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Q945K7

- IDH5_ARATH

UniProt

Q945K7 - IDH5_ARATH

Protein

Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial

Gene

IDH5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.By similarity

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei127 – 1271SubstrateBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Sitei165 – 1651Critical for catalysisBy similarity
    Sitei212 – 2121Critical for catalysisBy similarity
    Metal bindingi245 – 2451Magnesium or manganeseBy similarity
    Binding sitei245 – 2451SubstrateBy similarity
    Metal bindingi269 – 2691Magnesium or manganeseBy similarity
    Metal bindingi273 – 2731Magnesium or manganeseBy similarity

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. isocitrate dehydrogenase (NAD+) activity Source: TAIR
    3. magnesium ion binding Source: InterPro
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: TAIR

    GO - Biological processi

    1. isocitrate metabolic process Source: TAIR
    2. tricarboxylic acid cycle Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:AT5G03290-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    IDH-V
    Isocitric dehydrogenase 5
    NAD(+)-specific ICDH 5
    Gene namesi
    Name:IDH5
    Ordered Locus Names:At5g03290
    ORF Names:F12E4_20, MOK16.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G03290.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4444MitochondrionSequence AnalysisAdd
    BLAST
    Chaini45 – 374330Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrialPRO_0000271291Add
    BLAST

    Proteomic databases

    PaxDbiQ945K7.
    PRIDEiQ945K7.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    GenevestigatoriQ945K7.

    Interactioni

    Subunit structurei

    Heterooligomer of catalytic and regulatory subunits.

    Protein-protein interaction databases

    BioGridi17160. 2 interactions.
    IntActiQ945K7. 1 interaction.
    STRINGi3702.AT5G03290.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ945K7.
    SMRiQ945K7. Positions 40-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    InParanoidiQ945K7.
    KOiK00030.
    OMAiTPIAWEP.
    PhylomeDBiQ945K7.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q945K7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMAANLARR LIGNRSTQIL GAVNSSSGAA SSVARAFCSS TTPITATLFP    50
    GDGIGPEIAE SVKKVFTTAG VPIEWEEHYV GTEIDPRTQS FLTWESLESV 100
    RRNKVGLKGP MATPIGKGHR SLNLTLRKEL NLYANVRPCY SLPGYKTRYD 150
    DVDLITIREN TEGEYSGLEH QVVRGVVESL KIITRQASLR VAEYAFLYAK 200
    THGRERVSAI HKANIMQKTD GLFLKCCREV AEKYPEITYE EVVIDNCCMM 250
    LVKNPALFDV LVMPNLYGDI ISDLCAGLVG GLGLTPSCNI GEDGVALAEA 300
    VHGSAPDIAG KNLANPTALL LSGVMMLRHL KFNEQAEQIH SAIINTIAEG 350
    KYRTADLGGS STTTEFTKAI CDHL 374
    Length:374
    Mass (Da):40,625
    Last modified:December 1, 2001 - v1
    Checksum:i723D0150C360F7A1
    GO

    Sequence cautioni

    The sequence BAB08389.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAB83285.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005240 Genomic DNA. Translation: BAB08389.1. Different initiation.
    AL162751 Genomic DNA. Translation: CAB83285.1. Different initiation.
    CP002688 Genomic DNA. Translation: AED90581.1.
    AF412100 mRNA. Translation: AAL06553.1.
    AY099823 mRNA. Translation: AAM20674.1.
    BT008460 mRNA. Translation: AAP37819.1.
    PIRiT48350.
    RefSeqiNP_568113.1. NM_120407.5.
    UniGeneiAt.25268.

    Genome annotation databases

    EnsemblPlantsiAT5G03290.1; AT5G03290.1; AT5G03290.
    GeneIDi831884.
    KEGGiath:AT5G03290.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005240 Genomic DNA. Translation: BAB08389.1 . Different initiation.
    AL162751 Genomic DNA. Translation: CAB83285.1 . Different initiation.
    CP002688 Genomic DNA. Translation: AED90581.1 .
    AF412100 mRNA. Translation: AAL06553.1 .
    AY099823 mRNA. Translation: AAM20674.1 .
    BT008460 mRNA. Translation: AAP37819.1 .
    PIRi T48350.
    RefSeqi NP_568113.1. NM_120407.5.
    UniGenei At.25268.

    3D structure databases

    ProteinModelPortali Q945K7.
    SMRi Q945K7. Positions 40-374.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17160. 2 interactions.
    IntActi Q945K7. 1 interaction.
    STRINGi 3702.AT5G03290.1-P.

    Proteomic databases

    PaxDbi Q945K7.
    PRIDEi Q945K7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G03290.1 ; AT5G03290.1 ; AT5G03290 .
    GeneIDi 831884.
    KEGGi ath:AT5G03290.

    Organism-specific databases

    TAIRi AT5G03290.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    InParanoidi Q945K7.
    KOi K00030.
    OMAi TPIAWEP.
    PhylomeDBi Q945K7.

    Enzyme and pathway databases

    BioCyci MetaCyc:AT5G03290-MONOMER.

    Gene expression databases

    Genevestigatori Q945K7.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    6. "Characterization of a mutation in the IDH-II subunit of the NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana."
      Lin M., Behal R.H., Oliver D.J.
      Plant Sci. 166:983-988(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: GENE FAMILY.
    7. "Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs."
      Lemaitre T., Hodges M.
      Plant Cell Physiol. 47:634-643(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiIDH5_ARATH
    AccessioniPrimary (citable) accession number: Q945K7
    Secondary accession number(s): Q9LZG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3