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Protein

Protein DETOXIFICATION 47, chloroplastic

Gene

DTX47

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as a multidrug and toxin extrusion transporter in the export of salicylic acid (SA) from the chloroplast to the cytoplasm (PubMed:23757404). Plays an essential function in plant defense via the pathogen-induced salicylic acid (SA) accumulation (PubMed:11826312, PubMed:24594657). Acts also as a key component of the Age-related resistance (ARR) pathway (PubMed:11884688, PubMed:19694953, PubMed:24594657).5 Publications

GO - Molecular functioni

GO - Biological processi

  • defense response Source: TAIR
  • defense response to bacterium Source: TAIR
  • drug transmembrane transport Source: GO_Central
  • innate immune response Source: UniProtKB
  • negative regulation of defense response Source: TAIR
  • response to nematode Source: TAIR
  • response to salicylic acid Source: UniProtKB
  • salicylic acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity, Plant defense, Transport

Protein family/group databases

TCDBi2.A.66.1.11. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DETOXIFICATION 47, chloroplastic1 Publication
Short name:
AtDTX471 Publication
Alternative name(s):
Multidrug and toxic compound extrusion protein 47Curated
Short name:
MATE protein 47Curated
Protein ENHANCED DISEASE SUSCEPTIBILITY 51 Publication
Short name:
Protein EDS51 Publication
Protein IMPORTANT FOR THE ARR PATHWAY 11 Publication
Short name:
Protein IAP11 Publication
Protein SALICYLIC ACID INDUCTION DEFICIENT 11 Publication
Short name:
Protein SID11 Publication
Protein SUSCEPTIBLE TO CORONATINE-DEFICIENT PST DC3000 3
Short name:
Protein SCORD31 Publication
Gene namesi
Name:DTX471 Publication
Synonyms:EDS51 Publication, IAP11 Publication, SCORD31 Publication, SID11 Publication
Ordered Locus Names:At4g39030Imported
ORF Names:F19H22.130Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G39030.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST
Transmembranei135 – 15521HelicalSequence analysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Transmembranei228 – 24821HelicalSequence analysisAdd
BLAST
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Transmembranei319 – 33921HelicalSequence analysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Transmembranei406 – 42621HelicalSequence analysisAdd
BLAST
Transmembranei443 – 46321HelicalSequence analysisAdd
BLAST
Transmembranei472 – 49221HelicalSequence analysisAdd
BLAST
Transmembranei497 – 51721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast envelope Source: UniProtKB
  • chloroplast membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrion Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

Enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression (PubMed:9090877). No salicylic acid (SA) accumulation after pathogen inoculation and more susceptibility to both virulent and avirulent forms of Pseudomonas syringae and Peronospora parasitica (PubMed:10449575, PubMed:24594657). Age-related resistance (ARR)-defective (PubMed:11884688, PubMed:19694953). Defect in stomatal response during bacterial infection (PubMed:21998587).6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030ChloroplastSequence analysisAdd
BLAST
Chaini31 – 543513Protein DETOXIFICATION 47, chloroplasticPRO_0000164259Add
BLAST

Proteomic databases

PaxDbiQ945F0.
PRIDEiQ945F0.

PTM databases

SwissPalmiQ945F0.

Expressioni

Tissue specificityi

Preferentially expressed in the epidermal cells.1 Publication

Inductioni

By salicylic acid, UV-C light and pathogens.1 Publication

Gene expression databases

ExpressionAtlasiQ945F0. baseline and differential.
GenevisibleiQ945F0. AT.

Interactioni

Protein-protein interaction databases

BioGridi15338. 26 interactions.
STRINGi3702.AT4G39030.1.

Structurei

3D structure databases

ProteinModelPortaliQ945F0.
SMRiQ945F0. Positions 95-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili55 – 9440Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi78 – 869Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
HOGENOMiHOG000029977.
InParanoidiQ945F0.
OMAiAMGMWIC.
PhylomeDBiQ945F0.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q945F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKSQRLTL FSPLLSKTRR IPVNSHQTLV AESVITRRTL GAITATPSFH
60 70 80 90 100
KNPVVIRRRI KLERVTRNCV RIDREIDEEE EEEEKERGDL VKQSIWEQMK
110 120 130 140 150
EIVKFTGPAM GMWICGPLMS LIDTVVIGQG SSIELAALGP GTVLCDHMSY
160 170 180 190 200
VFMFLSVATS NMVATSLAKQ DKKEAQHQIS VLLFIGLVCG LMMLLLTRLF
210 220 230 240 250
GPWAVTAFTR GKNIEIVPAA NKYIQIRGLA WPFILVGLVA QSASLGMKNS
260 270 280 290 300
WGPLKALAAA TIINGLGDTI LCLFLGQGIA GAAWATTASQ IVSAYMMMDS
310 320 330 340 350
LNKEGYNAYS FAIPSPQELW KISALAAPVF ISIFSKIAFY SFIIYCATSM
360 370 380 390 400
GTHVLAAHQV MAQTYRMCNV WGEPLSQTAQ SFMPEMLYGA NRNLPKARTL
410 420 430 440 450
LKSLMIIGAT LGLVLGVIGT AVPGLFPGVY THDKVIISEM HRLLIPFFMA
460 470 480 490 500
LSALPMTVSL EGTLLAGRDL KFVSSVMSSS FIIGCLTLMF VTRSGYGLLG
510 520 530 540
CWFVLVGFQW GRFGLYLRRL LSPGGILNSD GPSPYTVEKI KSI
Length:543
Mass (Da):59,528
Last modified:December 1, 2001 - v1
Checksum:iDF2EEFE0942A51C5
GO

Sequence cautioni

The sequence CAB38823.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80566.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF416569 mRNA. Translation: AAL27003.1.
AL035679 Genomic DNA. Translation: CAB38823.1. Sequence problems.
AL161594 Genomic DNA. Translation: CAB80566.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE87009.1.
AK175482 mRNA. Translation: BAD43245.1.
AK176074 mRNA. Translation: BAD43837.1.
AK176272 mRNA. Translation: BAD44035.1.
AK176334 mRNA. Translation: BAD44097.1.
RefSeqiNP_195614.2. NM_120063.5.
UniGeneiAt.2846.
At.66625.

