., Jurica M.S., Chevalier B.S., Erho D., Tang W., Monnat R.J. Jr., Stoddard B.L.
    J. Mol. Biol. 300:877-887(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiPPO1_PHYPO
AccessioniPrimary (citable) accession number: Q94702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: April 16, 2014
This is version 54 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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