Reviewed, UniProtKB/Swiss-Prot Q94655 (GSHR_PLAFK)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

GR2

Organism

Plasmodium falciparum (isolate K1 / Thailand)

Taxonomic identifier

5839 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 500

499

Glutathione reductase

Regions

Nucleotide binding

32 – 40

9

FAD By similarity

Sites

Active site

485

1

Proton acceptor By similarity

Amino acid modifications

Disulfide bond

40 ↔ 45

Redox-active By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

500

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Helix

12 – 23

12

Beta strand

28 – 35

8

Helix

38 – 42

5

Helix

45 – 63

19

Helix

64 – 67

4

Beta strand

69 – 75

7

Helix

77 – 101

25

Beta strand

105 – 109

5

Beta strand

142 – 147

6

Helix

161 – 163

3

Helix

167 – 170

4

Beta strand

177 – 182

6

Helix

186 – 196

11

Turn

197 – 199

3

Beta strand

201 – 205

5

Beta strand

207 – 211

5

Helix

217 – 229

13

Beta strand

233 – 235

3

Beta strand

240 – 247

8

Beta strand

251 – 255

5

Beta strand

260 – 269

10

Turn

276 – 279

4

Turn

283 – 286

4

Beta strand

289 – 292

4

Beta strand

294 – 296

3

Beta strand

303 – 309

7

Beta strand

314 – 316

3

Helix

354 – 369

16

Beta strand

383 – 385

3

Beta strand

391 – 395

5

Helix

398 – 404

7

Helix

407 – 409

3

Beta strand

410 – 417

8

Helix

420 – 422

3

Helix

429 – 431

3

Beta strand

435 – 442

8

Turn

443 – 446

4

Beta strand

447 – 455

9

Helix

458 – 470

13

Helix

475 – 479

5

Helix

491 – 494

4

Sequences

	Sequence		Length	Mass (Da)
	Q94655-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: AB2299144002FCD3	FASTA	500	56,561
	10 20 30 40 50 60 MVYDLIVIGG GSGGMAAARR AARHNAKVAL VEKSRLGGTC VNVGCVPKKI MFNAASVHDI 70 80 90 100 110 120 LENSRHYGFD TKFSFNLPLL VERRDKYIQR LNNIYRQNLS KDKVDLYEGT ASFLSENRIL 130 140 150 160 170 180 IKGTKDNNNK DNGPLNEEIL EGRNILIAVG NKPVFPPVKG IENTISSDEF FNIKESKKIG 190 200 210 220 230 240 IVGSGYIAVE LINVIKRLGI DSYIFARGNR ILRKFDESVI NVLENDMKKN NINIVTFADV 250 260 270 280 290 300 VEIKKVSDKN LSIHLSDGRI YEHFDHVIYC VGRSPDTENL KLEKLNVETN NNYIVVDENQ 310 320 330 340 350 360 RTSVNNIYAV GDCCMVKKSK EIEDLNLLKL YNEERYLNKK ENVTEDIFYN VQLTPVAINA 370 380 390 400 410 420 GRLLADRLFL KKTRKTNYKL IPTVIFSHPP IGTIGLSEEA AIQIYGKENV KIYESKFTNL 430 440 450 460 470 480 FFSVYDIEPE LKEKTYLKLV CVGKDELIKG LHIIGLNADE IVQGFAVALK MNATKKDFDE 490 500 TIPIHPTAAE EFLTLQPWMK

References

[1]	"Molecular cloning and characterization of a putative glutathione reductase gene, the PfGR2 gene, from Plasmodium falciparum." Faerber P.M., Becker K., Mueller S. Eur. J. Biochem. 239:655-661(1996) [PubMed: 8774709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Glutathione reductase and glutamate dehydrogenase of Plasmodium falciparum, the causative agent of tropical malaria." Krauth-Siegel R.L., Muller J.G., Lottspeich F., Schirmer R.H. Eur. J. Biochem. 235:345-350(1996) [PubMed: 8631352] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16, CHARACTERIZATION.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X93462 Genomic DNA. Translation: CAA63747.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ONF	X-ray	2.60	A	1-500	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR000815. Hg_reductase.
IPR001100. Pyr_nuc-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD_bd.
[Graphical view]

Gene3D

G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.

Pfam

PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]

PRINTS

PR00368. FADPNR.
PR00945. HGRDTASE.
PR00411. PNDRDTASEI.

ProDom

PD000139. FAD_pyr_redox. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

GSHR_PLAFK

Accession

Primary (citable) accession number: Q94655

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	September 2, 2008
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)