Genome annotation databases

EnsemblPlantsiAT4G39030.1; AT4G39030.1; AT4G39030.
GeneIDi830058.
GrameneiAT4G39030.1; AT4G39030.1; AT4G39030.
KEGGiath:AT4G39030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF416569 mRNA. Translation: AAL27003.1.
AL035679 Genomic DNA. Translation: CAB38823.1. Sequence problems.
AL161594 Genomic DNA. Translation: CAB80566.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE87009.1.
AK175482 mRNA. Translation: BAD43245.1.
AK176074 mRNA. Translation: BAD43837.1.
AK176272 mRNA. Translation: BAD44035.1.
AK176334 mRNA. Translation: BAD44097.1.
RefSeqiNP_195614.2. NM_120063.5.
UniGeneiAt.2846.
At.66625.

3D structure databases

ProteinModelPortaliQ945F0.
SMRiQ945F0. Positions 95-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15338. 26 interactions.
STRINGi3702.AT4G39030.1.

Protein family/group databases

TCDBi2.A.66.1.11. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

SwissPalmiQ945F0.

Proteomic databases

PaxDbiQ945F0.
PRIDEiQ945F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G39030.1; AT4G39030.1; AT4G39030.
GeneIDi830058.
GrameneiAT4G39030.1; AT4G39030.1; AT4G39030.
KEGGiath:AT4G39030.

Organism-specific databases

TAIRiAT4G39030.

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
HOGENOMiHOG000029977.
InParanoidiQ945F0.
OMAiAMGMWIC.
PhylomeDBiQ945F0.

Miscellaneous databases

PROiQ945F0.

Gene expression databases

ExpressionAtlasiQ945F0. baseline and differential.
GenevisibleiQ945F0. AT.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family."
    Nawrath C., Heck S., Parinthawong N., Metraux J.-P.
    Plant Cell 14:275-286(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression."
    Rogers E.E., Ausubel F.M.
    Plant Cell 9:305-316(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT EDS5-1, DISRUPTION PHENOTYPE.
  6. "Salicylic acid induction-deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation."
    Nawrath C., Metraux J.P.
    Plant Cell 11:1393-1404(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT SID1, DISRUPTION PHENOTYPE.
  7. "Functional cloning and characterization of a plant efflux carrier for multidrug and heavy metal detoxification."
    Li L., He Z., Pandey G.K., Tsuchiya T., Luan S.
    J. Biol. Chem. 277:5360-5368(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Age-related resistance in Arabidopsis is a developmentally regulated defense response to Pseudomonas syringae."
    Kus J.V., Zaton K., Sarkar R., Cameron R.K.
    Plant Cell 14:479-490(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "The multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily."
    Hvorup R.N., Winnen B., Chang A.B., Jiang Y., Zhou X.F., Saier M.H. Jr.
    Eur. J. Biochem. 270:799-813(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  10. "Forward and reverse genetics to identify genes involved in the age-related resistance response in Arabidopsis thaliana."
    Carviel J.L., Al-Daoud F., Neumann M., Mohammad A., Provart N.J., Moeder W., Yoshioka K., Cameron R.K.
    Mol. Plant Pathol. 10:621-634(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT IAP1-1, DISRUPTION PHENOTYPE, FUNCTION.
  11. "A genetic screen reveals Arabidopsis stomatal and/or apoplastic defenses against Pseudomonas syringae pv. tomato DC3000."
    Zeng W., Brutus A., Kremer J.M., Withers J.C., Gao X., Jones A.D., He S.Y.
    PLoS Pathog. 7:E1002291-E1002291(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT SCORD3, DISRUPTION PHENOTYPE.
  12. "Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5."
    Serrano M., Wang B., Aryal B., Garcion C., Abou-Mansour E., Heck S., Geisler M., Mauch F., Nawrath C., Metraux J.P.
    Plant Physiol. 162:1815-1821(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  13. "Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana."
    Yamasaki K., Motomura Y., Yagi Y., Nomura H., Kikuchi S., Nakai M., Shiina T.
    Plant Signal. Behav. 8:E23603-E23603(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  14. "Investigation of intercellular salicylic acid accumulation during compatible and incompatible Arabidopsis-pseudomonas syringae interactions using a fast neutron-generated mutant allele of EDS5 identified by genetic mapping and whole-genome sequencing."
    Carviel J.L., Wilson D.C., Isaacs M., Carella P., Catana V., Golding B., Weretilnyk E.A., Cameron R.K.
    PLoS ONE 9:E88608-E88608(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiDTX47_ARATH
AccessioniPrimary (citable) accession number: Q945F0
Secondary accession number(s): Q67ZP3, Q9SVJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: December 1, 2001
Last modified: February 17, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.