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Q94702 (PPO1_PHYPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intron-encoded endonuclease I-PpoI

Short name=I-Ppo
EC=3.1.-.-
OrganismPhysarum polycephalum (Slime mold)
Taxonomic identifier5791 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaMyxogastriaMyxogastromycetidaePhysariidaPhysaraceaePhysarum

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.

Cofactor

Zinc.

Subunit structure

Homodimer.

Sequence caution

The sequence AAB65764.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processIntron homing
   LigandZinc
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processintron homing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionendonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 163163Intron-encoded endonuclease I-PpoI
PRO_0000192788

Secondary structure

............................... 163
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q94702 [UniParc].

Last modified December 15, 1998. Version 3.
Checksum: A193B6F06B0D8EEF

FASTA16317,789
        10         20         30         40         50         60 
MALTNAQILA VIDSWEETVG QFPVITHHVP LGGGLQGTLH CYEIPLAAPY GVGFAKNGPT 

        70         80         90        100        110        120 
RWQYKRTINQ VVHRWGSHTV PFLLEPDNIN GKTCTASHLC HNTRCHNPLH LCWESLDDNK 

       130        140        150        160 
GRNWCPGPNG GCVHAVVCLR QGPLYGPGAT VAGPQQRGSH FVV 

« Hide

References

[1]"Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum."
Muscarella D.E., Ellison E.L., Ruoff B.M., Vogt V.M.
Mol. Cell. Biol. 10:3386-3396(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Vogt V.M.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI."
Flick K.E., Jurica M.S., Monnat R.J. Jr., Stoddard B.L.
Nature 394:96-101(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[4]"A novel endonuclease mechanism directly visualized for I-PpoI."
Galburt E.A., Chevalier B., Tang W., Jurica M.S., Flick K.E., Monnat R.J. Jr., Stoddard B.L.
Nat. Struct. Biol. 6:1096-1099(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS).
[5]"Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site."
Galburt E.A., Chadsey M.S., Jurica M.S., Chevalier B.S., Erho D., Tang W., Monnat R.J. Jr., Stoddard B.L.
J. Mol. Biol. 300:877-887(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M38131 Genomic DNA. Translation: AAB65765.1.
M38131 Genomic DNA. Translation: AAB65764.1. Different initiation.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1A73X-ray1.80A/B1-163[»]
1A74X-ray2.20A/B1-163[»]
1CYQX-ray1.93A/B2-163[»]
1CZ0X-ray2.10A/B2-163[»]
1EVWX-ray3.10A/B/C/D1-163[»]
1EVXX-ray2.00A/B2-163[»]
1IPPX-ray2.20A/B1-163[»]
2O6MX-ray2.30A/B1-163[»]
ProteinModelPortalQ94702.
SMRQ94702. Positions 2-163.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2469. I-PpoI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008704. Endonuclease_Zinc-binding_loop.
[Graphical view]
PfamPF05551. zf-His_Me_endon. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ94702.

Entry information

Entry namePPO1_PHYPO
AccessionPrimary (citable) accession number: Q94702
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: April 16, 2014
This is version 54 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references