Reviewed, UniProtKB/Swiss-Prot Q945S8 (ASHH3_ARATH)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase ASHH3
    EC=2.1.1.43
Alternative name(s):
    ASH1 homolog 3
    Protein SET DOMAIN GROUP 7

Gene names

Name:	ASHH3
Synonyms:	SDG7, SET7
Ordered Locus Names:	At2g44150
ORF Names:	F6E13.28

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Histone methyltransferase By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus By similarity. Centromere By similarity. Note: Associates with centromeric constitutive heterochromatin By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 1 AWS domain. Contains 1 post-SET domain. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Centromere Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Histone-lysine N-methyltransferase ASHH3		PRO_0000233372
Regions
	Domain	63 – 114	52	AWS
	Domain	115 – 237	123	SET
	Domain	239 – 255	17	Post-SET
Experimental info
	Sequence conflict	55	1	K → E in AAL01111. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q945S8-1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: BA4CB48ACF54DFBF	FASTA	363	41,286
	10 20 30 40 50 60 MPASKKISDR NHLGQVFDKL LNQIGESEEF ELPEWLNKGK PTPYIFIRRN IYLTKKVKRR 70 80 90 100 110 120 VEDDGIFCSC SSSSPGSSST VCGSNCHCGM LFSSCSSSCK CGSECNNKPF QQRHVKKMKL 130 140 150 160 170 180 IQTEKCGSGI VAEEEIEAGE FIIEYVGEVI DDKTCEERLW KMKHRGETNF YLCEITRDMV 190 200 210 220 230 240 IDATHKGNKS RYINHSCNPN TQMQKWIIDG ETRIGIFATR GIKKGEHLTY DYQFVQFGAD 250 260 270 280 290 300 QDCHCGAVGC RRKLGVKPSK PKIASDEAFN LVAHELAQTL PKVHQNGLVN RHIDAGKSWN 310 320 330 340 350 360 NLSQRDTCSR NCIGVVIRLS RPTSDRCFGL VRHFDEYSRK HSVMFEDGVT EFVDMSREDW EIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed: 11691919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-135, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004005 Genomic DNA. Translation: AAC23419.2. AY045886 mRNA. Translation: AAK76560.1. AY091447 mRNA. Translation: AAM14386.1. AF408060 mRNA. Translation: AAL01111.1.
IPI	IPI00525260.
PIR	T00695.
RefSeq	NP_566010.1.
UniGene	At.25522
3D structure databases
SMR	Q945S8. Positions 29-255.
ModBase	Search...
Proteomic databases
PRIDE	Q945S8.
Genome annotation databases
GeneID	819021.
GenomeReviews	Gene locus AT2G44150 in contig CT485783_GR.
KEGG	ath:AT2G44150.
NMPDR	fig|3702.1.peg.11588.
Organism-specific databases
TAIR	At2g44150.
Phylogenomic databases
eggNOG	KOG1081.
HOGENOM	HBG602412.
InParanoid	Q945S8.
OMA	KTCEERL.
Enzyme and pathway databases
BRENDA	2.1.1.43. 302.
Gene expression databases
Genevestigator	Q945S8.
GermOnline	AT2G44150. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006560. AWS. IPR003616. Post-SET_dom. IPR001214. SET_dom. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00570. AWS. 1 hit. SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51215. AWS. 1 hit. PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASHH3_ARATH

Accession

Primary (citable) accession number: Q945S8
Secondary accession number(s): O80584, Q94AQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	February 9, 2010
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)