Reviewed,
UniProtKB/Swiss-Prot Q945K7 (IDH5_ARATH)
Last modified
February 9, 2010.
Version 54.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial EC=1.1.1.41 Alternative name(s): Isocitric dehydrogenase 5 NAD(+)-specific ICDH 5 IDH-V | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 374 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Performs an essential role in the oxidative function of the citric acid cycle By similarity. |
| Catalytic activity | Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH. |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Subunit structure | Heteroligomer of catalytic and regulatory subunits. |
| Subcellular location | |
| Tissue specificity | Ubiquitous. Ref.6 |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from direct assay. Source: TAIR mitochondrion Ref.4Inferred from direct assay. Source: TAIR |
| Molecular function | ATP binding Inferred from direct assay. Source: TAIR NAD or NADH bindingInferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 44 | 44 | Mitochondrion Potential | ||||||
| Chain | 45 – 374 | 330 | Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial | PRO_0000271291 | |||||
Sites | |||||||||
| Metal binding | 245 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 269 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 273 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 127 | 1 | Substrate By similarity | ||||||
| Binding site | 137 | 1 | Substrate By similarity | ||||||
| Binding site | 158 | 1 | Substrate By similarity | ||||||
| Binding site | 245 | 1 | Substrate By similarity | ||||||
| Site | 165 | 1 | Critical for catalysis By similarity | ||||||
| Site | 212 | 1 | Critical for catalysis By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. Fransz P.F.Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [5] | "Characterization of a mutation in the IDH-II subunit of the NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana." Lin M., Behal R.H., Oliver D.J. Plant Sci. 166:983-988(2004) [Agricola: IND43633651] Cited for: GENE FAMILY. |
| [6] | "Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs." Lemaitre T., Hodges M. Plant Cell Physiol. 47:634-643(2006) [PubMed: 16527867] [Abstract] Cited for: TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB005240 Genomic DNA. Translation: BAB08389.1. Different initiation. AL162751 Genomic DNA. Translation: CAB83285.1. Different initiation. AF412100 mRNA. Translation: AAL06553.1. AY099823 mRNA. Translation: AAM20674.1. BT008460 mRNA. Translation: AAP37819.1. |
| IPI | IPI00530201. |
| PIR | T48350. |
| RefSeq | NP_568113.1. |
| UniGene | At.25268 Rra.8263 Rsa.4566 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1X0L based on UniProtKB Q8RQU4. |
| SMR | Q945K7. Positions 41-374. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q945K7. |
Proteomic databases | |
| PRIDE | Q945K7. |
Genome annotation databases | |
| GeneID | 831884. |
| GenomeReviews | Gene locus AT5G03290 in contig BA000015_GR. |
| KEGG | ath:AT5G03290. |
| NMPDR | fig|3702.1.peg.22410. |
Organism-specific databases | |
| TAIR | At5g03290. |
Phylogenomic databases | |
| eggNOG | KOG0785. |
| HOGENOM | HBG518924. |
| InParanoid | Q945K7. |
| OMA | RRNKVGL. |
| PhylomeDB | Q945K7. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.41. 302. |
Gene expression databases | |
| ArrayExpress | Q945K7. |
| Genevestigator | Q945K7. |
Family and domain databases | |
| InterPro | IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11835. IDH_IMDH_dimeric. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00175. mito_nad_idh. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IDH5_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q945K7 Secondary accession number(s): Q9LZG1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


